禽营养与饲料 POULTRY NUTRITION AND FEED

酶解青蒿联合地衣芽孢杆菌对肉仔鸡生长性能和盲肠菌群的影响

  • 金瑶瑶 ,
  • 徐彬 ,
  • 王琳燚 ,
  • 孙全友 ,
  • 席燕燕 ,
  • 袁艳枝 ,
  • 王改利 ,
  • 付趁 ,
  • 李绍钰
展开
  • 1. 河南农业大学动物科技学院, 郑州 450002;
    2. 河南省农业科学院畜牧兽医研究所, 郑州 450002
金瑶瑶(1996-),女,河南洛阳人,硕士研究生,研究方向为动物营养与饲料科学。E-mail:1298242440@qq.com

收稿日期: 2020-12-09

  网络出版日期: 2021-07-06

基金资助

国家肉鸡产业技术体系项目(CARS-41-G19)

Effects of Enzymatically Treated Artemisia annua Combined with Bacillus licheniformis on Growth Performance and Cecal Microbiota of Broilers

  • JIN Yaoyao ,
  • XU Bin ,
  • WANG Linyi ,
  • SUN Quanyou ,
  • XI Yanyan ,
  • YUAN Yanzhi ,
  • WANG Gaili ,
  • FU Chen ,
  • LI Shaoyu
Expand
  • 1. Collage of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450002, China;
    2. Institute of Animal Husbandry and Veterinary Science, Henan Academy of Agricultural Science, Zhengzhou 450002, China

Received date: 2020-12-09

  Online published: 2021-07-06

Supported by

 

摘要

本试验旨在研究酶解青蒿联合地衣芽孢杆菌饲喂肉仔鸡对其生长性能和盲肠菌群的影响。选取1日龄科宝(Cobb)肉仔鸡480只,随机分为4组(每组6个重复,每个重复20只):对照组饲喂基础饲粮,抗生素组、试验Ⅰ组和试验Ⅱ组分别在基础饲粮中添加20 mg/kg抗生素(主要成分为维吉尼霉素)、1 g/kg酶解青蒿以及1 g/kg酶解青蒿+200 mg/kg地衣芽孢杆菌。试验期42 d。结果表明:1)与对照组相比,试验Ⅱ组肉仔鸡平均日增重显著提高(P<0.05),抗生素组和试验Ⅰ组平均日增重分别提高5.18%和7.49%(P>0.05);试验Ⅱ组肉仔鸡终末体重显著提高(P<0.05),抗生素组和试验Ⅰ组分别提高6.94%和6.94%(P>0.05)。各组肉仔鸡平均日采食量和料重比差异不显著(P>0.05)。2)对肉仔鸡盲肠菌群进行alpha多样性分析发现,抗生素组和各试验组盲肠菌群多样性均有所提高(P<0.05)。对4组肉仔鸡盲肠菌群组成进行分析显示,在门水平上,优势菌门主要为厚壁菌门(Firmicutes)、拟杆菌门(Bacteroidetes)和软壁菌门(Tenericutes)。与对照组相比,抗生素组和各试验组厚壁菌门相对丰度显著提高(P<0.05),试验Ⅰ组和试验Ⅱ组拟杆菌门相对丰度显著降低(P<0.05)。在属水平上,与对照组相比,抗生素组和各试验组拟杆菌属相对丰度显著降低(P<0.05),试验Ⅰ组和试验Ⅱ组粪杆菌属相对丰度显著提高(P<0.05),试验Ⅱ组瘤胃球菌科UCG-014菌属相对丰度显著提高(P<0.05)。由此得出,酶解青蒿用于肉仔鸡饲粮具有替代抗生素的潜质。饲粮单独添加酶解青蒿具有改善肉仔鸡生长性能的趋势,酶解青蒿和地衣芽孢杆菌联用可通过改善盲肠菌群结构,提高肠道菌群多样性,以及提高厚壁菌门与拟杆菌门相对丰度的比例来提高肉鸡生长性能。

本文引用格式

金瑶瑶 , 徐彬 , 王琳燚 , 孙全友 , 席燕燕 , 袁艳枝 , 王改利 , 付趁 , 李绍钰 . 酶解青蒿联合地衣芽孢杆菌对肉仔鸡生长性能和盲肠菌群的影响[J]. 动物营养学报, 2021 , 33(7) : 3810 -3820 . DOI: 10.3969/j.issn.1006-267x.2021.07.023

