综述 REVIEW

木聚糖酶对肉鸡生长性能和肠道健康的影响

  • 王剑 ,
  • 尚庆辉 ,
  • 张连华 ,
  • 朴香淑
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  • 中国农业大学动物科技学院, 动物营养学国家重点实验室, 北京 100193
王剑(1996-),女,辽宁朝阳人,博士研究生,从事动物营养与饲料科学研究。E-mail:S20173040498@cau.edu.cn

收稿日期: 2021-02-05

  网络出版日期: 2021-08-11

基金资助

国家自然科学基金项目(31772612);北京自然科学基金项目(6202019);现代农业产业技术体系建设专项基金(CARS-35);农业农村部畜牧兽医局"政府购买服务项目"(2020年)——生长育肥猪常用蛋白饲料原料营养价值评定与参数建立(16200143)

Effects of Xylanases on Growth Performance and Gut Health of Broilers

  • WANG Jian ,
  • SHANG Qinghui ,
  • ZHANG Lianhua ,
  • PIAO Xiangshu
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  • State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China

Received date: 2021-02-05

  Online published: 2021-08-11

Supported by

 

摘要

木聚糖酶已被广泛应用于单胃动物饲粮中,木聚糖酶高效水解木聚糖的作用效果越来越受到关注,尤其在肉鸡的小麦基础饲粮中,旨在改善动物肠道健康。本文结合国内外研究进展,就木聚糖的抗营养特性以及木聚糖酶对肉鸡的促生长和改善肠道健康等作用效果进行了综述。

本文引用格式

王剑 , 尚庆辉 , 张连华 , 朴香淑 . 木聚糖酶对肉鸡生长性能和肠道健康的影响[J]. 动物营养学报, 2021 , 33(8) : 4231 -4243 . DOI: 10.3969/j.issn.1006-267x.2021.08.004

Abstract

At the present, xylanases have been widely applied in monogastric animal diets to improve gut health, especially in wheat-based diets for broilers. The xylanase has attracted extensive attention because it could efficiently hydrolyze xylan. Therefore, combined with the research progress at home and abroad, this paper primarily reviews anti-nutritional effects of xylan, and positive effects of xylanase on growth performance and gut health of broilers.

