反刍与草食动物营养与饲料 RUMINANT AND HERBIVORE NUTRITION AND FEED

牛至精油对平凉红牛生长性能、血液生理指标、肉品质及肌肉脂肪酸的影响

  • 李佳龙 ,
  • 张瑞 ,
  • 吴建平 ,
  • 张科 ,
  • 豆思远 ,
  • 曾金焱 ,
  • 刘婷 ,
  • 宫旭胤 ,
  • 王建福 ,
  • 雷赵民
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  • 1. 甘肃农业大学动物科学技术学院, 兰州 730070;
    2. 甘肃省农业科学院, 兰州 730070;
    3. 西北农林科技大学动物科技学院, 杨凌 712100;
    4. 甘肃省动物疫病预防控制中心, 兰州 730046;
    5. 甘肃农业职业技术学院, 兰州 730020;
    6. 甘肃省农业科学院畜草与绿色农业研究所, 兰州 730070
李佳龙(1995-),男,甘肃定西人,硕士研究生,畜牧专业。E-mail:957561925@qq.com

收稿日期: 2021-01-29

  网络出版日期: 2021-08-11

基金资助

甘肃省高等学校产业支撑技术项目"平凉红牛种养结合高效循环生产体系建设技术应用"(2020C-08);甘肃省农业农村厅现代丝路寒旱农业"草食畜种养结合循环生产体系建设技术研究与应用"专项(GSSLCSX-2020-1);甘肃省科技重大专项计划"肉牛高效生态营养技术体系研究与示范"(17ZD2NC020);农业部公益性行业(农业)科研专项"北方作物秸秆饲用化利用技术研究与示范"(201503134);兰州市科技局重点项目"玉米秸秆饲料化关键技术集成与示范推广"(2012-2-159)

Effects of Oregano Essential Oil on Growth Performance, Blood Physiological Indices, Meat Quality and Muscle Fatty Acids of Pingliang Red Cattle

  • LI Jialong ,
  • ZHANG Rui ,
  • WU Jianping ,
  • ZAHNG Ke ,
  • DOU Siyuan ,
  • ZENG Jinyan ,
  • LIU Ting ,
  • GONG Xuyin ,
  • WANG Jianfu ,
  • LEI Zhaomin
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  • 1. College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China;
    2. Gansu Academy of Agricultural Sciences, Lanzhou 730070, China;
    3. College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China;
    4. Animal Disease Prevention and Control Centre of Gansu Province, Lanzhou 730046, China;
    5. Gansu Agricultural Vocational and Technical College, Lanzhou 730020, China;
    6. Animal Husbandry, Pasture and Green Agriculture Institute, Gansu Academy of Agricultural Science, Lanzhou 730070, China

Received date: 2021-01-29

  Online published: 2021-08-11

Supported by

 

摘要

本试验旨在研究牛至精油对平凉红牛生长性能、血液生理指标、肉品质及肌肉脂肪酸的影响。选取12月龄左右、体重为(270.74±26.24) kg的健康平凉红牛(阉牛)9头,随机分为3组(每组3头),对照组(CON组)饲喂基础饲粮,试验组分别在基础饲粮中添加10(L组)、20 g/(d·头)(H组)牛至精油。饲喂584 d (预试期14 d,正试期570 d)后屠宰。结果表明:1)试验组的终末体重、平均日增重、宰前活重、净肉重、眼肌面积显著高于CON组(P<0.05),L组的皮下脂肪厚度和H组的胴体胸深显著高于CON组(P<0.05),且L组和H组的平均日增重较CON组分别提高了16.07%、21.43%。2)试验组的白细胞计数、淋巴细胞计数、单核细胞计数、粒细胞计数、血红蛋白浓度显著高于CON组(P<0.05),L组的血小板计数、血小板压积显著高于CON组(P<0.05)。3)试验组的肌肉pH45 min、pH24 h、剪切力显著低于CON组(P<0.05);L组的大理石花纹评分和H组的熟肉率显著高于CON组(P<0.05),H组的蒸煮损失显著低于CON组(P<0.05)。4)对于肌肉脂肪酸组成和含量,试验组饱和脂肪酸含量显著低于CON组(P<0.05),不饱和脂肪酸含量显著高于CON组(P<0.05);CON组和L组的C8 : 0、C13 : 0、C20 : 2含量显著低于H组(P<0.05),试验组的C18 : 0和C18 : 1n9c含量显著高于CON组(P<0.05);n-3多不饱和脂肪酸、n-6多不饱和脂肪酸、多不饱和脂肪酸/饱和脂肪酸值各组间差异均不显著(P>0.05),但L组和H组的多不饱和脂肪酸/饱和脂肪酸值较CON组分别提高了16.67%和33.33%。综上所述,饲粮中添加牛至精油可以提高平凉红牛的生长性能及产肉量,改善牛肉品质,改变肌肉脂肪酸的组成和含量,提升机体的健康状态。

