动物营养学报 >

2021 , Vol. 33 >Issue 7: 3759 - 3767

DOI: https://doi.org/10.3969/j.issn.1006-267x.2021.07.018

猪营养与饲料 SWINE NUTRITION AND FEED

鸟氨酸-α-酮戊二酸对由D-半乳糖导致的慢性氧化应激仔猪生长性能、脏器指数、肠道形态和血清生化指标的影响

  • 王鹏 ,
  • 黎育颖 ,
  • 田军权 ,
  • 苏文璇 ,
  • 尹杰 ,
  • 印遇龙 ,
  • 姚康
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  • 1. 中国科学院亚热带农业生态研究所, 亚热带农业生态过程重点实验室, 湖南省畜禽健康养殖工程技术中心, 长沙 410025;
    2. 中国科学院大学, 北京 100049;
    3. 湖南农业大学动物科学技术学院, 长沙 410128
王鹏(1995-),男,四川南充人,硕士研究生,动物营养与饲料科学专业。E-mail:1029224986@qq.com

收稿日期: 2020-12-29

  网络出版日期: 2021-07-06

基金资助

中国科学院百人计划；中国科学院亚热带农业生态研究所农业生态过程重点实验室基金（ISA2016101）

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Effects of Ornithine-α-Ketoglutarate on Growth Performance, Organ Indexes, Intestinal Morphology and Serum Biochemical Indexes of Piglets with Chronic Oxidative Stress Caused by D-Galactose

  • WANG Peng ,
  • LI Yuying ,
  • TIAN Junquan ,
  • SU Wenxuan ,
  • YIN Jie ,
  • YIN Yulong ,
  • YAO Kang
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  • 1. Hunan Provincial Engineering Research Center for Healthy Breeding of Livestock and Poultry, Key Laboratory of Agroecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410025, China;
    2. University of Chinese Academy of Sciences, Beijing 100049, China;
    3. College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China

Received date: 2020-12-29

  Online published: 2021-07-06

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摘要

本试验旨在研究鸟氨酸-α-酮戊二酸（OKG）对由D-半乳糖导致的慢性氧化应激仔猪生长性能、脏器指数、肠道形态和血清生化指标的影响。试验选取40头健康的"长×大"二元杂交断奶阉公猪，平均体重为（7.68±0.56） kg，按体重相近原则随机分为5组，分别为对照组（基础饲粮）、模型组（基础饲粮+5 g/kg BW D-半乳糖）、SOKG组（基础饲粮+5 g/kg BW D-半乳糖+0.5%OKG）、MOKG组（基础饲粮+5 g/kg BWD-半乳糖+1.0%OKG）和LOKG组（基础饲粮+5 g/kg BW D-半乳糖+2.0%OKG），每组8个重复，每个重复1头猪。试验期为28 d，试验结束后测定仔猪生长性能、脏器指数、肠道形态和血清生化指标。结果表明：1）模型组末重、平均日增重（ADG）和平均日采食量（ADFI）相较于对照组显著降低（P<0.05），料重比（F/G）则显著增加（P<0.05）；SOKG组末重、ADG相较于模型组显著增加（P<0.05），F/G则显著降低（P<0.05）；MOKG组F/G相较于模型组显著降低（P<0.05）。2）MOKG组脾脏指数以及肾脏指数相较于对照组和模型组显著升高（P<0.05）。3）SOKG组空肠绒毛高度显著高于模型组（P<0.05）；MOKG组和LOKG组回肠隐窝深度较模型组有所降低（P>0.05）。4）MOKG组血清总蛋白（TP）含量相较于对照组显著增加（P<0.05）；模型组血清碱性磷酸酶（ALP）活性相较于对照组显著降低（P<0.05），而SOKG组血清ALP活性相较于模型组显著增加（P<0.05）；MOKG组血清免疫球蛋白G（IgG）含量相较于对照组显著增加（P<0.05）；MOKG组和LOKG组血清免疫球蛋白M（IgM）含量相较于对照组和模型组显著增加（P<0.05）。由此可见，OKG可以减轻生长抑制，通过促进脾脏和肾脏生长，改善小肠黏膜形态结构，提高血清TP、IgG和IgM含量与ALP活性，从而缓解由D-半乳糖造成的仔猪慢性氧化应激。

关键词： 鸟氨酸-α-酮戊二酸; D-半乳糖; 慢性氧化应激; 仔猪; 生长性能; 脏器指数; 肠道形态学; 血清生化指标

本文引用格式

王鹏 , 黎育颖 , 田军权 , 苏文璇 , 尹杰 , 印遇龙 , 姚康 . 鸟氨酸-α-酮戊二酸对由D-半乳糖导致的慢性氧化应激仔猪生长性能、脏器指数、肠道形态和血清生化指标的影响[J]. 动物营养学报, 2021 , 33(7) : 3759 -3767 . DOI: 10.3969/j.issn.1006-267x.2021.07.018

Abstract

The aim of this study was to investigate the effects of ornithine-α-ketoglutarate (OKG) on growth performance, organ indexes, intestinal morphology and serum biochemical indexes of piglets with chronic oxidative stress caused by D-galactose. A total of 40 castrated and healthy weaned piglets (Landrace×Yorkshire) with the average body weight of (7.68±0.56) kg were randomly assigned to five groups with 8 repetition per group and 1 piglet per repetition:control group (basal diet), model group (basal diet+5 g/kg BW D-galactose), SOKG group (basal diet+5 g/kg BW D-galactose+0.5% OKG), MOKG group (basal diet+5 g/kg BW D-galactose+1.0% OKG) and LOKG group (basal diet+5 g/kg BW D-galactose+2.0% OKG). The experimental lasted for 28 days. At the end of the experiment, the piglet's growth performance, organ indexes, intestinal morphology and serum biochemical indexes were determined. The results showed as follows:1) the final body weight, average daily gain (ADG) and average daily feed intake (ADFI) of the model group were significantly lower, and the feed/gain (F/G) was significantly higher than those of the control group (P<0.05); the final body weight and ADG of the SOKG group were significantly higher, and the F/G was significantly lower than that of the model group (P<0.05); the F/G of the MOKG group was significantly lower than that of the model group (P<0.05). 2) The spleen index and kidney index of the MOKG group were significantly higher than those of the control group and the model group (P<0.05). 3) The jejunal villus height of the SOKG group was significantly higher than that of the model group (P<0.05); the ileal crypt depth in the MOKG group and LOKG group was lower than that of the model group (P>0.05). 4) Serum total protein (TP) content of the MOKG group was significantly increased compared with the control group (P<0.05); serum alkaline phosphatase (ALP) activity of the model group was significantly decreased compared with the control group (P<0.05), while serum ALP activity of the SOKG group was significantly increased compared with the model group (P<0.05); serum immunoglobulin G (IgG) content of the MOKG group was significantly increased compared with the control group (P<0.05); serum immunoglobulin M (IgM) content of the MOKG group and LOKG group was significantly increased compared with the control group and model group (P<0.05). Collectively, these results indicate that OKG can alleviate the growth-suppression, promote the spleen and kidney growth, improve the morphological structure of intestinal mucosa, increase the serum TP, IgG, IgM contents and ALP activity, and alleviate the chronic oxidative stress of piglets caused by D-galactose.

Key words： ornithine-α-ketoglutarate; D-galactose; chronic oxidative stress; piglets; growth performance; organ indexes; intestinal morphology; serum biochemical indexes

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