动物营养学报 >

2021 , Vol. 33 >Issue 6: 3218 - 3227

DOI: https://doi.org/10.3969/j.issn.1006-267x.2021.06.024

禽营养与饲料 POULTRY NUTRITION AND FEED

表皮生长因子对肉鸡十二指肠形态结构、消化酶活性以及表皮生长因子受体表达的影响

  • 单春乔 ,
  • 刘秋晨 ,
  • 李娟 ,
  • 刘恩 ,
  • 陆继爽 ,
  • 刘艳
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  • 1. 大连三仪生物工程研究所, 大连 116036;
    2. 江苏三仪科研质量控制中心, 邳州 221300
单春乔(1983-),女,辽宁大连人,副教授,硕士,从事动物营养与微生态制剂研究。E-mail:88617101@qq.com

收稿日期: 2020-12-13

  网络出版日期: 2021-06-10

基金资助

辽宁省"兴辽英才计划"项目科技创业领军人才（XLYC1803014）

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Effects of Epidermal Growth Factor on Duodenal Morphology, Digestive Enzyme Activities and Epidermal Growth Factor Receptor Expression of Broilers

  • SHAN Chunqiao ,
  • LIU Qiuchen ,
  • LI Juan ,
  • LIU En ,
  • LU Jishuang ,
  • LIU Yan
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  • 1. Dalian Sanyi Bioengineering Research Institute, Dalian 116036, China;
    2. Jiangsu Sanyi Scientific Research Quality Control Center, Pizhou 221300, China

Received date: 2020-12-13

  Online published: 2021-06-10

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摘要

本试验旨在研究肉鸡饲粮中添加表皮生长因子（EGF）对十二指肠形态结构、消化酶活性和表皮生长因子受体（EGFR）表达的影响。试验选用72只1日龄爱拔益加（AA）肉鸡，随机分成4个组，每组3个重复，每个重复6只。对照组（CK组）饲喂基础饲粮，抗生素组（K组）在基础饲粮中添加250 mg/kg盐酸金霉素，EGF组（E组）在基础饲粮中添加500 mg/kg EGF，EGF+抗生素组（E+K组）在基础饲粮中添加500 mg/kg EGF+250 mg/kg盐酸金霉素。试验期为42 d。采用苏木精-伊红（HE）染色、双抗体夹心和原位杂交方法，分别检测了十二指肠黏膜形态，碱性蛋白酶、脂肪酶和α-淀粉酶活性以及EGFR的表达。结果表明：1）28、35、42日龄时，各组十二指肠碱性蛋白酶活性无显著差异（P>0.05），但E组十二指肠碱性蛋白酶活性均为最高。21、28、35、42日龄时，各组十二指肠脂肪酶活性无显著差异（P>0.05），但E组十二指肠脂肪酶活性均为最高。35日龄时，E组十二指肠α-淀粉酶活性显著高于CK组和E+K组（P<0.05）；42日龄时，E组十二指肠α-淀粉酶活性极显著高于K组和E+K组（P<0.01）。2）21日龄时，E组十二指肠EGFR基因平均光密度值显著高于CK组（P<0.05）；42日龄时，E组和K组十二指肠EGFR基因平均光密度值极显著高于CK组和E+K组（P<0.01）。3）21日龄时，K组和E组十二指肠绒毛高度和绒毛高度/隐窝深度显著高于CK组（P<0.05）。28、35和42日龄时，各组十二指肠绒毛高度、隐窝深度和绒毛高度/隐窝深度无显著差异（P>0.05）。21、35和42日龄时，E组绒毛高度/隐窝深度均为最高。综上所述，饲粮添加500 mg/kg EGF能促进21、28、35、42日龄肉鸡十二指肠黏膜形态的发育，增强十二指肠中碱性蛋白酶、脂肪酶和α-淀粉酶活性，提高十二指肠EGFR的表达。

关键词： 表皮生长因子; 肉鸡; 十二指肠; 形态结构; 消化酶活性

本文引用格式

单春乔 , 刘秋晨 , 李娟 , 刘恩 , 陆继爽 , 刘艳 . 表皮生长因子对肉鸡十二指肠形态结构、消化酶活性以及表皮生长因子受体表达的影响[J]. 动物营养学报, 2021 , 33(6) : 3218 -3227 . DOI: 10.3969/j.issn.1006-267x.2021.06.024

Abstract

The purpose of this experiment was to study the effects of dietary epidermal growth factor (EGF) on duodenal morphology, digestive enzyme activities and epidermal growth factor receptor (EGFR) expression of broilers. Seventy-two 1-day-old Arbor Acres (AA) broilers were randomly divided into 4 groups with 3 replicates per group and 6 chickens per replicate. The control group (group CK) was fed a basal diet, the antibiotic group (group K) was fed the basal diet supplemented with 250 mg/kg chlortetracycline hydrochloride, the EGF group (group E) was fed the basal diet supplemented with 500 mg/kg EGF, and the EGF+antibiotic group (group E+K) was fed the basal diet supplemented with 500 mg/kg EGF+250 mg/kg chlortetracycline hydrochloride. The test period was 42 days. The hematoxylin-eosin (HE) staining, double antibody sandwich and in situ hybridization methods were used to detect the duodenal mucosa morphology, the activities of alkaline protease, lipase and α-amylase and the expression of EGFR. The results showed as follows:1) at 28, 35 and 42 days of age, there was no significant difference in duodenal alkaline protease activity among all group (P>0.05), but the duodenal alkaline protease activity in group E was the highest. At 21, 28, 35 and 42 days of age, there was no significant difference in duodenal lipase activity among all group (P>0.05), but the duodenal lipase activity in group E was the highest. At 35 days of age, the duodenal α-amylase activity in group E was significantly higher than that in group CK and group E+K (P<0.05); at 42 days of age, the duodenal α-amylase activity in group E was significantly higher than that in group K and group E+K (P<0.01). 2) At 21 days of age, the duodenal EGFR gene average optical density in group E was significantly higher than that of group CK (P<0.05); at 42 days of age, the duodenal EGFR gene average optical density in group E and group K was significantly higher than that in group CK and group E+K (P<0.01). 3) At 21 days of age, the duodenal villi height and villus height/crypt depth in group K and group E were significantly higher than those in group CK (P<0.05). At 28, 35 and 42 days of age, there were no significant differences in the duodenal villi height, crypt depth, and villus height/crypt depth among all groups (P>0.05). At 21, 35 and 42 days of age, the villus height/crypt depth in group E was the highest. In summary, diet adding 500 mg/kg EGF can promote the development of the duodenal mucosa morphology of broilers at 21, 28, 35 and 42 days of age, and enhance the activities of alkaline protease, lipase and α-starch in duodenum, improve the duodenal EGFR expression.

Key words： epidermal growth factor; broilers; duodenum; morphology; digestive enzyme activities

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