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桑叶多酚的提取及其体外抗氧化能力测定

  • 冯淦熠 ,
  • 杨浩然 ,
  • 项轩 ,
  • 殷磊 ,
  • 贺喜 ,
  • 曹蓉
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  • 1. 湖南农业大学动物科学技术学院, 湖南畜禽安全生产协同创新中心, 长沙 410128;
    2. 中国科学院亚热带农业生态研究所, 长沙 410125
冯淦熠(1998-),男,湖南湘乡人,硕士研究生,动物营养与饲料科学专业。E-mail:1324658055@qq.com

收稿日期: 2020-11-30

  网络出版日期: 2021-06-10

基金资助

国家重点研发计划政府间国际科技创新合作重点专项(2018YFE0101700);湖南省科技人才托举工程(2020TJ-Q02);湖南省教育厅科学研究项目一般项目(19C0937)

Extraction of Polyphenols from Mulberry Leaves and Its Determination of Antioxidant Capacity in Vitro

  • FENG Ganyi ,
  • YANG Haoran ,
  • XIANG Xuan ,
  • YIN Lei ,
  • HE Xi ,
  • CAO Rong
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  • 1. Hunan Co-Innovation Center of Animal Production Safety, College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China;
    2. The Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China

Received date: 2020-11-30

  Online published: 2021-06-10

摘要

为研究最佳的桑叶多酚提取方法,并综合评价桑叶多酚的体外抗氧化能力,本试验采用浓度分别为60%、70%、80%的乙醇提取桑叶多酚,建立没食子酸标准曲线测定桑叶多酚的绝对量和含量。将各提取组桑叶多酚提取液稀释2、4、8、16倍后测定其1,1-二苯基-2-三硝基苯肼(DPPH)自由基清除率,并以水溶性维生素E(Trolox)为阳性对照,半抑制浓度(IC50)为参考依据,对各组提取液及稀释液进行体外抗氧化能力测定。结果表明:1)60%乙醇提取组的桑叶多酚绝对量和含量最高,但各提取组之间无显著差异(P>0.05)。2)将60%乙醇提取组中的桑叶多酚提取液分别稀释至原液的25%、50%、75%后测定各稀释组的桑叶多酚绝对量及含量,各稀释组之间桑叶多酚绝对量和含量差异极显著(P<0.01)。3)在60%乙醇提取组中,2倍稀释组的DPPH自由基清除率极显著高于其他稀释组(P<0.01);在70%乙醇提取组中,2倍稀释组的DPPH自由基清除率极显著高于16倍稀释组(P<0.01);在80%乙醇提取组中,各稀释水平之间DPPH自由基清除率差异极显著(P<0.01)。4)与Trolox标准品相比,各提取组的IC50均相对较高,其中60%乙醇提取组的IC50极显著低于70%乙醇提取组(P<0.01)。5)桑叶多酚绝对量与IC50呈负相关,但相关关系不显著(P>0.05)。综上所述,桑叶多酚具有良好的体外抗氧化性能,其含量与体外抗氧化能力之间存在一定的量效关系。

本文引用格式

冯淦熠 , 杨浩然 , 项轩 , 殷磊 , 贺喜 , 曹蓉 . 桑叶多酚的提取及其体外抗氧化能力测定[J]. 动物营养学报, 2021 , 33(6) : 3573 -3580 . DOI: 10.3969/j.issn.1006-267x.2021.06.059

Abstract

In order to study the best extraction method of polyphenols from mulberry leaves, and comprehensively evaluate the antioxidant capacity of polyphenols from mulberry leaves, the concentrations 60%, 70% and 80% ethanol were used for extraction of polyphenols from mulberry leaves, respectively, and gallic acid standard curve was established to determine the absolute amount and content of polyphenols from mulberry leaves. The 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging rate in extracting solution of extraction groups was determined after 2, 4, 8 and 16 times dilution, and the antioxidant capacity of extracting solution and diluents in each group was measured in vitro using 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox) as positive control and 50% inhibiting concentration (IC50) as reference. The results showed as follows:1) the absolute amount and content of polyphenols in mulberry leaves of 60% ethanol extraction group were the highest, however, there was no significant difference among the extraction groups (P>0.05). 2) The extracting solution of polyphenols in mulberry leaves of 60% ethanol extraction was diluted to 5%, 50% and 75% of original solution, and the absolute amount and content of polyphenols in mulberry leaves of each dilution group was measured. It was found that the absolute amount and content of polyphenols in mulberry leaves of dilution groups were significantly different (P<0.01). 3) In the 60% ethanol extraction group, the DPPH free radical scavenging rate of polyphenols in mulberry leaves of 2 times dilution group was significantly higher than that of other dilution groups (P<0.01); in the 70% ethanol extraction group, the DPPH free radical scavenging rate of polyphenols in mulberry leaves of 2 times dilution group was significantly higher than that of 16 times dilution group (P<0.01); in the 80% ethanol extract group, the DPPH free radical scavenging rate was significant difference among all dilution groups (P<0.01). 4) Compared with Trolox standard, the IC50 of extract groups was relatively higher, the IC50 of 60% ethanol extract group was significantly lower than that of 70% ethanol extract group (P<0.01). 5) The absolute amount of polyphenols in mulberry leaves was negatively correlated with IC50, however, the correlation was not significant (P>0.05). In conclusion, the polyphenols in mulberry leaves have good antioxidant activity in vitro, and there is a dose-effect relationship between its content and in vitro antioxidant capacity.

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