动物营养学报 >

2021 , Vol. 33 >Issue 5: 2875 - 2886

DOI: https://doi.org/10.3969/j.issn.1006-267x.2021.05.045

特种经济动物营养与饲料 SPECIAL ECONOMIC ANIMAL NUTRITION AND FEED

饲粮不同比例生姜秸秆替代花生秧对莱芜黑兔胃肠道发育、盲肠发酵和盲肠微生物区系的影响

  • 孙海涛 ,
  • 王勇 ,
  • 刘公言 ,
  • 徐云华 ,
  • 赵桂省 ,
  • 薛海鹏 ,
  • 李萌
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  • 1. 山东省农业科学院畜牧兽医研究所, 山东省畜禽疫病防治与繁育重点实验室, 济南 250100;
    2. 山东省济南市畜牧技术推广站, 济南 250100;
    3. 济南市莱芜黑兔种业科技有限公司, 济南 250100
孙海涛(1980-),男,山东郓城人,副研究员,硕士,主要从事家兔生产相关研究。E-mail:wwww8888@163.com

收稿日期: 2020-11-06

  网络出版日期: 2021-05-14

基金资助

山东省现代农业产业技术体系特种经济动物创新团队建设项目（SDAIT-21）；山东省农业重大应用技术创新兔舍环境测控设备及大数据分析应用平台项目（SF1805301301）；山东省农业良种工程（南种北繁）项目（2017LZN008）

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Effects of Different Ratios of Ginger Straw Substituted for Peanut Seedling in Diets on Gastrointestinal Development, Cecal Fermentation and Cecal Microflora of Laiwu Black Rabbits

  • SUN Haitao ,
  • WANG Yong ,
  • LIU Gongyan ,
  • XU Yunhua ,
  • ZHAO Guisheng ,
  • XUE Haipeng ,
  • LI Meng
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  • 1. Shandong Key Laboratory of Animal Disease Control and Breeding, Animal Husbandry and Veterinary Institute, Shandong Academy of Agricultural Sciences, Jinan 250100, China;
    2. Shandong Province Jinan Animal Husbandry Technology Extension Station, Jinan 250100, China;
    3. Jinan Laiwu Black Rabbit Seed Industry Technology Co., Ltd., Jinan 250100, China

Received date: 2020-11-06

  Online published: 2021-05-14

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摘要

本试验旨在研究饲粮不同比例生姜秸秆替代花生秧对莱芜黑兔胃肠道发育、小肠组织形态结构、盲肠发酵和盲肠内容物微生物区系的影响。试验选取45日龄育肥期莱芜黑兔160只，随机分为4组，分别饲喂以生姜秸秆替代0（A组）、5%（B组）、10%（C组）和15%（D组）花生秧的全价配合饲料，每组5个重复，每个重复8只家兔。预试期7 d，正试期47 d。结果表明：1）饲粮不同比例生姜秸秆替代花生秧对莱芜黑兔胃相对重量、胃内容物相对重量和大肠内容物相对重量有显著影响（P<0.05），其中C组和D组的胃相对重量显著高于A组和B组（P<0.05），D组胃内容物相对重量显著高于其他各组（P<0.05），B组、C组和D组大肠内容物相对重量显著高于A组（P<0.05）。2）饲粮不同比例生姜秸秆替代花生秧对莱芜黑兔小肠肌层厚度有显著影响（P<0.05），D组显著高于其他各组（P<0.05）。3）饲粮不同比例生姜秸秆替代花生秧对莱芜黑兔盲肠内容物中乙酸、丙酸和丁酸含量以及乙酸/（丙酸+丁酸）有显著影响（P<0.05），其中D组乙酸含量以及乙酸/（丙酸+丁酸）显著高于其他各组（P<0.05），丙酸和丁酸含量显著低于A组和B组（P<0.05）。4）莱芜黑兔盲肠内容物微生物组成在门水平上占据主导地位的主要有厚壁菌门（Firmicutes）、拟杆菌门（Bacteroidetes）、软壁菌门（Tenericutes），Shannon指数在D组与A组之间存在显著差异（P<0.05），表明高比例生姜秸秆替代花生秧能够影响盲肠内容物微生物区系。综上所述，饲粮适宜比例（5%~10%）的生姜秸秆替代花生秧对莱芜黑兔的胃肠道发育、小肠组织形态结构、盲肠发酵和盲肠内容物微生物区系无明显不良影响，生姜秸秆可用作家兔粗饲料原料在生产上使用。

关键词： 生姜秸秆; 莱芜黑兔; 胃肠道发育; 肠道形态结构; 盲肠发酵; 微生物区系

本文引用格式

孙海涛 , 王勇 , 刘公言 , 徐云华 , 赵桂省 , 薛海鹏 , 李萌 . 饲粮不同比例生姜秸秆替代花生秧对莱芜黑兔胃肠道发育、盲肠发酵和盲肠微生物区系的影响[J]. 动物营养学报, 2021 , 33(5) : 2875 -2886 . DOI: 10.3969/j.issn.1006-267x.2021.05.045

Abstract

This experiment was conducted to investigate the effects of different ratios of ginger straw substituted for peanut seedling in diets on gastrointestinal development, histological structure of small intestine, cecal fermentation and cecal microflora of Laiwu black rabbits. A total of 160 fattening Laiwu black rabbits at 45 days of age were randomly divided into 4 groups with 5 replicates in each group and each replicate contained 8 rabbits. Rabbits in the 4 groups were fed diets with 0 (group A), 5% (group B), 10% (group C) and 15% (group D) ginger straws replacing peanut seedlings diets, respectively. The trial lasted for 7 days for adaptation, and 47 days for test. The results showed as follows:1) different ratios of ginger straw substituted for peanut seedling in diets had significant effects on the relative weight of stomach, stomach contents and large intestine contents (P<0.05), among which the relative weight of stomach in group C and group D was significantly higher than that in group B and group A (P<0.05); the relative weight of gastric content in group D was significantly higher than that in other groups (P<0.05); the relative weight of large intestine content in groups B, C and D was significantly higher than that in group A (P<0.05). 2) Different ratios of ginger straw substituted for peanut seedling in diets had significant effects on the thickness of intestinal muscle layer (P<0.05), and group D was significantly higher than others groups (P<0.05). 3) The contents of acetic acid, propionic acid, butyric acid and acetic acid/(propionic acid+butyric acid) in cecal contents of Laiwu black rabbits were significantly different (P<0.05). The acetic acid content and acetic acid/(propionic acid+butyric acid) in group D were significantly higher than those in other groups (P<0.05), while the contents of propionic acid and butyric acid were significantly lower than those in groups A and B (P<0.05). 4) The dominant microbial composition of cecal contents in Laiwu black rabbits was Firmicutes, Bacteroidetes, Tenericutes, and there were significant differences in Shannon index between group D and group A (P<0.05), indicating that high level of ginger straw instead of peanut seedling could affect the microflora of cecal contents. In conclusion, replacing peanut seedling with the optimum level (5% to 10%) of ginger straw in diets has no obvious adverse effects on gastrointestinal development, intestinal histological structure, cecal fermentation and cecal content microflora of Laiwu black rabbits. Therefore, ginger straw can be used as a raw material for rabbit feed.

Key words： ginger straws; Laiwu black rabbits; gastrointestinal development; intestine histological structure; cecal fermentation; intestinal microflora

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