动物营养学报 >

2021 , Vol. 33 >Issue 7: 4049 - 4056

DOI: https://doi.org/10.3969/j.issn.1006-267x.2021.07.046

分子与细胞营养 MOLECULAR AND CELLULAR NUTRITION

急性冷刺激对断奶仔猪十二指肠组织结构及核转录因子-κB/肠道碱性磷酸酶信号通路的影响

  • 乔新月 ,
  • 高炳南 ,
  • 任雨龙 ,
  • 李金库 ,
  • 陈妍 ,
  • 崔一喆 ,
  • 王秋菊
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  • 黑龙江八一农垦大学动物科技学院, 大庆 163319
乔新月(1995-),女,黑龙江绥化人,硕士研究生,从事动物营养与饲料科学研究。E-mail:2362943901@qq.com

收稿日期: 2021-01-07

  网络出版日期: 2021-07-06

基金资助

黑龙江省自然科学基金项目（C2014444）；黑龙江八一农垦大学研究生创新科研项目（YJSCX2019-Y27）；中国博士后科学基金会"第11批中国博士后科学基金特别资助"（2018T110320）；中国博士后科学基金面上一等资助（2017M6201024）；黑龙江八一农垦大学博士后校内启动项目（601038）

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Effects of Acute Cold Stimulation on Duodenal Histology Structure and Nuclear Transcription Factor-κB/Intestinal Alkaline Phosphatase Signaling Pathway of Weaned Piglets

  • QIAO Xinyue ,
  • GAO Bingnan ,
  • REN Yulong ,
  • LI Jinku ,
  • CHEN Yan ,
  • CUI Yizhe ,
  • WANG Qiuju
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  • College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing 163319, China

Received date: 2021-01-07

  Online published: 2021-07-06

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摘要

本试验旨在探究急性冷刺激对断奶仔猪十二指肠肠道结构及核转录因子-κB（NF-κB）/肠道碱性磷酸酶（IAP）信号通路的影响。试验选取16头30日龄的健康"杜×长×大"三元杂交断奶仔猪，随机分为4组，每组4个重复，每个重复1头仔猪。对照组饲养于温度为（24±2） ℃的环境中，3个急性冷刺激组在温度为（4±2） ℃的人工气候室中分别饲养2、6和12 h。急性冷刺激试验结束后屠宰各组仔猪，取十二指肠进行固定、石蜡切片、苏木精-伊红（HE）染色，观察肠黏膜形态变化，并检测肠绒毛长度、隐窝深度，同时应用Western-blot法检测十二指肠NF-κB/IAP信号通路关键蛋白的表达情况。结果表明：对照组仔猪十二指肠结构完整，绒毛排列紧密，急性冷刺激组仔猪十二指肠绒毛出现断裂和少数上皮细胞变性脱落现象，绒毛排列稀疏。急性冷刺激12 h时，仔猪十二指肠绒毛高度与对照组和急性冷刺激2、6 h时相比显著降低（P<0.05）。与对照组相比，3个急性冷刺激组隐窝深度及绒毛高度/隐窝深度均下降，但差异不显著（P>0.05）。在十二指肠中，与对照组相比，急性冷刺激2 h时，p65相对表达量略有升高，但差异不显著（P>0.05）；急性冷刺激6 h时，p65相对表达量基本恢复到冷刺激前（对照组）水平；与对照组和急性冷刺激6 h时相比，急性冷刺激12 h时p65相对表达量显著升高（P<0.05）；与对照组相比，急性冷刺激2和6 h时核转录因子抑制蛋白（IκB）相对表达量有增加的趋势，但差异不显著（P>0.05）；急性冷刺激12 h时IκB相对表达量与对照组相比极显著增加（P<0.01），与急性冷刺激2和6 h时相比也极显著增加（P<0.01）；与对照组相比，急性冷刺激2和6 h时磷酸化IκB（p-IκB）相对表达量略有增加，但差异不显著（P>0.05）；急性冷刺激12 h时p-IκB相对表达量与对照组相比极显著增加（P<0.01）；与对照组相比，急性冷刺激2、6和12 h时IAP相对表达量极显著升高（P<0.01）；与急性冷刺激2 h时相比，急性冷刺激6和12 h时IAP相对表达量极显著降低（P<0.01）。结果提示，急性冷刺激损伤断奶仔猪十二指肠黏膜结构，这可能与急性冷刺激激活NF-κB/IAP信号通路，并上调NF-κB/IAP通路相关蛋白的表达有关。

