动物营养学报 >

2021 , Vol. 33 >Issue 7: 4183 - 4193

DOI: https://doi.org/10.3969/j.issn.1006-267x.2021.07.059

研究简报 SHORT COMMUNICATION

鸡肉粉替代秘鲁鱼粉对大口黑鲈生长性能、血清生化指标、肠道及肝脏组织结构的影响

  • 马利 ,
  • 穆苑 ,
  • 李维 ,
  • 耿旭 ,
  • 蒋明 ,
  • 文华 ,
  • 王国霞 ,
  • 李勇
展开
  • 1. 中粮饲料有限公司, 北京 100020;
    2. 中国水产科学研究院长江水产研究所, 武汉 430223;
    3. 广东省农业科学院动物科学研究所, 农业部华南动物营养与饲料重点实验室, 广东省畜禽育种与营养研究重点实验室, 广州 510640
马利(1977-),男,河南鹤壁人,助理研究员,硕士,从事水产动物营养与饲料研究。E-mail:hnmlhb@163.com

收稿日期: 2020-12-20

  网络出版日期: 2021-07-06

收起

Effects of Peru Fish Meal Replacement by Pet Grade Chicken Meal on Growth Performance, Serum Biochemical Indexes, Intestinal and Liver Histomorphology of Largemouth Bass (Micropterus salmoides)

  • MA Li ,
  • MU Yuan ,
  • LI Wei ,
  • GENG Xu ,
  • JIANG Ming ,
  • WEN Hua ,
  • WANG Guoxia ,
  • LI Yong
Expand
  • 1. COFCO Feed Co., Ltd., Beijing 100020, China;
    2. Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China;
    3. Guangdong Key Laboratory of Animal Breeding and Nutrition, Key Laboratory of Animal Nutrition and Feed Science in South China of Ministry of Agriculture, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China

Received date: 2020-12-20

  Online published: 2021-07-06

Fold

摘要

本试验旨在研究实用饲料配方中宠物级鸡肉粉替代秘鲁鱼粉对大口黑鲈生长性能、血清生化指标、肠道和肝脏组织结构的影响。选用初始体质量约为6.88 g的大口黑鲈700尾，随机分为5组，每组4个重复，每个重复35尾。用宠物级鸡肉粉替代秘鲁鱼粉，分别配制5种试验饲料（标记为T1、T2、T3、T4和T5），其中T1含有秘鲁鱼粉30%和宠物级鸡肉粉10%，T2~T5中的鸡肉粉以7.5%（占比饲料）的梯度增加，养殖试验为期56 d。结果表明：各试验组间的生长性能、饲料系数和形态学指标无显著差异（P>0.05）。各试验组间的全鱼体成分、蛋白质和脂肪沉积率无显著差异（P>0.05），T4组粗灰分沉积率显著高于T1和T2组（P<0.05）。鸡肉粉替代秘鲁鱼粉对大口黑鲈的血清生化指标、酸性磷酸酶、碱性磷酸酶活性和总抗氧化能力均无显著影响（P>0.05）；鸡肉粉替代秘鲁鱼粉对大口黑鲈肠道脂肪酶、胰蛋白酶活性均无显著影响（P>0.05），对肠道淀粉酶活性有显著影响（P<0.05）。各试验组间的肠道绒毛高度、肌层厚度、杯状细胞数均无显著差异（P>0.05），T5组肠道绒毛宽度显著高于T2和T3组（P<0.05）；各试验组肝脏组织未见坏死，T1~T4组的肝细胞正常，T5组肝细胞轮廓界限不清，细胞核偏向细胞一侧较多。以上结果表明，在本试验条件下，饲料中添加32.5%或以下的鸡肉粉对大口黑鲈生长性能、肠道及肝脏结构没有不利影响，40.0%的鸡肉粉对大口黑鲈的肝脏和肠道有负面影响。

关键词： 大口黑鲈; 鸡肉粉; 鱼粉替代; 生长性能; 血清生化指标; 肠道及肝脏组织结构

本文引用格式

马利 , 穆苑 , 李维 , 耿旭 , 蒋明 , 文华 , 王国霞 , 李勇 . 鸡肉粉替代秘鲁鱼粉对大口黑鲈生长性能、血清生化指标、肠道及肝脏组织结构的影响[J]. 动物营养学报, 2021 , 33(7) : 4183 -4193 . DOI: 10.3969/j.issn.1006-267x.2021.07.059

