综述 REVIEW

支链氨基酸在畜禽低蛋白质饲粮体系中的应用

  • 简华锋 ,
  • 章旭春 ,
  • 董信阳 ,
  • 邹晓庭
展开
  • 1. 浙江大学动物科学学院饲料科学研究所, 生物饲料安全与污染防控国家工程实验室, 浙江大学动物分子营养学教育部重点实验室, 农业农村部(华东)动物营养与饲料重点实验室, 浙江省饲料与动物营养重点实验室, 杭州 310058;
    2. 湖州市安吉县科学技术局(安吉县技术创新服务中心), 湖州 313300
简华锋(1997-),男,贵州平塘人,硕士研究生,从事蛋鸡氨基酸营养研究。E-mail:jianhuafeng@zju.edu.cn

收稿日期: 2021-01-12

  网络出版日期: 2021-08-11

基金资助

国家蛋鸡产业技术体系(CARS-40-K10)

Application of Branched-Chain Amino Acids in Low-Protein Diet System for Livestock and Poultry

  • JIAN Huafeng ,
  • ZHANG Xuchun ,
  • DONG Xinyang ,
  • ZOU Xiaoting
Expand
  • 1. Key Laboratory of Animal Feed and Nutrition of Zhejiang Province, Key Laboratory of Animal Nutrition and Feed Science (Eastern of China), Ministry of Agriculture and Rural Affairs, Key Laboratory of Molecular Animal Nutrition (Zhejiang University), Ministry of Education, National Engineering Laboratory of Biological Feed Safety and Pollution Prevention and Control, Institute of Feed Science, College of Animal Science, Zhejiang University, Hangzhou 310058, China;
    2. Anji County Science and Technology Bureau in Huzhou City (Anji County Technology Innovation Service Center), Huzhou 313300, China

Received date: 2021-01-12

  Online published: 2021-08-11

Supported by

 

摘要

支链氨基酸(BCAA)包括亮氨酸(Leu)、异亮氨酸(Ile)和缬氨酸(Val)等必需氨基酸,具有调控机体糖和脂质代谢、蛋白质合成以及增强免疫等多种生物学功能。本文结合国内外的研究进展,对BCAA在机体内的吸收代谢、生物学功能及其在畜禽低蛋白质饲粮体系中的应用进行系统综述,重点阐述在低蛋白质饲粮中添加BCAA对畜禽生长和生产性能、蛋白质沉积和氮排泄、免疫功能以及肉品质的影响,以期为BCAA在畜禽低蛋白质饲粮体系中的研究和应用提供理论参考。

本文引用格式

简华锋 , 章旭春 , 董信阳 , 邹晓庭 . 支链氨基酸在畜禽低蛋白质饲粮体系中的应用[J]. 动物营养学报, 2021 , 33(8) : 4285 -4293 . DOI: 10.3969/j.issn.1006-267x.2021.08.009

Abstract

Branched-chain amino acids (BCAA) including essential amino acids such as leucine (Leu), isoleucine (Ile) and valine (Val), have various biological functions such as regulated body glucose and lipid metabolism, protein synthesis, and enhanced immune. This review systematically summarized the absorption and metabolism, biological functions of BCAA, and their application in low-protein diet system for livestock and poultry based on the research progress at home and abroad. We focused on the effects of adding BCAA to the low protein diet on the growth and production performance, protein deposition and nitrogen excretion, immune function and meat quality of livestock and poultry, which was expected to provide a theoretical reference for the research and application of BCAA in low-protein diet system for livestock and poultry.