Abstract

This experiment was conducted to investigate the effects of dietary enzymatically treated Artemisia annua combined with Bacillus licheniformis on the growth performance and cecal microbiota of broilers. A total of 480 Cobb broilers of one-day-old were randomly divided into 4 groups with 6 replicates per group and 20 broilers per replicate. Broilers in the control group were fed a basal diet, and those in the antibiotic group, trial group Ⅰ and trial group Ⅱ were fed the basal diets supplemented with 20 mg/kg antibiotics (a kind of virginiamycin premix), 1 g/kg enzymatically treated Artemisia annua and 1 g/kg enzymatically treated Artemisia annua+200 mg/kg Bacillus licheniformis, respectively. The experiment lasted for 42 days. The results showed as follows:1) compared with the control group, the average daily weight gain of broilers in the trial group Ⅱ was significantly increased (P<0.05), and which in the antibiotic group and trial group Ⅰ was increased by 5.18% and 7.49% (P>0.05), respectively; the final body weight of broilers in the trial group Ⅱ was significantly increased (P<0.05), and which in the antibiotic group and trial group Ⅰ was increased by 6.94% and 6.94% (P>0.05), respectively. There was no significant difference in average daily feed intake and the ratio of feed to gain among all groups (P>0.05). 2) The alpha diversity analysis in cecal microbiota of broilers found that the cecal microbiota diversity in the antibiotic group and both trial groups were significantly improved (P<0.05). The composition analysis in cecal microbiota of broilers in all 4 groups found that, at the phylum level, the dominant microbial phyla were mainly Firmicutes, Bacteroidetes and Tenericutes. Compared with the control group, the Firmicutes relative abundance in the antibiotic group and both trial groups was significantly increased (P<0.05), and the Bacteroidetes relative abundance in the trial group Ⅰ and trial groupⅡ was significantly decreased (P<0.05). At the genus level, compared with the control group, the Bacteroides relative abundance in the antibiotic group and both trial groups was significantly reduced (P<0.05), the Faecalibacterium relative abundance in the trial group Ⅰ and trial group Ⅱ was significantly increased (P<0.05), and the Ruminococcaceae_UCG-014 relative abundance in the trial group Ⅱ was significantly increased (P<0.05). It is concluded that enzymatically treated Artemisia annua has the potential of antibiotic substitutes in broiler diets. The addition of enzymatically treated Artemisia annua to the diet alone has a tendency to improve the performance of broilers. Enzymatically treated Artemisia annua combined with Bacillus licheniformis can improve the growth performance of broilers by improving the structure of cecal microbiota, increasing the richness and diversity of intestinal microbiota, and increasing the relative abundance ratio of Firmicutes to Bacteroides.

