动物营养学报 >

2021 , Vol. 33 >Issue 6: 3309 - 3322

DOI: https://doi.org/10.3969/j.issn.1006-267x.2021.06.033

反刍与草食动物营养与饲料 RUMINANT AND HERBIVORE NUTRITION AND FEED

肝脏转录组分析揭示金银花提取物缓解奶牛热应激的分子机制

  • 高铎 ,
  • 马峰涛 ,
  • 单强 ,
  • 金宇航 ,
  • 李洪洋 ,
  • 常美楠 ,
  • 孙鹏
展开
  • 中国农业科学院北京畜牧兽医研究所, 动物营养学国家重点实验室, 北京 100193
高铎(1994-),男,山东滕州人,硕士研究生,从事动物营养与饲料科学研究。E-mail:694644596@qq.com

收稿日期: 2020-11-10

  网络出版日期: 2021-06-10

基金资助

国家重点研发计划（2016YFD0500507）；中国农业科学院科技创新工程（ASTIP-IAS07）

收起

Hepatic Transcriptome Analyses Revealed Molecular Mechanism of Lonicera japonica Extract in Relieving Heat Stress of Dairy Cows

  • GAO Duo ,
  • MA Fengtao ,
  • SHAN Qiang ,
  • JIN Yuhang ,
  • LI Hongyang ,
  • CHANG Meinan ,
  • SUN Peng
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  • State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China

Received date: 2020-11-10

  Online published: 2021-06-10

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摘要

本试验旨在研究金银花提取物对热应激奶牛生产性能、抗氧化性能、内分泌和免疫功能以及肝脏转录组的影响，以揭示金银花提取物缓解奶牛热应激的分子机制。试验选取胎次、产奶量和泌乳日龄相近的泌乳中期荷斯坦奶牛6头，随机分为2组，每组3头。对照组（CON组）饲喂基础饲粮，不添加金银花提取物；金银花提取物组（LJE组）在基础饲粮中添加28 g/d金银花提取物。预试期2周，正试期8周。试验期间，奶牛所处环境的平均温湿度指数（THI）>72。结果表明，1）与CON组相比，LJE组热应激奶牛直肠温度显著降低（P<0.05）。2）2组之间的干物质采食量、产奶量和乳成分无显著差异（P>0.05）。3）LJE组血清白细胞介素-4、免疫球蛋白G、葡萄糖、胰高血糖素含量和谷胱甘肽过氧化物酶活性以及总抗氧化能力显著高于CON组（P<0.05）。4）肝脏转录组分析发现，CON组和LJE组之间共有17 668个差异表达基因。当差异倍数（FC）>1.200或<0.667且P<0.05时，CON组和LJE组之间共发现253个差异表达基因。基因本体论（GO）和京都基因与基因组百科全书（KEGG）富集分析显示，差异表达基因主要参与免疫反应、炎症反应、泛酸代谢、碳水化合物代谢、质膜和细胞黏附过程。综上所述，饲粮中添加金银花提取物在不影响泌乳奶牛生产性能的情况下，能够缓解奶牛热应激。并且，金银花提取物可以引起肝脏中免疫、抗氧化能力、肝脏葡萄糖代谢等相关基因表达的变化。

关键词： 奶牛; 热应激; 肝脏; 金银花提取物; 转录组

本文引用格式

高铎 , 马峰涛 , 单强 , 金宇航 , 李洪洋 , 常美楠 , 孙鹏 . 肝脏转录组分析揭示金银花提取物缓解奶牛热应激的分子机制[J]. 动物营养学报, 2021 , 33(6) : 3309 -3322 . DOI: 10.3969/j.issn.1006-267x.2021.06.033

Abstract

This study was conducted to investigate the effect of Lonicera japonica extract on performance, antioxidant ability, endocrine and immune function and the hepatic transcriptome of heat-stressed dairy cows, and to reveale molecular mechanism of Lonicera japonica extract in relieving heat stress of dairy cows. Six Holstein cows in mid lactation with similar parity, milk yield and lactation day were randomly divided into 2 groups with 3 cows per group. Cows in the control group (CON group) were fed a basal diet without Lonicera japonica extract, and cows in Lonicera japonica extract group (LJE group) were fed a basal diet supplemented with 28 g/d Lonicera japonica extract. The pre-experimental period lasted for 2 weeks, and the experimental period lasted for 8 weeks. During the experimental period, the average temperature and humidity index (THI) of environment of cows was higher than 72. The results showed as follows:1) compared with the CON group, the rectal temperature of heat-stressed dairy cows of LJE group was significantly decreased (P<0.05). 2) There were no significant differences in dry matter intake, milk yield and milk composition between two groups (P>0.05). 3) The interleukin-4, immunoglobulin G, glucose and glucagon contents and glutathione peroxidase activity and total antioxidant capacity in serum of LJE group were significantly higher than those of the CON group (P<0.05). 4) The hepatic transcriptome analysis found a total of 17 668 differentially expressed genes between the CON group and LJE group. A total of 253 differentially expressed genes were found between the CON group and LJE group after taking into account the criteria fold change (FC) >1.200 or <0.667 and P<0.05. Gene ontology (GO) and Kyoto encyclopedia of genes and genomes (KEGG) enrichment analysis revealed that differentially expressed genes were mainly involved in immune, inflammatory, pantothenate metabolic, carbohydrate metabolic processes, plasma membrane and cell adhesion. Collectively, dietary supplemented with Lonicera japonica extract can relieve heat stress of dairy cows without affect the performance of lactating cows. In addition, Lonicera japonica extract can cause changes of hepatic gene expression, such as immune, antioxidant capacity and liver glucose metabolism related genes.

Key words： dairy cows; heat stress; liver; Lonicera japonica extract; transcriptome

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