综述 REVIEW

乳酸菌胞外多糖对动物肠道屏障功能的调控作用及机制

  • 王琪 ,
  • 肖融 ,
  • 王敬 ,
  • 齐仁立
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  • 重庆市畜牧科学院, 重庆 402460
王琪(1984-),女,四川自贡人,助理研究员,硕士,主要从事动物肠道微生物调控机制研究。E-mail:wangq0418@126.com

收稿日期: 2020-11-26

  网络出版日期: 2021-07-06

基金资助

重庆市自然科学基金项目(cstc2018jcyjAX0323);重庆市财政专项资金项目(19506)

Regulation and Mechanism of Lactic Acid Bacteria Exopolysaccharide on Intestinal Barrier Function of Animals

  • WANG Qi ,
  • XIAO Rong ,
  • WANG Jing ,
  • QI Renli
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  • Chongqing Academy of Animal Sciences, Chongqing 402460, China

Received date: 2020-11-26

  Online published: 2021-07-06

Supported by

 

摘要

肠道屏障是阻止肠道病原体入侵的第1道屏障,包括机械屏障、化学屏障、微生物屏障以及免疫屏障。胞外多糖是乳酸菌在其生长代谢过程中,分泌到细胞壁外及周围生长介质中的一类生物大分子物质。研究证实,乳酸菌胞外多糖可增强动物肠道屏障功能,但通过何种具体机制发挥调节作用尚不清楚。本文概述了乳酸菌胞外多糖对动物肠道屏障功能的调控作用及相关机制,为其在动物生产中的应用提供参考。

本文引用格式

王琪 , 肖融 , 王敬 , 齐仁立 . 乳酸菌胞外多糖对动物肠道屏障功能的调控作用及机制[J]. 动物营养学报, 2021 , 33(7) : 3657 -3664 . DOI: 10.3969/j.issn.1006-267x.2021.07.007

Abstract

The intestinal barrier is the first barrier to prevent the invasion of pathogens, mainly including the intestinal mechanical barrier, chemical barrier, microbial barrier and immune barrier. Exopolysaccharides are a class of biological macromolecules which secreted by lactic acid bacteria to the outside of cell wall and growth medium during its growth and metabolism process. Lactic acid bacteria exopolysaccharides have been confirmed that it can enhance intestinal barrier function, but its regulatory mechanism is still unclear. This paper summarizes the effects of lactic acid bacteria exopolysaccharides on intestinal barrier function and its molecular mechanisms, and aims to provide information for its application in animal production.

