研究简报 SHORT COMMUNICATION

饲粮中锌添加水平对生长獭兔毛囊发育及相关基因表达的影响

  • 赵曼 ,
  • 杨国雨 ,
  • 刘永需 ,
  • 刘磊 ,
  • 李福昌
展开
  • 1. 山东农业大学动物科技学院, 山东省动物生物工程与疾病防治重点实验室, 泰安 271018;
    2. 青岛康大食品有限公司, 青岛 266555
赵曼(1998-),女,甘肃东乡族人,硕士研究生,从事家兔营养与代谢研究。E-mail:1020371479@qq.com

收稿日期: 2020-12-31

  网络出版日期: 2021-06-10

基金资助

现代农业产业技术体系建设专项(CARS-43-B-1);山东省博士后创新项目(2020年);新疆自治区重点研发计划项目(2020B01004-1-3);泰山产业领军人才项目(TSCY20190107)

Effects of Dietary Zinc Supplemental Level on Hair Follicle Development and Related Gene Expression of Growing Rex Rabbits

  • ZHAO Man ,
  • YANG Guoyu ,
  • LIU Yongxu ,
  • LIU Lei ,
  • LI Fuchang
Expand
  • 1. Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Animal Science and Technology, Shandong Agricultural University, Tai'an 271018, China;
    2. Qingdao Kangda Food Co., Ltd., Qingdao 266555, China

Received date: 2020-12-31

  Online published: 2021-06-10

摘要

本试验旨在研究饲粮中锌添加水平对生长獭兔毛囊发育及相关基因表达的影响。选择160只初始体重相近的3月龄健康獭兔,随机分为4组,每组40个重复,每个重复1只兔。4组獭兔分别饲喂在基础饲粮中添加0(对照组)、40、80和120 mg/kg锌(以硫酸锌的形式)的饲粮。预试期7 d,正试期49 d。结果显示:与对照组相比,饲粮中添加40和80 mg/kg锌时獭兔的毛囊密度显著升高(P<0.05),饲粮中添加120 mg/kg锌对獭兔的毛囊密度无显著影响(P>0.05)。与对照组相比,饲粮中添加40和80 mg/kg锌显著提高了獭兔神经诱导蛋白(Noggin)基因的相对表达量(P<0.05),显著降低了骨形态发生蛋白4(BMP4)基因的相对表达量(P<0.05),对胞外基质蛋白多糖(Versican)、碱性磷酸酶(ALP)、肝细胞生长因子(HGF)、骨形态发生蛋白2(BMP2)和胰岛素样生长因子-1(IGF-1)基因的相对表达量没有显著影响(P>0.05)。综上所述,以硫酸锌形式在3~5月龄生长獭兔饲粮中添加40或80 mg/kg锌时,可通过上调Noggin基因的表达,下调BMP4基因的表达,减少对毛囊生长的抑制并激活毛囊上皮细胞增殖,从而促进毛囊的形成和发育。

本文引用格式

赵曼 , 杨国雨 , 刘永需 , 刘磊 , 李福昌 . 饲粮中锌添加水平对生长獭兔毛囊发育及相关基因表达的影响[J]. 动物营养学报, 2021 , 33(6) : 3532 -3541 . DOI: 10.3969/j.issn.1006-267x.2021.06.055

Abstract

The aim of this study was to investigate the effects of dietary zinc supplemental level on hair follicle development and related gene expression of growing Rex rabbits. One hundred and sixty 3-month-old Rex rabbits with similar body weight were selected and randomly divided into 4 groups with 40 replicates in each group and 1 rabbit in each replicate. Rex rabbits in the 4 groups were fed diets supplemented with 0 (control group), 40, 80 and 120 mg/kg zinc (in the form of zinc sulfate), respectively. The pre-experimental period lasted for 7 days, and the experimental period lasted for 49 days. The results showed as follows:compared with the control group, diets supplemented with 40 and 80 mg/kg zinc could significantly increase the hair follicle density of Rex rabbits (P<0.05), but diets supplemented with 120 mg/kg zinc had no significant influence on the hair follicle density (P>0.05). Compared with the control group, diets supplemented with 40 and 80 mg/kg zinc could significantly increase the relative expression level of noggin protein (Noggin) gene of Rex rabbits (P<0.05), but significantly decrease the relative expression level of bone morphogenetic protein 4 (BMP4) gene (P<0.05). The relative expression levels of extracellular matrix proteoglycans (Versican), alkaline phosphatase (ALP), hepatocyte growth factor (HGF), bone morphogenetic protein 2 (BMP2) and insulin-like growth factor-1 (IGF-1) genes were not significantly affected by dietary zinc supplemental level (P>0.05). In summary, adding 40 or 80 mg/kg zinc in the form of zinc sulfate to the diet of 3- to 5-month-old growing Rex rabbits can promote the formation and development of hair follicles by up-regulating the expression of Noggin gene and down-regulating the expression of BMP4 gene, reduce the inhibitory effect on hair follicle growth and activate the proliferation of hair follicle epithelial cells, and then promote the formation and development of hair follicle.