参考文献

[1] BELL A W,BURHANS W S,OVERTON T R.Protein nutrition in late pregnancy,maternal protein reserves and lactation performance in dairy cows[J].Proceedings of the Nutrition Society,2000,59(1):119-126.  
[2] RECOULES E,SABBOH-JOURDAN H,NARCY A,et al.Exploring the in vivo digestion of plant proteins in broiler chickens[J].Poultry Science,2017,96(6):1735-1747.  
[3] AARNINK A J A,VERSTEGEN M W A.Nutrition,key factor to reduce environmental load from pig production[J].Livestock Science,2007,109(1/2/3):194-203.
[4] KERR B J,ZIEMER C J,TRABUE S L,et al.Manure composition of swine as affected by dietary protein and cellulose concentrations[J].Journal of Animal Science,2006,84(6):1584-1592.  
[5] HRISTOV A N,HANIGAN M,COLE A,et al.Ammonia emissions from dairy farms and beef feedlots:a review[J].Canadian Journal of Animal Science,2011,91(1):1-35.  
[6] WANG Y M,ZHOU J Y,WANG G,et al.Advances in low-protein diets for swine[J].Journal of Animal Science and Biotechnology,2018,9(4):60.
[7] 宋博,尹杰,郑昌炳,等.低蛋白质日粮在畜禽生产中的应用研究进展[J].中国饲料,2020(3):8-15. SONG B,YIN J,ZHENG C B,et al.Advances in application of low protein diets in livestock and poultry production[J].China Feed,2020(3):8-15.(in Chinese)
[8] National Research Council.Nutrient requirements of swine[M].10th ed.Washington,D.C.:National Academy Press,1998.
[9] 赵卿尧,顾宪红.低蛋白日粮应用前景与研究进展[J].家畜生态学报,2020,41(3):9-14. ZHAO Q Y,GU X H.Application prospect and research progress of low protein diet[J].Journal of Livestock Ecology,2020,41(3):9-14.(in Chinese)
[10] LOFTFIELD R B,HARRIS A.Participation of free amino acids in protein synthesis[J].Journal of Biological Chemistry,1956,219(1):151-159.  
[11] STOLL B,HENRY J,REEDS P J,et al.Catabolism dominates the first-pass intestinal metabolism of dietary essential amino acids in milk protein-fed piglets[J].The Journal of Nutrition,1998,128(3):606-614.  
[12] STOLL B,BURRIN D G.Measuring splanchnic amino acid metabolism in vivo using stable isotopic tracers[J].Journal of Animal Science,2006,84(Suppl.13):E60-E72.
[13] WU G,BAZER F W,DAI Z L,et al.Amino acid nutrition in animals:protein synthesis and beyond[J].Annual Review of Animal Biosciences,2014,2:387-417.
[14] WU G Y.Intestinal mucosal amino acid catabolism[J].The Journal of Nutrition,1998,128(8):1249-1252.  
[15] REEDS P J,BURRIN D G.Glutamine and the bowel[J].The Journal of Nutrition,2001,131(9):2505S-2508S.
[16] WANG B,WU G Y,ZHOU Z G,et al.Glutamine and intestinal barrier function[J].Amino Acids,2015,47(10):2143-2154.  
[17] JIAO N,WU Z L,JI Y,et al.L-glutamate enhances barrier and antioxidative functions in intestinal porcine epithelial cells[J].The Journal of Nutrition,2015,145(10):2258-2264.  
[18] DAI Z L,ZHANG J,WU G Y,et al.Utilization of amino acids by bacteria from the pig small intestine[J].Amino Acids,2010,39(5):1201-1215.  
[19] YANG Y X,DAI Z L,ZHU W Y.Important impacts of intestinal bacteria on utilization of dietary amino acids in pigs[J].Amino Acids,2014,46(11):2489-2501.  
[20] MORTENSEN P B,CLAUSEN M R.Short-chain fatty acids in the human colon:relation to gastrointestinal health and disease[J].Scandinavian Journal of Gastroenterology,1996,216:132-148.
[21] JASKIEWICZ J,ZHAO Y,HAWES J W,et al.Catabolism of isobutyrate by colonocytes[J].Archives of Biochemistry and Biophysics,1996,327(2):265-270.  
