动物营养学报 >

2021 , Vol. 33 >Issue 8: 4405 - 4414

DOI: https://doi.org/10.3969/j.issn.1006-267x.2021.08.021

禽营养与饲料 POULTRY NUTRITION AND FEED

天然叶黄素替代阿朴酯对黄羽肉鸡不同部位皮肤着色的影响

  • 王一冰 ,
  • 张盛 ,
  • 李辉 ,
  • 叶金玲 ,
  • 范秋丽 ,
  • 林厦菁 ,
  • 苟钟勇 ,
  • 蒋守群
展开
  • 1. 广东省农业科学院动物科学研究所, 畜禽育种国家重点实验室, 农业农村部华南动物营养与饲料重点实验室, 广东省畜禽育种与营养研究重点实验室, 广州 510640;
    2. 广州立达尔生物科技股份有限公司, 广州 510663
王一冰(1990-),女,山东青岛人,副研究员,博士,从事黄羽肉鸡营养与免疫研究。E-mail:wangyibing77@163.com

收稿日期: 2021-01-26

  网络出版日期: 2021-08-11

基金资助

国家肉鸡产业技术体系项目（CARS-41-G10）；国家自然科学基金青年基金项目（31802104）；广东省科技计划项目（2017B020202003，2019A050505007）；广州市科技计划重点项目（201804020091）；广东省农业科学院院长基金项目（201908）；科技创新战略专项（高水平农科院建设）（R2019PY-QF008）

收起

Effects of Natural Lutein Instead of Apolylate on Pigmenting of Skins in Different Parts of Yellow-Feathered Chicken

  • WANG Yibing ,
  • ZHANG Sheng ,
  • LI Hui ,
  • YE Jinling ,
  • FAN Qiuli ,
  • LIN Xiajing ,
  • GOU Zhongyong ,
  • JIANG Shouqun
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  • 1. Guangdong Provincial Key Laboratory of Animal Breeding and Nutrition, Key Laboratory of Animal Nutrition and Feed Science in South China, Ministry of Agriculture and Rural Affairs, State Key Laboratory of Livestock and Poultry Breeding, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China;
    2. Guangzhou Leader Bio-Technology Co., Ltd., Guangzhou 510663, China

Received date: 2021-01-26

  Online published: 2021-08-11

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摘要

本研究旨在探究天然叶黄素替代阿朴酯对黄羽肉鸡不同部位皮肤着色的影响。试验采用单因子试验设计，按照体重均一原则选用600只35日龄快大型黄羽肉鸡作为试验鸡。试验分3组，对照组（斑螯黄质组）在基础饲粮中添加50 mg/kg的斑螯黄，各试验组分别在基础饲粮中添加200 mg/kg阿朴酯与50 mg/kg的斑螯黄（阿朴酯组）、200 mg/kg叶黄素与50 mg/kg的斑螯黄（叶黄素组），每组5个重复，每个重复40只鸡，饲养周期35 d。结果表明：与对照组相比，1）各试验组35~70日龄肉鸡的生长性能均没有显著差异（P>0.05）；2）阿朴酯组肉鸡试验第21、28天及宰后第3、5天的背部皮红度（a*）与黄度（b*）值均显著提高（P<0.05），叶黄素组肉鸡试验第35天、宰后第1天的背部皮肤a*值和宰后第1~5天的b*值显著提高（P<0.05），第14天亮度（L*）值显著降低（P<0.05）；3）对于腹部，阿朴酯组肉鸡在第21、28天及宰后第3、5天皮肤a*与b*值显著提高（P<0.05），叶黄素组肉鸡在第21天腹部皮肤a*值和第21~35天、宰后第5天皮肤b*值显著提高（P<0.05），阿朴酯组和叶黄素组第28天腹部皮肤的L*值均显著降低（P<0.05）；4）阿朴酯组和叶黄素组肉鸡第14、21天胫部皮肤的a*值与第14~28天及宰后第1天的b*值显著提高（P<0.05），第14、21、35天胫部皮肤的L*值显著降低（P<0.05）；阿朴酯与叶黄素组肉鸡第14、21、35天胫部皮肤颜色（罗氏比色扇数值）均显著增加（P<0.05），且叶黄素组肉鸡在宰后第1~5天胫部皮肤颜色也显著增加（P<0.05）；5）阿朴酯组与叶黄素组黄羽肉鸡腹脂的a*与b*值均显著提高（P<0.05）；6）阿朴酯组与叶黄素组肾脏中β胡萝卜素加氧酶1（BCO1）基因相对表达量显著增加（P<0.05）；叶黄素组β胡萝卜素加氧酶2（BCO2）基因相对表达量显著高于其余2组（P<0.05）。综上所述，相较于单独添加斑螯黄质，饲粮中阿朴酯或天然叶黄素与斑螯黄复配均可上调叶黄素沉积效应基因BCO1基因相对表达量，提高肉鸡皮肤的a*与b*值，降低L*值，促进着色，且二者作用效果相近。天然叶黄素可以替代阿朴酯用于黄羽肉鸡皮肤着色。

