禽营养与饲料 POULTRY NUTRITION AND FEED

饲粮添加一水肌酸对宰前运输应激黔东南小香鸡血液指标、肉品质及肌肉糖酵解的影响

  • 张柏林 ,
  • 刘宁 ,
  • 李家惠 ,
  • 王双双 ,
  • 郝美林 ,
  • 王庆容 ,
  • 练凤仙 ,
  • 胡建琴
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  • 遵义师范学院生物与农业科技学院, 赤水河流域特色动物资源保护与利用重点实验室, 遵义 523006
张柏林(1981-),男,山东烟台人,副教授,博士,研究方向为动物营养与生理调控。E-mail:bolin-zhang@163.com

收稿日期: 2021-01-07

  网络出版日期: 2021-07-06

基金资助

赤水河流域资源保护与开发研究院开放基金项目(遵师CSHKJ[2019]-01号);国家自然科学基金项目(31760674);遵义市"15851"人才项目(2050020213#);遵义市校联合基金(遵市科合计[2018]08号);贵州省服务农村产业革命项目(遵师合农村产业字201905)

Effects of Dietary Creatine Monohydrate on Blood Indexes, Meat Quality and Muscle Glycolysis of Qiandongnan Xiaoxiang Chickens Subjected to Pre-Slaughter Transportation Stress

  • ZHANG Bolin ,
  • LIU Ning ,
  • LI Jiahui ,
  • WANG Shuangshuang ,
  • HAO Meilin ,
  • WANG Qingrong ,
  • LIAN Fengxian ,
  • HU Jianqin
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  • Key Laboratory of Conservation and Utilization of Characteristic Animal Resources in Chishui River Basin, Department of Biology and Agriculture, Zunyi Normal university, Zunyi 523006, China

Received date: 2021-01-07

  Online published: 2021-07-06

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摘要

本试验旨在研究饲粮添加一水肌酸(CMH)对宰前运输应激黔东南小香鸡血液指标、肉品质及肌肉糖酵解的影响。试验选择150日龄、体重相近健康的黔东南小香鸡160羽,随机分成4个组,每组20个重复,每个重复2只。A组和B组饲喂基础饲粮,C组和D组分别在基础饲粮中添加0.06%和0.12%的CMH。试验期为14 d。试验结束当天,A组不做运输应激处理,B组、C组和D组进行3 h宰前运输应激处理。结果表明:1)饲粮添加CMH对黔东南小香鸡平均日增重、平均日采食量及料重比均无显著影响(P>0.05)。2)与A组相比,B组的血清皮质酮含量显著增加(P<0.05),血清葡萄糖含量显著降低(P<0.05),胸肌pH24 h、糖原含量显著降低(P<0.05),胸肌亮度(L*)值、滴水损失、蒸煮损失、乳酸含量及糖酵解潜力显著增加(P<0.05),胸肌丙酮酸激酶、己糖激酶和果糖-2,6-二磷酸酶活性显著增加(P<0.05)。3)与B组相比,D组的血清葡萄糖含量显著增加(P<0.05),血清皮质酮含量显著降低(P<0.05),胸肌pH24 h和糖原含量显著增加(P<0.05),胸肌丙酮酸激酶、己糖激酶和果糖-2,6-二磷酸酶活性显著降低(P<0.05),胸肌L*值、滴水损失、蒸煮损失、乳酸含量及糖酵解潜力显著降低(P<0.05)。综上所述,饲粮添加0.12%的CMH缓解了黔东南小香鸡运输应激,在一定程度上有效抑制了运输应激引起的肌肉糖酵解,对宰前运输应激黔东南小香鸡肌肉品质有一定的改善作用。

本文引用格式

张柏林 , 刘宁 , 李家惠 , 王双双 , 郝美林 , 王庆容 , 练凤仙 , 胡建琴 . 饲粮添加一水肌酸对宰前运输应激黔东南小香鸡血液指标、肉品质及肌肉糖酵解的影响[J]. 动物营养学报, 2021 , 33(7) : 3768 -3777 . DOI: 10.3969/j.issn.1006-267x.2021.07.019

Abstract

The purpose of this experiment was to study the effects of dietary creatine monohydrate (CMH) on blood indexes, meat quality and muscle glycolysis of Qiandongnan Xiaoxiang chickens subjected to pre-slaughter transportation stress. A total of 160 healthy 150-day-old Qiandongnan Xiaoxiang chickens with similar body weight were randomly divided into 4 groups with 20 replicates per group and 2 chickens per replicate. Chickens in group A and group B were fed basal diets, and broilers in group C and group D were fed basal diets supplemented with 0.06% and 0.12% CMH, respectively. The experiment lasted for 14 days. At the end of the experiment, broilers in group A were treated with no transportation stress, and broilers in group B, group C and group D were treated with 3 h pre-slaughter transportation stress. The results showed as follows:1) dietary CMH had no significant effects on the average daily gain, average daily feed intake and the ratio of feed to gain of Qiandongnan Xiaoxiang chickens (P>0.05). 2) Compared with group A, the serum corticosterone content of group B was significantly increased (P<0.05), the serum glucose content was significantly decreased (P<0.05), the breast muscle pH24 h and the glycogen content were significantly decreased (P<0.05), the lightness (L*) value, drip loss, cooking loss, lactic acid content and glycolysis potential in breast muscle were significantly increased (P<0.05), and the activities of pyruvate kinase, hexokinase and fructose-2,6-diphosphate in breast muscle were significantly increased (P<0.05). 3) Compared with group B, the serum glucose content of group D was significantly increased (P<0.05), the serum corticosterone content was significantly decreased (P<0.05), the breast muscle pH24 h and the glycogen content were significantly increased (P<0.05), the activities of pyruvate kinase, hexokinase and fructose-2,6-diphosphate in breast muscle were significantly decreased (P<0.05), and the L* value, drip loss, cooking loss, lactic acid content and glycolysis potential in breast muscle were significantly decreased (P<0.05). In conclusion, dietary supplemented with 0.12% CMH can alleviate the transportation stress of Qiandongnan Xiaoxiang chickens, effectively inhibit the muscle glycolysis induced by transportation stress, and improve the meat quality of Qiandongnan Xiaoxiang chickens subjected to pre-slaughter transportation stress.

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