本文引用格式

李佳龙 , 张瑞 , 吴建平 , 张科 , 豆思远 , 曾金焱 , 刘婷 , 宫旭胤 , 王建福 , 雷赵民 . 牛至精油对平凉红牛生长性能、血液生理指标、肉品质及肌肉脂肪酸的影响[J]. 动物营养学报, 2021 , 33(8) : 4478 -4490 . DOI: 10.3969/j.issn.1006-267x.2021.08.028

Abstract

This experiment was conducted to study the effects of oregano essential oil on growth performance, blood physiological indices, meat quality and muscle fatty acids of Pingliang red cattle. A total of 9 healthy Pingliang red cattle (steers) about 12 months of age and weighing (270.74±26.24) kg were randomly divided into 3 groups (3 heads per group). The cattle in control group (CON group) were fed a basal diet, and the cattle in experimental groups were fed the basal diet supplemented with 10 (L group) and 20 g/(d·head) (H group) oregano essential oil, respectively. The animals were fed for 584 days (pre-trial period was 14 days, and trial period was 570 days). The results showed as follows:1) the final weight and average daily gain (ADG), live weight before slaughter, meat weight and eye muscle area of the experimental groups were significantly higher than those of CON group (P<0.05), the thickness of subcutaneous fat of L group and the carcass chest depth of H group were significantly higher than those of CON group (P<0.05), the ADG of L group and H group was 16.07% and 21.43% higher than that of CON group. 2) The white blood cell (WBC) count, lymphocyte (LYM) count, monocyte (MON) count, granulocyte (GRA) count and hemoglobin (HGB) concentration of experimental groups were significantly higher than those of CON group (P<0.05), and the platelet (PLT) count and plateletcrit (PCT) of L group were significantly higher than those of CON group (P<0.05). 3) The muscle pH45 min, pH24 h and shear force were significantly lower than those of CON group (P<0.05); the marbling score of L group and the cooked meat rate of H group were significantly higher than those of CON group (P<0.05), and the cooking loss of H group was significantly lower than that of CON group (P<0.05). 4) For the composition and content of fatty acids in muscle, the content of saturated fatty acid (SFA) of experimental groups was significantly lower than that of CON group (P<0.05), and the content of unsaturated fatty acid (UFA) was significantly higher than that of CON group (P<0.05); the contents of C8:0, C13:0 and C20:2 of CON group and L group were significantly lower than those of H group (P<0.05), and the contents of C18:0 and C18:1n9c of experimental groups were significantly higher than those of CON group (P<0.05); there were no significant differences in n-3 polyunsaturated fatty acid (PUFA), n-6PUFA, PUFA/SFA (P/S) value among groups (P>0.05), but the P/S value of L group and H group were 16.67% and 33.33% higher than those of CON group. In conclusion, adding oregano essential oil into the diet can improve the growth performance and meat production of Pingliang red cattle, also can improve the quality of beef, change the composition and contents of fatty acids in muscle, and improve the health of the body.