Key words: xylanase; broiler; gut health

参考文献

[1] RAZA A,BASHIR S,TABASSUM R.An update on carbohydrases:growth performance and intestinal health of poultry[J].Heliyon,2019,5(4):e01437.
[2] 高俊勤.木聚糖酶对肉仔鸡肠道消化特性及微生物区系的影响[D].硕士学位论文.南京:南京农业大学,2007. GAO J Q.The effects of xylanase on the nutrient digestibility and microflora of the gastrointestinal tract of broiler chickens[D].Master's Thesis.Nanjing:Nanjing Agricultural University,2007.(in Chinese)
[3] 尚庆辉,朴香淑,王玉磷.木聚糖酶在畜禽饲料中应用效果及其机理的研究进展[J].饲料工业,2017,38(2):17-24. SHANG Q H,PIAO X S,WANG Y L.Application effects and mechanism of xylanase in animal feed[J].Feed Industry,2017,38(2):17-24.(in Chinese)
[4] TELLEZ G,LATORRE J D,KUTTAPPAN V A,et al.Utilization of rye as energy source affects bacterial translocation,intestinal viscosity,microbiota composition,and bone mineralization in broiler chickens[J].Frontiers in Genetics,2014,5:339.
[5] XING H G,ZOU G,LIU C Y,et al.Improving the thermostability of a GH11 xylanase by directed evolution and rational design guided by B-factor analysis[J].Enzyme and Microbial Technology,2021,143:109720.
[6] ABD EL-WAHAB A,LINGENS J B,CHUPPAVA B,et al.Impact of rye inclusion in diets for broilers on performance,litter quality,foot pad health,digesta viscosity,organ traits and intestinal morphology[J].Sustainability,2020,12(18):7753.
[7] QESHMI F I,HOMAEI A,FERNANDES P,et al.Xylanases from marine microorganisms:a brief overview on scope,sources,features and potential applications[J].Biochimica et Biophysica Acta:Proteins and Proteomics,2020,1862(2):140312.
[8] UDAY U S P,CHOUDHURY P,BANDYOPADHYAY T K,et al.Classification,mode of action and production strategy of xylanase and its application for biofuel production from water hyacinth[J].International Journal of Biological Macromolecules,2016,82:1041-1054.
[9] JUTURU V,WU J C.Microbial xylanases:engineering,production and industrial applications[J].Biotechnology Advances,2012,30(6):1219-1227.  
[10] 杨雯涵.硫色曲霉酸性木聚糖酶耐热性改造研究[D].硕士学位论文.北京:中国农业大学,2015. YANG W H.Study on heat resistant modification of Aspergillus sulphureus acidic xylanase[D].Master's Thesis.Beijing:China Agricultural University,2015.(in Chinese)
[11] BHARDWAJ N,KUMAR B,VERMA P.A detailed overview of xylanases:an emerging biomolecule for current and future prospective[J].Bioresources and Bioprocessing,2019,6:40.
[12] SALEEM A,WARIS S,AHMED T,et al.Biochemical characterization and molecular docking of cloned xylanase gene from Bacillus subtilis RTS expressed in E. coli[J].International Journal of Biological Macromolecules,2021,168:310-321.
[13] LAI Z H,ZHOU C,MA X C,et al.Enzymatic characterization of a novel thermostable and alkaline tolerant GH10 xylanase and activity improvement by multiple rational mutagenesis strategies[J].International Journal of Biological Macromolecules,2020,170:164-177.
[14] WANG K,CAO R T,WANG M L,et al.A novel thermostable GH10 xylanase with activities on a wide variety of cellulosic substrates from a xylanolytic Bacillus strain exhibiting significant synergy with commercial celluclast 1.5 L in pretreated corn stover hydrolysis[J].Biotechnology for Biofuels,2019,12:48.
[15] KUMAR V,SATYANARAYANA T.Production of endoxylanase with enhanced thermostability by a novel polyextremophilic Bacillus halodurans TSEV1 and its applicability in waste paper deinking[J].Process Biochemistry,2014,49(3):386-394.  
[16] SUKHUMSIRICHART M,DEESUKON W,KAWAKAMI T,et al.Expression and characterization of recombinant GH11 xylanase from thermotolerant Streptomyces sp. SWU10[J].Applied Biochemistry and Biotechnology,2014,172(1):436-446.  
[17] JIANG X B,LIN J C,LIANG S Z,et al.High-efficient expression and pilot scale fermentation of Streptomyces xylanase from a constitutive Pichia pastoris vector[J].Food Biotechnology,2013,27(1):54-65.  
[18] MHIRI S,BOUANANE-DARENFED A,JEMLI S,et al.A thermophilic and thermostable xylanase from Caldicoprobacter algeriensis:recombinant expression,characterization and application in paper biobleaching[J].International Journal of Biological Macromolecules,2020,164:808-817.
