禽营养与饲料 POULTRY NUTRITION AND FEED

饲粮精氨酸水平对91~120日龄清远麻鸡生长性能、抗氧化能力、免疫功能和肉品质的影响

  • 范秋丽 ,
  • 叶金玲 ,
  • 林楚晓 ,
  • 林厦菁 ,
  • 李龙 ,
  • 邝智祥 ,
  • 国春艳 ,
  • 邓远帆 ,
  • 蒋守群 ,
  • 阮栋
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  • 1. 广东省农业科学院动物科学研究所, 畜禽育种国家重点实验室, 农业农村部华南动物营养与饲料重点实验室, 广东省畜禽育种与营养研究重点实验室, 广州 510640;
    2. 广东爱健康生物科技有限公司, 广州 511500;
    3. 希杰(上海)商贸有限公司, 上海 201107
范秋丽(1987-),女,陕西渭南人,助理研究员,硕士,从事黄羽肉鸡营养与饲料研究。E-mail:649698130@qq.com

收稿日期: 2020-12-04

  网络出版日期: 2021-07-06

基金资助

国家重点研发计划(2018YFD0500600);国家肉鸡产业技术体系项目(CARS-41-G10);广东省自然科学基金项目(2019A1515010912);广东省科技计划项目(2019A050505007);广州市科技计划重点项目(201804020091);广东省农业科学院科技计划项目(201601TD);科技创新战略专项资金(高水平农科院建设R2019PY-QF008)

Effects of Dietary Arginine Level on Growth Performance, Antioxidant Capacity, Immune Function and Meat Quality of Qingyuan Partridge Chickens during 91 to 120 Days of Age

  • FAN Qiuli ,
  • YE Jinling ,
  • LIN Chuxiao ,
  • LIN Xiajing ,
  • LI Long ,
  • KUANG Zhixiang ,
  • GUO Chunyan ,
  • DENG Yuanfan ,
  • JIANG Shouqun ,
  • RUAN Dong
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  • 1. Guangdong Key Laboratory of Animal Breeding and Nutrition, Key Laboratory of Animal Nutrition and Feed Science in South China, Ministry of Agriculture and Rural Affairs, State Key Laboratory of Livestock and Poultry Breeding, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China;
    2. Guangdong Aijiankang Biotechnology Co., Ltd., Guangzhou 511500, China;
    3. CJ (Shanghai) International Trading Co., Ltd., Shanghai 201107, China

Received date: 2020-12-04

  Online published: 2021-07-06

Supported by

 

摘要

本试验旨在研究饲粮精氨酸水平对91~120日龄清远麻鸡生长性能、抗氧化能力、免疫功能和肉品质的影响。试验选用1 050只91日龄清远麻鸡母鸡,采用完全随机分组设计分为5个组,每组6个重复,每个重复35只。各组饲粮精氨酸水平分别为0.66%、0.78%、0.90%、1.02%、1.14%。试验期30 d。结果表明:1)饲粮精氨酸水平对91~120日龄清远麻鸡平均日采食量、平均日增重和料重比均无显著影响(P>0.05)。2)随着饲粮精氨酸水平的升高,血浆和空肠黏膜总超氧化物歧化酶(T-SOD)活性、血浆总抗氧化力(T-AOC)呈线性和二次曲线升高(P<0.05),血浆丙二醛(MDA)含量呈线性和二次曲线降低(P<0.05),空肠谷胱甘肽过氧化酶1(GPX1)mRNA表达量呈线性和二次曲线升高(P<0.05)。3)饲粮精氨酸水平对120日龄清远麻鸡血浆免疫球蛋白A、免疫球蛋白G、免疫球蛋白M含量和空肠黏膜分泌型免疫球蛋白A(SIgA)、白细胞介素-2、白细胞介素-6含量均无显著影响(P>0.05),但空肠Toll样受体4(TLR4)和髓样分化因子88(MYD88)mRNA表达量随饲粮精氨酸水平的升高呈二次曲线降低(P<0.05),空肠闭合小环蛋白-1(ZO-1)mRNA表达量随饲粮精氨酸水平的升高呈二次曲线升高(P<0.05)。4)120日龄清远麻鸡屠宰率随饲粮精氨酸水平的升高呈线性和二次曲线增加(P<0.05),但饲粮精氨酸水平对胸肌剪切力、pH 和肉色均无显著影响(P>0.05)。综上所述,饲粮添加精氨酸可改善91~120日龄清远麻鸡肠道抗氧化能力和胴体性状,但对免疫功能和肉品质无明显影响。以平均日增重和料重比为主要评价指标,依据二次曲线模型估测,91~120日龄清远麻鸡饲粮精氨酸适宜水平分别为0.88%和0.94%。