参考文献

[1] JUKES T H,STOKE L R,TALOE R R,et al.Growth-promoting effect of aureomycin on pigs[J].Archives of Biochemistry,1950,26(2):324-325.
[2] 王云鹏,马越.养殖业抗生素的使用及其潜在危害[J].中国抗生素杂志,2008,33(9):519-523. WANG Y P,MA Y.Potential public hazard of using antibiotics in livestock industry[J].Chinese Journal of Antibiotics,2008,33(9):519-523.(in Chinese)
[3] ZHANG L,GU J,WANG X J,et al.Fate of antibiotic resistance genes and mobile genetic elements during anaerobic co-digestion of Chinese medicinal herbal residues and swine manure[J].Bioresource Technology,2018,250:799-805.
[4] 柏妍.牛至精油替代莫能菌素对荷斯坦奶公牛瘤胃功能和生长性能的影响[D].博士学位论文.兰州:甘肃农业大学,2020. BAI Y.Effects of oregano essential oil as potential alternative for monensin on ruminal funtion and growth performance of young Holstein dairy bulls[D].Ph.D.Thesis.Lanzhou:Gansu Agricultural University,2020.(in Chinese)
[5] 周瑞.牛至精油对羔羊胃肠道结构和功能及其微生物多样性的影响[D].博士学位论文.兰州:甘肃农业大学,2019. ZHOU R.The effects of oregano essential oil on gastrointestinal tract structure,function and microbiota diversity of lambs[D].Ph.D.Thesis.Lanzhou:Gansu Agricultural University,2019.(in Chinese)
[6] KHATTAK F,RONCHI A,CASTELLI P,et al.Effects of natural blend of essential oil on growth performance,blood biochemistry,cecal morphology,and carcass quality of broiler chickens[J].Poultry Science,2014,93(1):132-137.  
[7] HERAWATI O,UNTARI T,ANGGITA M,et al.Effect of mangosteen (Garcinia mangostana L.) peel extract as an antibiotic growth promoter on growth performance and antibiotic resistance in broilers[J].Veterinary World,2019,13(4):796-800.
[8] 刘艳,黄红燕,李金荣,等.天然植物"抗生素"牛至资源及繁育研究进展[J].中国野生植物资源,2020,39(9):51-56. LIU Y,HUANG H Y,LI J R,et al.research progress in resources and breeding of Origanum vulgare L.,a natural "antibiotic" species[J].Chinese Wild Plant Resources,2020,39(9):51-56.(in Chinese)
[9] 邓奇风,刘志强,范觉鑫,等.牛至油的研究进展及其在猪生产中的应用[J].中国猪业,2018,13(2):41-46. DENG Q F,LIU Z Q,FAN J X,et al.Research progress of oregano oil and its application in pig production[J].China Swine Industry,2018,13(2):41-46.(in Chinese)
[10] 牛彪,梁剑平,刘宇.牛至精油在兽药基础研究中的进展[J].动物医学进展,2018,39(4):92-96. NIU B,LIANG J P,LIU Y.Progress on oregano essential oil in basic veterinary drug research[J].Progress in Veterinary Medicine,2018,39(4):92-96.(in Chinese)
[11] 杨昭,刘婷,吴建平,等.TMR中添加牛至精油抑菌作用的研究[J].中国农学通报,2015,31(14):8-13. YANG Z,LIU T,WU J P,et al.Bacteriostatic action of oregano essential oil adding to TMR[J].Chinese Agricultural Science Bulletin,2015,31(14):8-13.(in Chinese)
[12] 徐方华.牛至油及主要成分对瘤胃发酵特性和甲烷产量影响的研究[D].硕士学位论文.延吉:延边大学,2014. XU F H.Research on the effect of oregano oil and main components on rumen fermentation characteristics and methane production[D].Master's Thesis.Yanji:Yanbian University,2014.(in Chinese)
[13] 刘婷,张瑞,吴建平,等.牛至精油结合不同包装方式对冷鲜羊肉保鲜效果的影响[J].食品与发酵工业,2020,46(4):139-145. LIU T,ZHANG R,WU J P,et al.Effect of oregano essential oil combined with packaging methods on preservation of chilled mutton[J].Food and Fermentation Industries,2020,46(4):139-145.(in Chinese)