[19] YU J,LIU X Q,GUAN L Y,et al.High-level expression and enzymatic properties of a novel thermostable xylanase with high arabinoxylan degradation ability from Chaetomium sp. suitable for beer mashing[J].International Journal of Biological Macromolecules,2021,168:223-232.
[20] ZHAO L,MENG K,BAI Y G,et al.Two family 11 xylanases from Achaetomium sp. Xz-8 with high catalytic efficiency and application potentials in the brewing industry[J].Journal of Agricultural and Food Chemistry,2013,61(28):6880-6889.  
[21] OUEPHANIT C,BOONVITTHYA N,BOZONNET S,et al.High-level heterologous expression of endo-1,4-β-xylanase from Penicillium citrinum in Pichia pastoris X-33 directed through codon optimization and optimized expression[J].Molecules,2019,24(19):3515.
[22] GIL-DURÁN C,RAVANAL M C,UBILLA P,et al.Heterologous expression,purification and characterization of a highly thermolabile endoxylanase from the Antarctic fungus Cladosporium sp.[J].Fungal Biology,2018,122(9):875-882.  
[23] HUANG Y H,ZHENG X L,PILGAARD B,et al.Identification and characterization of GH11 xylanase and GH43 xylosidase from the chytridiomycetous fungus,Rhizophlyctis rosea[J].Applied Microbiology and Biotechnology,2019,103(2):777-791.  
[24] WANG X Y,MA R,XIE X M,et al.Thermostability improvement of a Talaromyces leycettanus xylanase by rational protein engineering[J].Scientific Reports,2017,7:15287.
[25] BHARDWAJ N,KUMAR N,AGARWAL K,et al.Purification and characterization of a thermo-acid/alkali stable xylanases from Aspergillus oryzae LC1 and its application in xylo-oligosaccharides production from lignocellulosic agricultural wastes[J].International Journal of Biological Macromolecules,2019,122:1191-1202.
[26] CHADHA B S,KAUR B,BASOTRA N,et al.Thermostable xylanases from thermophilic fungi and bacteria:current perspective[J].Bioresource Technology,2019,277:195-203.
[27] CHAKDAR H,KUMAR M,PANDIYAN K,et al.Bacterial xylanases:biology to biotechnology[J].3 Biotech,2016,6(2):150.
[28] LUO D Y,YANG F X,YANG X J,et al.Effects of xylanase on performance,blood parameters,intestinal morphology,microflora and digestive enzyme activities of broilers fed wheat-based diets[J].Asian-Australasian Journal of Animal Sciences,2009,22(9):1288-1295.  
[29] HEDEMANN M S,THEIL P K,KNUDSEN K E B.The thickness of the intestinal mucous layer in the colon of rats fed various sources of non-digestible carbohydrates is positively correlated with the pool of SCFA but negatively correlated with the proportion of butyric acid in digesta[J].British Journal of Nutrition,2009,102(1):117-125.  
[30] RAVN J L,MARTENS H J,PETTERSSON D,et al.A commercial GH 11 xylanase mediates xylan solubilisation and degradation in wheat,rye and barley as demonstrated by microscopy techniques and wet chemistry methods[J].Animal Feed Science and Technology,2016,219:216-225.
[31] CHOCT M,HUGHES R J,BEDFORD M R.Effects of a xylanase on individual bird variation,starch digestion throughout the intestine,and ileal and caecal volatile fatty acid production in chickens fed wheat[J].British Poultry Science,1999,40(3):419-422.  
[32] KIARIE E,ROMERO L F,RAVINDRAN V.Growth performance,nutrient utilization,and digesta characteristics in broiler chickens fed corn or wheat diets without or with supplemental xylanase[J].Poultry Science,2014,93(5):1186-1196.  
[33] KHADEM A,LOURENCO M,DELEZIE E,et al.Does release of encapsulated nutrients have an important role in the efficacy of xylanase in broilers?[J].Poultry Science,2016,95(5):1066-1076.  
[34] KARLSSON E N,SCHMITZ E,LINARES-PASTÉN J A,et al.Endo-xylanases as tools for production of substituted xylooligosaccharides with prebiotic properties[J].Applied Microbiology and Biotechnology,2018,102(21):9081-9088.  
[35] MORGAN N K,WALLACE A,BEDFORD M R,et al.In vitro versus in situ evaluation of xylan hydrolysis into xylo-oligosaccharides in broiler chicken gastrointestinal tract[J].Carbohydrate Polymers,2020,230:115645.
