动物营养学报 >

2021 , Vol. 33 >Issue 7: 3885 - 3895

DOI: https://doi.org/10.3969/j.issn.1006-267x.2021.07.030

反刍与草食动物营养与饲料 RUMINANT AND HERBIVORE NUTRITION AND FEED

补饲过瘤胃蛋氨酸、甜菜碱及B族维生素复合物对围产期奶牛健康及生产性能的影响

  • 杜超 ,
  • 梁静 ,
  • 王翌翀 ,
  • 侯明杰 ,
  • 吴兆海 ,
  • 卜登攀 ,
  • 张文举
展开
  • 1. 石河子大学动物科技学院, 石河子 271018;
    2. 中国农业科学院北京畜牧兽医研究所, 动物营养学国家重点实验室, 北京 100193;
    3. 北京农学院动物科学技术学院, 北京 102206;
    4. 甘肃民族师范学院, 合作 747000
杜超(1991-),男,陕西周至人,博士研究生,从事反刍动物营养研究。E-mail:duchao105@163.com

收稿日期: 2020-12-16

  网络出版日期: 2021-07-06

基金资助

中国农业科学院科技创新工程（ASTIP-IAS07-1）；兵团重大科技计划项目（2021AA004）；中国农业科学院农业科技创新工程重大产出科研选题（CAAS-ZDXT2019004）；奶牛产业技术体系北京市创新团队（BAIC06-2021）

收起

Effects of Supplementary Feeding Rumen Bypass Methionine, Betaine and Vitamin B Complex on Health and Performance of Perinatal Dairy Cows

  • DU Chao ,
  • LIANG Jing ,
  • WANG Yichong ,
  • HOU Mingjie ,
  • WU Zhaohai ,
  • BU Dengpan ,
  • ZHANG Wenju
Expand
  • 1. College of Animal Science and Technology, Shihezi University, Shihezi 271018, China;
    2. State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China;
    3. College of Animal Science and Technology, Beijing University of Agriculture, Beijing 102206, China;
    4. Gansu Normal College for Nationalities, Hezuo 747000, China

Received date: 2020-12-16

  Online published: 2021-07-06

Supported by

 

Fold

摘要

本试验旨在研究在干奶期至新产期阶段补饲过瘤胃蛋氨酸、甜菜碱及B族维生素复合物对围产期奶牛健康及生产性能的影响。选取预产期前60 d的经产荷斯坦干奶牛48头，随机分为3组（每组16头牛）：对照组奶牛饲喂基础饲粮，试验组每头牛每天在饲粮中分别添加100和150 g过瘤胃蛋氨酸、甜菜碱和B族维生素复合物。试验自奶牛预产期前60 d开始，至产后30 d结束，共计90 d。结果显示：与对照组相比，补饲150 g/（头·d）过瘤胃蛋氨酸、甜菜碱和B族维生素复合物显著增加了产后30 d的产奶量（P=0.04），有增加产前30 d血清中白细胞介素-1含量的趋势（P=0.08），对血清中钙、磷及维生素B1和维生素B3含量无显著影响（P>0.05），对奶牛体况评分无显著影响（P>0.05），对奶牛妊娠时长和犊牛初生重亦无显著影响（P>0.05）。由此可见，在干奶期至新产期阶段补饲过瘤胃蛋氨酸、甜菜碱和B族维生素复合物能有效提升奶牛机体健康和产奶量，每头牛每天补饲150 g对奶牛产后生产性能较好。

关键词： 过瘤胃蛋氨酸; 甜菜碱; B族维生素; 围产期; 奶牛; 产奶量

本文引用格式

杜超 , 梁静 , 王翌翀 , 侯明杰 , 吴兆海 , 卜登攀 , 张文举 . 补饲过瘤胃蛋氨酸、甜菜碱及B族维生素复合物对围产期奶牛健康及生产性能的影响[J]. 动物营养学报, 2021 , 33(7) : 3885 -3895 . DOI: 10.3969/j.issn.1006-267x.2021.07.030

