禽营养与饲料 POULTRY NUTRITION AND FEED

不同养殖方式下饲粮中添加丁酸梭菌对广西黄鸡生长性能、肠道形态与盲肠微生物区系的影响

  • 韦晓芳 ,
  • 沈水宝 ,
  • 吴克宁 ,
  • 李修宇 ,
  • 袁汝喜 ,
  • 雷丽莉 ,
  • 张淑芳 ,
  • 章岳军
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  • 广西大学动物科学技术学院, 南宁 530004
韦晓芳(1996-),女,广西河池人,硕士研究生,研究方向为生物发酵饲料与现代生态养殖。E-mail:2627463483@qq.com

收稿日期: 2020-10-31

  网络出版日期: 2021-07-06

基金资助

“畜禽重大疫病防控与高效安全养殖综合技术研发”重点专项——畜禽肠道健康调控的生物饲料生产与饲喂技术研究(2018YFD0500603)

Effects of Adding Clostridium butyricum to Diet under Different Raising Modes on Growth Performance, Intestinal Morphology and Cecal Microflora of Guangxi Yellow Chickens

  • WEI Xiaofang ,
  • SHEN Shuibao ,
  • WU Kening ,
  • LI Xiuyu ,
  • YUAN Ruxi ,
  • LEI Lili ,
  • ZHANG Shufang ,
  • ZHANG Yuejun
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  • School of Animal Science and Technology, Guangxi University, Nanning 530004, China

Received date: 2020-10-31

  Online published: 2021-07-06

Supported by

 

摘要

本试验旨在研究不同养殖方式下饲粮中添加丁酸梭菌对广西黄鸡生长性能、肠道形态与盲肠微生物区系的影响。试验选取35日龄健康状态良好的广西黄鸡3 300羽,随机分为2个组,每组5个重复,每个重复330羽。2个组分别为对照组(基础饲粮)和试验组(基础饲粮+500 g/t丁酸梭菌,制粒后活菌数≥2.88×105 CFU/g),分2个阶段进行饲养试验:第1阶段35~49日龄,笼养方式;第2阶段50~80日龄,转换为地面散养方式。结果表明:1)35~49日龄笼养阶段,饲粮中添加丁酸梭菌对广西黄鸡的生长性能影响不大,但具有提高广西黄鸡平均日增重以及降低料重比的趋势(P>0.05)。2)35~49日龄笼养阶段和50~80日龄散养阶段,饲粮中添加丁酸梭菌显著提高十二指肠、空肠、回肠的绒毛高度(P<0.05),显著降低隐窝深度(P<0.05),显著提高绒毛高度/隐窝深度(P<0.05)。3)在不同的养殖模式下,饲粮中添加丁酸梭菌可显著提高厚壁菌门相对丰度(P<0.05),有提升梭菌属和瘤胃球菌属相对丰度的趋势(P>0.05);且笼养转散养后,拟杆菌门的相对丰度显著提高(P<0.05),盲肠菌群的相对丰度增加(P>0.05)。综上所述,在不同养殖方式下,饲粮中添加丁酸梭菌对不同日龄广西黄鸡的生长性能未产生显著影响,但有提高平均日增重以及降低料重比的趋势,可以促进广西黄鸡肠道形态的发育,提高盲肠菌群的相对丰度,且笼养转散养后,盲肠菌群的相对丰度可以得到进一步的提升。

本文引用格式

韦晓芳 , 沈水宝 , 吴克宁 , 李修宇 , 袁汝喜 , 雷丽莉 , 张淑芳 , 章岳军 . 不同养殖方式下饲粮中添加丁酸梭菌对广西黄鸡生长性能、肠道形态与盲肠微生物区系的影响[J]. 动物营养学报, 2021 , 33(7) : 3842 -3852 . DOI: 10.3969/j.issn.1006-267x.2021.07.026

Abstract

The purpose of this experiment was to study the effects of adding Clostridium butyricum to the diet under different raising modes on the growth performance, intestinal morphology and caecal microflora of Guangxi yellow chickens. The experiment selected 3 300 Guangxi yellow chickens with good health at 35 days of age and randomly divided them into 2 groups with 5 replicates in each group and 330 birds in each replicate. The two groups were the control group (basic diet) and the test group (basic diet + 500 g/t Clostridium butyricum, the number of viable cells after pelleting was ≥ 2.88×105 CFU/g), and the feeding experiment was carried out in 2 stages:the first stage was 35 to 49 days of age, caged. The second stage was 50 to 80 days of age, and it was converted to ground free-range breeding. The results showed as follows:1) in the 35 to 49-day-old caged-raising stage, dietary Clostridium butyricum had little effect on the growth performance of Guangxi yellow chickens, but it had a tendency to increase the average daily gain and decrease the ratio of feed to gain (P>0.05). 2) In the 35 to 49-day-old caged-raising stage and the 50 to 80-day-old free-raising stage, the addition of Clostridium butyricum to the diet significantly increased the villus height of the duodenum, jejunum and ileum (P<0.05), and significantly reduced crypt depth (P<0.05), significantly increased villus height/crypt depth (P<0.05). 3) Under different raising modes, adding Clostridium butyricum to the diet could significantly increase the relative abundance of Firmicutes (P<0.05), and had a tendency to increase the relative abundance of Clostridium and Rumenococcus (P>0.05); and after the cage transfer to free-raising, the relative abundance of Bacteroides increased significantly (P<0.05), and the relative abundance of cecal flora increased (P>0.05). In summary, under different raising modes, adding Clostridium butyricum to the diet have no significant impact on the growth performance of Guangxi yellow chickens at different ages, but there is a trend to increase the average daily gain and reduce the feed-to-weight ratio, promote the development of the intestinal morphology of Guangxi yellow chickens, and increase the abundance of the cecal flora, and the richness of the cecal flora can be further improved after the cage is converted to free-raising.

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