参考文献

[1] 徐杰,邓龙兴,胡国元,等.青蒿素衍生物抗菌机理研究[J].天然产物研究与开发,2018,30(5):725-730. XU J,DENG L X,HU G Y,et al.Study on antibacterial mechanism of artemisinin derivatives[J].Natural Product Research and Development,2018,30(5):725-730.(in Chinese)
[2] MAGBOOL F A R,HUSSEIN S E O.Pharmacological aspect of artemisinin and aresunate as potent antimalarial agents-overview[J].European Journal of Pharmaceutical and Medical Research,2018,5(2):101-108.
[3] PURI M,SHARMA D,BARROW C J,et al.Enzyme-assisted extraction of bioactives from plants[J].Trends in Biotechnology,2012,30(1):37-44.  
[4] WAN X L,JIANG L Y,ZHONG H R,et al.Effects of enzymatically treated Artemisia annua L. on growth performance and some blood parameters of broilers exposed to heat stress[J].Animal Science Journal,2017,88(8):1239-1246.  
[5] PANAITE T D,CRISTE R D,VLAICU P A,et al.Influence of Artemisia annua on broiler performance and intestinal microflora[J].Brazilian Journal of Poultry Science,2019,21(4):1-9.
[6] CHEN Y C,YU Y H.Bacillus licheniformis-fermented products improve growth performance and the fecal microbiota community in broilers[J].Poultry Science,2020,99(3):1432-1443.  
[7] DARAFSH F,SOLTANI M,ABDOLHAY H A,et al.Improvement of growth performance,digestive enzymes and body composition of Persian sturgeon (Acipenser persicus) following feeding on probiotics:Bacillus licheniformis,Bacillus subtilis and Saccharomyces cerevisiae[J].Aquaculture Research,2020,51(3):957-964.  
[8] MIDHUN S J,NEETHU S,ARUN D,et al.Dietary supplementation of Bacillus licheniformis HGA8B improves growth parameters,enzymatic profile and gene expression of Oreochromis niloticus[J].Aquaculture,2019,505:289-296.
[9] SARACILA M,CRISTE R D,PANAITE T D,et al.Artemisia annua as phytogenic feed additive in the diet of broilers (14-35 days) reared under heat stress (32℃)[J].Brazilian Journal of Poultry Science,2018,20(4):825-832.  
[10] SONG Z H,CHENG K,ZHENG X C,et al.Effects of dietary supplementation with enzymatically treated Artemisia annua on growth performance,intestinal morphology,digestive enzyme activities,immunity,and antioxidant capacity of heat-stressed broilers[J].Poultry Science,2018,97(2):430-437.  
[11] 周梦佳,曾东,倪学勤,等.地衣芽孢杆菌H2对坏死性肠炎肉鸡生长性能及肠道菌群的影响[J].中国农业大学学报,2017,22(1):55-61. ZHOU M J,ZENG D,NI X Q,et al.Effects of Bacillus licheniformis H2 on the growth performance and gut microflora of broiler chickens infected with necrotic enteritis[J].Journal of China Agricultural University,2017,22(1):55-61.(in Chinese)
[12] 张金灵,李琰,殷明郁,等.几种多肽类抗生素添加剂在饲料中的应用研究[J].四川畜牧兽医,2014,41(2):37-38,41. ZANG J L,LI Y,YIN M Y,et al.Study on the application of antimicrobial peptide feed additives in feedstuff[J].Sichuan Animal & Veterinary Sciences,2014,41(2):37-38,41.(in Chinese)
[13] WAN X L,SONG Z H,NIU Y,et al.Evaluation of enzymatically treated Artemisia annua L. on growth performance,meat quality,and oxidative stability of breast and thigh muscles in broilers[J].Poultry Science,2017,96(4):844-850.  
[14] KIM H B,ISAACSON R E.The pig gut microbial diversity:understanding the pig gut microbial ecology through the next generation high throughput sequencing[J].Veterinary Microbiology,2015,177(3/4):242-251.
[15] KONSTANTINOV S R,FAVIER C F,ZHU W Y,et al.Microbial diversity studies of the porcine gastrointestinal ecosystem during weaning transition[J].Animal Research,2004,53(4):317-324.  
[16] 余怡然.青蒿鳖甲对MRL/lpr狼疮小鼠肠道微生物的影响研究[D].硕士学位论文.杭州:浙江中医药大学,2019:12-13. YU Y R.Effects of Artemisia annua turtle carapace on intestinal microorganisms in MRL/lpr lupus mice[D].Master's Thesis.Zhejiang:Zhejiang Chinese Medical University,2019:12-13.(in Chinese)