参考文献

[1] 杜敏,杨玉艾,张晓敏,等.微量元素锌的研究进展[J].黑龙江畜牧兽医,2014(9):61-63. DU M,YANG Y A,ZHANG X M,et al.Research progress of trace element zinc[J].Heilongjiang Animal Husbandry and Veterinary Medicine,2014(9):61-63.(in Chinese)
[2] ADEBAYO O L,ADENUGA G A,SANDHIR R.Selenium and zinc protect brain mitochondrial antioxidants and electron transport chain enzymes following postnatal protein malnutrition[J].Life Sciences,2016,152:145-155.
[3] 郝丽媛,马峰涛,孙鹏.饲粮添加蛋氨酸锌或氧化锌对新生犊牛锌代谢的影响[J].动物营养学报,2020,32(8):3725-3731. HAO L Y,MA F T,SUN P.Effects of dietary zinc methionine or zinc oxide on zinc metabolism in neonatal calves[J].Chinese Journal of Animal Nutrition,2020,32(8):3725-3731.(in Chinese)
[4] 左楠.微量元素锌对机体免疫功能的影响研究进展[J].兽医导刊,2016(4):215. ZUO N.Research progress on the effect of trace element zinc on immune function[J].Veterinary Orientation,2016(4):215.(in Chinese)
[5] ATTIA Y A,BOVERA F,ABD EI-HAMID A E,et al.Effect of zinc bacitracin and phytase on growth performance,nutrient digestibility,carcass and meat traits of broilers[J].Journal of Animal Physiology and Animal Nutrition,2016,100(3):485-491.  
[6] 白玉恒,王荣斌,刘锦旺,等.饲粮锌水平对生绒期陕北白绒山羊生长性能、产绒性能及绒生长相关激素含量的影响[J].动物营养学报,2018,30(6):2202-2208. BAI Y H,WANG R B,LIU J W,et al.Effects of dietary zinc level on growth performance,production performance and growth-related hormone content of white cashmere goats in northern Shaanxi during the cashmere growing period[J].Chinese Journal of Animal Nutrition,2018,30(6):2202-2208.(in Chinese)
[7] 郭强.饲粮锌水平和锌源对蓝狐生产性能的影响[D].硕士学位论文.北京:中国农业科学院,2014. GUO Q.Effects of dietary zinc levels and zinc sources on the production performance of blue foxes[D].Master's Thesis.Beijing:Chinese Academy of Agricultural Sciences,2014.(in Chinese)
[8] LIUZZI J P,BLANCHARD R K,COUSINS R J.Differential regulation of zinc transporter 1,2,and 4 mRNA expression by dietary zinc in rats[J].The Journal of Nutrition,2001,131(1):46-52.  
[9] KRAMETTER-FROETSCHER R,HAUSER S,BAUMGARTNER W.Zinc-responsive dermatosis in goats suggestive of hereditary malabsorption:two field cases[J].Veterinary Dermatology,2005,16(4):269-275.  
[10] 杨国雨,刘公言,陈晓阳,等.饲粮锌添加水平对生长獭兔生长性能、屠宰性能、肌肉品质、毛皮质量及氮代谢的影响[J].动物营养学报,2019,31(4):1936-1942. YANG G Y,LIU G Y,CHEN X Y,et al.Add fodder zinc levels on the growth of rabbit fur growth performance,slaughter performance and meat quality,the quality and the effect of nitrogen metabolism[J].Chinese Journal of Animal Nutrition,2019,31(4):1936-1942.(in Chinese)
[11] NRC.Nutrient requirements of rabbits[S].2nd ed.Washington,D.C.:National Academy Press,1977.
[12] 中国饲料数据库.中国饲料成分及营养价值表(2007年第18版)[J].中国饲料,2007(21):34-39. CFDB.Tables of feed composition and nutritive values in China (18th edition,2007)[J].China Feed,2007(21):34-39.(in Chinese)
[13] 李红芬,郑肇巽,马品耀,等.HE染色原理和试剂配制及染色过程中的若干问题的探讨[J].医学信息,2011,24(4):1985-1986. LI H F,ZHENG Z X,MA P Y,et al.HE dyeing principle and reagent preparation and the discussion of some problems in the process of[J].Journal of Medical Information,2011,24(4):1985-1986.(in Chinese)
