分子与细胞营养 MOLECULAR AND CELLULAR NUTRITION

晋南牛固醇结合原件蛋白1基因在不同组织中表达规律及其与肌内脂肪含量的相关性研究

  • 程景 ,
  • 张元庆 ,
  • 王曦 ,
  • 张丹丹 ,
  • 李博 ,
  • 靳光 ,
  • 王栋才 ,
  • 徐芳 ,
  • 孙锐锋 ,
  • 梁圆 ,
  • 薛丽娜
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  • 山西农业大学(山西省农业科学院)动物科学学院, 太原 030032
程景(1988-),女,山西太谷人,助理研究员,硕士,研究方向为反刍动物营养与饲料科学。E-mail:chengjing1104@126.com

收稿日期: 2020-12-22

  网络出版日期: 2021-07-06

基金资助

山西省青年科技研究基金(201801D221309);农牧交错带牛羊牧繁农育关键技术集成示范项目(16200158);山西省现代农业产业技术体系(2017-05);山西省农业科学院农业科技创新研究课题(YCX2018D2YS03)

Expression Pattern of Sterol Regulatory Element Binding Protein 1 Gene in Different Tissues of Jinnan Cattle and Its Correlation Analysis with Intramuscular Fat Content

  • CHENG Jing ,
  • ZHANG Yuanqing ,
  • WANG Xi ,
  • ZHANG Dandan ,
  • LI Bo ,
  • JIN Guang ,
  • WANG Dongcai ,
  • XU Fang ,
  • SUN Ruifeng ,
  • LIANG Yuan ,
  • XUE Lina
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  • College of Animal Science, Shanxi Agricultural University (Shanxi Academy of Agricultural Science), Taiyuan 030032, China

Received date: 2020-12-22

  Online published: 2021-07-06

Supported by

 

摘要

为了研究固醇结合原件蛋白1(SREBP1)基因的表达及其参与脂肪酸代谢的调控机制,以及其与肌内脂肪(IMF)含量的关系,本试验选用相同饲养条件下晋南牛12头,屠宰后测定屠宰性状、背最长肌IMF含量、脂肪酸和氨基酸组分及心脏、脾脏、肾脏、肝脏、背最长肌(LP)、皮下脂肪(SF)、腹腔脂肪(AF)、胸腔脂肪(CF)中SREBP1基因相对表达量。根据IMF含量分为高肌内脂肪组(HIF组)和低肌内脂肪组(LIF组),比较2组LP、SF、AF、CF中SREBP1基因相对表达量的差异,并分析其与IMF含量的相关性。结果表明:LIF组胴体重、屠宰率均显著高于HIF组(P<0.05);HIF组IMF含量为12.30%,显著高于LIF组(P<0.05);LIF组的LP剪切力显著高于HIF组(P<0.05)。HIF组的LP中总脂肪酸(TFA)含量为9.698%,显著高于LIF组(P<0.05)。SREBP1基因在所测组织中均有表达,其中在LP中的相对表达量最高(P<0.05),在肾脏、肝脏和脂肪组织中次之;HIF组SREBP1基因在LP中的相对表达量极显著高于LIF组(P<0.01),在AF和CF中的相对表达量极显著低于LIF组(P<0.01);LP中SREBP1基因相对表达量与IMF含量呈极显著正相关(R2=0.987,P<0.01),AF、CF中SREBP1基因相对表达量与IMF含量则呈极显著负相关(AF:R2=-0.713、P<0.01,CF:R2=-0.946、P<0.01)。综上所述,晋南牛SREBP1基因在不同组织中表达量不同,以LP中相对表达量最高;不同IMF含量晋南牛SREBP1基因表达模式不同,且SREBP1基因正向调控LP中IMF沉积,可作为影响晋南牛IMF含量的候选基因。