关键词： 天然叶黄素; 肉鸡; 皮肤; 着色; 阿朴酯

本文引用格式

王一冰 , 张盛 , 李辉 , 叶金玲 , 范秋丽 , 林厦菁 , 苟钟勇 , 蒋守群 . 天然叶黄素替代阿朴酯对黄羽肉鸡不同部位皮肤着色的影响[J]. 动物营养学报, 2021 , 33(8) : 4405 -4414 . DOI: 10.3969/j.issn.1006-267x.2021.08.021

Abstract

This experiment was conducted to investigate the effects of natural lutein instead of apolylate on pigmenting of skins in yellow-feathered chicken. Six hundred 35-d yellow-feathered chicken were fed with basal diets supplemented with 50 mg/kg chrysanthin (control group, chrysanthin group), 200 mg/kg apolylate and 50 mg/kg chrysanthin (apolylate group), or 200 mg/kg lutein and 50 mg/kg chrysanthin (lutein group) for 5 weeks, with five replicates per group and 40 chicken per replicate. The results showed that, compared with the control group, 1) there were no effects (P>0.05) on growth performance of broilers aged 35 to 70 days; 2) the a* and b* values in apolylate group at the 21st and 28th day of experiment, the 3rd and 5th day postmortem were significantly increased (P<0.05), the a* value in lutein group at the 35th day of experiment and 1rd day postmortem, and the b* value at 1rd to 5st day postmortem were significantly increased (P<0.05), also the L* value in lutein group was significantly decreased (P<0.05) at the 14th day of experiment; 3) for abdominal skin, the a* and b* values in apolylate group was significantly increased (P<0.05) at the 21st and 28th day of experiment and the 3rd and 5th day postmortem, the a* value at 21st day of experiment and the b* value at the 21st to 35th day of experiment and the 5th day postmortem, the L* value in apolylate group and lutein group at 28th day of experiment also were significantly decreased (P<0.05); 4) for the tibial skin, the a* value at the 14th and 21th day, and b* value at 14th to 28th day of experiment and 1st day postmortem in apolylate group and lutein group was both significantly increased (P<0.05), and the L* value at 14th, 21st and 35th day of experiment was significantly decreased (P<0.05); the tibial skin color (Roche scale) in apolylate group and lutein group was significantly increased (P<0.05) at the 14th, 21st, and 35th day of experiment, furthermore, the tibial skin color of broilers in lutein group was significantly increased from 1 to 5 days after slaughter (P<0.05); 5) the a* and b* values of abdominal fat of chicken in apolylate group and lutein group were significantly increased (P<0.05); 6) the relative expression level of β carotene oxygenase 1 (BCO1) gene in kidney in apolylate group and lutein group was significantly increased (P<0.05), and relative expression level of β carotene oxygenase 2 (BCO2) gene in lutein group was significantly higher than that in other groups (P<0.05). In conclusion, compared with the supplementation of chrysanthin only, the supplementation of chrysanthin with apolylate or chrysanthin with lutein up-regulate the relative expression level of BCO1 gene in kidney, increase the a* and b* values and reduce the L* value of skin in chicken, and chrysanthin with apolylate or natural lutein show the same effects. Natural lutein is able to replace apocynate for pigmenting of skins in yellow-feathered chicken.

Key words： natural lutein; yellow-feathered chicken; skin; coloring; apocynate

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