动物营养学报 >

2021 , Vol. 33 >Issue 8: 4373 - 4383

DOI: https://doi.org/10.3969/j.issn.1006-267x.2021.08.018

猪营养与饲料 SWINE NUTRITION AND FEED

饲用凝结芽孢杆菌对感染产肠毒素大肠杆菌K88断奶仔猪生长性能和肠道健康的影响

  • 柳成东 ,
  • 肖明霞 ,
  • 赵平 ,
  • 李琦华 ,
  • 白华毅 ,
  • 程志斌
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  • 云南农业大学动物科学技术学院, 昆明 650201
柳成东(1996-),男,云南曲靖人,硕士研究生,研究方向为单胃动物营养与饲料科学。E-mail:1024364616@qq.com

收稿日期: 2021-01-20

  网络出版日期: 2021-08-11

基金资助

国家自然科学基金项目（31860664）；云南省农业联合面上项目（2017FG001-067）；云南省创新团队项目（2017HC030）

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Effect of Dietary Bacillus coagulans on Growth Performance and Intestinal Health of Weaned Piglets Challenged with Enterotoxigenic Escherichia coli K88

  • LIU Chengdong ,
  • XIAO Mingxia ,
  • ZHAO Ping ,
  • LI Qihua ,
  • BAI Huayi ,
  • CHENG Zhibin
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  • College of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, China

Received date: 2021-01-20

  Online published: 2021-08-11

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摘要

本文旨在研究饲用凝结芽孢杆菌对感染产肠毒素大肠杆菌（ETEC） K88断奶仔猪生长性能和肠道菌群、形态及细胞因子表达的影响。试验选用32头25日龄断奶的健康"杜×长×大"商品公猪，按照初始体重一致原则分成4个组，每组8个重复，单笼饲养。ETEC K88感染前7 d，2个组饲喂基础饲粮（BD组），另外2个组饲喂在基础饲粮中添加1.0×107 CFU/g凝结芽孢杆菌的饲粮（PD组）。第8天起，开展2×2双因素试验，4个组设计如下：1） NCH+BD组，口服生理盐水、饲喂基础饲粮；2） NCH+PD组，口服生理盐水、饲喂添加凝结芽孢杆菌饲粮；3） CH+BD组，口服ETEC K88、饲喂基础饲粮；4） CH+PD组，口服ETEC K88、饲喂添加凝结芽孢杆菌饲粮。试验猪口服5 mL ETEC K88（活菌数1.0×109 CFU/mL）或5 mL 0.9%生理盐水，持续3 d。试验用凝结芽孢杆菌是经体外牛津杯法确定的1株对ETEC K88具有良好抑菌效果的菌株，添加量1.0×107 CFU/g。试验第11天，取仔猪空肠、回肠、盲肠及食糜样本，测定肠道菌群、形态及细胞因子表达量。结果表明：1） ETEC K88感染处理显著降低断奶仔猪平均日增重和平均日采食量、空肠和盲肠食糜中乳酸杆菌数量、空肠和回肠绒毛高度以及空肠黏膜白细胞介素-10（IL-10） mRNA表达量（P<0.05）；同时，显著提高断奶仔猪料重比和腹泻指数、空肠和盲肠食糜中大肠杆菌数量、空肠和回肠隐窝深度以及空肠黏膜白细胞介素-6（IL-6） mRNA表达量（P<0.05）。2）饲用凝结芽孢杆菌处理显著提高断奶仔猪平均日增重和平均日采食量、空肠和盲肠食糜中乳酸杆菌数量、空肠和回肠绒毛高度以及空肠黏膜IL-10 mRNA表达量（P<0.05）；同时，显著降低断奶仔猪料重比和腹泻指数、空肠和盲肠食糜中大肠杆菌数量、空肠和回肠隐窝深度以及空肠黏膜IL-6 mRNA表达量（P<0.05）。3）除腹泻指数外，ETEC K88感染处理与饲用凝结芽孢杆菌处理之间对其他指标均无显著互作效应（P>0.05）。结果提示，无论是否ETEC K88感染处理，饲用凝结芽孢杆菌均可通过平衡肠道菌群、维持肠道正常形态和缓解肠道炎症反应，改善断奶仔猪生长性能。

