猪营养与饲料 SWINE NUTRITION AND FEED

白藜芦醇对生长肥育期宁乡猪肉品质的影响

  • 段平男 ,
  • 杨婷 ,
  • 陈佳亿 ,
  • 秦昆鹏 ,
  • 赵玉蓉
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  • 湖南农业大学动物科学技术学院, 长沙 410128
段平男(1996-),男,湖南衡阳人,硕士研究生,动物营养与饲料科学专业。E-mail:401334287@qq.com

收稿日期: 2020-11-13

  网络出版日期: 2021-08-11

基金资助

湖南省教育厅重点项目(18A087)

Effects of Resveratrol on Meat Quality of Growing and Fattening Ningxiang Pigs

  • DUAN Pingnan ,
  • YANG Ting ,
  • CHEN Jiayi ,
  • QIN Kunpeng ,
  • ZHAO Yurong
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  • College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China

Received date: 2020-11-13

  Online published: 2021-08-11

Supported by

 

摘要

本试验旨在研究饲粮中添加白藜芦醇对生长肥育期宁乡猪肉品质的影响。选用体重(43±1) kg的宁乡猪24头,随机分为2个组,每组6个重复,每个重复2头(公母各占1/2)。对照(CON)组饲喂基础饲粮,白藜芦醇(RES)组饲喂在基础饲粮中添加300 mg/kg白藜芦醇的试验饲粮。试验期80 d。饲养结束后,每栏取1头体重接近平均体重的猪进行屠宰,并采集背最长肌、股二头肌及腰大肌样品,用于肉品质指标检测。结果表明:1)饲粮中添加白藜芦醇对宁乡猪的生长性能和肌肉主要营养成分含量均无显著影响(P>0.05)。2)与对照组相比,白藜芦醇组宁乡猪宰后24 h背最长肌的红度(a*)显著提高(P<0.05),宰后24 h背最长肌和股二头肌的黄度(b*)有降低趋势(0.05≤P<0.10)。3)与对照组相比,白藜芦醇组宁乡猪背最长肌的磷酸果糖激酶(PFK)活性极显著降低(P<0.01),股二头肌的丙酮酸激酶(PK)活性和乳酸(LA)含量显著降低(P<0.05);肌肉pH无显著变化(P>0.05)。4)与对照组相比,白藜芦醇组宁乡猪背最长肌的谷胱甘肽过氧化物酶(GSH-Px)活性、总抗氧化能力(T-AOC)及股二头肌的GSH-Px活性均极显著提高(P<0.01),股二头肌的丙二醛(MDA)含量极显著降低(P<0.01)。由此可见,饲粮中添加白藜芦醇可以提高宁乡猪肌肉的抗氧化能力,降低宰后肌肉的糖酵解速率,对肉品质具有一定的改善作用。

本文引用格式

段平男 , 杨婷 , 陈佳亿 , 秦昆鹏 , 赵玉蓉 . 白藜芦醇对生长肥育期宁乡猪肉品质的影响[J]. 动物营养学报, 2021 , 33(8) : 4364 -4372 . DOI: 10.3969/j.issn.1006-267x.2021.08.017

Abstract

The purpose of this experiment was to study the effects of dietary resveratrol on meat quality of growing and fattening Ningxiang pigs. Twenty-four Ningxiang pigs with body weight (43±1) kg were randomly divided into 2 groups with 6 replicates per group and 2 pigs per replicate (males and females in half). Pigs were fed a basal diet in control (CON) group, and the others in resveratrol (RES) group were fed the basal diets supplemented with 300 mg/kg resveratrol. The trial lasted for 80 days. After the end of experiment, 1 head of Ningxiang pig which body weight was similar with average body weight was selected for slaughter determination, and samples of longissimus dorsi, Biceps femoris and Psoas major were collected for meat quality analysis. The results showed as follows:1) dietary resveratrol had no significant effects on growth performance and the contents of muscles main nutrients of Ningxiang pigs (P>0.05). 2) Compared with CON group, the redness (a*) of longissimus dorsi at 24 h after slaughter of Ningxiang pigs in RES group was significantly increased (P<0.05), while the yellowness (b*) of Longissimus dorsi and Biceps femoris at 24 h after slaughter had a decreasing tendency (0.05 ≤ P<0.10). 3) Compared with CON group, the activity of phosphofructokinase (PFK) of Longissimus dorsi of Ningxiang pigs in RES group was significantly decreased (P<0.01), the activity of pyruvate kinase (PK) and the content of lactic acid (LA) of Biceps femoris in RES group were significantly decreased (P<0.05). While the pH of muscle among groups had no significant difference (P>0.05). 4) Compared with CON group, the activity of glutathione peroxidase (GSH-Px) and total antioxidant capacity (T-AOC) of longissimus dorsi and the activity of GSH-Px of Biceps femoris of Ningxiang pigs in RES group were significantly increased (P<0.01), and the content of malondialdehyde (MDA) of Biceps femoris was significantly decreased (P<0.01). Consequently, dietary resveratrol can improve muscle antioxidant capacity, and reduce postmortem muscle glycolysis rate, thus improve meat quality of Ningxiang pigs.