Abstract

This study was conducted to evaluate the effects of Peru fish meal replacement by pet grade chicken meal on the growth performance, serum biochemical indexes, intestinal and liver histomorphology of Micropterus salmoides. Five experimental diets (labeled as T1, T2, T3, T4 and T5) were prepared. T1 contained 30% Peru fish meal and 10% pet grade chicken meal, while the pet grade chicken meal in T2 to T5 increased by a gradient of 7.5% (proportion of the diet). The 700 largemouth bass with an initial average weight of 6.88 g were selected and randomly divided into 5 groups with 4 replicates in each group, and 35 fish in each replicate. The feeding trial lasted for 56 d. The results showed that there were no significant differences in growth performance, feed utilization and body morphological indexes (P>0.05). There was no significant difference in composition and nutrient deposition of whole fish in each test group (P>0.05). The replacement of pet grade chicken meal for Peru fish meal had no significant effect on activities of serum acid phosphatase and alkaline phosphatase, and total antioxidant capacity of largemouth bass (P>0.05). The replacement had no significant effect on the activities of intestinal lipase and trypsin of largemouth bass (P>0.05), but it had a significant effect on the activities of intestinal amylase (P<0.05). There were no significant changes in the height of intestinal villi, muscle thickness, and number of goblet cells in each experimental group (P>0.05), and the villi width in T5 group was significantly greater than that in T2 group and T3 group (P<0.05). No liver tissue necrosis was observed in each group. The hepatocyte morphology showed that the contour boundary of liver cells was not clear, and the nucleus was more tilted to the side of the cells in the T5 group. However, the hepatocytopathy were not severe in the T1 group to T4 group. The above results indicate that pet grade chicken meal can use 32.5% in the diet for largemouth bass based on the growth performance. However,the inclusion of 40.0% pet grade chicken meal in the diet has negative effects on the liver health of largemouth bass.