[22] HEIMANN E,NYMAN M,PÅLBRINK A K,et al.Branched short-chain fatty acids modulate glucose and lipid metabolism in primary adipocytes[J].Adipocyte,2016,5(4):359-368.  
[23] SURYAWAN A,HAWES J W,HARRIS R A,et al.A molecular model of human branched-chain amino acid metabolism[J].The American Journal of Clinical Nutrition,1998,68(1):72-81.  
[24] HUTSON S M,SWEATT A J,LANOUE K F.Branched-chain amino acid metabolism:implications for establishing safe intakes[J].The Journal of Nutrition,2005,135(6):1557S-1564S.
[25] LYNCH C J,ADAMS S H.Branched-chain amino acids in metabolic signalling and insulin resistance[J].Nature Reviews Endocrinology,2014,10(12):723-736.  
[26] 尹玉林,孙泽威.支链氨基酸调控机体糖代谢的研究进展[J].中国畜牧杂志,2019,55(5):11-14. YIN Y L,SUN Z W.Research progress on the regulation of glucose metabolism by branched-chain amino acids[J].Chinese Journal of Animal Science,2019,55(5):11-14.(in Chinese)
[27] ZHANG S H,ZENG X F,MAN R,et al.Novel metabolic and physiological functions of branched chain amino acids:a review[J].Journal of Animal Science and Biotechnology,2017,8:10.
[28] NISHITANI S,TAKEHANA K,FUJITANI S,et al.Branched-chain amino acids improve glucose metabolism in rats with liver cirrhosis[J].American Journal of Physiology:Gastrointestinal and Liver Physiology,2005,288(6):1292-1300.  
[29] ZHANG S H,YANG Q,REN M,et al.Effects of isoleucine on glucose uptake through the enhancement of muscular membrane concentrations of GLUT1 and GLUT4 and intestinal membrane concentrations of Na+/glucose co-transporter 1(SGLT-1) and GLUT2[J].British Journal of Nutrition,2016,116(4):593-602.  
[30] 陈嘉艺,周昕博,单安山,等.支链氨基酸对动物糖脂代谢的调控及作用机理[J].动物营养学报,2020,32(9):4078-4085. CHEN J Y,ZHOU X B,SHAN A S,et al.Regulation and mechanism of branched-chain amino accession animal glycolipid metabolism[J].Journal of Animal Nutrition,2020,32(9):4078-4085.(in Chinese)
[31] GUO F F,CAVENER D R.The GCN2 eIF2α kinase regulates fatty-acid homeostasis in the liver during deprivation of an essential amino acid[J].Cell Metabolism,2007,5(2):103-114.  
[32] CHENG Y,MENG Q S,WANG C X,et al.Leucine deprivation decreases fat mass by stimulation of lipolysis in white adipose tissue and upregulation of uncoupling protein 1(UCP1) in brown adipose tissue[J].Diabetes,2010,59(1):17-25.  
[33] YING C,QIAN Z,QING S M,et al.Leucine deprivation stimulates fat loss via increasing CRH expression in the hypothalamus and activating the sympathetic nervous system[J].Molecular Endocrinology,2011,25(9):1624-1635.  
[34] NIE C X,HE T,ZHANG W J,et al.Branched-chain amino acids:beyond nutrition metabolism[J].International Journal of Molecular Sciences,2018,19(4):954-963.  
[35] WILKINSON D J,HOSSAIN T,HILL D S,et al.Effects of leucine and its metabolite β-hydroxy-β-methylbutyrate on human skeletal muscle protein metabolism[J].The Journal of Physiology,2013,591(11):2911-2923.  
[36] KIMBALL S R,JEFFERSON L S.Signaling pathways and molecular mechanisms through which branched-chain amino acids mediate translational control of protein synthesis[J].Journal of Nutrition,2006,136(Suppl.1):227S-231S.
[37] CALDER P C.Branched-chain amino acids and immunity[J].Journal of Nutrition,2006,136(Suppl.1):288S-293S.
[38] 常银莲.支链氨基酸对肉鸡小肠发育及TOR通路相关基因表达的影响[D].硕士学位论文.北京:中国农业科学院,2016. CHANG Y L.Effects of BCAAs on small intestinal development and relative genes expression of TOR pathway in broilers[D].Master's Thesis.Beijing:Chinese Academy of Agricultural Sciences,2016.(in Chinese)