[36] DALE T,HANNAY I,BEDFORD M R,et al.The effects of exogenous xylanase supplementation on the in vivo generation of xylooligosaccharides and monosaccharides in broilers fed a wheat-based diet[J].British Poultry Science,2020,61(4):471-481.  
[37] CRAIG A D,KHATTAK F,HASTIE P,et al.Xylanase and xylo-oligosaccharide prebiotic improve the growth performance and concentration of potentially prebiotic oligosaccharides in the ileum of broiler chickens[J].British Poultry Science,2020,61(1):70-78.  
[38] LEE S H,HOSSEINDOUST A,INGALE S L,et al.Thermostable xylanase derived from Trichoderma citrinoviride increases growth performance and non-starch polysaccharide degradation in broiler chickens[J].British Poultry Science,2020,61(1):57-62.  
[39] HU H,DAI S F,WEN A Y,et al.Efficient expression of xylanase by codon optimization and its effects on the growth performance and carcass characteristics of broiler[J].Animals,2019,9(2):65.
[40] ARCZEWSKA-WLOSEK A,SWIATKIEWICZ S,BEDERSKA-LOJEWSKA D,et al.The efficiency of xylanase in broiler chickens fed with increasing dietary levels of rye[J].Animals,2019,9(2):46.
[41] ZHANG S T,WANG C F,SUN Y,et al.Xylanase and fermented polysaccharide of Hericium caputmedusae reduce pathogenic infection of broilers by improving antioxidant and anti-inflammatory properties[J].Oxidative Medicine and Cellular Longevity,2018,2018:4296985.
[42] NOURMOHAMMADI R,KHOSRAVINIA H,AFZALI N.Effects of feed form and xylanase supplementation on metabolizable energy partitioning in broiler chicken fed wheat-based diets[J].Journal of Animal Physiology and Animal Nutrition,2018,102(6):1593-1600.  
[43] GONZALEZ-ORTIZ G,SOLA-ORIOL D,MARTINEZ-MORA M,et al.Response of broiler chickens fed wheat-based diets to xylanase supplementation[J].Poultry Science,2017,96(8):2776-2785.  
[44] AMERAH A M,ROMERO L F,AWATI A,et al.Effect of exogenous xylanase,amylase,and protease as single or combined activities on nutrient digestibility and growth performance of broilers fed corn/soy diets[J].Poultry Science,2017,96(4):807-816.  
[45] GONZÁLEZ-ORTIZ G,OLUKOSI O,BEDFORD M R.Evaluation of the effect of different wheats and xylanase supplementation on performance,nutrient and energy utilisation in broiler chicks[J].Animal Nutrition,2016,2(3):173-179.  
[46] LIU W C,KIM I H.Effects of dietary xylanase supplementation on performance and functional digestive parameters in broilers fed wheat-based diets[J].Poultry Science,2017,96(3):566-573.  
[47] ZHANG L,XU L,LEI L,et al.Effects of xylanase supplementation on growth performance,nutrient digestibility and non-starch polysaccharide degradation in different sections of the gastrointestinal tract of broilers fed wheat-based diets[J].Asian-Australasian Journal of Animal Sciences,2014,27(6):855-861.  
[48] 徐叶桐,曾志凯,何政肖,等.木聚糖酶对小麦型基础日粮肉仔鸡生长和消化率的影响[J].饲料研究,2017(7):1-4,11. XU Y T,ZENG Z K,HE Z X,et al.Effects of xylanase on growth performance and nutrient digestibility of broilers fed wheat-based diets[J].Feed Research,2017(7):1-4,11.(in Chinese)
[49] CHOCT M,KOCHER A,WATERS D L E,et al.A comparison of three xylanases on the nutritive value of two wheats for broiler chickens[J].British Journal of Nutrition,2004,92(1):53-61.  
[50] MASEY-O'NEILL H V,SINGH M,COWIESON A J.Effects of exogenous xylanase on performance,nutrient digestibility,volatile fatty acid production and digestive tract thermal profiles of broilers fed on wheat- or maize-based diet[J].British Poultry Science,2014,55(3):351-359.  
[51] STEFANELLO C,VIEIRA S L,CARVALHO P S,et al.Energy and nutrient utilization of broiler chickens fed corn-soybean meal and corn-based diets supplemented with xylanase[J].Poultry Science,2016,95(8):1881-1887.  
[52] GUO S S,LIU D,ZHAO X,et al.Xylanase supplementation of a wheat-based diet improved nutrient digestion and mRNA expression of intestinal nutrient transporters in broiler chickens infected with Clostridium perfringens[J].Poultry Science,2014,93(1):94-103.  