[14] 陈昊,刘婷,吴建平,等.牛至精油对新生犊牛生长发育和血液免疫的影响[J].草业科学,2017,34(10):2141-2148. CHEN H,LIU T,WU J P,et al.Effects of oregano essential oil on growth and hematogenic immunity of newborn calves[J].Pratacultural Science,2017,34(10):2141-2148.(in Chinese)
[15] 陈昊,刘婷,姚喜喜,等.牛至精油对荷斯坦奶牛乳房炎和腹泻发病率的影响[J].中国草食动物科学,2015,35(2):39-41. CHEN H,LIU T,YAO X X,et al.Effect of oregano essential oil on the mastitis and diarrhea of Holstein cows[J].Chinese Herbivore Science,2015,35(2):39-41.(in Chinese)
[16] 姜辉,雷赵民,焦婷,等.日粮中添加牛至油对河西绒山羊育肥性能的影响研究[J].草业学报,2018,27(11):142-149. JIANG H,LEI Z M,JIAO T,et al.Effects of dietry oregano oil on growth and carcass traits of Hexi cashmere goats[J].Acta Prataculturae Sinica,2018,27(11):142-149.(in Chinese)
[17] 韦胜,郎侠,王彩莲,等.牛至精油对绵羊生长性能、屠宰性能及肉品质的影响[J].动物营养学报,2019,31(6):2823-2829. WEI S,LANG X,WANG C L,et al.Effects of oregano essential oil on growth performance,slaughter performance and meat quality of sheep[J].Chinese Journal of Animal Nutrition,2019,31(6):2823-2829.(in Chinese)
[18] LEI Z M,ZHANG K,LI C,et al.Ruminal metagenomic analyses of goat data reveals potential functional microbiota by supplementation with essential oil-cobalt complexes[J].BMC Microbiology,2019,19:30.
[19] 昝林森.牛生产学[M].2版.北京:中国农业出版社,2007. ZAN L S.Cattle production science[M].2rd ed.Beijing:China Agricuture University Press,2007.(in Chinese)
[20] 刘婷,吴建平,宫旭胤,等.牛至精油对甘肃高山细毛羊肉品质、脂肪酸组成及含量和抗氧化性能的影响[J].食品与发酵工业,2020,46(9):164-170. LIU T,WU J P,GONG X Y,et al.Effect of oregano essential oil on meat quality,fatty acid profiles and antioxidant capacity of Gansu alpine fine-wool sheep[J].Food and fermentation industries,2020,46(9):164-170.(in Chinese)
[21] LIU T,LEI Z M,WU J P,et al.Fatty acid composition differences between adipose depot sites in dairy and beef steer breeds[J].Food Science and Technology,2015,52(3):1656-1662.
[22] 梁建勇,李晓康,焦婷,等.牛至精油在畜禽生产中的研究进展[J].中国饲料,2017(9):22-25. LIANG J Y,LI X K,JIAO T,et al.Research progress of oregano essential oil in livestock and poultry production[J].China Feed,2017(9):22-25.(in Chinese)
[23] GHAZI S,AMJADIAN T,NOROUZI S.Single and combined effects of vitamin C and oregano essential oil in diet,on growth performance,and blood parameters of broiler chicks reared under heat stress condition[J].International Journal of Biometeorology,2015,59(8):1019-1024.  
[24] PASSARINHO A T P,DIAS N F,CAMILLOTO G P,et al.Sliced bread preservation through oregano essential oil-containing sachet[J].Journal of Food Process Engineering,2014,37(1):53-62.  
[25] 姚喜喜,吴建平,刘婷,等.全混合日粮中添加牛至精油对泌乳期荷斯坦奶牛生产性能和蹄病发生率的影响[J].草业科学,2016,33(2):299-304. YAO X X,WU J P,LIU T,et al.Effects of adding the oregano essential oil to the total mixed ration on production performance and incidence of hoof disease of lactating Holstein dairy cows[J].Pratacultural science,2016,33(2):299-304.(in Chinese)