[39] HARPER A E,MILLER R H,BLOCK K P.Branched-chain amino acid metabolism[J].Annual Review of Nutrition,1984,4:409-454.
[40] 段叶辉.低蛋白日粮平衡BCAAs比例调控10-30 kg猪肌肉组织蛋白质与脂质代谢的机制研究[D].博士学位论文.长沙:中国科学院大学,2017. DUAN Y H.Effects of low-protein diets with optimal branched-chain amino acid ratio on protein meta bolism and lipid metabolism in skeletal muscle of 10-30 kg pigs[D].Ph.D. Thesis.Changsha:University of Chinese Academy of Sciences,2017.(in Chinese)
[41] ZHENG L F,WEI H K,CHENG C S,et al.Supplementation of branched-chain amino acids to a reduced-protein diet improves growth performance in piglets:involvement of increased feed intake and direct muscle growth-promoting effect[J].British Journal of Nutrition,2016,115(12):2236-2245.  
[42] DUAN Y H,ZENG L M,LI F N,et al.Effects of dietary branched-chain amino acid ratio on growth performance and serum amino acid pool of growing pigs[J].Journal of Animal Science,2016,94(Suppl 3):129-134.
[43] LORDELO M M,GASPAR A M,LE BELLEGO L,et al.Isoleucine and valine supplementation of a low-protein corn-wheat-soybean meal-based diet for piglets:growth performance and nitrogen balance[J].Journal of Animal Science,2008,86(11):2936-2941.  
[44] 刘尧君,任曼,曾祥芳,等.低氮日粮补充支链氨基酸提高断奶仔猪生长性能和氮的利用效率[J].中国畜牧杂志,2014,50(7):44-47. LIU X J,REN M,ZENG X F,et al.Low protein diet supplemented with branched chain amino acids improves growth performance and nitrogen utilization in weaned piglet[J].Chinese Journal of Animal Science,2014,50(7):44-47.(in Chinese)
[45] OSPINA-ROJAS I C,MURAKAMI A E,FANHANI J C,et al.Tryptophan,threonine and isoleucine supplementation in lowprotein diets for commercial laying hens[J].Semina:Ciencias Agrarias,2015,36(3):1735-1743.  
[46] WAGUESPACK A M,POWELL S,BIDNER T D,et al.Effect of incremental levels of L-lysine and determination of the limiting amino acids in low crude protein corn-soybean meal diets for broilers[J].Poultry Science,2009,88(6):1216-1226.  
[47] DO NASCIMENTO G R,MURAKAMI A E,OSPINA-ROJAS I C,et al.Digestible valine requirements in low-protein diets for broilers chicks[J].Brazilian Journal of Poultry Science,2016,18(3):381-386.  
[48] OSPINA-ROJAS I C,MURAKAMI A E,DUARTE C R A,et al.Leucine and valine supplementation of low-protein diets for broiler chickens from 21 to 42 days of age[J].Poultry Science,2016,96(4):914-922.
[49] OSPINA-ROJAS I C,MURAKAMIA E,DUARTE C R A,et al.Valine,isoleucine,arginine and glycine supplementation of low-protein diets for broiler chickens during the starter and grower phases[J].British Poultry Science,2014,55(6):766-773.  
[50] ALAGAWANY M,EL-HACK M E A,LAUDADIO V,et al.Effect of low-protein diets with crystalline amino acid supplementation on egg production,blood parameters and nitrogen balance in laying Japanese quails[J].Avian Biology Research,2014,7(4):235-243.  
[51] 陈将,刘国华,PIRZADO S A,等.低蛋白质饲粮补充缬氨酸对肉鸡生长性能、屠宰性能和血清指标的影响[J].动物营养学报,2019,31(4):1604-1612. CHEN J,LIU G H,PIRZADO S A,et al.Effects of valine supplementation in low-protein diets on growth performance,slaughter performance and serum indices of broilers[J].Journal of Animal Nutrition,2019,31(4):1604-1612.(in Chinese)
[52] 张宇峰,田敏,恒景会,等.支链氨基酸对断奶仔猪肠道和肌肉氨基酸转运载体表达的影响[J].中国畜牧杂志,2020,56(4):92-99. ZHANG Y F,TIAN M,HENG J H,et al.Effects of branched-chain amino acids on intestinal and muscular amino acid transporter expression of weaning piglets[J].Chinese Journal of Animal Science,2020,56(4):92-99.(in Chinese)
