实验方法与实验动物 EXPERIMENTAL METHOD AND ANIMAL

富硒酵母对长期缺硒和正常大鼠体内D-丝氨酸和L-丝氨酸水平的影响

  • 余海立 ,
  • 张彦 ,
  • 张凤伟 ,
  • 张海波 ,
  • 程倩 ,
  • 陈智仙 ,
  • 董运海 ,
  • 李婉奕 ,
  • 张双庆
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  • 1. 安琪酵母股份有限公司, 酵母功能湖北省重点实验室, 宜昌 443003;
    2. 中国疾病预防控制中心营养与健康所, 北京 100050
余海立(1992-),男,安徽六安人,硕士,研究方向为营养与保健。E-mail:yuhl@angelyeast.com

收稿日期: 2020-11-13

  网络出版日期: 2021-06-10

基金资助

国家自然科学基金项目(82073855);湖北省自然科学基金项目(2018CFB612)

Effects of Selenium-Enriched Yeast on D-Serine and L-Serine Levels in Long-Term Selenium-Deficient and Normal Rats

  • YU Haili ,
  • ZHANG Yan ,
  • ZHANG Fengwei ,
  • ZHANG Haibo ,
  • CHENG Qian ,
  • CHEN Zhixian ,
  • DONG Yunhai ,
  • LI Wanyi ,
  • ZHANG Shuangqing
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  • 1. Hubei Provincial Key Laboratory of Yeast Function, Angel Yeast Co., Ltd., Yichang 443003, China;
    2. National Institute for Nutrition and Health, Chinese Center for Disease Control and Prevention, Beijing 100050, China

Received date: 2020-11-13

  Online published: 2021-06-10

摘要

本试验在建立缺硒大鼠模型的基础上,研究饲粮补充富硒酵母对长期缺硒和正常大鼠体内D-丝氨酸与L-丝氨酸水平的影响。将3周龄Sprague-Dawley大鼠随机分为缺硒组和正常组,分别饲喂低硒饲粮(含硒0.02 mg/kg)和正常饲粮(含硒0.18 mg/kg),饲喂期为420 d。第421天,将缺硒组大鼠分为4组:缺硒空白对照组(SD-B组,n=17)、缺硒富硒酵母低剂量组(SD-LY组,n=16)、缺硒富硒酵母中剂量组(SD-MY组,n=20)、缺硒富硒酵母高剂量组(SD-HY组,n=16)。将正常组大鼠分为2组:正常空白对照组(SS-B组,n=20)、正常富硒酵母中剂量组(SS-MY组,n=20)。SD-B组继续饲喂低硒饲粮,SD-LY、SD-MY、SD-HY组分别饲喂含0.18、0.36和0.72 mg/kg硒的富硒酵母饲粮,SS-B组继续饲喂正常饲粮,SS-MY组饲喂含0.36 mg/kg硒的富硒酵母饲粮,饲喂期为112 d。结果显示:1)缺硒60 d后,缺硒组大鼠体重显著低于正常组大鼠(P<0.05)。2)补硒112 d后,与SD-B组相比,SD-LY、SD-MY、SD-HY、SS-B、SS-MY组血浆硒水平显著升高(P<0.05);SD-LY、SD-MY、SD-HY、SS-MY组大脑皮质硒水平显著升高(P<0.05);SD-MY、SD-HY、SS-MY组小脑硒水平显著升高(P<0.05)。与SS-B组相比,SS-MY组小脑和大脑皮质硒水平显著升高(P<0.05),血浆硒水平无显著变化(P>0.05)。3)补硒112 d后,与SD-B组相比,SD-LY、SD-MY、SD-HY、SS-B和SS-MY组血浆L-丝氨酸水平显著降低(P<0.05),D-丝氨酸水平无显著变化(P>0.05);SD-LY组大脑皮质L-丝氨酸水平显著降低(P<0.05),SD-LY、SD-MY、SD-HY、SS-B和SS-MY组大脑皮质D-丝氨酸水平无显著变化(P>0.05);SD-HY组海马L-丝氨酸和D-丝氨酸水平极显著升高(P<0.01),SD-LY、SD-MY、SS-B和SS-MY组海马L-丝氨酸和D-丝氨酸水平无显著变化(P<0.05)。与SS-B组相比,SS-MY组血浆、大脑皮质和海马L-丝氨酸、D-丝氨酸水平无显著变化(P>0.05)。由此得出,饲粮补充富硒酵母显著升高大鼠长期缺硒引起的血浆硒水平下降,增加大脑皮质与小脑硒水平,降低血浆L-丝氨酸水平,升高海马L-丝氨酸与D-丝氨酸水平。此外,饲粮补充富硒酵母还可显著升高正常大鼠大脑皮质与小脑硒水平。