本文引用格式

范秋丽 , 叶金玲 , 林楚晓 , 林厦菁 , 李龙 , 邝智祥 , 国春艳 , 邓远帆 , 蒋守群 , 阮栋 . 饲粮精氨酸水平对91~120日龄清远麻鸡生长性能、抗氧化能力、免疫功能和肉品质的影响[J]. 动物营养学报, 2021 , 33(7) : 3821 -3832 . DOI: 10.3969/j.issn.1006-267x.2021.07.024

Abstract

The purpose of this study was to investigate the effects of dietary arginine level on growth performance, antioxidant capacity, immune function and meat quality of Qingyuan partridge chickens during 91 to 120 days of age. One thousand and fifty 91-day-old female Qingyuan partridge female chickens were randomly assigned to 5 groups with 6 replicates per group and 35 chickens per replicate. The dietary arginine levels of each group were 0.66%, 0.78%, 0.90%, 1.02% and 1.14%, respectively. The experiment lasted for 30 days. The results showed as follows:1) dietary arginine level had no significant effects on the average daily feed intake, average daily gain and feed to gain ratio of Qingyuan partridge chickens during 91 to 120 days of age (P>0.05). 2) With dietary arginine level increasing, the activity of total superoxide dismutase (T-SOD) in plasma and jejunal mucosa and total antioxidant capacity (T-AOC) in plasma were increased linearly and quadratically (P<0.05), the plasma malondialdehyde (MDA) content was decreased linearly and quadratically (P<0.05), and the jejunal glutathione peroxidase 1 (GPX1) mRNA expression level was increased linearly and quadratically (P<0.05). 3) Dietary arginine level had no significant effects on the contents of immunoglobulin A, immunoglobulin G and immunoglobulin M in plasma and the contents of secretion immunoglobulin A, interleukin-2 and interleukin-6 in jejunal mucosa of Qingyuan partridge chickens at 120 days of age (P>0.05), but the mRNA expression levels of Toll-like receptor 4 (TLR4) and myeloid differentiation factor 88 (MYD88) in jejunum were decreased quadratically with dietary arginine level increasing (P<0.05), and the mRNA expression level of zonula occludens protein-1 (ZO-1) in jejunum was increased quadratically with dietary arginine level increasing (P<0.05). 4) The dressing rate of Qingyuan partridge chickens at 120 days of age was increased linearly and quadratically with dietary arginine level increasing (P<0.05), but dietary arginine level had no significant effects on shear force, pH and meat color in breast (P>0.05). In summary, dietary supplemented with arginine can improve intestinal antioxidant capacity and carcass traits of Qingyuan partridge chickens during 91 to 120 days of age, but have no significant effects on immune function and meat quality. Based on average daily gain and feed to gain ratio, the optimal arginine levels estimated by regression analysis for Qingyuan partridge chickens during 91 to 120 days of age are 0.88% and 0.94%, respectively.