[53] ZHANG S H,QIAO S Y,REN M,et al.Supplementation with branched-chain amino acids to a low-protein diet regulates intestinal expression of amino acid and peptide transporters in weanling pigs[J].Amino Acids,2013,45(5):1191-1205.  
[54] ZHANG S H,REN M,ZENG X F,et al.Leucine stimulates ASCT2 amino acid transporter expression in porcine jejunal epithelial cell line (IPEC-J2) through PI3K/Akt/mTOR and ERK signaling pathways[J].Amino Acids,2014,46(12):2633-2642.  
[55] ZHENG L F,ZUO F R,ZHAO S J,et al.Dietary supplementation of branched-chain amino acids increases muscle net amino acid fluxes through elevating their substrate availability and intramuscular catabolism in young pigs[J].British Journal of Nutrition,2017,117(7):911-922.  
[56] ZHENG L F,WEI H K,HE P L,et al.Effects of supplementation of branched-chain amino acids to reduced-protein diet on skeletal muscle protein synthesis and degradation in the fed and fasted states in a piglet model[J].Nutrients,2016,9(1):17.
[57] YIN Y L,YAO K,LIU Z J,et al.Supplementing L-leucine to a low-protein diet increases tissue protein synthesis in weanling pigs[J].Amino Acids,2010,39(5):1477-1486.  
[58] DOS SANTOS G C,GARCIA E A,VIEIRA J A,et al.Performance of Japanese quails fed diets with low-protein and isoleucine[J].Acta Scientiarum.Animal Sciences,2016,38(2):219-231.
[59] 董小英,李均祥,任晓强,等.支链氨基酸影响猪免疫功能的研究进展[J].中国饲料,2016(16):10-13,17. DONG X Y,LI J X,REN X Q,et al.Research progress on the influence of branched chain amino acids on pig immune function[J].China Feed,2016,16(3):10-13,17.(in Chinese)
[60] 任曼.支链氨基酸调控仔猪肠道防御素表达和免疫屏障功能的研究[D].博士学位论文.北京:中国农业大学,2014. REN M.Branched-chain amino acids modulate intestinal defensin expression and immune barrier function of piglets[D].Ph.D. Thesis.Beijing:China Agricultural University,2014.(in Chinese)
[61] REN M,ZHANG S H,ZENG X F,et al.Branched-chain amino acids are beneficial to maintain growth performance and intestinal immune-related function in weaned piglets fed protein restricted diet[J].Asian-Australasian Journal of Animal Sciences,2015,28(12):2236-2244.
[62] REN M,LIU C,ZENG X F,et al.Amino acids modulates the intestinal proteome associated with immune and stress response in weaning pig[J].Molecular Biology Reports,2014,41(6):3611-3620.  
[63] REN M,LIU X T,WANG X,et al.Increased levels of standardized ileal digestible threonine attenuate intestinal damage and immune responses in Escherichia coli K88+ challenged weaned piglets[J].Animal Feed Science and Technology,2014,195:67-75.
[64] MAO X B,LI M L,TANG J,et al.Dietary leucine supplementation improves the mucin production in the jejunal mucosa of the weaned pigs challenged by porcine rotavirus[J].PLoS One,2015,10(9):e0137380.
[65] DUAN Y H,LI F N,GUO Q P,et al.Branched-chain amino acid ratios modulate lipid metabolism in adipose tissues of growing pigs[J].Journal of Functional Foods,2018,40:614-624.
[66] DUAN Y H,DUAN Y M,LI F N,et al.Effects of supplementation with branched-chain amino acids to low-protein diets on expression of genes related to lipid metabolism in skeletal muscle of growing pigs[J].Amino Acids,2016,48(9):2131-2144.  
[67] 李凤娜,尹杰,段叶辉,等.猪低蛋白日粮的作用效应与技术实践进展[J].农业现代化研究,2018,39(6):961-969. LI F N,YIN J,DUAN Y H,et al.Effect and technologic practicing progress of low-protein diet in pig[J].Research of Agricultural Modernization,2018,39(6):961-969.(in Chinese)
文章导航

/