Abstract

This experiment aimed to study the effects of supplementary feeding rumen bypass methionine, betaine and vitamin B complex from dry period to fresh period on the health and performance of dairy cows. Forty-eight Holstein dry-off cows at 60 days before expected calving date were selected and randomly assigned to 3 groups with each group had 16 cows:dairy cows in control group were fed a basal diet, and those in experiment groups were fed the basal diet supplemented with 100 or 150 g rumen-protected methionine, betaine and vitamin B complex per head per day. The experiment started from 60 days before expected calving day and ended 30 days after calving, a total of 90 days. The results showed that supplementary feeding 150 g/(head·d) rumen-protected methionine, betaine and B vitamin complex significantly increased the milk yield at 30 days after calving (P=0.04), and had a tendency to increase the content of interleukin-1 (IL-1) in the serum at 30 days before calving compared with the control group (P=0.08). Supplementary feeding 150 g/(head·d) rumen-protected methionine, betaine and B vitamin complex had no significant effects on the contents of serum calcium, phosphorus, vitamin B1 and vitamin B3 (P>0.05), and had no significant effect on the body condition score of dairy cows (P>0.05). There was no significant effect on the pregnancy length of dairy cows and the birth weight of calves among groups (P>0.05). It is concluded that supplementary feeding rumen bypass methionine, betaine and vitamin B complex from dry period to fresh period can effectively increase the body health and milk yield of dairy cows, and supplementary feeding 150 g per cow per day is better for postpartum performance of dairy cows.

Key words： rumen bypass methionine; betaine; vitamin B; peripartum; dairy cows; milk yield