参考文献

[1] HIIPPALA K,JOUHTEN H,RONKAINEN A,et al.The potential of gut commensals in reinforcing intestinal barrier function and alleviating inflammation[J].Nutrients,2018,10(8):988.
[2] VAUGHAN E E,DE VRIES M C,ZOETENDAL E G,et al.The intestinal LABs[M]//SIEZEN R J,KOK J,ABEE T.Lactic acid bacteria:genetics,metabolism and applications.Dordrecht:Springer,2002:341-352.
[3] WANG M J,WU H Q,LU L H,et al.Lactobacillus reuteri promotes intestinal development and regulates mucosal immune function in newborn piglets[J].Frontiers in Veterinary Science,2020,7:42.
[4] XIE J H,YU Q,NIE S P,et al.Effects of Lactobacillus plantarum NCU116 on intestine mucosal immunity in immunosuppressed mice[J].Journal of Agricultural and Food Chemistry,2015,63(51):10914-10920.  
[5] POLAK-BERECKA M,WAŚKO A,SKRZYPEK H,et al.Production of exopolysaccharides by a probiotic strain of Lactobacillus rhamnosus:biosynthesis and purification methods[J].Acta Alimentaria,2013,42(2):220-228.  
[6] VINDEROLA G,PERDIGÓN G,DUARTE J,et al.Effects of the oral administration of the exopolysaccharide produced by Lactobacillus kefiranofaciens on the gut mucosal immunity[J].Cytokine,2006,36(5/6):254-260.
[7] PATTEN D A,LEIVERS S,CHADHA M J,et al.The structure and immunomodulatory activity on intestinal epithelial cells of the EPSs isolated from Lactobacillus helveticus sp. Rosyjski and Lactobacillus acidophilus sp. 5e2[J].Carbohydrate Research,2014,384:119-127.
[8] 张晓黎,吴兴壮,张华.乳酸菌功能作用及其在生产中的应用[J].农业工程技术,2015,14(3):36-39. ZHANG X L,WU X Z,ZHANG H.The function of lactic acid bacteria and its application in production[J].Agricultural Engineering Technology,2015,14(3):36-39.(in Chinese)
[9] SALAZAR N,GUEIMONDE M,DE LOS REYES-GAVILÁN C G,et al.Exopolysaccharides produced by lactic acid bacteria and bifidobacteria as fermentable substrates by the intestinal microbiota[J].Critical Reviews in Food Science and Nutrition,2016,56(9):1440-1453.  
[10] OLEKSY M,KLEWICKA E.Exopolysaccharides produced by Lactobacillus sp.:biosynthesis and applications[J].Critical Reviews in Food Science and Nutrition,2018,58(3):450-462.
[11] GERWIG G J.Structural analysis of exopolysaccharides from lactic acid bacteria[M]//KANAUCHI M.Lactic acid bacteria.New York,NY:Humana Press,2019,1887:67-84.
[12] WANG K,NIU M M,SONG D W,et al.Preparation,partial characterization and biological activity of exopolysaccharides produced from Lactobacillus fermentum S1[J].Journal of Bioscience and Bioengineering,2020,129(2):206-214.  
[13] XU Y M,CUI Y L,WANG X,et al.Purification,characterization and bioactivity of exopolysaccharides produced by Lactobacillus plantarum KX041[J].International Journal of Biological Macromolecules,2019,128:480-492.
[14] CHEN H X,ZHANG M,QU Z S,et al.Antioxidant activities of different fractions of polysaccharide conjugates from green tea (Camellia sinensis)[J].Food Chemistry,2008,106(2):559-563.  
[15] RAJOKA M S R,MEHWISH H M,HAYAT H F,et al.Characterization,the antioxidant and antimicrobial activity of exopolysaccharide isolated from poultry origin Lactobacilli[J].Probiotics and Antimicrobial Proteins,2019,11(4):1132-1142.  
[16] SHIOMI M,SASAKI K,MUROFUSHI M,et al.Antitumor activity in mice of orally administered polysaccharide from Kefir grain[J].Japanese Journal of Medical Science and Biology,1982,35(2):75-80.  
[17] LIU C T,CHU F J,CHOU C C,et al.Antiproliferative and anticytotoxic effects of cell fractions and exopolysaccharides from Lactobacillus casei 1[J]Mutation Research/Genetic Toxicology and Environmental Mutagenesis,2011,721(2):157-162.
[18] DI W,ZHANG L W,YI H X,et al.Exopolysaccharides produced by Lactobacillus strains suppress HT-29 cell growth via induction of G0/G1 cell cycle arrest and apoptosis[J].Oncology Letters,2018,16(3):3577-3586.
[19] EL-DEEB N M,YASSIN A M,AL-MADBOLY L A,et al.A novel purified Lactobacillus acidophilus 20079 exopolysaccharide,LA-EPS-20079,molecularly regulates both apoptotic and NF-κB inflammatory pathways in human colon cancer[J].Microbial Cell Factories,2018,17:29.