参考文献

[1] 代张超,李吕木,卫爱莲,等.畜禽饲用精氨酸的研究进展[J].中国饲料,2018(13):5-9. DAI Z C,LI L M,WEI A L,et al.Research advances of arginine for livestock and poultry[J].China Feed,2018(13):5-9.(in Chinese)
[2] 李建慧,苗志强,车向荣,等.精氨酸对畜禽营养调控及肠道屏障功能的影响研究进展[J].中国畜牧兽医,2016,43(2):407-412. LI J H,MIAO Z Q,CHE X R,et al.Research advanced on functions of nutritional regulation and intestinal barrier of arginine[J].China Animal Husbandry & Veterinary Medicine,2016,43(2):407-412.(in Chinese)
[3] GAO K G,JIANG Z Y,LIN Y C,et al.Dietary L-arginine supplementation enhances placental growth and reproductive performance in sows[J].Amino Acids,2012,46(2):2207-2214.
[4] 杨书慧,谭灵琳,周建,等.L-精氨酸对热应激肉鸡肌肉品质和抗氧化功能及能量代谢的影响[J].中国畜牧杂志,2014,50(19):37-41. YANG S H,TAN L L,ZHOU J,et al.Effects of L-arginine on meat quality,antioxidative function,and energy metabolism of broilers under heat stress[J].Chinese Journal of Animal Science,2014,50(19):37-41.(in Chinese)
[5] EMAMI N K,GOLIAN A,RHOADS D D,et al.Interactive effects of temperature and dietary supplementation of arginine or guanidinoacetic acid on nutritional and physiological responses in male broiler chickens[J].British Poultry Science,2017,58(1):87-94.  
[6] ZHU H L,LIU Y L,XIE X L,et al.Effect of L-arginine on intestinal mucosal immune barrier function in weaned pigs after Escherichia coli LPS challenge[J].Innate Immunity,2013,19(3):242-252.  
[7] FATHI M,ADI K N,NEZHAD Y E,et al.The effects of vitamin E and L-arginine supplementation on antioxidant status and biochemical indices of broiler chickens with pulmonary hypertension syndrome[J].Journal of Animal and Veterinary Advances,2012,11(2):158-164.  
[8] 中华人民共和国农业部.NY/T 33-2004鸡饲养标准[S].北京:中国农业出版社,2004. Ministry of Agriculture of the People's Republic of China.NY/T 33-2004 Chicken feeding standard[S].Beijing:China Standard Press,2004.(in Chinese)
[9] 中国饲料数据库.中国饲料成分及营养价值表(2018年第29版)[J].中国饲料,2018(21):67-76. China Feed Database.Notes on the formulation of Chinese feed composition and nutritional value table (29th edition,2018)[J].China Feed,2018(21):67-76.(in Chinese)
[10] 范秋丽,蒋守群,苟钟勇,等.枯草芽孢杆菌、低聚壳聚糖和丁酸钠对黄羽肉鸡生长性能、免疫功能和肉品质的影响[J].中国畜牧兽医,2020,47(4):1080-1091. FAN Q L,JIANG S Q,GOU Z Y,et al.Effects of Bacillus subtilis,oligomeric chitosan and sodium butyrate on growth performance,immune function and meat quality in yellow-feathered chickens[J].Chinese Animal Husbandry and Veterinary,2020,47(4):1080-1091.(in Chinese)
[11] 范秋丽,李辉,蒋守群,等.姜辣素和大蒜素及其组合对817肉鸡生长性能、抗氧化和免疫功能的影响[J].动物营养学报,2020,32(9):4132-4139. FAN Q L,LI H,JIANG S Q,et al.Effects of gingerol,allicin and their combination on growth performance,antioxidant and immune function of 817 broilers[J].Chinese Journal of Animal Nutrition,2018,30(12):146-160.(in Chinese)
[12] 闻治国,吴学壮,齐志国,等.橡胶籽油对脂多糖刺激蛋鸡产蛋性能、肠道形态和免疫功能的影响[J].中国畜牧杂志,2020,56(12):145-152. WEN Z G,WU X Z,QI Z G,et al.Effects of rubber seed oil on egg production performance intestinal morphology and immune function of laying hens stimulated by polysaccharide[J].Chinese Journal of Animal Science,2020,56(12):145-152.(in Chinese)
[13] BERRES J,VIEIRA S L,FAVERO A,et al.Digestible valine requirements in high protein diets for broilers from twenty-one to forty-two days of age[J].Animal Feed Science and Technology,2011,165(1/2):120-124.