本文引用格式

程景 , 张元庆 , 王曦 , 张丹丹 , 李博 , 靳光 , 王栋才 , 徐芳 , 孙锐锋 , 梁圆 , 薛丽娜 . 晋南牛固醇结合原件蛋白1基因在不同组织中表达规律及其与肌内脂肪含量的相关性研究[J]. 动物营养学报, 2021 , 33(7) : 4079 -4087 . DOI: 10.3969/j.issn.1006-267x.2021.07.049

Abstract

To explore the expression mechanism of sterol regulatory element binding protein 1 (SREBP1) gene of Jinnan cattle and its role in regulating fatty acid metabolism and the correlation with intramuscular fat (IMF), twelve Jinnan cattle were selected and raised under the same feeding and management conditions. Their slaughtering characteristics, as well as IMF content and fatty acid contents and amino acid composition of longissimus dorsi (LP) were measured after slaughter. The cattle were divided into high IMF content group (HIF group) and low IMF content group (LIF group). The SREBP1 gene expression levels in heart, spleen, kidney, liver, LP, subcutaneous fat (SF), chest fat (CF) and abdominal fat (AF) were detected by real time qPCR, and then their correlations with IMF content were analyzed. The shear force, carcass weight and slaughter rate of LIF group were significantly higher than those of HIF group (P<0.05). The IMF content of HIF group (12.30%) was significantly higher than that of LIF group (4.40%)(P<0.05). The total fatty acid (TFA) content in LP of HIF group was 9.698%, while that of LIF group was 4.064% (P<0.05). Compared with other tissues, the highest relative expression level of SREBP1 gene was in LP(P<0.05), which was relatively higher than that in kidney, liver and adipose tissue. The SREBP1 gene relative expression level in LP of HIF group was strongly significantly higher than that of LIF group (P<0.01). On the contrary, the SREBP1 gene relative expression level in AF and CF of HIF group was strongly significantly lower than that of LIF group (P<0.01). The relative expression level of SREBP1 gene in LP and SF were positively correlated with IMF content (R2=0.987, P<0.01);oppositely, it in the AF and CF were significantly negatively correlated with IMF content(AF:R2=-0.713,P<0.01;CF:R2=-0.946,P<0.01). In conclusion, the expression level of SREBP1 gene in Jinnan cattle is distinct in different tissues, of which the highest expression level is in LP. Also, SREBP1 positively regulates IMF deposition in LP. The expression pattern of SREBP1 gene in Jannan cattle with different IMF contents is different, and SREBP1 gene positively regulats the deposition of IMF in LP, so it can be used as a candidate gene to affect the IMF content in Jannan cattle.

