动物营养学报 >

2021 , Vol. 33 >Issue 7: 3949 - 3958

DOI: https://doi.org/10.3969/j.issn.1006-267x.2021.07.036

反刍与草食动物营养与饲料 RUMINANT AND HERBIVORE NUTRITION AND FEED

包膜丁酸钠对断奶羔羊免疫和抗氧化指标的影响

  • 陈想 ,
  • 左丽君 ,
  • 王可鑫 ,
  • 贾斌 ,
  • 潘春媛 ,
  • 姜宁 ,
  • 张爱忠
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  • 1. 黑龙江八一农垦大学动物科技学院, 黑龙江省寒区饲料资源高效利用与营养调控重点实验室, 大庆 163319;
    2. 黑龙江省农业科学院畜牧兽医分院, 齐齐哈尔 161000
陈想(1996-),女,黑龙江双鸭山人,硕士研究生,研究方向为动物营养与饲料科学。E-mail:467231385@qq.com

收稿日期: 2020-12-16

  网络出版日期: 2021-07-06

基金资助

黑龙江省自然科学基金项目（LH2019C053）

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Effects of Coated Sodium Butyrate on Immune and Antioxidant Indexes of Weaned Lambs

  • CHEN Xiang ,
  • ZUO Lijun ,
  • WANG Kexin ,
  • JIA Bin ,
  • PAN Chunyuan ,
  • JIANG Ning ,
  • ZHANG Aizhong
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  • 1. Key Laboratory of Efficient Utilization of Feed Resources and Nutrition Control in Cold Regions of Heilongjiang Province, College of Animal Science and Technology, Heilongjiang Bayi Land Reclamation University, Daqing 163319, China;
    2. Animal Husbandry and Veterinary Branch of Heilongjiang Academy of Agricultural Sciences, Qiqihar 161000, China

Received date: 2020-12-16

  Online published: 2021-07-06

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摘要

本试验旨在研究包膜丁酸钠（CSB）对断奶羔羊免疫和抗氧化指标的影响。选择42日龄的断奶羔羊24只，随机分成4组，分别为空白对照组（CON组）、抗生素对照组（A CON组）和包膜丁酸钠A组（CSB A组）和包膜丁酸钠B组（CSB B组），每组6个重复，每个重复1只。CON组饲喂不含抗生素的基础饲粮，A CON组在基础饲粮中添加250 mg/kg的黄霉素，CSB A组和CSB B组在基础饲粮中分别添加2和3 g/kg包膜丁酸钠。预试期7 d，正试期28 d。各组分别在试验第14天和第28天选取3只羔羊屠宰取样。结果表明：在试验第14天和第28天，与CON组相比，饲粮中添加包膜丁酸钠对羔羊的肝脏、脾脏、胸腺指数均有所提高（P>0.05）。在试验第14天，与CON组相比，CSB A组与CSB B组血清中肿瘤坏死因子-α（TNF-α）和白细胞介素-6（IL-6）的含量显著下降（P<0.05），A CON组血清中白细胞介素-2（IL-2）、丙二醛（MDA）的含量显著下降（P<0.05）；与A CON组相比，CSB A组与CSB B组血清中IL-6的含量显著下降（P<0.05）。在试验第14天，与CON组相比，CSB B组肝脏中TNF-α、白细胞介素-1（IL-1）和IL-6的含量显著下降（P<0.05），CSB A组与CSB B组肝脏中IL-2的含量显著下降（P<0.05），CSB A组肝脏中总抗氧化能力（T-AOC）和总超氧化物歧化酶（T-SOD）的活性显著上升（P<0.05）。在试验第28天，与CON组相比，CSB A组血清中IL-6的含量显著下降（P<0.05），CSB A组与CSB B组血清中TNF-α的含量显著下降（P<0.05），而血清中T-SOD的活性显著上升（P<0.05）；与A CON组相比，CSB B组血清中TNF-α的含量显著下降（P<0.05），CSB A组与CSB B组血清中T-SOD的活性显著升高（P<0.05）。在试验第28天，与CON组相比，CSB A组与CSB B组肝脏中TNF-α、IL-1和IL-2的含量显著下降（P<0.05），而肝脏中T-AOC和T-SOD的活性显著上升（P<0.05）。由此可见，饲粮中添加包膜丁酸钠可以抑制断奶羔羊血清和肝脏中促炎因子含量的升高并增强其抗氧化能力，从而在一定程度上保护羔羊免受断奶应激带来的负面影响，其中2 g/kg的添加量较为适宜。

