水产动物营养与饲料 AQUATIC ANIMAL NUTRITION AND FEED

金针菇菇头对草鱼生长、消化酶活性、肠道组织结构、肌肉品质及血清生化指标的影响

  • 杨琳霖 ,
  • 胡毅 ,
  • 朱波 ,
  • 钟蕾 ,
  • 魏泽宏 ,
  • 唐涛 ,
  • 张俊智
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  • 1. 湖南农业大学动物科学技术学院, 湖南省特色水产资源利用工程技术研究中心, 长沙 410128;
    2. 省部共建淡水鱼类发育生物学国家重点实验室, 湖南师范大学生命科学学院, 长沙 410081
杨琳霖(1997-),女,湖南株洲人,硕士研究生,研究方向为水产动物营养与饲料。E-mail:1085544200@qq.com

收稿日期: 2021-01-18

  网络出版日期: 2021-08-11

基金资助

水产养殖动物新型蛋白源开发与高效饲料研制项目(2019YFD0900200);省部共建淡水鱼类发育生物学国家重点实验室开放课题基金(2019KF003)

Effects of Flammulina velutipes Stembase on Growth, Digestive Enzyme Activities, Intestinal Tissue Structure, Muscle Quality and Serum Biochemical Parameters of Grass Carp (Ctenopharyngodon idella)

  • YANG Linlin ,
  • HU Yi ,
  • ZHU Bo ,
  • ZHONG Lei ,
  • WEI Zehong ,
  • TANG Tao ,
  • ZHANG Junzhi
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  • 1. Hunan Engineering Research Center for Utilization of Characteristics of Resources, College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China;
    2. State Key Laboratory of Developmental Biology of Freshwater Fish, College of Life Sciences, Hunan Normal University, Changsha 410081, China

Received date: 2021-01-18

  Online published: 2021-08-11

Supported by

 

摘要

本试验主要研究饲料中添加金针菇菇头对草鱼生长、消化酶活性、肠道组织结构、肌肉品质及血清生化指标的影响,为合理开发金针菇菇头、减少生物资源浪费、配制绿色优质草鱼饲料提供试验依据。挑选360尾初始体重为(20.00±0.25) g的草鱼,随机分成3组,每组3个重复,每个重复40尾。各组饲喂基础饲料中分别添加0(对照组)、0.5%(Ⅰ组)和1.0%(Ⅱ组)的金针菇菇头的3种等氮等脂试验饲料。试验期8周。结果表明:1)与对照组相比,Ⅰ组和Ⅱ组的增重率显著增加(P<0.05),Ⅰ组的肠体比显著降低(P<0.05),Ⅱ组的肝胰腺指数显著升高(P<0.05)。各组之间粗脂肪、粗灰分、粗蛋白质和水分含量无显著差异(P>0.05)。2)与对照组相比,Ⅱ组的肠道脂肪酶活性显著升高(P<0.05),Ⅰ组和Ⅱ组的肠道淀粉酶活性显著降低(P<0.05)。3)与对照组相比,Ⅰ组和Ⅱ组的中肠肌层厚度和杯状细胞数量显著增加(P<0.05)。4)与对照组相比,Ⅰ组的肌肉硬度、胶黏性和咀嚼性显著升高(P<0.05)。5)与对照组相比,Ⅰ组和Ⅱ组的血清葡萄糖含量、谷草转氨酶活性显著升高(P<0.05),Ⅰ组和Ⅱ组的血清尿素氮、甘油三酯含量和谷丙转氨酶活性显著下降(P<0.05),Ⅱ组的血清总胆固醇含量显著下降(P<0.05)。综上所述,饲料中添加适量金针菇菇头可促进草鱼生长,改善肠道组织结构,提高肠道消化酶活性,改善草鱼肌肉品质。

本文引用格式

杨琳霖 , 胡毅 , 朱波 , 钟蕾 , 魏泽宏 , 唐涛 , 张俊智 . 金针菇菇头对草鱼生长、消化酶活性、肠道组织结构、肌肉品质及血清生化指标的影响[J]. 动物营养学报, 2021 , 33(8) : 4569 -4579 . DOI: 10.3969/j.issn.1006-267x.2021.08.037

Abstract

This experiment was conducted to study the effects of dietary Flammulina velutipes stembase on growth, digestive enzyme activities, intestinal tissue structure, muscle quality and serum biochemical parameters of grass carp (Ctenopharyngodon idella), and to provide a theory for rationally exploiting the Flammulina velutipes stembase, reducing the waste of biological resources and producing green and high quality grass carp diet. A total of 360 grass carp with an initial body weight of (20.00±0.25) g were randomly divided into 3 groups with 3 replicates per group and 40 fish per replicate. The grass carp in 3 groups were fed three kinds of isonitrogenous and isoenergetic experimental diets which supplemented with 0 (control group), 0.5% (group Ⅰ) and 1.0% (group Ⅱ) Flammulina velutipes stembase in basal diet, respectively. The experimental period was 8 weeks. The results showed as follows:1) compared with the control group, the weight gain rate of group Ⅰ and group Ⅱ was significantly increased (P<0.05), the relative intestine weight of group Ⅰ was significantly decreased (P<0.05), and the hepatopancreas index of group Ⅱ was significantly increased (P<0.05). There were no significant differences in the contents of crude fat, crude ash, crude protein and moisture among all groups (P>0.05). 2) Compared with the control group, the intestinal lipase activity of group Ⅱ was significantly increased (P<0.05), and the intestinal amylase activity of group Ⅰ and group Ⅱ was significantly decreased (P<0.05). 3) Compared with the control group, the midgut muscular layer thickness and goblet cell number of group Ⅰ and group Ⅱ were significantly increased (P<0.05). 4) Compared with the control group, the muscle hardness, gumminess and chewiness of group Ⅰ was significantly increased (P<0.05). 5) Compared with the control group, the serum glucose content and glutamic oxaloacetic transaminase activity of group Ⅰ and group Ⅱ were significantly increased (P<0.05), the serum urea nitrogen, triglyceride contents and alanine aminotransferase activity of group Ⅰ and group Ⅱ were significantly decreased (P<0.05), and the serum total cholesterol content of group Ⅱ was significantly decreased (P<0.05). In conclusion, the dietary appropriate addition of Flammulina velutipes stembase can promote the growth, improve the intestinal tissue structure, increase the intestinal digestive enzyme activities, and improve the muscle quality of grass carp.

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