参考文献

[1] LEE B,CHOI Y M.Correlation of marbling characteristics with meat quality and histochemical characteristics in Longissimus thoracis muscle from Hanwoo steers[J].Food Science of Animal Resources,2019,39(1):151-161.  
[2] PIAO M Y,YONG H I,LEE H J,et al.Comparison of fatty acid profiles and volatile compounds among quality grades and their association with carcass characteristics in longissimus dorsi and semimembranosus muscles of Korean cattle steer[J].Livestock Science,2017,198:147-156.
[3] CASTAGNINO P S,FIORENTINI G,DALLANTONIA E E,et al.Fatty acid profile and carcass traits of feedlot Nellore cattle fed crude glycerin and virginiamycin[J].Meat Science,2018,140:51-58..
[4] RAYCHAUDHURI S,YOUNG B P,ESPENSHADE P J,et al.Regulation of lipid metabolism:a tale of two yeasts[J].Current Opinion in Cell Biology,2012,24(4):502-508.  
[5] HORTON J D,GOLDSTEIN J L,BROWN M S.SREBPs:activators of the complete program of cholesterol and fatty acid synthesis in the liver[J].The Journal of Clinical Investigation,2002,109(9):1125-1131.  
[6] CARCANGIU V,MURA M C,DAGA C,et al.Association between SREBP-1 gene expression in mammary gland and milk fat yield in Sarda breed sheep[J].Meta Gene,2013,1:43-49.
[7] HUANG W C,LI X Y,LIU J,et al.Activation of androgen receptor,lipogenesis,and oxidative stress converged by SREBP-1 is responsible for regulating growth and progression of prostate cancer cells[J].Molecular Cancer Research,2012,10(1):133-142.  
[8] 董金玲,谢志宏,何杰,等.慢性丙型肝炎合并脂肪肝患者Sirt1和固醇调节元件结合蛋白表达及意义[J].中华实验和临床感染病杂志(电子版),2018,12(6):577-584. DONG J L,XIE Z H,HE J,et al.Expression and clinical significance of Sirt1 and sterol-regulatory element binding proteins in hepatitis C virus infected patients with fatty liver[J].Chinese Journal of Experimental and Clinical Infectious Diseases (Electronic Edition),2018,12(6):577-584.(in Chinese)
[9] MVLLER-WIELAND D,KNEBEL B,HAAS J,et al.SREBP-1 and fatty liver.Clinical relevance for diabetes,obesity,dyslipidemia and atherosclerosis[J].Herz,2012,37(3):273-280.  
[10] SCHMITTGEN T D,LIVAK K J.Analyzing real-time PCR data by the comparative CT method[J].Nature Protocols,2008,3(6):1101-1108.  
[11] 王小梅,敖日格乐,王纯洁.杂交肉牛背最长肌LPL基因与脂肪代谢的研究[J].黑龙江畜牧兽医,2018(21):100-102. WANG X M,AORI G L,WANG C J.Studies on LPL gene and fat metabolism of longissimus dorsi muscle in hybrid beef cattle[J].Heilongjiang Animal Science and Veterinary Medicine,2018(21):100-102.(in Chinese)
[12] 张海波.紫苏籽提取物对育肥牛肌内脂肪沉积的影响[J].动物营养学报,2019,31(4):1897-1903. ZHANG H B.Effects of perilla seed extract on intramuscular fat deposition in fattening cattle[J].Chinese Journal of Animal Nutrition,2019,31(4):1897-1903.(in Chinese)
[13] UEDA Y,WATANABE A,HIGUCHI M,et al.Effects of intramuscular fat deposition on the beef traits of Japanese Black steers (Wagyu)[J].Animal Science Journal,2010,78(2):189-194.
[14] 唐慧,王巍,方东辉,等.不同品种牛SCD1基因表达与肌肉脂肪酸组成的关系研究[J].黑龙江畜牧兽医,2018(1):1-4,9. TANG H,WANG W,FANG D H,et al.Study on the relationship between SCD1 gene expression level and muscle fatty acid composition in different breeds of cattle[J].Heilongjiang Animal Science and Veterinary Medicine,2018(1):1-4,9. (in Chinese)
[15] 吴磊,刘瑞莉,袁玮,等.CRTC2基因在不同品种和月龄肉牛的表达分析[J].畜牧与兽医,2019,51(10):9-14. WU L,LIU R L,YUAN W,et al.Analysis of CRTC2 gene expression in cattle of different varieties and months of age[J].Animal Husbandry & Veterinary Medicine,2019,51(10):9-14. (in Chinese)