[14] 范康,傅祥超,范成强,等.獭兔毛囊分化与被毛生长规律研究[J].中国养兔,2011(12):6-9. FAN K,FU X C,FAN C Q,et al.Differentiation of rabbit hair follicles and coat growth rule research[J].Chinese Journal of Rabbit Farming,2011(12):6-9.(in Chinese)
[15] 王丹丹,肖向红,吴立舒,等.改良TRIzol法高效提取东北林蛙皮肤总RNA[J].野生动物,2012,33(3):127-128,138. WANG D D,XIAO X H,WU L S,et al.The modified Trizol method was used to efficiently extract total RNA from the skin of Rana chinensis[J].Journal of Animal Science,2012,33(3):127-128,138.(in Chinese)
[16] COUSINS R J.A role of zinc in the regulation of gene expression[J].Proceedings of the Nutrition Society,1998,57(2):307-311.  
[17] 王惠影,刘毅,雷胡龙,等.饲粮锌水平对鹅再生羽绒品质、血清指标及毛囊发育相关基因表达的影响[J].动物营养学报,2020,32(4):1616-1623. WANG H Y,LIU Y,LEI H L,et al.Effects of dietary zinc level on quality,serum index and hair follicle-developing-related gene expression in geese[J].Chinese Journal of Animal Nutrition,2020,32(4):1616-1623.(in Chinese)
[18] 闫海亮.日粮蛋氨酸锌对獭兔生产性能、生化指标的影响[D].硕士学位论文.长春:吉林大学,2008. YAN H L.Effects of dietary zinc methionine on performance and biochemical indices of Rex rabbits[D].Master's Thesis.Changchun:Jilin University,2008.(in Chinese)
[19] SALAMA A A K,CAJA G,ALBANELL E,et al.Effects of dietary supplements of zinc-methionine on milk production,udder health and zinc metabolism in dairy goats[J].Journal of Dairy Research,2003,70(1):9-17.  
[20] 刘海英,杨桂芹,李学俭,等.不同日粮锌水平对辽宁绒山羊公羊绒毛生长,血浆睾酮和锌含量的影响[C]//2015年全国养羊生产与学术研讨会论文集.登封:中国畜牧兽医学会养羊学分会,2015:1. LIU H Y,YANG G Q,LI X J,et al.Effects of different dietary zinc levels on cashmere hair growth,plasma testosterone and zinc content of Liaoning cashmere ram[C]//Proceedings of 2015 National Sheep Production and Academic Symposium.Dengfeng:Chinese Society of Animal Husbandry and Veterinary Medicine Sheep Science Branch,2015:1.(in Chinese)
[21] WHITE C L,MARTIN G B,HYND P I,et al.The effect of zinc deficiency on wool growth and skin and wool follicle histology of male Merino lambs[J].British Journal of Nutrition,1994,71(3):425-435.  
[22] 耿文静.不同锌水平对银狐生产性能和血液生化指标的影响[D].硕士学位论文.北京:中国农业科学院,2010. GENG W J.Effects of different zinc levels on the production performance and blood biochemical indexes of silver foxes[D].Master's Thesis.Beijing:Chinese Academy of Agricultural Sciences,2010.(in Chinese)
[23] PLONKA P M,HANDJISKI B,POPIK M,et al.Zinc as an ambivalent but potent modulator of murine hair growth in vivo-preliminary observations[J].Experimental Dermatology,2005,14(11):844-853.  
[24] YANG B L,ZHANG Y,CAO L,et al.Cell adhesion and proliferation mediated through the G1 domain of Versican[J].Journal of Cellular Biochemistry,1999,72(2):210-220.  
[25] 刘欣,祝宁侠.蛋白聚糖在毛囊中的研究进展[J].医学理论与实践,2020,33(5):721-723. LIU X,ZHU N X.Research progress of proteoglycan in hair follicles[J].Medical theory and practice,2020,33(5):721-723.(in Chinese)
[26] SOMA T,TAJIMA M,KISHIMOTO J.Hair cycle-specific expression of Versican in human hair follicles[J].Journal of Dermatological Science,2005,39(3):147-154.  
