禽营养与饲料 POULTRY NUTRITION AND FEED

大蒜粉对肉鸡生长性能、抗氧化能力、免疫力和肠道形态的影响

  • 余洋 ,
  • 沈媛媛 ,
  • 杨彩梅 ,
  • 刘金松 ,
  • 曾新福 ,
  • 冯杰
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  • 1. 浙江农林大学动物科技学院·动物医学院, 浙江省畜禽绿色生态健康养殖应用技术研究重点实验室, 动物健康互联网检测技术浙江省工程实验室, 临安 311300;
    2. 浙江万方生物科技有限公司, 安吉 313300;
    3. 浙江大学动物科学学院, 杭州 310058
余洋(1996-),女,重庆人,硕士研究生,研究方向为动物营养与饲料科学。E-mail:2235356131@qq.com

收稿日期: 2020-12-07

  网络出版日期: 2021-07-06

基金资助

浙江省重点研发项目(2019C02051);国家重点研发计划政府间国际创新合作项目(2018YFE0112700)

Effects of Garlic Powder on Growth Performance, Antioxidant Ability, Immunity and Intestinal Morphology of Broilers

  • YU Yang ,
  • SHEN Yuanyuan ,
  • YANG Caimei ,
  • LIU Jinsong ,
  • ZENG Xinfu ,
  • FENG Jie
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  • 1. Key Laboratory of Applied Technology on Green-Eco Healthy Animal Husbandry of Zhejiang Province, Zhejiang Provincial Engineering Laboratory for Animal Health and Internet Technology, College of Animal Sci-Technology and Animal Medicine, Zhejiang Agriculture and Forestry University, Lin'an 311300, China;
    2. Zhejiang Wanfang Biotechnology Co., Ltd., Anji 313300, China;
    3. College of Animal Science and Technology, Zhejiang University, Hangzhou 310058, China

Received date: 2020-12-07

  Online published: 2021-07-06

Supported by

 

摘要

本试验旨在研究大蒜粉对肉鸡生长性能、抗氧化能力、免疫力和肠道形态的影响。选取480羽体重相近、健康状况良好的1日龄白羽肉鸡,随机分成4组,每组8个重复,每个重复15羽,进行为期42 d的饲养试验。对照组饲喂基础饲粮,抗生素组饲喂基础饲粮+75 mg/kg金霉素,2个大蒜粉组分别饲喂基础饲粮+1 000 mg/kg大蒜粉(1 000 mg/kg大蒜粉组)、基础饲粮+2 000 mg/kg大蒜粉(2 000 mg/kg大蒜粉组)。结果表明:1)与对照组相比,饲粮中添加1 000 mg/kg大蒜粉可显著提高1~42日龄肉鸡的平均日增重(P<0.05),添加1 000和2 000 mg/kg大蒜粉可显著降低1~21日龄肉鸡的料重比(P<0.05);2)与对照组和抗生素组比,饲粮中添加1 000和2 000 mg/kg大蒜粉均可显著提高肉鸡血清免疫球蛋白A(IgA)、免疫球蛋白Y(IgY)、免疫球蛋白M(IgM)含量(P<0.05),显著降低血清肿瘤坏死因子-α(TNF-α)含量(P<0.05),且大蒜粉组血清白细胞介素-1β(IL-1β)含量显著低于抗生素组(P<0.05);3)2 000 mg/kg大蒜粉组血清谷胱甘肽过氧化物酶(GSH-Px)活性显著高于对照组(P<0.05);4)与对照组相比,饲粮中添加1 000 mg/kg大蒜粉可显著提高肉鸡21日龄绒毛高度,添加1 000和2 000 mg/kg大蒜粉均可显著降低42日龄肉鸡隐窝深度(P<0.05),并可显著提高绒毛高度/隐窝深度(V/C)(P<0.05);5)与对照组相比,饲粮中添加1 000和2 000 mg/kg大蒜粉均可显著降低回肠黏膜Toll样受体2(TLR2)的相对表达量(P<0.05)。综上可知,饲粮中添加大蒜粉能促进肉鸡生长,调节机体免疫力,促进肠道发育,减少炎症反应。本试验中,肉鸡饲粮中大蒜粉的最佳添加量为1 000 mg/kg。

本文引用格式

余洋 , 沈媛媛 , 杨彩梅 , 刘金松 , 曾新福 , 冯杰 . 大蒜粉对肉鸡生长性能、抗氧化能力、免疫力和肠道形态的影响[J]. 动物营养学报, 2021 , 33(7) : 3790 -3798 . DOI: 10.3969/j.issn.1006-267x.2021.07.021