[16] 毛衍伟,张一敏,朱立贤,等.肌内脂肪对牛背最长肌品质的影响及肌内脂肪沉积机制的研究[J].食品与发酵工业,2018,44(11):89-96. MAO Y W,ZHANG Y M,ZHU L X,et al.Effect of intramuscular fat on beef longissimus dorsi quality and its deposition mechanisms[J].Food and Fermentation Industries,2018,44(11):89-96. (in Chinese)
[17] 杨勤,官久强,柴志欣,等.低海拔舍饲对牦牛肌肉品质的影响研究[J].草业学报,2020,29(5):33-42. YANG Q,GUAN Y Q,CHAI Z X,et al.Effect of indoor-feeding at low altitude on muscle quality of yaks[J].Acta Prataculturae Sinica,2020,29(5):33-42. (in Chinese)
[18] PARK S J,BEAK S H,JUNG D J S,et al.Genetic,management,and nutritional factors affecting intramuscular fat deposition in beef cattle-A review[J].Asian-Australasian Journal of Animal Sciences,2018,31(7):1043-1061.  
[19] LIANG R R,ZHU H,MAO Y W,et al.Tenderness and sensory attributes of the longissimus lumborum muscles with different quality grades from Chinese fattened yellow crossbred steers[J].Meat Science,2016,112:52-57.
[20] 农秋雲,刘嘉琪,单体中.猪肉中脂肪酸组成的品种差异及脂肪酸沉积的调控机制[J].动物营养学报,2019,31(6):2507-2514. NONG Q Y,LIU J Q,SHAN T Z.Breed difference of fatty acid composition and regulation mechanism of fatty acid deposition in porkchinese[J].Chinese Journal of Animal Nutrition,2019,31(6):2507-2514. (in Chinese)
[21] ZHANG H B,GUAN W K.The response of gene expression associated with intramuscular fat deposition in the longissimus dorsi muscle of Simmental×yellow breed cattle to different energy levels of diets[J].Animal Science Journal,2019,90(4):493-503.  
[22] WATANABE A,UEDA Y,HIGUCHI M.Effects of slaughter age on the levels of free amino acids and dipeptides in fattening cattle[J].Animal Science Journal,2004,75(4):361-367.  
[23] 张崇本.脂肪细胞的分化及调控[J].生理科学进展,2004,35(1):7-12. ZHANG C B.Adipocyte differentiation and its regulation[J].Progress in Physiological Sciences,2004,35(1):7-12. (in Chinese)
[24] 黄胥莱,陈东,唐江权,等.SREBP-1基因在湘西黄牛不同组织中的发育性表达研究[J].中国草食动物科学,2020,40(3):8-11. HUANG X L,CHEN D,DONG T J,et al.Study on the developmental expression of SREBP-1 gene in different tissues of Xiangxi cattle[J].China Herbivore Science,2020,40(3):8-11. (in Chinese)
[25] ZHANG D W,TOMISATO W,SU L J,et al.Skin-specific regulation of SREBP processing and lipid biosynthesis by glycerol kinase 5[J].Proceedings of the National Academy of Sciences of the United States of America,2017,114(26):E5197-E5206.
[26] GRIFFITHS B,LEWIS C A,BENSAAD K,et al.Sterol regulatory element binding protein-dependent regulation of lipid synthesis supports cell survival and tumor growth[J].Cancer Metab,2013,1:3.
[27] 官久强,张海波,罗晓林.舍饲牦牛与犏牛生长性能、屠宰性能、肉品质和肌内脂肪沉积相关基因表达的比较分析[J].动物营养学报,2019,31(6):2659-2665. GUAN J Q,ZHANG H B,LUO X L.Comparative study on growth performance,slaughter performance,meat quality and intramuscular fat deposition related gene expression between dzo and yak in house feeding[J].Chinese Journal of Animal Nutrition,2019,31(6):2659-2665. (in Chinese)
[28] 鞠大鹏,詹丽杏.脂肪细胞分化及其调控的研究进展[J].中国细胞生物学学报,2010,32(5):690-695. JU D P,ZHAN L X.Decelopments in regulation of adipocytes differentiation[J].Chinese Journal of Cell Biology,2010,32(5):690-695. (in Chinese)
[29] 金世杰,李香子,高青山,等.延边黄牛SREBP1和SCD1基因表达的发育性变化及与肌间脂肪含量的关联分析[J].饲料工业,2015,36(3):37-42. JIN S J,LI X Z,GAO Q S,et al.Developmental changes of gene expression of SREBP1 and SCD1 genes and their association analysis IMF in Yanbian cattle[J].Feed Industry,2015,36(3):37-42. (in Chinese)
[30] 徐丽.牛SREBP1基因与牛肉肌内脂肪沉积的研究[D].硕士学位论文.北京:中国农业科学院,2013:59. XU L.Study on beef intramuscular fat deposition of SREBP1 gene in cattle[D].Master's Thesis.Beijing:Chinese Academy of Agricultural Sciences.2013:59. (in Chinese)
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