[26] 孔繁云,吴建平,张科,等.饲粮中添加牛至精油、罗伊乳杆菌和莫能菌素对绵羊生长性能、屠宰性能、胴体性状、肉质性状及血清抗氧化指标的影响[J].动物营养学报,2020,32(8):3771-3779. KONG F Y,WU J P,ZHANG K,et al.Effects of dietary oregano essential oil,lactobacillus reuteri and monensin on growth performance,slaughter performance,carcass traits and serum antioxidant indices of sheep[J].Chinese Journal of Animal Nutrition,2020,32(8):3771-3779.(in Chinese)
[27] 王雅静.糖萜素、牛至油对育肥后期肉牛作用效果的研究[D].硕士学位论文.重庆:西南大学,2007. WANG Y J.A study on the Etticacy of saccherpenin and oregano oil supplement in late-finishing cattle[D].Master's Thesis.Chongqing:Southwest University,2007.(in Chinese)
[28] 白云鹏,张瑞,吴建平,等.牛至精油对平凉红牛生长性能、屠宰性能及肉品质的影响[J].动物营养学报,2020,32(12):5778-5787. BAI Y P,ZHANG R,WU J P,et al.Effects of oregano essential oil on growth performance,slaughter performance and meat quality of Pingliang red cattle[J].Chinese Journal of Animal Nutrition,2020,32(12):5778-5787.(in Chinese)
[29] 张娇娇,闫琦,刘培培,等.日粮中添加不同比例的缓释尿素对后备奶牛血液生理指标的影响[J].家畜生态学报,2018,39(2):29-33. ZHANG J J,YAN Q,LIU P P,et al.Effect of adding different proportions of slow-release urea in the diet on blood physiological indexes of dairy heifer[J].Acta Ecologiae Animalis Domastici,2018,39(2):29-33.(in Chinese)
[30] 刘培培.早期断奶措施对母牦牛和犊牦牛的影响[D].硕士学位论文.兰州:兰州大学,2017. LIU P P.Effects of early weaning strategies on yak cows and yak calves[D].Master's Thesis.Lanzhou:Lanzhou University,2017.(in Chinese)
[31] 王娟.添加牛至精油与有机钴协同(EOC)对河西绒山羊生长代谢及消化道组织结构的影响[D].硕士学位论文.兰州:甘肃农业大学,2018. WANG J.Effect of EOC on growth metabolism and digestive tract structure of Hexi cashmere goat[D].Master's Thesis.Lanzhou:Gansu Agricultural University,2018.(in Chinese)
[32] 柏妍,郎侠,王彩莲,等.饲粮中添加牛至精油和莫能菌素对荷斯坦犊牛血清生化指标、消化酶活性及瘤胃微生物区系的影响[J].畜牧兽医学报,2019,50(12):2458-2469. BAI Y,LANG X,WANG C L,et al.Effects of oregano essential oil and monensin in diet on serum biochemical parameters,digestive enzyme activities and ruminal microflora of Holstein calves[J].Chinese Journal of Animal and Veterinary Sciences,2019,50(12):2458-2469.(in Chinese)
[33] 周光宏.畜产品加工学[M].2版.北京:中国农业出版社,2011:60-61. ZHOU G H,Animal products processing[M].2rd ed.Beijing:China Agricuture Press,2011:60-61.(in Chinese)
[34] GIANNENAS I,FLOROU-PANERI P,PAPAZAHARIADOU M,et al.Effect of dietary supplementation with oregano essential oil on performance of broilers after experimental infection with Eimeria tenella[J].Archives of Animal Nutrition,2003,57(2):99-106.  
[35] ASIMWE L,KIMAMBO A E,LASWAI G H,et al.Effect of days in feedlot on growth performance,carcass and meat quality attributes of Tanzania shorthorn zebu steers[J].Tropical Animal Health and Production,2015,47(5):867-876.  
[36] BIDANEL J P,CARITEZ J C,GRUAND J,et al.Growth,carcass and meat quality performance of crossbred pigs with graded proportions of Meishan genes[J].Genetics Selection Evolution,1993,25:83.
[37] LI L Y,ZHU Y K,WANG X Y,et al.Effects of different dietary energy and protein levels and sex on growth performance,carcass characteristics and meat quality of F1 Angus×Chinese Xiangxi yellow cattle[J].Journal of Animal Science and Biotechnology,2014,5:21.