参考文献

[1] NRC.Nutrient requirements of fish and shrimp[S].Washington D.C.:The National Academies Press,2011.
[2] MA Y C,LI M M,XIE D Z,et al.Fishmeal can be replaced with a high proportion of terrestrial protein in the diet of the carnivorous marine teleost (Trachinotus ovatus)[J].Aquaculture,2020,519:734910.
[3] 麦康森.中国水产动物营养研究与饲料工业的发展历程与展望[J].饲料工业,2020,41(1):2-6. MAI K S.History and prospect of aquaculture nutrition and aquafeed industry of China[J].Feed Industry Magazine,2020,41(1):2-6. (in Chinese)
[4] 张新节,王小城,迟淑艳.鸡肉粉替代鱼粉对金鲳鱼生长、体成分及血清指标的影响[J].饲料博览,2019(11):1-6. ZHANG X J,WANG X C,CHI S Y.Effects of poultry by-product meal as the replacement of fish meal in the diets on the growth performance,body composition,serum biochemical indexes of ovatepompano (Trachinotus ovatus)[J].Feed Review,2019(11):1-6.(in Chinese)
[5] 曹晓莉,钟蕾,戴振炎,等.宠物级鸡肉粉替代鱼粉对黄鳝生长性能、肠道消化酶活性及血清生化指标的影响[J].动物营养学报,2020,32(5):2352-2360. CAO X L,ZHONG L,DAI Z Y,et al.Effects of fish meal replacement by pet grate poultry by-product meal on growth performance,intestinal digestive enzyme activities and serum biochemical indexes of rice filed eel (Monopterus albus)[J].Chinese Journal of Animal Nutrition,2020,32(5):2352-2360.(in Chinese)
[6] WANG Y,WANG F,JI W X,et al.Optimizing dietary protein sources for Japanese sea bass (Lateolabrax japonicas) with an emphasis on using poultry by-product meal to substitute fish meal[J].Aquaculture Research,2015,46(4):874-883.  
[7] ABDUL-HALIM H H,ALIYU-PAIKO M,HASHIM R.Partial replacement of fish meal with poultry by-product meal in diets for Snakehead,Channa striata (Bloch,1793),fingerlings[J].Journal of the World Aquaculture Society,2014,45(2):233-241.  
[8] SHAPAWI R,NG W K,MUSTAFA S.Replacement of fish meal with poultry by-product meal in diets formulated for the Humpback grouper,Cromileptes Altivelis[J].Aquaculture,2007,273(1):118-126.  
[9] 胡海滨,解绶启,钱雪桥,等.饲料中添加玉米蛋白粉或鸡肉粉替代部分鱼粉对卵形鲳鲹生长性能的影响[J].动物营养学报,2019,31(6):2752-2764. HU H B,XIE S Q,QIAN X Q,et al.Effects of dietary corn gluten meal or poultry meal partially replacing fish meal on growth performance of Golden pompano (Trachinotus ovatus)[J].Chinese Journal of Animal,2019,31(6):2752-2764. (in Chinese)
[10] MCLEAN E,BARROWS F T,CRAIG S R,et al.Complete replacement of fishmeal by soybean and poultry meals in Pacific whiteleg shrimp feeds:growth and tolerance to EMS/AHPND and WSSV challenge[J].Aquaculture,2020,527:735383.
[11] SIN N N N,MUSTAFA S,SUYON,et al.Efficient utilization of poultry by-product meal-based diets when fed to Giant freshwater prawn,Macrobrachium rosenbergii[J].Journal of Applied Aquaculture,2020:1-20,doi:10.1080/10454438.2019.1709599.
[12] 李宗升,吴立新,李思萌,等.鸡肉粉替代鱼粉对大菱鲆生长和体组成的影响[J].水产科学,2016,35(5):486-491. LI Z S,WU L X,LI S M,et al.Effects of replacing fish meal with poultry by-product meal on growth performance and body composition of Juvenile turbot (Scophthalmus maximus)[J].Fisheries Science,2016,35(5):486-491. (in Chinese)
[13] DING G T,LI S L,WANG A,et al.Effect of chicken haemoglobin powder on growth,feed utilization,immunity and haematological index of largemouth bass (Micropterus Salmoides)[J].Aquaculture and Fisheries,2020,5(4):187-192.  
[14] LI X Y,ZHENG S X,MA X K,et al.Effects of dietary starch and lipid levels on the protein retention and growth of largemouth bass (Micropterus salmoides)[J].Amino Acids,2020,52(6):999-1016.
[15] LI X Y,ZHENG S X,MA X K,et al.Effects of dietary protein and lipid levels on the growth performance,feed utilization,and liver histology of largemouth bass (Micropterus Salmoides)[J].Amino Acids,2020,52(6):1043-1061.
[16] MA H J,MOU M M,PU D C,et al.Effect of dietary starch level on growth,metabolism enzyme and oxidative status of Juvenile largemouth bass,Micropterus salmoides[J].Aquaculture,2019,498:482-487.