[53] ENGBERG R M,HEDEMANN M S,STEENFELDT S,et al.Influence of whole wheat and xylanase on broiler performance and microbial composition and activity in the digestive tract[J].Poultry Science,2004,83(6):925-938.  
[54] ALMIRALL M,FRANCESCH M,PEREZ-VENDRELL A M,et al.The differences in intestinal viscosity produced by barley and β-glucanase alter digesta enzyme activities and ileal nutrient digestibilities more in broiler chicks than in cocks[J].The Journal of Nutrition,1995,125(4):947-955.
[55] 黄婧溪,黄忠良,臧旭鹏,等.不同来源木聚糖酶及其组合对肉鸡肠道黏膜形态与二糖酶活性及其基因表达的影响[J].动物营养学报,2018,30(6):2271-2280. HUANG J X,LI Z L,ZANG X P,et al.Effects of different sources of xylanase and their combination on intestinal morphology,disaccharidase activities and gene expression of broilers[J].Chinese Journal of Animal Nutrition,2018,30(6):2271-2280.(in Chinese)
[56] 郭双双.木聚糖酶与益生菌缓解肠道炎症的作用及其机制[D].博士学位论文.北京:中国农业大学,2016. GUO S S,The attenuate effects of xylanase and probiotics on the intestinal inflammation and the related mechanisms[D].Ph.D.Thesis.Beijing:China Agricultural University,2016.(in Chinese)
[57] 王修启,张兆敏,张磊,等.日粮添加木聚糖酶对肉鸡小肠葡萄糖吸收及其转运载体基因表达影响[J].农业生物技术学报,2005,13(4):497-502. WANG X Q,ZHANG Z M,ZHANG L,et al.Dietary xylanase supplementation affects glucose absorption and SGLT1 mRNA expression in intestine of broiler chickens fed wheat-based diets[J].Journal of Agricultural Biotechnology,2005,13(4):497-502.(in Chinese)
[58] SIMON O.The mode of action of NSP hydrolysing enzymes in the gastrointestinal tract[J].Journal of Animal and Feed Sciences,1998,7(Suppl.1):115-123.
[59] WU Y B,RAVINDRAN V,THOMAS D G,et al.Influence of phytase and xylanase,individually or in combination,on performance,apparent metabolisable energy,digestive tract measurements and gut morphology in broilers fed wheat-based diets containing adequate level of phosphorus[J].British Poultry Science,2004,45(1):76-84.  
[60] WU Y B,RAVINDRAN V,THOMAS D G,et al.Influence of method of whole wheat inclusion and xylanase supplementation on the performance,apparent metabolisable energy,digestive tract measurements and gut morphology of broilers[J].British Poultry Science,2004,45(3):385-394.  
[61] BRENES A,SMITH M,GUENTER W,et al.Effect of enzyme supplementation on the performance and digestive tract size of broiler chickens fed wheat- and barley-based diets[J].Poultry Science,1993,72(9):1731-1739.  
[62] 崔朝霞.小麦饲料资源的开发利用-戊聚糖酶在肉仔鸡小麦日粮中应用的研究[D].硕士学位论文.郑州:河南农业大学,2002. CUI Z X.Development and utilization of wheat resources-a study on the effect of pentosanase supplementation to wheat based diets for broiler chickens[D].Master's Thesis.Zhengzhou:Henan Agricultural University,2002.(in Chinese)
[63] HVBENER K,VAHJEN W,SIMON O.Bacterial responses to different dietary cereal types and xylanase supplementation in the intestine of broiler chicken[J].Archiv für Tierernaehrung,2002,56(3):167-187.  
[64] BEDFORD M R,APAJALAHTI J.Microbial interactions in the response to exogenous enzyme utilization[M]//BEDFORD M R,PATRIDGE G G.Enzimes in farm animal nutrition.Oxon:CABI Publishing,2001:299-314.
[65] CHAPLA D,PANDIT P,SHAH A.Production of xylooligosaccharides from corncob xylan by fungal xylanase and their utilization by probiotics[J].Bioresource Technology,2012,115:215-221.
[66] LEE S A,APAJALAHTI J,VIENOLA K,et al.Age and dietary xylanase supplementation affects ileal sugar residues and short chain fatty acid concentration in the ileum and caecum of broiler chickens[J].Animal Feed Science and Technology,2017,234:29-42.
[67] NIAN F,GUO Y M,RU Y J,et al.Effect of xylanase supplementation on the net energy for production,performance and gut microflora of broilers fed corn/soy-based diet[J].Asian-Australasian Journal of Animal Sciences,2011,24(9):1282-1287.  
[68] MELO-DURÁN D,PÉREZ J F,GONZÁLEZ-ORTIZ G,et al.Influence of particle size and xylanase in corn-soybean pelleted diets on performance,nutrient utilization,microbiota and short-chain fatty acid production in young broilers[J].Animals,2020,10(10):1904.