本文引用格式

余海立 , 张彦 , 张凤伟 , 张海波 , 程倩 , 陈智仙 , 董运海 , 李婉奕 , 张双庆 . 富硒酵母对长期缺硒和正常大鼠体内D-丝氨酸和L-丝氨酸水平的影响[J]. 动物营养学报, 2021 , 33(6) : 3515 -3522 . DOI: 10.3969/j.issn.1006-267x.2021.06.053

Abstract

This experiment was conducted to establish a selenium (Se)-deficient rat model and investigate the effects of dietary supplemented with Se-enriched yeast (SeY) on D-serine and L-serine levels in long-term Se-deficient and normal rats. Three-week-old Sprague-Dawley rats were randomly divided into two groups. Rats in Se-deficient group were fed a low Se diet (0.02 mg/kg Se) and those in normal group were fed a normal diet (0.18 mg/kg Se) for 420 days. On day 421, Se-deficient rats were further divided into four groups:Se-deficient blank group (SD-B group, n=17), Se-deficient low-dose SeY-treated group (SD-LY group, n=16), Se-deficient middle-dose SeY-treated group (SD-MY group, n=20) and Se-deficient high-dose SeY-treated group (SD-HY, n=16); normal rats were further divided into two groups:normal blank group (SS-B group, n=20) and normal middle-dose SeY-treated group (SS-MY group, n=20). Rats in SD-B group still received the Se-deficient diet for 112 days. Rats in SD-LY, SD-MY and SD-HY groups were fed SeY diets with 0.18, 0.36 and 0.72 mg/kg Se for 112 days, respectively. Rats in SS-B group still received the normal diet for 112 days. Rats in SS-MY group were fed the SeY diet with 0.36 mg/kg Se for 112 days. The results showed as follows:1) after 60 days of Se-deficient, the body weight of rats in the Se-deficient group was significantly lower than that in the normal group (P<0.05). 2) After 112 days of supplementing Se, compared with the SD-B group, plasma Se level of rats in SD-LY, SD-MY, SD-HY, SS-B and SS-MY groups was significantly increased (P<0.05), and cerebral cortex Se level in SD-LY, SD-MY, SD-HY and SS-MY groups was significantly elevated (P<0.05), and cerebellum Se level in SD-MY, SD-HY and SS-MY groups was significantly enhanced (P<0.05). Compared with the SS-B group, cerebral cortex and cerebellum Se level of rats in SS-MY group were significantly elevated (P<0.05), but plasma Se level had no significant change (P>0.05). 3) After 112 days of supplementing Se, compared with the SD-B group, plasma L-serine level of rats in SD-LY, SD-MY, SD-HY, SS-B and SS-MY groups was significantly decreased (P<0.05), and but plasma D-serine level had no significant change (P>0.05); cerebral cortex L-serine level in SD-LY group was significantly decreased (P<0.05), and cerebral cortex D-serine level of SD-LY, SD-MY, SD-HY, SS-B and SS-MY groups had no significant change (P>0.05); hippocampus L-serine and D-serine levels in SD-HY groups were extremely significantly increased (P<0.01), and hippocampus L-serine and D-serine levels in SD-LY, SD-MY, SS-B and SS-MY groups had no significant change (P>0.05). Compared with the SS-B group, plasma, cerebral cortex and hippocampus L-serine and D-serine levels of rats in SS-MY group had no significant change (P>0.05). In conclusion, supplementing SeY in the diet can significantly increase plasma Se level of long-term selenium-deficient rats, increase cerebral cortex and cerebellum Se level, decrease plasma L-serine level, and increase plasma L-serine and D-serine levels. Furthermore, supplementing SeY in the diet also can significantly increase the Se level in cerebral cortex and cerebellum of normal rats.

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