[38] 杨明,龙虎,文勇立,等.四川牦牛、黄牛不同品种肌肉脂肪酸组成的气相色谱-质谱分析[J].食品科学,2008,29(3):444-449. YANG M,LONG H,WEN Y L,et al.Analysis of muscle fatty acid composition of different breed yaks and yellow cattles in Sichuan by GC-MS[J].Food Science,2008,29(3):444-449.(in Chinese)
[39] SIERRA V,ALDAI N,CASTRO P,et al.Prediction of the fatty acid composition of beef by near infrared transmittance spectroscopy[J].Meat Science,2007,78(3):248-255.
[40] 孙晓青,毛祝新,傅华,等.牧草中脂肪酸及其影响因素[J].草业科学,2014,31(9):1774-1780. SUN X Q,MAO Z X,FU H,et al.Fatty acid characteristics of forage and its influence factors[J].Pratacultural Science,2014,31(9):1774-1780.(in Chinese)
[41] 李文博,罗玉龙,刘畅,等.饲养方式对苏尼特羊肉挥发性风味成分和脂肪酸组成的影响[J].食品科学,2019,40(24):207-213. LI W P,LUO Y L,LIU C,et al.Effects of feeding patterns on volatile flavor components and fatty acid composition of Sunit sheep meat[J].Food Seience,2019,40(24):207-213.(in Chinese)
[42] MUKHERJEE A,KENNY H A,LENGYEL E.Unsaturated fatty acids maintain cancer cell stemness[J].Cell Stem Cell,2017,20(3):291-292.  
[43] ARSHAD M S,SOHAIB M,AHMAD R S,et al.Ruminant meat flavor influenced by different factors with special reference to fatty acids[J].Lipids in Health and Disease,2018,17:223.
[44] 段淇斌,张长庆,赵冬青,等.谷饲育肥对牛肉中脂肪酸组成及含量的影响[J].中国牛业科学,2018,44(1):4-7. DUAN Q B,ZHANG C Q,ZHAO D Q,et al.Effects of fattening technology with cereals on fatty acid composition and content in beef[J].Chinese Cattle Science,2018,44(1):4-7.(in Chinese)
[45] 张伟敏,钟耕,王炜.单不饱和脂肪酸营养及其生理功能研究概况[J].粮食与油脂,2005,19(3):13-15. ZHANG W M,ZHONG G,WANG W.Study survey of nutrition and biological function of MUFA[J].Cereals & Oils,2005,19(3):13-15.(in Chinese)
[46] CARLSON S J,O'LOUGHLIN A A,ANEZ-BUSTILLOS L,et al.A diet with docosahexaenoic and arachidonic acids as the sole source of polyunsaturated fatty acids is sufficient to support visual,cognitive,motor,and social development in mice[J].Frontiers in Neuroscience,2019,13:72.
[47] 刘立山,周瑞,吴建平,等.日粮中添加牛至精油对牛肉脂肪酸以及血液生化指标的影响[J].中国饲料,2019(13):47-51. LIU L S,ZHOU R,WU J P,et al.Effects of fatty acids in beef and blood biochemical index by adding oregano oil into ration[J].China Feed,2019(13):47-51.(in Chinese)
[48] 陈银基,鞠兴荣,周光宏.饱和脂肪酸分类与生理功能[J].中国油脂,2008,33(3):35-39. CHEN Y J,JU X R,ZHOU G H.Classification and physiological function of saturated fatty acids[J].China Oils and Fats,2008,33(3):35-39.(in Chinese)
[49] COLOMBO S M,PARRISH C C,WIJEKOON M P A.Optimizing long chain-polyunsaturated fatty acid synthesis in salmonids by balancing dietary inputs[J].PLoS One,2018,13(10):e0205347.
[50] 祝仁铸.野莱F1猪肉品质及肌内脂肪沉积机理的研究[D].硕士学位论文.泰安:山东农业大学,2013. ZHU R Z.Study on the meat qualities and the sedimentary mechanism of intramuscular fat in YL F1 pigs[D].Master's Thesis.Tai'an:Shandong Agricultural University,2013.(in Chinese)
[51] 夏广军,严昌国,刘成名.混合植物油对肉牛血液、肌肉中十八碳脂肪酸及肌肉中氨基酸含量的影响[J].饲料工业,2012,33(23):58-60. XIA G J,YAN C G,LIU C M.Effects of mixed vegetable oil on the contents of octadecanoic fatty acids and amino acids in blood,muscle of beef cattle[J].Feed Industry,2012,33(23):58-60.(in Chinese)
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