[27] WADSTEIN J,THOM E,GADZHIGOROEVA A.Integral roles of specific proteoglycans in hair growth and hair loss:mechanisms behind the bioactivity of proteoglycan replacement therapy with nourkrin with Marilex in pattern hair loss and telogen effluvium[J].Dermatology Research and Practice,2020,2020:8125081.
[28] 高琴.日粮钴添加水平对生长獭兔生产性能、氮代谢性能和毛囊发育的影响[D].硕士学位论文.泰安:山东农业大学,2017. GAO Q.Effects of dietary cobalt supplementation on production performance,nitrogen metabolism and hair follicle development of growing Rex rabbits[D].Master's Thesis.Tai'an:Shandong Agricultural University,2017.(in Chinese)
[29] 杨翠军,葛剑,吴淑琴.日粮锌水平对生长期獭兔生长性能和血清生化指标的影响[J].中国养兔,2019(2):4-7. YANG C J,GE J,WU S Q.Effects of dietary zinc levels on growth performance and serum biochemical indexes of Rex rabbits in growing period[J].Chinese Journal of Rabbit Farming,2019(2):4-7.(in Chinese)
[30] 苏莉娜,王安.锌对蛋雏鸭生长性能和血液生化指标的影响[J].中国饲料,2012(12):35-37,42. SU L N,WANG A.Effects of zinc on growth performance and blood biochemical indices of egg ducklings[J].China Feed,2012(12):35-37,42.(in Chinese)
[31] 芦春莲,李建国,李艳琴,等.高锌对奶牛血液理化指标的影响[J].黑龙江畜牧兽医,2003(9):11-13. LU C L,LI J G,LI Y Q,et al.Effects of high Zn on blood parameters of cows[J]Heilongjiang Animal Husbandry and Veterinary Medicine,2003(9):11-13.(in Chinese)
[32] 范卫新,朱文元.肝细胞生长因子对小鼠触须毛囊体外培养的研究[J].中华皮肤科杂志,1999,32(5):347-348. FAN W X,ZHU W Y.Studies on hepatocyte growth factor in vitro culture of mouse tentacle hair follicles[J].Chinese Journal of Dermatology,1999,32(5):347-348.(in Chinese)
[33] JINDO T,TSUBOI R,IMAI R,et al.Hepatocyte growth factor/scatter factor stimulates hair growth of mouse vibrissae in organ culture[J].The Journal of Investigative Dermatology,1994,103(3):306-309.  
[34] 于秀菊,高淑媛,程笳琪,等.HGF/c-met在小鼠毛囊生长周期中的表达[J].中国实验动物学报,2015,23(4):406-409. YU X J,GAO S Y,CHENG J Q,et al.Expression of HGF/c-met in mouse hair follicle growth cycle[J].Chinese Journal of Experimental Animals,2015,23(4):406-409.(in Chinese)
[35] STEWART C E,ROTWEIN P.Growth,differentiation,and survival:multiple physiological functions for insulin-like growth factors[J].Physiological Reviews,1996,76(4):1005-1026.  
[36] 俞成浩,郭志强,雷岷,等.乳酸锌对断奶肉兔生长性能、肠道发育和血清生化指标的影响[J].动物营养学报,2021,33(2):1055-1062. YU C,GUO Z Q,LEI M,et al.Effects of zinc lactate on growth performance,intestinal development and serum biochemical indexes in weaned rabbits[J].Chinese Journal of Animal Nutrition,2021,33(2):1055-1062.(in Chinese)
[37] 朱雯,刘建新,叶均安.微量元素锌在奶牛中的应用研究进展[J].中国畜牧杂志,2014,50(15):83-86. ZHU W,LIU J X,YE J A.Trace element zinc application in dairy research progress[J].Chinese Journal of Animal Husbandry,2014,50(15):83-86.(in Chinese)
[38] FREDLUND K,ISAKSSON M,ROSSANDER-HULTHÉN L,et al.Absorption of zinc and retention of calcium:dose-dependent inhibition by phytate[J].Journal of Trace Elements in Medicine and Biology,2006,20(1):49-57.  
[39] FLINIAUX I,MIKKOLA M L,LEFEBVRE S,et al.Identification of dkk4 as a target of Eda-A1/Edar pathway reveals an unexpected role of ectodysplasin as inhibitor of Wnt signalling in ectodermal placodes[J].Developmental Biology,2008,320(1):60-71.  