Abstract

This experiment was conducted to study the effects of garlic powder on the growth performance, antioxidant ability, immunity and intestinal morphology of broilers. A total of 480 healthy 1-day-old white-feathered broilers with the similar body weight were randomly divided into 4 groups with 8 replicates per group and 15 birds per replicate, and a 42-day feeding trial was conducted. Broilers in control group were fed a basal diet, those in antibiotic group were fed the basal diet+75 mg/kg chlortetracycline, those in 1 000 mg/kg garlic powder group were fed the basal diet+1 000 mg/kg garlic powder, and those in 2 000 mg/kg garlic powder group were fed the basal diet+2 000 mg/kg garlic powder. The results showed as follows:1) compared with the control group, the average daily gain of broilers at 1 to 42 days of age was significantly increased by adding 1 000 mg/kg garlic powder (P<0.05), and the feed to gain ratio of broilers at 1 to 21 days of age was significantly decreased by adding 1 000 and 2 000 mg/kg garlic powder (P<0.05). 2) Compared with the control group and the antibiotic group, adding 1 000 and 2 000 mg/kg garlic powder could significantly increase the contents of immunoglobulin A (IgA), immunoglobulin Y (IgY) and immunoglobulin M (IgM) in serum (P<0.05), and significantly decrease serum tumor necrosis factor-α (TNF-α) content (P<0.05). The serum interleukin-1β (IL-1β) content in the two garlic powder groups was significantly decreased compared with the antibiotic group (P<0.05). 3) The serum glutathione peroxidase (GSH-Px) activity in the 2 000 mg/kg garlic powder group was significantly higher than that in the control group (P<0.05). 4) Compared with the control group, adding 1 000 mg/kg garlic powder could significantly increase the villus height of 21-day-old broilers, and adding 1 000 and 2 000 mg/kg garlic powder could significantly decrease the crypt depth and significantly increase the ratio of villus height to crypt depth (V/C) of 42-day-old broilers (P<0.05). 5) Compared with the control group, adding 1 000 and 2 000 mg/kg garlic powder could significantly decrease the relative expression level of Toll-like receptor 2 (TLR2) in ileum mucosa (P<0.05). These results indicate that adding garlic powder in the diet can promote the growth, regulate the immunity, promote the intestinal development, and reduce the inflammatory reaction of broilers. The best addition of garlic powder in the broilers' diet is 1 000 mg/kg in this experiment.