关键词： 包膜丁酸钠; 断奶羔羊; 免疫器官指数; 免疫指标; 抗氧化指标

本文引用格式

陈想 , 左丽君 , 王可鑫 , 贾斌 , 潘春媛 , 姜宁 , 张爱忠 . 包膜丁酸钠对断奶羔羊免疫和抗氧化指标的影响[J]. 动物营养学报, 2021 , 33(7) : 3949 -3958 . DOI: 10.3969/j.issn.1006-267x.2021.07.036

Abstract

This experiment was conducted to study the effects of coated sodium butyrate on the immune and antioxidant indexes of weaned lambs. Twenty-four 42-day-old weaned lambs were randomly divided into 4 groups:blank control group (CON group), antibiotic control group (A CON group), coated sodium butyrate A group (CSB A group) and coated sodium butyrate B group (CSB B group), with 6 replicates in each group. Lambs in the CON group were fed a basal diet without antibiotics, and those in the A CON group were fed the basal diet supplemented with 250 mg/kg flavomycin, while those in the CSB A group and CSB B group were fed the basal diet supplemented with 2 and 3 g/kg coated sodium butyrate, respectively. The pre-trial period lasted for 7 days, and the experiment lasted for 28 days. Three lambs of each group were slaughtered and sampled on the 14th and 28th day of the experiment. The result showed as follows:On the 14th and 28th day of the experiment, compared with the CON group, the liver, spleen and thymus indexes of lambs were increased when diet supplemented with coated sodium butyrate (P>0.05). On the 14th day of the experiment, compared with the CON group, the contents of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) in serum of CSB A group and CSB B group were significantly decreased (P<0.05), and the contents of interleukin-2 (IL-2) and malondialdehyde (MDA) in serum of A CON group were significantly decreased (P<0.05); compared with the A CON group, the IL-6 content in CSB A group and CSB B group was significantly decreased (P<0.05). On the 14th and 28th day of the experiment, compared with the CON group, the contents of TNF-α, interleukin-1 (IL-1) and IL-6 in liver were significantly decreased (P<0.05), and the IL-2 content in liver of CSB A group and CSB B group was significantly decreased (P<0.05), while the total antioxidant capacity (T-AOC) and total superoxide dismutase (T-SOD) activity in liver of CSB A group were significantly increased (P<0.05). On the 28th day of the experiment, compared with the CON group, the content of IL-6 in serum of CSB A group was significantly decreased (P<0.05), and the TNF-α content in serum of CSB A group and CSB B group was significantly decreased (P<0.05), while the T-SOD activity in serum of CSB A group and CSB B group was significantly increased (P<0.05); compared with the A CON group, the TNF-α content in serum of CSB B group was significantly decreased (P<0.05), while the T-SOD activity in serum of CSB A group and CSB B group was significantly increased (P<0.05). On the 28th day of the experiment, compared with the CON group, the contents of TNF-α, IL-1 and IL-2 in liver of CSB A group and CSB B group were significantly decreased (P<0.05), which the T-AOC and T-SOD activity in liver of CSB A group and CSB B group were significantly increased (P<0.05). Therefore, the addition of coated sodium butyrate in the diet can inhibit the increase of inflammatory cytokines in serum and liver and enhance the antioxidant capacity of weaned lambs, thus protecting lambs from the negative effects of weaning stress to a certain extent, and 2 g/kg of coated sodium butyrate is more reasonable.

Key words： sodium butyrate; weaned lamb; immune organ index; immune index; antioxidant index

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