[17] 陈乃松,肖温温,梁勤朗,等.饲料中脂肪与蛋白质比对大口黑鲈生长、体组成和非特异性免疫的影响[J].水产学报,2012,36(8):1270-1280. CHEN N S,XIAO W W,LIANG Q L,et al.Effects of dietary lipid to protein ratios on growth performance,body composition and non-specific immunity of largemouth bass (Micropterus salmoides)[J].Journal of Fisheries of China,2012,36(8):1270-1280. (in Chinese)
[18] CAI Z N,QIAN X Q,XIE S Q.Optimal dietary protein concentrations for largemouth bass (Micropterus salmoides) of different sizes (10 to 500 g)[J].Aquaculture International,2020,28(2):831-840.  
[19] 朱旺明,谭永刚,马卉佳,等.不同水平南极磷虾粉等量替代鱼粉对大口黑鲈生长性能及部分生理生化指标的影响[J].中国饲料,2019(15):85-92. ZHOU W M,TAN Y G,MA H J,et al.Effects of different levels of krill meal replacement with fish meal on growth performance and some physiological and biochemical indexes of largemouth bass[J].China Feed,2019(15):85-92. (in Chinese)
[20] HE M,LI X Q,POOLSAWAT L,et al.Effects of fish meal replaced by fermented soybean meal on growth performance,intestinal histology and microbiota of largemouth bass (Micropterus salmoides)[J].Aquaculture Nutrition,2020,26(4):1058-1071.  
[21] 易新文,陈瑞爱,徐家华.鸡肉粉替代鱼粉对卵形鲳鲹生长、饲料利用和抗氧化力的影响[J].中国海洋大学学报(自然科学版),2019,49(12):17-24. YI X W,CHEN R A,XU J H.Effects of the poultry by-product meal replacing partially dietary fishmeal on the growth,feed utilization and anti-oxidative capacity of golden pompano (Trachinotus ovatus)[J].Periodical of Ocean University of China(Natural Science Edition),2019,49(12):17-24. (in Chinese)
[22] ZHU H,GONG G,WANG J,et al.Replacement of fish meal with blend of rendered animal protein in diets for Siberian sturgeon (Acipenser baerii Brandt),results in performance equal to fish meal fed fish[J].Aquaculture Nutrition,2011,17(2):e389-e395.
[23] 易魁,陈庭,孙云章,等.鸡肉粉替代鱼粉饲料添加明胶、淀粉包被的氨基酸对凡纳滨对虾生长性能的影响[J].养殖与饲料,2018(3):5-10. YI K,CHEN T,SUN Y,et al.Effects of replacement of fish meal by poultry meal on growth performance of pacific white shrimp (Litopenaeus vannamei)[J].Journal of Breeding and feed,2018(3):5-10.(in Chinese)
[24] JIANG M,WEN H,GOU G W,et al.Preliminary study to evaluate the effects of dietary bile acids on growth performance and lipid metabolism of juvenile genetically improved farmed tilapia (Oreochromis Niloticus) fed plant ingredient-based diets[J].Aquaculture Nutrition,2018,24(4):1175-1183.  
[25] 黄文庆,黄燕华,米海峰,等.3种动物蛋白质源替代鱼粉对草鱼生长性能、肌肉营养成分、消化酶活性、血清生化和抗氧化指标的影响[J].动物营养学报,2019,31(5):2187-2200. HUANG W Q,HUANG Y H,MI H F,et al.Effects of fishmeal replacement by three animal protein sources on growth performance,muscle nutritional components,digestive enzyme activities and serum biochemical and antioxidant indices of Ctenopharyngodon idellus[J].Chinese Journal of Animal Nutrition,2019,31(5):2187-2200.(in Chinese)
[26] 刘猛,叶元土,蔡春芳,等.湖泊野生团头鲂健康评价指标体系的研究[J].上海水产海洋大学学报,2013,22(2):178-188. LIU M,YE Y T,CAI C F,et al.The research of health evaluation index system of lake wild Megalobrama amblycephala[J].Journal of Shanghai Ocean University,2013,22(2):178-188. (in Chinese)
[27] 欧红霞.不同饲料对大口黑鲈消化酶及肠道形态结构的影响[D].硕士学位论文.上海:上海海洋大学,2018. OU H X.Effects of different feed on digestive enzyme and intestinal morphologic structure of Micropterus salmoides[D].Master's Thesis.Shanghai:Shanghai Ocean University,2018.(in Chinese)
[28] 钟国防,田鑫鑫,谢春元,等.芽孢杆菌有氧发酵豆粕替代鱼粉对大口黑鲈幼鱼生长性能、血清生化指标及肠组织结构的影响[J/OL].渔业科学进展,2020.(2020-08-13).https://kns.cnki.net/kcms/detail/37.1466.S.20200803.1006.001.html. ZHONG G F,TIAN X X,XIE C Y,et al.Effects of bacillus aerobic fermentation of soybean meal replacing fish meal on the growth performance,serum biochemical indices and intestinal histology of largemouth bass (Micropterus salmoides)[J/OL].Progress in Fishery Sciences,2020.(2020-08-13).https://kns.cnki.net/kcms/detail/37.1466.S.20200803.1006.001.html. (in Chinese)
Options
文章导航

/

〈 〉

    联系我们

  • 地址：北京市海淀区圆明园西路2号
    　　　中国农业大学动科动医大楼153室
  • 邮编：100193
  • 电话：010－62817823
  • 网址：http://www.chinajan.com
  • E-mail：cjan@chinajan.com
京公网安备 11010802041926号    京ICP备17050989号-2