关键词： 急性冷刺激; 断奶仔猪; 十二指肠; NF-κ; B信号通路; IAP信号通路

本文引用格式

乔新月 , 高炳南 , 任雨龙 , 李金库 , 陈妍 , 崔一喆 , 王秋菊 . 急性冷刺激对断奶仔猪十二指肠组织结构及核转录因子-κB/肠道碱性磷酸酶信号通路的影响[J]. 动物营养学报, 2021 , 33(7) : 4049 -4056 . DOI: 10.3969/j.issn.1006-267x.2021.07.046

Abstract

The purpose of this study was to investigate the effects of acute cold stimulation on the duodenal histology structure and nuclear transcription factor-κB (NF-κB)/intestinal alkaline phosphatase (IAP) signaling pathway of weaned piglets. Sixteen healthy "Duroc×Landrace×Yorkshire" ternary hybrid weaned piglets at 30 days of age were selected and randomly divided into 4 groups with 4 replicates in each group and 1 piglet in each replicate. The control group was kept at (24±2)℃, while 3 acute cold stimulation groups were kept in an artificial climate chamber at (4±2)℃ at 2, 6 and 12 h, respectively. Piglets were slaughtered after the acute cold stimulation and duodenum was fixed, paraffin sectioned and hematoxylin-eosin (HE) stained. The morphology change of intestinal mucosa was observed in the sections, and the villi height and crypt depth were detected. Meanwhile, the expression of key proteins in the NF-κB/IAP signaling pathway in duodenum was detected by Western-blot method. The results showed that the duodenum of pigs in the control group had complete structure and close villi. In the acute cold stimulation groups, the villi were broken and a few epithelial cells denatured and shed. At 12 h under acute cold stimulation, the duodenal villi height of piglets was significantly lower than that of the control group and at 2 and 6 h under acute cold stimulation (P<0.05). Compared with the control group, the crypt depth and villi height/crypt depth in the 3 acute cold stimulation groups were decreased, but the differences were not significant (P>0.05). In the duodenum, the relative expression level of p65 was slightly increased compared with the control group at 2 h under acute cold stimulation, but the difference was not significant (P>0.05). At 6 h under acute cold stimulation, the relative expression level of p65 basically recovered to the level before cold stimulation (control group). At 12 h under acute cold stimulation, compared with the control group and at 6 h under acute cold stimulation, the relative expression level of p65 was significantly increased (P<0.05). Compared with the control group, the relative expression level of inhibitor of nuclear transcription factor-κB (IκB) at 2 and 6 h under acute cold stimulation showed an increased trend, with no significant difference (P>0.05). After 12 h under acute cold stimulation, the relative expression level of IκB increased extremely significantly compared with the control group (P<0.01), and compared with at 2 and 6 h under acute cold stimulation, it also increased significantly (P<0.01). Compared with the control group, the relative expression level of phosphorylated IκB (p-IκB) at 2 and 6 h under acute cold stimulation was slightly increased, with no significant difference (P>0.05). Compared with the control group, the relative expression level of p-IκB was extremely significantly increased at 12 h under acute cold stimulation (P<0.01). Compared with the control group, the relative expression level of IAP was extremely significantly increased at 2, 6 and 12 h under acute cold stimulation (P<0.01), and the relative expression level of IAP was extremely significantly decreased at 6 and 12 h under acute cold stimulation compared with 2 h under acute cold stimulation (P<0.01). The results indicated that acute cold stimulation can affect the intestinal mucosal structure of the duodenum, which may be related to the activation of NF-κB/IAP signaling pathway and the up-regulation of NF-κB/IAP signaling pathway related proteins by acute cold stimulation.

Key words： acute cold stimulation; weaned piglets; duodenum; NF-κ; B signaling pathway; IAP signaling pathway

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