关键词： 凝结芽孢杆菌; 产肠毒素大肠杆菌K88感染; 断奶仔猪; 生长性能; 肠道健康

本文引用格式

柳成东 , 肖明霞 , 赵平 , 李琦华 , 白华毅 , 程志斌 . 饲用凝结芽孢杆菌对感染产肠毒素大肠杆菌K88断奶仔猪生长性能和肠道健康的影响[J]. 动物营养学报, 2021 , 33(8) : 4373 -4383 . DOI: 10.3969/j.issn.1006-267x.2021.08.018

Abstract

The purpose of this paper was to study the effects of dietary Bacillus coagulans on the growth performance, intestinal flora, morphology and cytokine expression of weaned piglets with enterotoxigenic Escherichia coli (ETEC) K88 challenge. Thirty-two healthy Duroc×Landrace×Yorkshire commercial boars weaned at 25 days of age were divided into 4 groups according to the body weight with 8 replicates per group and fed in single cages. Seven days before ETEC K88 infection, the piglets in two groups were fed a basal diet (BD group), and the piglets in the other two groups were fed the basal diet supplemented with 1.0×107 CFU/g Bacillus coagulans (PD group). From day 8, a 2×2 two-factor experiment was carried out, and the design of the four groups was as follows:1) NCH+BD group was given normal saline orally and fed the basal diet; 2) NCH+PD group was given normal saline orally and fed the Bacillus coagulans supplementation diet; 3) CH+BD group was given ETEC K88 orally and fed the basal diet; 4) CH+PD group was given ETEC K88 orally and fed the Bacillus coagulans supplementation diet. Pigs were given 5 mL ETEC K88 (viable count was 1.0×109 CFU/mL) or 5 mL 0.9% normal saline orally for 3 days. The Bacillus coagulans used in this experiment was a strain with good antibacterial effects on ETEC K88, which was determined by Oxford cup method in vitro, and the supplemental level was 1.0×107 CFU/g. On day 11 of the experiment, the samples of jejunum, ileum, cecum and their ingesta of piglets were taken to determine intestinal flora, morphology and cytokine expression levels. The results showed as follows:1) ETEC K88 infection significantly decreased the average daily gain and average daily feed intake, Lactobacillus number in jejunal and cecal ingesta, villus height in jejunum and ileum, and interleukin-10 (IL-10) mRNA expression level in jejunal mucosa of weaned piglets (P<0.05), while significantly increased the ratio of feed to gain, diarrhea index, Escherichia coli number in jejunal and cecal ingesta, crypt depth in jejunum and ileum, and interleukin-6 (IL-6) mRNA expression level in jejunal mucosa of weaned piglets (P<0.05). 2) Dietary Bacillus cloagulans significantly increased the average daily gain and average daily feed intake, Lactobacillus number of in jejunal and cecal ingesta, villus height in jejunum and ileum, and IL-10 mRNA expression level in jejunal mucosa of weaned piglets (P<0.05), while significantly decreased the ratio of feed to gain, diarrhea index, Escherichia coli number in jejunal and cecal ingesta, crypt depth in jejunum and ileum and IL-6 mRNA expression level in jejunal mucosa of weaned piglets (P<0.05). 3) There was no significant interaction effect between ETEC K88 infection and dietary Bacillus coagulans treatment on the other indices except diarrhea index (P>0.05). In conclusion, dietary Bacillus coagulans can improve the growth performance of weaned piglets by balancing intestinal flora, maintaining normal intestinal morphology and relieving intestinal inflammation regardless of the ETEC K88 challenge.

Key words： Bacillus coagulans; enterotoxigenic Escherichia coli K88 challenge; weaned piglets; growth performance; intestinal health

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