动物营养学报 >

2021 , Vol. 33 >Issue 6: 3581 - 3591

DOI: https://doi.org/10.3969/j.issn.1006-267x.2021.06.060

研究简报 SHORT COMMUNICATION

不同实验室间测定单胃动物饲料样品总能的一致性及可加性研究

  • 张晋源 ,
  • 王钰明 ,
  • 杜中原 ,
  • 董莹 ,
  • 李黛淋 ,
  • 王跃丽 ,
  • 黄庆华 ,
  • 杜青之 ,
  • 黄艳玲 ,
  • 赵峰
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  • 1. 西南民族大学畜牧兽医学院, 成都 610041;
    2. 中国农业科学院北京畜牧兽医研究所, 动物营养学国家重点实验室, 北京 100193;
    3. 温氏食品集团股份有限公司, 云浮 527400;
    4. 新希望六和股份有限公司, 北京 100102;
    5. 唐人神集团股份有限公司, 株洲 412000;
    6. 青岛蔚蓝生物股份有限公司, 青岛 266000;
    7. 湖南中本智能科技有限公司, 长沙 410013
张晋源(1997-),男,重庆人,硕士研究生,从事饲料养分生物学效价评定的研究。E-mail:1151615822@qq.com

收稿日期: 2020-11-24

  网络出版日期: 2021-06-10

基金资助

国家自然科学基金项目（31501977）；西南民族大学中央高校基本科研业务费专项资金项目（2021PYXM01）；温氏食品集团股份有限公司合作项目（2018-YF-01）；新希望六和股份有限公司合作项目（2020-YF-04）

收起

Consistency and Additivity in Gross Energy of Monogastric Animal Feed Samples between Different Laboratories

  • ZHANG Jinyuan ,
  • WANG Yuming ,
  • DU Zhongyuan ,
  • DONG Ying ,
  • LI Dailin ,
  • WANG Yueli ,
  • HUANG Qinghua ,
  • DU Qingzhi ,
  • HUANG Yanling ,
  • ZHAO Feng
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  • 1. College of Animal and Veterinary Science, Southwest Minzu University, Chengdu 610041, China;
    2. State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China;
    3. Wenshi Food Group Limited Company, Yunfu 527400, China;
    4. New Hope Liuhe Limited Company, Beijing 100102, China;
    5. Tangrenshen Group Limited Company, Zhuzhou 412000, China;
    6. Qingdao Weilan Biology Limited Company, Qingdao 266000, China;
    7. Hunan Zhongben Intelligent Technology Development Limited Company, Changsha 410013, China

Received date: 2020-11-24

  Online published: 2021-06-10

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摘要

本研究旨在比较不同实验室间测定饲料总能（GE）的变异程度及可加性，为饲料有效能值的准确测定提供参考。试验分为2个部分。内容一：考察饲料的上样量对氧弹热量计测定GE值的影响，采用单因素完全随机设计，6个饲料原料分别为玉米、豆粕、小麦麸、大豆油、菜籽粕、棉籽粕，10个饲粮上样量共计5个处理，分别设为0.2、0.4、0.6、0.8和1.0 g，每个处理4个重复。内容二：考察实验室间测定饲粮GE的变异及可加性。采用单因素完全随机设计，共6个实验室，每个实验室对各样品测定4个重复。结果表明：6个饲料原料及10个饲粮GE的测定值均随上样量的增加而呈显著线性和二次曲线增加（P<0.05）。0.2 g上样量的GE测定值均显著低于0.4~1.0 g上样量相应的GE测定值（P<0.05）。在0.6~1.0 g上样量间，样品GE测定值相对差异较小。在6个实验室对比中，96个测定数据中的91个的|Z|<2。h统计量表明，实验室2的测定结果偏低，而实验室5的测定结果偏高。k统计量表明，实验室5和6的测定变异相对较大。总体上，GE测定的重复性变异系数、实验室间变异系数及再现性变异系数不超过0.64%。配对t检验表明，实验室1、3、5和6对10个饲粮GE的计算值与测定值差异不显著。而实验室2和4对10个饲粮GE的计算值与测定值差异显著（P<0.05）。6个实验室内饲粮的GE测定值对计算值的线性回归模型与Y=X重叠。由此表明，氧弹热量计测定GE的上样量应在0.6 g以上。6个实验室间测定饲粮GE值呈现较好的一致性（94.8%的满意度），实验室间的变异系数均较低。4个实验室测定饲粮GE的可加性高，2个实验室的可加性稍低。

关键词： 饲料; 总能; 变异系数; 可加性

本文引用格式

张晋源 , 王钰明 , 杜中原 , 董莹 , 李黛淋 , 王跃丽 , 黄庆华 , 杜青之 , 黄艳玲 , 赵峰 . 不同实验室间测定单胃动物饲料样品总能的一致性及可加性研究[J]. 动物营养学报, 2021 , 33(6) : 3581 -3591 . DOI: 10.3969/j.issn.1006-267x.2021.06.060

Abstract

The objective of this study was to compare the variation and additivity of gross energy (GE) measured in different laboratories, which will provide a reference for accurately determining effective energy value of feed. This experiment consisted of two parts. Part 1 was to investigate the effects of the feed sample volumes on the GE value determined by the oxygen bomb calorimeter. A single factor completely random design for sample volumes at 0.2, 0.4, 0.6, 0.8 and 1.0 g was adopted for each of 6 feed ingredients (corn, soybean meal, wheat bran, soybean oil, rapeseed meal and cottonseed meal) and 10 diets. Each treatment contained 4 replicates. Part 2 was to investigate the variation and additivity of GE in feed determined in different laboratories. A single factor completely random design for 6 laborotaries was adopted for each feed. The GE of each sample was measured for 4 replicates in each laboratory. The results showed that the measured GE of 6 feed ingredients and 10 diets increased linearly and quadraticly with the increment of sample volumes (P<0.05). The measured GE values at sample volume of 0.2 g were lower than the GE values measured at sample volume of 0.4 to 1.0 g (P<0.05). Relatively little difference was observed in GE measured at sample volumes of 0.6 to 1.0 g. In the comparison among 6 laboratories, the absoute Z scores of 91 out of 96 measured GE were less than 2. The h statistics showed that the measured GE in laboratory 2 was lower, whereas the measured GE in laboratory 5 was higher than other laboratories. The k statistics showed that the variation of GE measured in laboratory 5 and 6 was relatively greater. In total, the coefficient of variation for repeatability, inter-laboratory and reproducibility of GE didn't exceed 0.64%. The paired t test showed that no significant differences between the calculated and measured GE of 10 diets were observed in each of laboratory 1, 3, 5 and 6. Whereas, significant differences between the calculated and measured GE of 10 diets were observed in each of laboratory 2 and 4 (P<0.05). The linear regression model of measured on calculated GE in each of 6 laboratories all overlaped with Y=X. This indicates that the loading volumes of sample for GE measurement using the oxygen bomb calorimeter should be more than 0.6 g. The GE value of feed measured in 6 laboratories show good consistency (94.8% satisfaction), and low coefficient of variation is observed in GE measured among laboratories. Higher and high additivity of dietary GE are present in laboratory 4 and 2, respectively.

Key words： feed; gross energy; coefficient of variation; additivity

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