参考文献

[1] 肖林霞,卢其能,李润根.白藜芦醇的研究进展[J].现代农业科技,2015(24):264-265. XIAO L X,LU Q N,LI R G.Research progress of resveratrol[J].Modern Agricultural Science and Technology,2015(24):264-265.(in Chinese)
[2] 夏琴.基于脂联素信号通路研究白藜芦醇调控猪肉品质和骨骼肌肌纤维类型转化的作用机制[D].硕士学位论文.南宁:广西大学,2019. XIA Q.The regulation mechanism of resveratrol on pork quality and muscle fiber type transformation of skeletal muscle was studied based on adiponectin signaling pathway[D].Master's Thesis.Nanning:Guangxi University,2019.(in Chinese)
[3] ZHANG C,LUO J Q,YU B,et al.Dietary resveratrol supplementation improves meat quality of finishing pigs through changing muscle fiber characteristics and antioxidative status[J].Meat Science,2015,102:15-21.
[4] 张彩云,康相涛.白藜芦醇对肉仔鸡抗氧化能力和肉品质的影响[J].江苏农业学报,2011,27(3):587-591. ZHANG C Y,KANG X T.Effects of dietary resveratrol on antioxidant ability and meat quality in broilers[J].Jiangsu Journal of Agricultural Sciences,2011,27(3):587-591.(in Chinese)
[5] 中华人民共和国农业部.畜禽肉质的测定:NY/T 1333-2007[S].北京:中国农业出版社,2007. Ministry of Agriculture of the People's Republic of China.Determination of meat quality of livestock and poultry:NY/T 1333-2007[S].Beijing:China Agriculture Press,2007.(in Chinese)
[6] DASZKIEWICZ T,BAK T,DENABURSKI J.Quality of pork with a different intramuscular fat (IMF) content[J].Polish Journal of Food and Nutrition Sciences,2005,14(1):31-35.
[7] MANCINI R A,HUNT M C.Current research in meat color[J].Meat Science,2005,71(1):100-121.  
[8] 戴四发,闻爱友,王立克,等.肉仔鸡肌肉色泽与品质相关性研究[J].中国农业大学学报,2007,12(1):61-64. DAI S F,WEN A Y,WANG L K,et al.Study on relationship between meat color and meat quality of Arber Acres broiler[J].Journal of China Agricultural University,2007,12(1):61-64.(in Chinese)
[9] 袁艳枝,邓文,金瑶瑶,等.猪肉品质评定指标及影响因素的研究进展[J].黑龙江畜牧兽医,2020(1):31-35,40. YUAN Y Z,DENG W,JIN Y Y,et al.Research progress of pork quality evaluation index and its influencing factors[J].Heilongjiang Animal Science and Veterinary Medicine,2020(1):31-35,40.(in Chinese)
[10] 程天赋,俞龙浩.宰后糖酵解对肉品质影响的研究进展[J].食品研究与开发,2017,38(15):219-224. CHENG T F,YU L H.Research progress on effects of postmortem glycolysis on meat quality[J].Food Research and Development,2017,38(15):219-224.(in Chinese)
[11] HOU X H,LIU Q F,MENG Q S,et al.TMT-based quantitative proteomic analysis of porcine muscle associated with postmortem meat quality[J].Food Chemistry,2020,328:127-133.
[12] 李朋颖.猪TIGAR转录水平与宰后糖酵解关系研究[D].硕士学位论文.北京:中国农业科学院,2014. LI P Y.Study on the relationship between TIGAR transcription level and postmortem glycolysis in pigs[D].Master's Thesis.Beijing:Chinese Academy of Agricultural Sciences,2014.(in Chinese)
[13] GOMEZ L S,ZANCAN P,MARCONDES M C,et al.Resveratrol decreases breast cancer cell viability and glucose metabolism by inhibiting 6-phosphofructo-1-kinase[J].Biochimie,2013,95(6):1336-1343.  
[14] 肖忠盛,龙泓,丁成明,等.白藜芦醇通过调控mTOR信号通路抑制结直肠癌细胞的增殖和糖酵解作用[J].实用医学杂志,2019,35(14):2210-2214. XIAO Z C,LONG H,DING C M,et al.Resveratrol inhibits the proliferation and glycolysis of colorectal cancer cells by regulating the mTOR signaling pathway[J].The Journal of Practical Medicine,2019,35(14):2210-2214.(in Chinese)