参考文献

[1] GRUMMER R R.Impact of changes in organic nutrient metabolism on feeding the transition dairy cow[J].Journal of Animal Science,1995,73(9):2820-2833.  
[2] DRACKLEY J K.ADSA Foundation Scholar Award.Biology of dairy cows during the transition period:the final frontier?[J].Journal of Dairy Science,1999,82(11):2259-2273.  
[3] LEWIS G S.Uterine health and disorders[J].Journal of Dairy Science,1997,80(5):984-994.  
[4] KIMURA K,REINHARDT T,GOFF J P.Parturition and hypocalcemia blunts calcium signals in immune cells of dairy cattle[J].Journal of Dairy Science,2006,89(7):2588-2595.  
[5] OSPINA P A,NYDAM D V,STOKOL T,et al.Association between the proportion of sampled transition cows with increased nonesterified fatty acids and beta-hydroxybutyrate and disease incidence,pregnancy rate,and milk production at the herd level[J].Journal of Dairy Science,2010,93(8):3595-3601.  
[6] OVERTON T R,WALDRON M R.Nutritional management of transition dairy cows:strategies to optimize metabolic health[J].Journal of Dairy Science,2004,87:105-119.
[7] ALMANNAI M,ALFADHEL M,EL-HATTAB A W.Carnitine inborn errors of metabolism[J].Molecules,2019,24(18):3251.
[8] CALÒ L A,VERTOLLI U,DAVIS P A,et al.L-carnitine in hemodialysis patients[J].Hemodialysis International. International Symposium on Home Hemodialysis,2012,16(3):428-434.  
[9] GRUMMER R R,MASHEK D G,HAYIRLI A.Dry matter intake and energy balance in the transition period[J].The Veterinary Clinics of North America:Food Animal Practice,2004,20(3):447-470.  
[10] GAGNON A,KHAN D R,SIRARD M,et al.Effects of intramuscular administration of folic acid and vitamin B12 on granulosa cells gene expression in postpartum dairy cows[J].Journal of Dairy Science,2015,98(11):7797-7809.  
[11] MORRISON E I,REINHARDT H,LECLERC H,et al.Effect of rumen-protected B vitamins and choline supplementation on health,production,and reproduction in transition dairy cows[J].Journal of Dairy Science,2018,101(10):9016-9027.  
[12] WANG C,LIU H,WANG C,et al.Effects of dietary rumen-protected betaine on lactation performance and serum metabolites of mid-lactation Holstein dairy cows[J].Journal of Agricultural and Food Chemistry,2020,68(46):13154-13159.  
[13] CHENG J B,WANG J Q,BU D P,et al.Factors affecting the lactoferrin concentration in bovine milk[J].Journal of Dairy Science,2008,91(3):970-976.  
[14] HARDER U,KOLETZKO B,PEISSNER W.Quantification of 22 plasma amino acids combining derivatization and ion-pair LC-MS/MS[J].Journal of Chromatography B:Analytical Technologies in the Biomedical and Life Sciences,2011,879(7/8):495-504.
[15] EDMONSON A J,LEAN I J,WEAVER L D,et al.A body condition scoring chart for Holstein dairy Cows[J].Journal of Dairy Science,1989,72(1):68-78.  
[16] 孙菲菲.胆碱和蛋氨酸对奶牛围产期营养平衡和机体健康的影响及机制[D].博士学位论文.杨凌:西北农林科技大学,2017. SUN F F.Choline and methionine regulate nutrient balance and health of transition dairy cows:responses and potential mechnisms[D].Ph.D.Thesis.Yangling:Northwest A&F University,2017.(in Chinese)
[17] AWAWDEH M S.Rumen-protected methionine and lysine:effects on milk production and plasma amino acids of dairy cows with reference to metabolisable protein status[J].The Journal of Dairy Research,2016,83(2):151-155.  
[18] VAILATI-RIBONI M,CROOKENDEN M,KAY JK,et al.Hepatic one-carbon metabolism enzyme activities and intermediate metabolites are altered by prepartum body condition score and plane of nutrition in grazing Holstein dairy cows[J].Journal of Dairy Science,2020,103(3):2662-2676.  
[19] MENCHU S.Ruminal protection and intestinal availability of rumen-protected methionine and lysine in lactating dairy cows[D].Master's Thesis.Logan:Utah State University,2019:7451.
[20] VAN DER KOLK J H H,GROSS J,GERBER V,et al.Disturbed bovine mitochondrial lipid metabolism:a review[J].Veterinary Quarterly,2017,37(1):262-273.  
[21] 田雨,杨榛,韩兆玉.包被甜菜碱对奶牛产奶性能及血液生化指标的影响[J].中国奶牛,2016(1):1-5. TIAN Y,YANG Z,HAN Z Y.Effects of rumen protected betaine on milk performance and blood parameters in dairy cows[J].China Dairy Cattle,2016(1):1-5.(in Chinese)
[22] DUNSHEA F R,OLUBOYEDE K,DIGIACOMO K,et al.Betaine improves milk yield in grazing dairy cows supplemented with concentrates at high temperatures[J].Animals,2019,9(2):57.