[20] 周兴涛.植物乳杆菌NCU116胞外多糖抗肿瘤及调节肠道屏障功能机制的初探[D].博士学位论文.南昌:南昌大学,2019:39-55. ZHOU X T.Investigation on the mechanism of anti-tumor and intestinal barrier function regulation by exopolysaccharides from Lactobacillus plantarum NCU116[D].Ph.D.Thesis.Nanchang:Nanchang University,2018:39-55.(in Chinese)
[21] PARISA A,ROYA G,MAHDI R,et al.Anti-cancer effects of Bifidobacterium species in colon cancer cells and a mouse model of carcinogenesis[J].PLoS One,2020,15(5):e0232930.
[22] SAADAT Y R,KHOSROUSHAHI A Y,GARGARI B P.A comprehensive review of anticancer,immunomodulatory and health beneficial effects of the lactic acid bacteria exopolysaccharides[J].Carbohydrate Polymers,2019,217:79-89.
[23] TANG Y J,DONG W,WAN K Y,et al.Exopolysaccharide produced by Lactobacillus plantarum induces maturation of dendritic cells in BALB/c mice[J].PLoS One,2015,10(11):e0143743.
[24] WANG J,WU T,FANG X B,et al.Characterization and immunomodulatory activity of an exopolysaccharide produced by Lactobacillus plantarum JLK0142 isolated from fermented dairy tofu[J].International Journal of Biological Macromolecules,2018,115:985-993.
[25] KITAZAWA H,HARATA T,UEMURA J,et al.Phosphate group requirement for mitogenic activation of lymphocytes by an extracellular phosphopolysaccharide from Lactobacillus delbrueckii ssp.bulgaricus[J].International Journal of Food Microbiology,1998,40(3):169-175.  
[26] KAWASHIMA T,MURAKAMI K,NISHIMURA I,et al.A sulfated polysaccharide,fucoidan,enhances the immunomodulatory effects of lactic acid bacteria[J].International Journal of Molecular Medicine,2011,29(3):447-453.
[27] BLEAU C,MONGES A,RASHIDAN K,et al.Intermediate chains of exopolysaccharides from Lactobacillus rhamnosus RW-9595M increase IL-10 production by macrophages[J].Journal of Applied Microbiology,2010,108(2):666-675.  
[28] ANDERSON R C,DALZIEL J E,GOPAL P K,et al.The role of intestinal barrier function in early life in the development of colitis[M]//FUKATA.Colitis.London:InTechopen,2012:3-6.
[29] RAMANAN D,CADWELL K.Intrinsic defense mechanisms of the intestinal epithelium[J].Cell Host & Microbe,2016,19(4):434-441.  
[30] ZHOU X T,QI W C,HONG T,et al.Exopolysaccharides from Lactobacillus plantarum NCU116 regulate intestinal barrier function via STAT3 signaling pathway[J].Journal of Agricultural and Food Chemistry,2018,66(37):9719-9727.  
[31] DEL PIANO M,BALZARINI M,CARMAGNOLA S,et al.Assessment of the capability of a gelling complex made of tara gum and the exopolysaccharides produced by the microorganism Streptococcus thermophilus ST10 to prospectively restore the gut physiological barrier:a pilot study[J].Journal of Clinical Gastroenterology,2014,48(Suppl.1):S56-S61.
[32] CHAMBERS E S,MORRISON D J,FROST G.Control of appetite and energy intake by SCFA:what are the potential underlying mechanisms?[J].Proceedings of the Nutrition Society,2014,74(3):328-336.
[33] XU R H,ARUHAN,XIU L,et al.Exopolysaccharides from Lactobacillus buchneri TCP016 attenuate LPS- and d-GalN-induced liver injury by modulating the gut microbiota[J].Journal of Agricultural and Food Chemistry,2019,67(42):11627-11637.  
[34] ZHANG J,ZHAO X,JIANG Y Y,et al.Antioxidant status and gut microbiota change in an aging mouse model as influenced by exopolysaccharide produced by Lactobacillus plantarum YW11 isolated from Tibetan kefir[J].Journal of Dairy Science,2017,100(8):6025-6041.  
[35] JEONG D,KIM D H,KANG I B,et al.Characterization and antibacterial activity of a novel exopolysaccharide produced by Lactobacillus kefiranofaciens DN1 isolated from kefir[J].Food Control,2017,78:436-442.
[36] KONIECZNA C,SŁODZIŃSKI M,SCHMIDT M T.Exopolysaccharides produced by Lactobacillus rhamnosus KL 53A and Lactobacillus casei Fyos affect their adhesion to enterocytes[J].Polish Journal of Microbiology,2018,67(3):273-281.  
[37] SANDERS M E.Impact of probiotics on colonizing microbiota of the gut[J].Journal of Clinical Gastroenterology,2011,45(Suppl.):S115-S119.
[38] RUAS-MADIEDO P,GUEIMONDE M,MARGOLLES A,et al.Exopolysaccharides produced by probiotic strains modify the adhesion of probiotics and enteropathogens to human intestinal mucus[J].Journal of Food Protection,2006,69(8):2011-2015.  