[15] ZHANG C,WANG L,ZHAO X H,et al.Dietary resveratrol supplementation prevents transport-stress-impaired meat quality of broilers through maintaining muscle energy metabolism and antioxidant status[J].Poultry Science,2017,96(7):2219-2225.  
[16] 胡瑶莲,张恒志,陈代文,等.白藜芦醇对生长育肥猪抗氧化能力、空肠黏膜免疫及结肠菌群的影响[J].动物营养学报,2019,31(1):459-468. HU Y L,ZHANG H Z,CHEN D W,et al.Effects of resveratrol on antioxidant capacity,jejunal mucosal immunity and colonic microflora of growing-finishing pigs[J].Chinese Journal of Animal Nutrition,2019,31(1):459-468.(in Chinese)
[17] MENG Q W,GUO T,LI G Q,et al.Dietary resveratrol improves antioxidant status of sows and piglets and regulates antioxidant gene expression in placenta by Keap1-Nrf2 pathway and Sirt1[J].Journal of Animal Science and Biotechnology,2018,9:34.
[18] 李华,曾勇庆,魏述东,等.猪宰后肌肉SOD与MDA的变化及其对肉质特性的影响[J].畜牧兽医学报,2010,41(3):257-261. LI H,ZENG Y Q,WEI S D,et al.Changes of superoxide dismutase activity and malondialdehyde level in postmortem muscle and their association with meat quality in pigs[J].Journal of Animal Husbandry and Veterinary Medicine,2010,41(3):257-261.(in Chinese)
[19] 蒋红琴.番茄红素对巴美肉羊肉品质的影响及其抗氧化机理研究[D].博士学位论文.北京:中国农业大学,2015. JIANG H Q.Study on the effect of lycopene on the meat quality of Pamir lamb and its anti-oxidation mechanism[D].Ph.D.Thesis.Beijing:China Agricultural University,2015.(in Chinese)
[20] 吴成帆.牛肉肌红蛋白分离纯化及脂质氧化对其稳定性的影响[D].硕士学位论文.兰州:甘肃农业大学,2016. WU C F.Effects of separation and purification of beef myoglobin and lipid oxidation on its stability[D].Master's Thesis.Lanzhou:Gansu Agricultural University,2016.(in Chinese)
[21] 赵晓惠.白藜芦醇和L-茶氨酸对肉鸡生长性能和肉品质的影响及其机理初探[D].硕士学位论文.合肥:安徽农业大学,2019. ZHAO X H.Effect of resveratrol and L-theanine on growth performance and meat quality of broilers and its mechanism[D].Master's Thesis.Hefei:Anhui Agricultural University,2019.(in Chinese)
[22] ZHANG C,YANG L,ZHAO X H,et al.Effect of dietary resveratrol supplementation on meat quality,muscle antioxidative capacity and mitochondrial biogenesis of broilers[J].Journal of the Science of Food and Agriculture,2018,98(3):1216-1221.  
[23] MENG Q W,SUN S S,BAI Y S,et al.Effects of dietary resveratrol supplementation in sows on antioxidative status,myofiber characteristic and meat quality of offspring[J].Meat Science,2020,167:108176.
[24] ARORA T,SINGH S,SHARMA R K.Probiotics:interaction with gut microbiome and antiobesity potential[J].Nutrition,2013,29(4):591-596.  
[25] CHAMBAZ A,SCHEEDER M R L,KREUZER M,et al.Meat quality of Angus,Simmental,Charolais and Limousin steers compared at the same intramuscular fat content[J].Meat Science,2003,63(4):491-500.  
[26] 张宏博.宰前因素和宰后处理对商品猪生长发育、屠宰及胴体质量和猪肉品质影响的研究[D].博士学位论文.呼和浩特:内蒙古农业大学,2015. ZHANG H B.Effects of premortem factors and postmortem treatment on growth,slaughter,carcass quality and pork quality of commercial pigs[D].Ph.D.Thesis.Hohhot:Inner Mongolia Agricultural University,2015.(in Chinese)
[27] 张振玲.日本商品猪腰肉系水力与肌内脂肪含量的关系[J].猪业科学,2018,35(10):24-25. ZHANG Z L.The relationship between water power and intramuscular fat content in Japanese commercial pork loin[J].Swine Industry Science,2018,35(10):24-25.(in Chinese)
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