[23] WANG C H,LIU H,WANG C,et al.Effects of dietary rumen-protected betaine on lactation performance and serum metabolites of mid-lactation Holstein dairy cows[J].Journal of Agricultural and Food Chemistry,2020.68(46):13154-13159.
[24] EUN H M.Enzymes and nucleic acids:general principles,in enzymology primer for recombinant DNA technology[M].EUN H M.Enzymology primer for recombinant DNA technology.Pittsburgh:Academic Press,1996:1-108.
[25] SAID H M.Water-soluble vitamins:absorption,metabolism,and deficiency[M].JOHNSON L R.Encyclopedia of gastroenterology.Pittsburgh:Academic Press,2004:631-637.
[26] SHABBIR H.Fundamental aspects of vitamin B complex in human nourishment and fitness[J].American Journal of Food Science and Health,2020,6(4):109-118.
[27] LYON P,STRIPPOLI V,FANG B,et al.B vitamins and one-carbon metabolism:implications in human health and disease[J].Nutrients,2020,12(9):2867.
[28] 韩兆玉.过瘤胃蛋氨酸对奶牛夏季生产性能及淋巴细胞凋亡调控的影响[D].博士学位论文.南京:南京农业大学,2007. HAN Z Y.The effects of rumen protected methionine on performances and apoptotic regulation of lymphocyte of dairy cows in summer[D].Ph.D.Thesis.Nanjing:Naning Agricultural University,2007.(in Chinese)
[29] ZHANG X F,WANG Z,SHAH A M.Production performance,metabolic profile and calcium-regulating hormones of transition dairy cows with different blood calcium status after parturition[J].Pakistan Veterinary Journal,2020,40(1):19-24.
[30] 李远杰.围产期奶牛补饲N-氨甲酰谷氨酸对产奶性能、免疫及血液代谢物的影响[D].硕士学位论文.泰安:山东农业大学,2017. LI Y J.Effects of N-carbamylglutamate supplementation on milk performance blood metabolites and immunity in transition cows[D].Master's Thesis.Tai'an:Shandong Agricultural University,2017.(in Chinese)
[31] 王剑飞,王梦芝,冯春燕,等.过瘤胃蛋氨酸对奶牛瘤胃体外发酵及泌乳奶牛生产性能的影响[J].动物营养学报,2015,27(7):2248-2255. WANG J F,WANG M Z,FENG C Y,et al.Effects of rumen protected methionine on in vitro ruminal fermentation and performance of lactating dairy cows[J].Chinese Journal of Animal Nutrition,2015,27(7):2248-2255.(in Chinese)
[32] 刘飞,林雪彦,侯秋玲,等.瘤胃保护性蛋氨酸和赖氨酸补饲量对泌乳荷斯坦奶牛生产性能及血浆生化指标的影响[J].动物营养学报,2014,26(2):363-370. LIU F,LIN X Y,HOU Q L,et al.Effects of supplemental levels of rumen-protected methionine and lysine on performance and plasma biochemical indices of lactating Holstein cows[J].Chinese Journal of Animal Nutrition,2014,26(2):363-370.(in Chinese)
[33] RAJWADE N,KUMARI K,GENDLEY M K,et al.Effect of dietary supplementation of rumen protected methionine and lysine on haematological and blood biochemical profile in Sahiwal female calves[J].Bioinfo,2019,11(8):8322-8324.
[34] 张彩,田志刚.γ干扰素的负向免疫调节作用[J].现代免疫学,2007,27(2):89-92. ZHANG C,TIAN Z G.The negative immunomodulatory effect of gamma interferon[J].Current Immunology,2007,27(2):89-92.(in Chinese)
[35] VAILATI-RIBONI M,ZHOU Z,JACOMETO C B,et al.Supplementation with rumen-protected methionine or choline during the transition period influences whole-blood immune response in periparturient dairy cows[J].Journal of Dairy Science,2017,100(5):3958-3968.  
[36] LI C,WANG Y,LI L,et al.Betaine protects against heat exposure-induced oxidative stress and apoptosis in bovine mammary epithelial cells via regulation of ROS production[J].Cell Stress & Chaperones,2019,24(2):453-460.  
[37] PIRESTANI A,AGHAKHANI M.The effects of rumen-protected choline and L-carnitine supplementation in the transition period on reproduction,production,and some metabolic diseases of dairy cattle[J].Journal of Applied Animal Research,2018,46(1):435-440.  
[38] CHENG K F,WANG C,ZHANG G W,et al.Effects of betaine and rumen-protected folic acid supplementation on lactation performance,nutrient digestion,rumen fermentation and blood metabolites in dairy cows[J].Animal Feed Science and Technology,2020,262:114445.
[39] 王梦琦,朱小瑞,邢世宇,等.影响荷斯坦牛犊牛初生重的因素分析[J].家畜生态学报,2016,37(10):22-25. WANG M Q,ZHU X R,XING S Y,et al.Factors affecting the birth weight of Holstein calf[J].Acta Ecologae Animalis Domastici,2016,37(10):22-25.(in Chinese)
[40] MONTEIRO A P A,BERNARD J K,GUO J R,et al.Effects of feeding betaine-containing liquid supplement to transition dairy cows[J].Journal of Dairy Science,2017,100(2):1063-1071.  
Options
文章导航

/

〈 〉

    联系我们

  • 地址：北京市海淀区圆明园西路2号
    　　　中国农业大学动科动医大楼153室
  • 邮编：100193
  • 电话：010－62817823
  • 网址：http://www.chinajan.com
  • E-mail：cjan@chinajan.com
京公网安备 11010802041926号    京ICP备17050989号-2