[17] 袁慧坤.日粮中添加芽孢杆菌对北京鸭生长性能、肠道菌群的影响[D].硕士学位论文.哈尔滨:东北农业大学,2019:28-31. YUAN H K.Effects of dietary supplementation of Bacillus on growth performance and intestinal flora in Beijing duck[D].Master's Thesis.Harbin:Northeast Agricultural University,2019:28-31.(in Chinese)
[18] 文秋,杨瑞瑞,金晓露.植物多酚对畜禽肠道健康的保护作用研究进展[J].中国科学(生命科学),2020,50(9):914-926. WEN Q,YANG R R,JIN X L.Research advances on the beneficial effects of plant polyphenols on intestinal health in farm animals[J].Science China(Life Sciences),2020,50(9):914-926.(in Chinese)
[19] YANG J J,ZHAN K,ZHANG M H.Effects of the use of a combination of two Bacillus species on performance,egg quality,small intestinal mucosal morphology,and cecal microbiota profile in aging laying hens[J].Probiotics and Antimicrobial Proteins,2020,12(1):204-213.  
[20] 黄梅,罗俊,沈建英.双氢青蒿素与头孢呋辛对大肠杆菌的协同抗菌作用及机制研究[J].中国中药杂志,2020,45(12):2975-2981. HUANG M,LUO J,SHEN J Y.Synergistic antibacterial effect and mechanisms of dihydroartemisinin and cefuroxime incombination[J].China Journal of Chinese Materia Medica,2020,45(12):2975-2981.(in Chinese)
[21] BUTAYE P,DEVRIESE L A,HAESEBROUCK F.Antimicrobial growth promoters used in animal feed:effects of less well known antibiotics on gram-positive bacteria[J].Clinical Microbiology Reviews,2003,16(2):175-188.  
[22] SHOAIE S,KARLSSON F,MARDINOGLU A,et al.Understanding the interactions between bacteria in the human gut through metabolic modeling[J].Scientific Reports,2013,3:2532.
[23] 韩岗,马婧,陶士珩,等.厚壁菌门细菌及其祖先蛋白的氨基酸偏好性研究[J].安徽农业科学,2011,39(30):18408-18410. HAN G,MA J,TAO S H,et al.The study of Amino acid biases in Firmicutes and their ancestral proteome[J].Journal of Anhui Agricultural Sciences,2011,39(30):18408-18410.(in Chinese)
[24] BRULC J M,ANTONOPOULOS D A,BERG MILLER M E,et al.Gene-centric metagenomics of the fiber-adherent bovine rumen microbiome reveals forage specific glycoside hydrolases[J].Proceedings of the National Academy of Sciences of the United States of America,2009,106(6):1948-1953.  
[25] ZHANG J C,GUO Z,XUE Z S,et al.A phylo-functional core of gut microbiota in healthy young Chinese cohorts across lifestyles,geography and ethnicities[J].The ISME Journal,2015,9(9):1979-1990.  
[26] 张亚楠,魏单平,韩瑞丽,等.高产期不同产蛋水平蛋鸡肠道微生物群落特征[J].中国兽医学报,2017,37(6):1179-1185. ZHANG Y N,WEI D P,HAN R L,et al.The character of the intestinal microbiota associated with laying performance in hens[J].Chinese Journal of Veterinary Science,2017,37(6):1179-1185.(in Chinese)
[27] TURNBAUGH P J,LEY R E,MAHOWALD M A,et al.An obesity-associated gut microbiome with increased capacity for energy harvest[J].Nature,2006,444(7122):1027-1031.  
[28] ZHANG W,MA C,XIE P,et al.Gut microbiota of newborn piglets with intrauterine growth restriction have lower diversity and different taxonomic abundances[J].Journal of Applied Microbiology,2019,127(2):354-369.  
[29] 李亚丹,任宏伟,吴彦彬,等.拟杆菌与肠道微生态[J].微生物学通报,2008,35(2):281-285. LI Y D,REN H W,WU Y B,et al.Bacteroides and gut microbial ecology[J].Microbiology China,2008,35(2):281-285.(in Chinese)
[30] MIQUEL S,LECLERC M,MARTIN R,et al.Identification of metabolic signatures linked to anti-inflammatory effects of Faecalibacterium prausnitzii[J].mBio,2015,6(2):1-10.
[31] QUÉVRAIN E,MAUBERT M A,MICHON C,et al.Identification of an anti-inflammatory protein from Faecalibacterium prausnitzii,a commensal bacterium deficient in Crohn's disease[J].Gut,2016,65(3):425-425.
[32] SUNKARA L T,ACHANTA M,SCHREIBER N B,et al.Butyrate enhances disease resistance of chickens by inducing antimicrobial host defense peptide Gene expression[J].PLoS One,2011,6(11):e27225.
[33] 卢烽,廖小军,胡小松,等.多酚对肠道微生物影响的研究进展及对多酚指示菌的探讨[J].食品工业科技,2018,39(16):330-335. LU F,LIAO X J,HU X S,et al.Research advances in the effect of polyphenols on the gut microbes and the discuss about microbes which will appear as polyphenols[J].Science and Technology of Food Industry,2018,39(16):330-335.(in Chinese)
文章导航

/