[39] FANNING S,HALL L J,CRONIN M,et al.Bifidobacterial surface-exopolysaccharide facilitates commensal-host interaction through immune modulation and pathogen protection[J].Proceedings of the National Academy of Sciences of the United States of America,2012,109(6):2108-2113.  
[40] CHEN X Y,WOODWARD A,ZIJLSTRA R T,et al.Exopolysaccharides synthesized by Lactobacillus reuteri protect against enterotoxigenic Escherichia coli in piglets[J].Applied and Environmental Microbiology,2014,80(18):5752-5760.  
[41] KŠONŽEKOVÁ P,BYSTRICKÝ P,VLČKOVÁ S,et al.Exopolysaccharides of Lactobacillus reuteri:their influence on adherence of E.coli to epithelial cells and inflammatory response[J].Carbohydrate Polymers,2016,141:10-19.
[42] SHAO L,WU Z J,ZHANG H,et al.Partial characterization and immunostimulatory activity of exopolysaccharides from Lactobacillus rhamnosus KF5[J].Carbohydrate Polymers,2014,107:51-56.
[43] LIU C F,TSENG K C,CHIANG S S,et al.Immunomodulatory and antioxidant potential of Lactobacillus exopolysaccharides[J].Journal of the Science of Food and Agriculture,2011,91(12):2284-2291.
[44] MATSUZAKI C,KAMISHIMA K,MATSUMOTO K,et al.Immunomodulating activity of exopolysaccharide-producing Leuconostoc mesenteroides strain NTM048 from green peas[J].Journal of Applied Microbiology,2014,116(4):980-989.  
[45] MATSUZAKI C,HAYAKAWA A,MATSUMOTO K,et al.Exopolysaccharides produced by Leuconostoc mesenteroides strain NTM048 as an immunostimulant to enhance the mucosal barrier and influence the systemic immune response[J].Journal of Agricultural and Food Chemistry,2015,63(31):7009-7015.  
[46] REN Q Q,TANG Y J,ZHANG L L,et al.Exopolysaccharide produced by Lactobacillus casei promotes the differentiation of CD4+ T Cells into Th17 Cells in BALB/c mouse peyer's patches in vivo and in vitro[J].Journal of Agricultural and Food Chemistry,2020,68(9):2664-2672.  
[47] LAIÑO J,VILLENA J,KANMANI P,et al.Immunoregulatory effects triggered by lactic acid bacteria exopolysaccharides:new insights into molecular interactions with host cells[J].Microorganisms,2016,4(3):27.
[48] MUROFUSHI Y,VILLENA J,MORIE K,et al.The Toll-like receptor family protein RP105/MD1 complex is involved in the immunoregulatory effect of exopolysaccharides from Lactobacillus plantarum N14[J].Molecular Immunology,2015,64(1):63-75.  
[49] GAO K,WANG C,LIU L,et al.Immunomodulation and signaling mechanism of Lactobacillus rhamnosus GG and its components on porcine intestinal epithelial cells stimulated by lipopolysaccharide[J].Journal of Microbiology,Immunology and Infection,2017,50(5):700-713.  
[50] TKA ČC ˇ IKOVA L,MOCHNA C ˇ OVÁ E,TYAGI P,et al.Comprehensive mapping of the cell response to E.coli infection in porcine intestinal epithelial cells pretreated with exopolysaccharide derived from Lactobacillus reuteri[J].Veterinary Research,2020,51:49.
[51] KANMANI P,ALBARRACIN L,KOBAYASHI H,et al.Exopolysaccharides from Lactobacillus delbrueckii OLL1073R-1 modulate innate antiviral immune response in porcine intestinal epithelial cells[J].Molecular Immunology,2018,93:253-265.
[52] KANMANI P,ALBARRACIN L,KOBAYASHI H,et al.Genomic characterization of Lactobacillus delbrueckii TUA4408L and evaluation of the antiviral activities of its extracellular polysaccharides in porcine intestinal epithelial cells[J].Frontiers in Immunology,2018,9:2178.
[53] 包鹏,唐彦君,刘宁.Lactobacillus casei胞外多糖对BALB/c小鼠肠相关淋巴细胞调控的初步研究[J].中国免疫学杂志,2013,29(2):182-186. BAO P,TANG Y J,LIU N,et al.Effect of exopolysaccharide from Lactobacillus casei on murine gut-associated lymphocytes[J].Chinese Journal of Immunology,2013,29(2):182-186.(in Chinese)
[54] LEBEER S,CLAES I J J,VERHOEVEN T L A,et al.Exopolysaccharides of Lactobacillus rhamnosus GG form a protective shield against innate immune factors in the intestine[J].Microbial Biotechnology,2011,4(3):368-374.  
[55] KIM K,LEE G,THANH H D,et al.Exopolysaccharide from Lactobacillus plantarum LRCC5310 offers protection against rotavirus-induced diarrhea and regulates inflammatory response[J].Journal of Dairy Science,2018,101(7):5702-5712.  
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