参考文献

[1] 贾江滨,刘静,谭亚非.大蒜化学成分研究进展[J].广东药学,1999,9(1):1-5. JIA J B,LIU J,TAN Y F.Research progress on chemical constituents of garlic[J].Guangdong Pharmacy,1999(1):1-5.(in Chinese)
[2] 苏贝婷,苏振丹,张宿荣.大蒜素抗菌作用机制的研究进展[J].中华生物医学工程杂志,2020,26(2):187-192. SU B T,SU Z D,ZHANG S R.Research progress on antibacterial mechanism of allicin[J].Chinese Journal of Biomedical Engineering,2020,26(2):187-192.(in Chinese)
[3] ROUF R,UDDIN S J,SARKER D K,et al.Antiviral potential of garlic (Allium sativum) and its organosulfur compounds:a systematic update of pre-clinical and clinical data[J].Trends in Food Science & Technology,2020,104:219-234.
[4] ELOSTA A,SLEVIN M,RAHMAN K,et al.Aged garlic has more potent antiglycation and antioxidant properties compared to fresh garlic extract in vitro[J].Scientific Reports,2017,7:39613.
[5] NICASTRO H L,ROSS S A,MILNER J A.Garlic and onions:their cancer prevention properties[J].Cancer Prevention Research,2015,8(3):181-189.  
[6] AYRLE H,NATHUES H,BIEBER A,et al.Placebo-controlled study on the effects of oral administration of Allium sativum L in postweaning piglets[J].Veterinary Record,2019,184(10):316.
[7] SHOJAI T M,LANGEROUDI A G,KARIMI V,et al.The effect of Allium sativum (garlic) extract on infectious bronchitis virus in specific pathogen free embryonic egg[J].Avicenna Journal of Phytomedicine,2016,6(4):458-467.
[8] ELMOWALID G A,EL-HAMID M I A,EL-WAHAB A M A,et al.Garlic and ginger extracts modulated broiler chicks innate immune responses and enhanced multidrug resistant Escherichia coli O78 clearance[J].Comparative Immunology,Microbiology and Infectious Diseases,2019,66:101334.
[9] PEINADO M J,RUIZ R,ECHÁVARRI A,et al.Garlic derivative propyl propane thiosulfonate is effective against broiler enteropathogens in vivo[J].Poultry Science,2012,91(9):2148-2157.  
[10] OLOBATOKE R Y,MULUGETA S D.Effect of dietary garlic powder on layer performance,fecal bacterial load,and egg quality[J].Poultry Science,2011,90(3):665-670.  
[11] BAI S P,WU A M,DING X M,et al.Effects of probiotic-supplemented diets on growth performance and intestinal immune characteristics of broiler chickens[J].Poultry Science,2013,92(3):663-670.  
[12] RASHID I.由Toll like receptors介导的布拉氏酵母菌和枯草芽孢秆菌B10对肉鸡肠黏膜免疫功能影响的研究[D].博士学位论文.杭州:浙江大学,2013. RASHID I.Effects of Saccharomyces boulardii and Bacillus subtilis B10 on intestinal immunity of broiler mediated by Toll like receptors signaling pathway[D].Ph.D.Thesis.Hangzhou:Zhejiang University,2013.(in Chinese)
[13] 程洋.感染不同毒力新城疫病毒后鸡免疫器官Toll样受体及炎症相关基因的表达差异[D].硕士学位论文.雅安:四川农业大学,2014. CHEN Y.Different expression of Toll-like receptor genes and inflammatory related genes between lymphoid organs of Newcastle disease virus F48E9 infected and Mukteswar infected chickens[D].Master's Thesis.Ya'an:Sichuan Agricultural University,2014.(in Chinese)
[14] 朱沛霁.枯草芽孢杆菌对雪山鸡生产性能、肠道健康和免疫机能的影响及机制[D].博士学位论文.扬州:扬州大学,2017. ZHU P J.Effects of dietary supplementation of Bacillus subtilis on growth performance,intestinal health and immune functional responses of Xueshan broiler[D].Ph.D.Thesis.Yangzhou:Yangzhou University,2017.(in Chinese)
[15] 刘慧卿,张代.大蒜多糖对断奶仔猪生产性能和免疫功能的影响[J].中兽医学杂志,2020(8):9-11. LIU H Q,ZHANG D.Effects of garlic polisaccharide on growth performance and immune function of weaned piglets[J]].Chinese Journal of Traditional Veterinary Science,2020(8):9-11.(in Chinese)
[16] KARANGIYA V K,SAVSANI H H,PATIL S S,et al.Effect of dietary supplementation of garlic,ginger and their combination on feed intake,growth performance and economics in commercial broilers[J].Veterinary World,2016,9(3):245-250.  
[17] WANG J X,PENG K M.Developmental morphology of the small intestine of African ostrich chicks[J].Poultry Science,2008,87(12):2629-2635.  
[18] CZERNICHOW B,GALLUSER M,CUI S Q,et al.Comparison of enteral or parenteral administration of medium chain triglycerides on intestinal mucosa in adult rats[J].Nutrition Research,1996,16(5):797-804.  
[19] MISHRA B,JHA R.Oxidative stress in the poultry gut:potential challenges and interventions[J].Frontiers in Veterinary Science,2019,6:60.
[20] BOREK C.Antioxidant health effects of aged garlic extract[J].The Journal of Nutrition,2001,131(Suppl.3):1010S-1015S.
[21] IDE N,LAU B H S.Garlic compounds minimize intracellular oxidative stress and inhibit nuclear factor-κB activation[J].The Journal of Nutrition,2001,131(Suppl.3):1020S-1026S.
[22] LEBACQ-VERHEYDEN A M,VAERMAN J P,HEREMANS J F.Quantification and distribution of chicken immunoglobulins IgA,IgM and IgG in serum and secretions[J].Immunology,1974,27(4):683-692.
[23] LIU C T,SU H M,LII C K,et al.Effect of supplementation with garlic oil on activity of Th1 and Th2 lymphocytes from rats[J].Planta Medica,2009,75(3):205-210.  
[24] KEISS H P,DIRSCH V M,HARTUNG T,et al.Garlic (Allium sativum L.) modulates cytokine expression in lipopolysaccharide-activated human blood thereby inhibiting NF-κB activity[J].The Journal of Nutrition,2003,133(7):2171-2175.  
[25] TAKEDA K,AKIRA S.Toll-like receptors in innate immunity[J].International Immunology,2005,17(1):1-14.
[26] SIMON K,DE VRIES REILINGH G,KEMP B,et al.Development of ileal cytokine and immunoglobulin expression levels in response to early feeding in broilers and layers[J].Poultry Science,2014,93(12):3017-3027.  
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