[40] RISHIKAYSH P,DEV K,DIAZ D,et al.Signaling involved in hair follicle morphogenesis and development[J].International Journal of Molecular Sciences,2014,15(1):1647-1670.  
[41] 李冰,李福昌,朱岩丽,等.不同被毛密度獭兔皮肤差异表达基因的研究[J].畜牧兽医学报,2014,45(10):1631-1639. LI B,LI F C,ZHU Y L,et al.Studies on the different-expressed genes in the skin of Rex rabbits with different coat density[J].Chinese Journal of Animal Husbandry and Veterinary Science,2014,45(10):1631-1639.(in Chinese)
[42] 苏蕊,张文广,尹俊,等.骨形态发生蛋白2(BMP2)基因在内蒙古绒山羊皮肤毛囊发育不同时期的表达[J].华北农学报,2008,23(2):110-113. SU R,ZHANG W G,YIN J,et al.Expression of bone morphogenetic protein 2(BMP2) gene at different stages of Inner Mongolia cashmere goat skin hair follicle development[J].Journal of North China Agronomy,2008,23(2):110-113.(in Chinese)
[43] KULESSA H,TURK G,HOGAN B L.Inhibition of BMP signaling affects growth and differentiation in the anagen hair follicle[J].The EMBO Journal,2000,19(24):6664-6674.  
[44] MILLAR S E.Molecular mechanisms regulating hair follicle development[J].Journal of Investigative Dermatology,2002,118(2):216-225.  
[45] KURODA H,WESSELY O,DE ROBERTIS E M.Neural induction in Xenopus:requirement for ectodermal and endomesodermal signals via chordin,noggin,β-catenin,and cerberus[J].PLoS Biology,2004,2(5):e92.
[46] BOTCHKAREV V A,BOTCHKAREVA N V,ROTH W,et al.Noggin is a mesenchymally derived stimulator of hair-follicle induction[J].Nature Cell Biology,1999,1(3):158-164.  
[47] GUHA U,MECKLENBURG L,COWIN P,et al.Bone morphogenetic protein signaling regulates postnatal hair follicle differentiation and cycling[J].The American Journal of Pathology,2004,165(3):729-740.  
[48] 凌学剑,杨青春,张彦定,等.毛囊真皮鞘细胞中过表达Noggin对皮肤及毛囊生长的作用[J].中国组织工程研究,2020,24(31):5013-5017. LING X J,YANG Q C,ZHANG Y D,et al.Effect of Noggin overexpression in hair follicle dermal sheath cells on the growth of skin and hair follicles[J]Chinese Journal of Tissue Engineering Research,2020,24(31):5013-5017.(in Chinese)
[49] ZIMMERMAN L B,DE JESÚS-ESCOBAR J M,HARLAND R M.The spemann organizer signal noggin binds and inactivates bone morphogenetic protein 4[J].Cell,1996,86(4):599-606.  
[50] 刘公言,白莉雅,李福昌,等.毛囊发育与周期性生长的调控信号通路研究进展[J].畜牧与兽医,2021,53(1):125-129. LIU G Y,BAI L Y,LI F C,et al.Advances in regulating signaling pathway of development and cycling of hair follicles[J]Animal Husbandry and Veterinary Medicine,2021,53(1):125-129.(in Chinese)
[51] BLANPAIN C,FUCHS E.Epidermal stem cells of the skin[J].Annual Review of Cell and Developmental Biology,2006,22:339-373.
[52] 杨梦洁,林蕾,高晶.毛囊发育和循环生长的信号机制的研究进展[J].现代实用医学,2014,26(6):781-784. YANG M J,LIN L,GAO J.Advances in research on signal mechanism of hair follicle development and circulation growth[J].Modern Practical Medicine,2014,26(6):781-784.(in Chinese)
[53] 谈济洲,聂二民,张春元,等.锌离子对炎症微环境下成骨细胞成骨相关蛋白表达的影响[J].中华口腔医学杂志,2016,51(8):486-490. TAN J Z,NIE E M,ZHANG C Y,et al.Effects of zinc ions on osteoblastic protein expression in inflammatory microenvironment of osteoblasts[J].Chinese Journal of Stomatology,2016,51(8):486-490.(in Chinese)
文章导航

/