[14] 陈西风,刘维平.饲粮中补充不同水平精氨酸对肉鸡生长性能及免疫的影响[J].中国饲料,2019(21):51-53. CHEN X F,LIU W P.Effects of dietary arginine supplementation on growth performance and immune status of broiler chickens[J].China Feed,2019(21):51-53.(in Chinese)
[15] 郭祎玮.精氨酸对肉仔鸡生长性能和免疫功能的影响及其机理研究[D].博士学位论文.呼和浩特:内蒙古农业大学,2014:15-25. GUO Y W.Effect of arginine on growth performance and immune function of broilers and its mechanism[D].Ph.D.Thesis.Hohhot:Inner Mongolia Agricultural University,2014:15-25.(in Chinese)
[16] EMADI M,KAVEH K,BIN BEJO M H,et al.Growth performance and blood parameters as influenced by different levels of dietary arginine in broiler chickens[J].Journal of Animal and Veterinary Advances,2012,9(1):70-74.
[17] 朱伟,姜威,李新杰,等.精氨酸对夏季旱养肉鸭生长性能和免疫器官的影响[J].粮食与饲料工业,2013(1):49-52. ZHU W,JIANG W,LI X J,et al.Effects of dietary arginine on growth performance and immune organs of dry-feeding meat ducks in summer[J].Cereal & Feed Industry,2013(1):49-52.(in Chinese)
[18] 晏利琼,廖满,谢佳倩,等.L-精氨酸和α-酮戊二酸对热应激肉鸡肠道吸收功能、抗氧化能力、能量代谢的影响[J].中国畜牧杂志,2016,52(15):33-41. YAN L Q,LIAO M,XIE J Q,et al.Effects of L-arginine and α-ketoglutaric acid on intestinal absorption,antioxidant capacity,energy metabolism of heat-stressed broilers[J].Chinese Journal of Animal Science,2016,52(15):33-41.(in Chinese)
[19] RUAN D,Zhu Y W,FOUAD A M,et al.Dietary curcumin enhances intestinal antioxidant capacity in ducklings via altering gene expression of antioxidant and key detoxification enzymes[J].Poultry Science,2019,98(5):3705-3714.
[20] BIRMANI M W,RAZA A,NAWAB A,et al.Importance of arginine as immune regulator in animal nutrition[J].International Journal of Veterinary Sciences Research,2019,5(1):1-10.  
[21] PEREZ-CARBAJAL C,CALDWELL D,FARNELL M,et al.Immune response of broiler chickens fed different levels of arginine and vitamin E to a coccidiosis vaccine and Eimeria challenge[J].Poultry Science,2010,89(9):1870-1877.  
[22] 张灿菲.精氨酸对急性热应激鸡肠道黏膜免疫的影响[D].硕士学位论文.武汉:华中农业大学,2008. ZHANG C F.Effects of arginine on intestinal mucosal immunity of acute heat stress chickens[D].Master's Thesis.Wuhan:Huazhong Agricultural University,2008.(in Chinese)
[23] 吴天佑.精氨酸对脂多糖诱发泌乳奶牛炎性反应的缓解作用及其分子机制[D].硕士学位论文.扬州:扬州大学,2016:5-25. WU T Y.Effect of arginine on inflammatory response induced by lipopolysaccharide in lactating dairy cows and its molecular mechanism[D].Master's Thesis.Yangzhou:Yangzhou University,2016:5-25.(in Chinese)
[24] 彭众.早期断奶对树突状细胞介导的仔猪肠道黏膜免疫功能的影响及精氨酸对其的调节作用[D].硕士学位论文.扬州:扬州大学,2019:10-18. PENG Z.Effect of early weaning on intestinal mucosal immunity mediated by dendritic cells and regulation by arginine in piglets[D] Master's Thesis.Yangzhou:Yangzhou University,2019:10-18.(in Chinese)
[25] CHEN Y,CHEN D W,TIAN G,et al.Dietary arginine supplementation alleviates immune challenge induced by Salmonella enterica serovar Choleraesuis bacterin potentially through the Toll-like receptor 4-myeloid differentiation factor 88 signalling pathway in weaned piglets[J].British Journal of Nutrition,2012,108(6):1069-1076.  
