动物营养学报 >

2021 , Vol. 33 >Issue 8: 4580 - 4591

DOI: https://doi.org/10.3969/j.issn.1006-267x.2021.08.038

水产动物营养与饲料 AQUATIC ANIMAL NUTRITION AND FEED

柠檬酸对草鱼生长性能、营养物质利用、血清生化指标和肠道组织形态的影响

  • 程卓 ,
  • 张国奇 ,
  • 周陆 ,
  • 徐禛 ,
  • 杨航 ,
  • 李小勤 ,
  • 冷向军
展开
  • 1. 上海海洋大学水产与生命学院, 水产科学国家级实验教学示范中心, 上海 201306;
    2. 上海海洋大学水产与生命学院, 农业农村部鱼类营养与环境生态研究中心, 上海 201306;
    3. 上海海洋大学水产与生命学院, 水产动物遗传育种中心上海市协同创新中心, 上海 201306;
    4. 上海市松江区水产良种场, 上海 201203
程卓(1995-),男,湖北京山人,硕士研究生,研究方向为水产动物营养。E-mail:1445371753@qq.com

收稿日期: 2021-01-31

  网络出版日期: 2021-08-11

基金资助

上海市科技兴农重点攻关项目[沪农科创字（2018）第2-11号]

收起

Effects of Citric Acid on Growth Performance, Nutrient Utilization, Serum Biochemical Indices and Intestinal Tissue Morphology of Grass Carp (Ctenopharyngodon idella)

  • CHENG Zhuo ,
  • ZHANG Guoqi ,
  • ZHOU Lu ,
  • XU Zhen ,
  • YANG Hang ,
  • LI Xiaoqin ,
  • LENG Xiangjun
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  • 1. National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China;
    2. Centre for Research on Environmental Ecology and Fish Nutrition of the Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai 201306, China;
    3. Shanghai Collaborative Innovation for Aquatic Animal Genetics and Breeding, Shanghai Ocean University, Shanghai 201306, China;
    4. Shanghai Songjiang Aquatic Products Breeding Farm, Shanghai 201203, China

Received date: 2021-01-31

  Online published: 2021-08-11

Supported by

 

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摘要

本试验旨在研究柠檬酸对草鱼生长性能、营养物质利用、血清生化指标和肠道组织形态的影响。在基础饲料中分别添加0（对照组）、0.15%、0.30%和0.45%的柠檬酸，配制成4种等氮等脂饲料，饲养初始平均体重为（17.0±0.2） g的草鱼60 d。结果表明：0.30%柠檬酸组具有最高的增重率（432.8%）和最低的饲料系数（1.30），其增重率较对照组提高了11.37%（P<0.05），饲料系数较对照组降低了10.34%（P<0.05）。与对照组相比，饲料中添加0.15%~0.45%的柠檬酸显著提高了磷表观消化率与沉积率以及脂肪沉积率（P<0.05），饲料中添加0.30%的柠檬酸还显著提高了蛋白质表观消化率与沉积率以及干物质表观消化率（P<0.05）。0.15%和0.30%柠檬酸组的血清超氧化物歧化酶活性较对照组显著升高（P<0.05），而丙二醛含量则较对照组显著降低（P<0.05）。0.30%和0.45%柠檬酸组的肠道蛋白酶和淀粉酶活性显著高于对照组（P<0.05）。0.15%~0.45%柠檬酸组的肠道绒毛高度和肌层厚度显著高于对照组（P<0.05），绒毛宽度各组之间无显著差异（P>0.05）。综上可知，饲料中添加柠檬酸能提高草鱼的生长性能和饲料利用率，增强血清抗氧化能力，改善肠道组织形态；草鱼饲料中柠檬酸的适宜添加量为0.30%。

关键词： 草鱼; 柠檬酸; 生长; 消化率; 沉积率; 血清生化指标; 肠道组织学

本文引用格式

程卓 , 张国奇 , 周陆 , 徐禛 , 杨航 , 李小勤 , 冷向军 . 柠檬酸对草鱼生长性能、营养物质利用、血清生化指标和肠道组织形态的影响[J]. 动物营养学报, 2021 , 33(8) : 4580 -4591 . DOI: 10.3969/j.issn.1006-267x.2021.08.038

Abstract

The study investigated the effects of citric acid on growth performance, nutrient utilization, serum biochemical indices and intestinal tissue morphology of grass carp (Ctenopharyngodon idella). Citric acid was supplemented into a basal diet at an inclusion of 0 (control group), 0.15%, 0.30% and 0.45%, and then the four isonitrogenous and isolipidemic diets were fed to grass carp[initial body weight (17.0±0.2) g] for 60 days. The results showed that 0.30% citric acid group had the highest weight gain rate (432.8%) and the lowest feed conversion ratio (1.30), which was 11.37% higher and 10.34% lower than the control group (P<0.05), respectively. The supplementation of 0.15% to 0.45% citric acid significantly increased the phosphorus apparent digestibility, phosphorus retention rate and lipid retention rate (P<0.05), and the supplementation of 0.30% citric acid group significantly increased the protein retention rate, dry matter apparent digestibility and protein apparent digestibility compared with the control group (P<0.05). Compared with the control group, the serum superoxide dismutase (SOD) activity in 0.15% and 0.30% citric acid groups was significantly increased, while the serum malondialdehyde (MDA) content was significantly decreased (P<0.05). The 0.30% and 0.45% citric acid groups showed higher intestinal protease and amylase activities compared with the control group (P<0.05). The intestinal villus height and muscle layer thickness in 0.15% to 0.45% citric acid groups were significantly higher than those in the control group (P<0.05), but there was no significant difference in villus width among all groups (P>0.05). In conclusion, dietary citric acid can promote the growth performance and feed utilization, enhance the serum antioxidant capacity, and improve the intestinal tissue morphology of grass carp, and the optimal supplemental level of citric acid in the diet of grass carp is 0.30%.

Key words： grass carp; citric acid; growth; digestibility; retention rate; serum biochemical indices; intestinal histology

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