[69] CHOCT M,SINLAE M,AI-JASSIM R A M,et al.Effects of xylanase supplementation on between-bird variation in energy metabolism and the number of Clostridium perfringens in broilers fed a wheat-based diet[J].Australian Journal of Agricultural Research,2006,57(9):1017-1021.  
[70] JOZEFIAK D,RUTKOWSKI A,JENSEN B B,et al.Effects of dietary inclusion of triticale,rye and wheat and xylanase supplementation on growth performance of broiler chickens and fermentation in the gastrointestinal tract[J].Animal Feed Science and Technology,2007,132(1/2):79-93.
[71] PAN D,YU Z T.Intestinal microbiome of poultry and its interaction with host and diet[J].Gut Microbes,2014,5(1):108-119.  
[72] VENEGAS D P,DE LA FUENTE M K,LANDSKRON G,et al.Short chain fatty acids (SCFAs)-mediated gut epithelial and immune regulation and its relevance for inflammatory bowel diseases[J].Frontiers in Immunology,2019,10:277.
[73] KUBIŚ M,KOŁODZIEJSKI P,PRUSZY Ń SKA-OSZMAŁEK E,et al.Emulsifier and xylanase can modulate the gut microbiota activity of broiler chickens[J].Animals,2020,12(10):1297.
[74] LAZARO R,GARCIA M,MEDEL P,et al.Influence of enzymes on performance and digestive parameters of broilers fed rye-based diets[J].Poultry Science,2003,82(1):132-140.  
[75] VON ENGELHARDT W,BARTELS J,KIRSCHBERGER S,et al.Role of short-chain fatty acids in the hind gut[J].The Veterinary Quarterly,1998,20(suppl.3):S52-S59.
[76] POUTEAU E,ROCHAT F,JANN A,et al.Chicory increases acetate turnover,but not propionate and butyrate peripheral turnovers in rats[J].British Journal of Nutrition,2008,99(2):287-296.  
[77] YANG Y,IJI P A,CHOCT M.Dietary modulation of gut microflora in broiler chickens:a review of the role of six kinds of alternatives to in-feed antibiotics[J].World's Poultry Science Journal,2009,65(1):97-114.  
[78] WANG Y Y,HENG C N,ZHOU X H,et al.Supplemental Bacillus subtilis DSM 29784 and enzymes,alone or in combination,as alternatives for antibiotics to improve growth performance,digestive enzyme activity,anti-oxidative status,immune response and the intestinal barrier of broiler chickens[J].The British Journal of Nutrition,2021,125(5):494-507.  
[79] CHEN Q H,LI M W,WANG X.Enzymology properties of two different xylanases and their impacts on growth performance and intestinal microflora of weaned piglets[J].Animal Nutrition,2016,2(1):18-23.  
[80] PETERSEN S T,WISEMAN J,BEDFORD M R.Effects of age and diet on the viscosity of intestinal contents in broiler chicks[J].British Poultry Science,1999,40(3):364-370.  
[81] BAUTIL A,VERSPREET J,BUYSE J,et al.Age-related arabinoxylan hydrolysis and fermentation in the gastrointestinal tract of broilers fed wheat-based diets[J].Poultry Science,2019,98(10):4606-4621.  
[82] GAO F,JIANG Y,ZHOU G H,et al.The effects of xylanase supplementation on performance,characteristics of the gastrointestinal tract,blood parameters and gut microflora in broilers fed on wheat-based diets[J].Animal Feed Science and Technology,2008,142(1/2):173-184.
[83] MENDES A R,RIBEIRO T,CORREIA B A,et al.Low doses of exogenous xylanase improve the nutritive value of triticale-based diets for broilers[J].Journal of Applied Poultry Research,2013,22(1):92-99.  
[84] FIGUEIREDO A A,CORREIA B A,RIBEIRO T,et al.The effects of restricting enzyme supplementation in wheat-based diets to broilers[J].Animal Feed Science and Technology,2012,172(3/4):194-200.
[85] DUSEL G,KLUGE H,JEROCH H,et al.Xylanase supplementation of wheat-based rations for broilers:influence of wheat characteristics[J].Journal of Applied Poultry Research,1998,7(2):119-131.  
[86] BEDFORD M R.The evolution and application of enzymes in the animal feed industry:the role of data interpretation[J].British Poultry Science,2018,59(5):486-493.  
[87] BAUTIL A,VERSPREET J,BUYSE J,et al.Arabinoxylan-oligosaccharides kick-start arabinoxylan digestion in the aging broiler[J].Poultry Science,2020,99(5):2555-2565.  
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