动物营养学报 >

2021 , Vol. 33 >Issue 5: 2927 - 2938

DOI: https://doi.org/10.3969/j.issn.1006-267x.2021.05.050

分子与细胞营养 MOLECULAR AND CELLULAR NUTRITION

γ-氨基丁酸对热应激雏鸡胰腺组织结构、抗氧化能力、消化酶活性及细胞凋亡的影响

  • 温静 ,
  • 余哲琪 ,
  • 田佳迎 ,
  • 陈忠
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  • 海南师范大学热带岛屿生态学教育部重点实验室, 海口 571158
温静(1993-),女,山西朔州人,硕士研究生,研究方向为动物营养生理学。E-mail:1138231102@qq.com

收稿日期: 2020-10-30

  网络出版日期: 2021-05-14

基金资助

国家自然科学基金项目（31060312，31560680）

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Effects of γ-Aminobutyric Acid on Pancreatic Tissue Structure, Antioxidant Capacity, Digestive Enzyme Activities and Cell Apoptosis of Heat-Stressed Chicks

  • WEN Jing ,
  • YU Zheqi ,
  • TIAN Jiaying ,
  • CHEN Zhong
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  • Ministry of Education Key Laboratory for Ecology of Tropical Islands, Hainan Normal University, Haikou 571158, China

Received date: 2020-10-30

  Online published: 2021-05-14

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摘要

本试验旨在探讨γ-氨基丁酸（GABA）对热应激雏鸡胰腺组织结构、抗氧化能力、消化酶活性及细胞凋亡的影响。将240只1日龄健康雄性文昌鸡雏鸡随机分为4组，分别为对照组（CK组）、热应激组（HS组）、对照+GABA组（CK+G组）和热应激+GABA组（HS+G组），每组6个重复，每个重复10只，常规饲养6周。从2周龄开始，CK组与HS组每日每只灌喂0.2 mL的生理盐水，CK+G组和HS+G组每日每只灌喂0.2 mL GABA水溶液（GABA灌喂量为50 mg/kg BW）；每天12：00—14：00对HS组和HS+G组雏鸡进行（40.0±0.5）℃、相对湿度（70±5）%的热应激。结果显示：GABA可以减轻热应激对雏鸡胰腺造成的损伤，使雏鸡胰腺组织结构保持完整性。在5、6周龄时，CK+G组和HS+G组胰腺组织中超氧化物歧化酶（SOD）活性高于CK组和HS组（P>0.05）；在6周龄时，CK+G组和HS+G组胰腺组织中丙二醛（MDA）含量显著低于HS组（P<0.05）。在3周龄时，HS+G组胰腺组织中脂肪酶活性显著高于CK组（P<0.05）；在4周龄时，CK+G组和HS+G组胰腺组织中胰蛋白酶活性急剧增加，且CK+G组显著高于HS组（P<0.05）。在2、3、4周龄时，CK+G组胰腺组织中淀粉酶mRNA相对表达量显著高于其他3组（P<0.05）；在3周龄时，HS+G组胰腺组织中脂肪酶mRNA相对表达量显著高于CK组（P<0.05），胰蛋白酶mRNA相对表达量显著高于HS组（P<0.05）。在5、6周龄时，HS组凋亡细胞分布密度显著高于其他3组（P<0.05）；在4、6周龄时，HS+G组凋亡细胞分布密度下降，且与CK组差异不显著（P>0.05）。由上述结果可知，添加GABA有助于缓解热应激雏鸡胰腺的损伤，提高雏鸡胰腺组织中消化酶活性，增强抗氧化能力，减少细胞凋亡。

关键词： 雏鸡; 热应激; 胰腺; γ-氨基丁酸; 细胞凋亡

本文引用格式

温静 , 余哲琪 , 田佳迎 , 陈忠 . γ-氨基丁酸对热应激雏鸡胰腺组织结构、抗氧化能力、消化酶活性及细胞凋亡的影响[J]. 动物营养学报, 2021 , 33(5) : 2927 -2938 . DOI: 10.3969/j.issn.1006-267x.2021.05.050

Abstract

The purpose of this experiment was to investigate the effects of γ-aminobutyric acid (GABA) on pancreatic tissue structure, antioxidant capacity, digestive enzyme activities and cell apoptosis of heat-stressed chicks. Two hundred and forty 1-day-old healthy male Wenchang chicken chicks were randomly divided into 4 groups with 6 replicates per group and 10 chicks per replicate, namely control group (CK group), heat stress group (HS group), control+GABA group (CK+G group) and heat stress+GABA group (HS+G group), and regular feeding for 6 weeks. Starting from 2 weeks of age, each chick in the CK group and HS group was given 0.2 mL of normal saline per day, and each chick in the CK+G group and HS+G group was given 0.2 mL of GABA aqueous solution per day (GABA perfusion was 50 mg/kg BW); chicks in the HS group and HS+G group were given heat stress [(40.0±0.5) ℃, relative humidity (70±5)%] every day 12:00 to 14:00. The results showed that GABA could reduce the damage of heat stress to the pancreas of the chicks, and keep the chick’s pancreatic tissue structure intact. At 5 and 6 weeks of age, the activity of superoxide dismutase (SOD) in pancreatic tissue in the CK+G group and HS+G group was higher than that in the CK group and HS group (P>0.05); at 6 weeks of age, the content of malondialdehyde (MDA) in pancreatic tissue in the CK+G group and HS+G group was significantly lower than that in the HS group (P<0.05). At 3 weeks of age, the lipase activity in pancreatic tissue in the HS+G group was significantly higher than that in the CK group (P<0.05); at 4 weeks of age, the trypsin activity in pancreatic tissue in the CK+G group and HS+G group increased sharply, and it in the CK+G group was significantly higher than that in the HS group (P<0.05). At 2, 3 and 4 weeks of age, the relative expression level of amylase mRNA in pancreatic tissue in the CK+G group was significantly higher than that in the other 3 groups (P<0.05); at 3 weeks of age, the relative expression level of lipase in pancreatic tissue in the HS+G group was significantly higher than that in the CK group (P<0.05), and the relative expression level of trypsin mRNA was significantly higher than that of the HS group (P<0.05). At 5 and 6 weeks of age, the distribution density of apoptotic cells in the HS group was significantly higher than that of the other 3 groups (P<0.05); at 4 and 6 weeks of age, the distribution density of apoptotic cells in the HS+G group decreased, and the difference was not significant compared with the CK group (P>0.05). From the above results, it can be seen that the addition of GABA can help alleviate the damage of the pancreas of heat-stressed chicks, increase the activities of digestive enzymes in the pancreatic tissue of the chicks, enhance the antioxidant capacity, and reduce cell apoptosis.

Key words： chicks; heat stress; pancreas; γ-aminobutyric acid; cell apoptosis

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