[26] 赵剑,孙得发,边四辈,等.轻度氧化油脂对肉仔鸡生长性能、脏器指数和肠道形态的影响[J].中国畜牧杂志,2018,54(2):76-79. ZHAO J,SUN D F,BIAN S B,et al.Effects of slightly oxidized fat on growth performance viscera index and intestinal morphology of broilers[J].Chinese Journal of Animal Science,2018,54(2):76-79.(in Chinese)
[27] TAHMASEBI S,TOGHYANI M.Effect of arginine and threonine administered in ovo on digestive organ developments and subsequent growth performance of broiler chickens[J].Journal of Animal Physiology and Animal Nutrition,2016,100(5):947-956.  
[28] VAN ITALLIE C M,TIETGENS A J,ANDERSON J M.Visualizing the dynamic coupling of claudin strands to the actin cytoskeleton through ZO-1[J].Molecular Biology of the Cell,2017,28(4):524-534.  
[29] XIA Z F,HUANG L Q,PENG Y,et al.L-arginine alleviates heat stress-induced intestinal epithelial barrier damage by promoting expression of tight junction proteins via the AMPK pathway[J].Molecular Biology Reports,2019,46(6):6435-6451.  
[30] 张贝贝.精氨酸对肉仔鸡肠道黏膜损伤的缓解作用及机理研究[D].博士学位论文.北京:中国农业大学,2018:5-35. ZHANG B B.Study on the effect and mechanism of arginine on intestinal mucosal injury of broilers[D].Ph.D.Thesis.Beijing:China Agricultural University,2018:5-35.(in Chinese)
[31] SIEKMANN L,MEIER-DINKEL L,JANISCH S,et al.Carcass quality,meat quality and sensory properties of the dual-purpose chicken lohmann dual[J].Foods,2018,7(10):156.
[32] 朗倩倩,张燕,徐振强,等.黄羽肉鸡体尺性状与腹脂沉积相关性的研究[J].中国畜牧杂志,2020,56(3):43-46. LANG Q Q,ZHANG Y,XU Z Q,et al.Study on the Correlation between body size and ventral fat deposition in Yellow feather broiler chickens[J].Chinese Journal of Animal Science,2020,56(3):43-46.(in Chinese)
[33] AI-DARAJI H J,SALIH A M.Effect of dietary L-arginine on carcass traits of broilers[J].Research Opinions in Animal & Veterinary Sciences,2012,2(2):40-44.
[34] WU L Y,FANG Y J,GUO X Y.Dietary L-arginine supplementation beneficially regulates body fat deposition of meat-type ducks[J].British Poultry Science,2011,52(2):221-226.  
[35] YU M,LI Z M,RONG T,et al.Different dietary starch sources alter the carcass traits,meat quality,and the profile of muscle amino acid and fatty acid in finishing pigs[J].Journal of Animal Science and Biotechnology,2020,11:78.
[36] 陈婷,廉俊红,吴佳韩,等.复合益生菌发酵饲料对雪峰乌骨鸡日粮表观消化率及肉品质的影响[J].中国畜牧兽医,2019,46(10):2964-2972. CHEN T,LIAN J H,WU J H,et al.Effect of compound probiotics fermentation feed on apparent digestibility and meat quality of Xuefeng black chicken diet[J].China Animal Husbandry & Veterinary Medicine,2019,46(10):2964-2972.(in Chinese)
[37] 施展,单安山.妊娠期母猪DDGS型饲粮添加精氨酸和铬对子代肉品质的影响[C]//中国畜牧兽医学会动物营养学分会第七届中国饲料营养学术研讨会.郑州:国农业大学出版社,2014. SHI Z,SHAN A S.Effects of DDGS diet with arginine and chromium on the quality of offspring meat in pregnant sows[C]//Symposium on feed nutrition in China,Animal Nutrition Branch of Chinese Society of Animal Husbandry and Veterinary. Zhengzhou:China Agricultural University Press,2014.(in Chinese)
[38] 李燕舞,石英,庞纪彩.L-精氨酸对肥育猪生长性能、营养物质消化率、气体排放和肉质的影响[J].中国饲料,2019(18):76-79. LI Y W,SHI Y,PANG J C.Effects of L-arginine on growth performance,nutrient digestibility,gas emission and meat quality of finishing pigs[J].China Feed,2019(18):76-79.(in Chinese)
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