分子与细胞营养 MOLECULAR AND CELLULAR NUTRITION

N-氨甲酰谷氨酸、脯氨酸及腐胺对仔猪空肠和回肠Na+依赖性中性氨基酸转运载体2表达及营养感应信号的调控作用

  • 王婧 ,
  • 李光燃 ,
  • 肖玉欣 ,
  • 邓远坤 ,
  • 唐宇龙 ,
  • 谭碧娥
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  • 湖南农业大学动物科学技术学院, 长沙 410128
王婧(1990-),女,内蒙古赤峰人,博士,主要从事单胃动物营养研究。E-mail:jingwang023@gmail.com

收稿日期: 2021-01-23

  网络出版日期: 2021-08-11

基金资助

博士后创新人才支持计划(BX20180096);湖南创新型省份建设专项(2019RS30219)

Regulation of N-Carbamyl Glutamate, Proline and Putrescine on Sodium Dependent Neutral Amino Acid Transporter-2 Expression and Nutrition Sensing Signaling in Jejunum and Ileum of Piglets

  • WANG Jing ,
  • LI Guangran ,
  • XIAO Yuxin ,
  • DENG Yuankun ,
  • TANG Yulong ,
  • TAN Bi'e
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  • College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China

Received date: 2021-01-23

  Online published: 2021-08-11

Supported by

 

摘要

本研究旨在探讨Na+依赖性中性氨基酸转运载体2(SNAT2)介导多胺及其前体物质对仔猪肠黏膜营养感应信号的调控作用。试验选取32头哺乳仔猪,随机分为4组,每组8头猪,自出生当日开始,每日2次分别灌服生理盐水(对照组)、腐胺(5 mg/kg BW)、脯氨酸(25 mg/kg BW)和N-氨甲酰谷氨酸(NCG,8 mg/kg BW)。14日龄断奶,断奶第3天采血后屠宰,采集空肠和回肠样品。结果表明:与对照组相比,灌服腐胺、脯氨酸和NCG显著提高仔猪平均日增重(P<0.05);灌服腐胺和NCG显著提高空肠和回肠黏膜SNAT2的蛋白表达水平(P<0.05);灌服脯氨酸和NCG显著提高了空肠黏膜氨基酸转运感应相关基因[质子耦合氨基酸转运蛋白1(PAT1)、钙感应受体(CasR)、磷脂酶Cβ2(PLCβ2)和味觉受体1家族成员3(T1R3)]的mRNA相对表达量(P<0.05),NCG还显著上调哺乳动物雷帕霉素靶蛋白(mTOR)信号通路相关基因[促分裂原活化蛋白激酶3(MAP4K3)和mTOR]的mRNA相对表达量(P<0.05);灌服NCG显著提高了血清中胆囊收缩素(CCK)含量(P<0.05)及空肠黏膜CCK受体mRNA相对表达量(P<0.05)。综上所述,NCG可通过上调仔猪肠道氨基酸转运载体相关基因的表达,调节氨基酸感应途径信号分子的释放和基因表达。

本文引用格式

王婧 , 李光燃 , 肖玉欣 , 邓远坤 , 唐宇龙 , 谭碧娥 . N-氨甲酰谷氨酸、脯氨酸及腐胺对仔猪空肠和回肠Na+依赖性中性氨基酸转运载体2表达及营养感应信号的调控作用[J]. 动物营养学报, 2021 , 33(8) : 4615 -4625 . DOI: 10.3969/j.issn.1006-267x.2021.08.041

Abstract

The purpose of this study was to investigate regulation of the sodium dependent neutral amino acid transporter-2 (SNAT2) on the nutrition sensing signaling of intestinal mucosa of piglets via polyamines. Thirty-two neonatal piglets (Duroc×Landrace×Large Yorkshire) from 4 litters (8 piglets per litter) were assigned to 4 groups, which received oral administration of equal volumes of saline (control group), putrescine (5 mg/kg BW), proline (25 mg/kg BW) and N-carbamylglutamate (NCG, 8 mg/kg BW) twice daily from days 1 to 14. At day 3 post-weaning, all piglets were slaughtered and their blood, jejunum and ileum were collected. Results showed that compared with control group, oral administration with putrescine, proline and NCG significantly promoted the average daily gain of piglets (P<0.05); supplementation with putrescine and NCG significantly increased the SNAT2 protein expression level in the jejunal and ileal mucosa (P<0.05); proline and NCG treatments significantly increased the mRNA relative expression level of amino acid transport signaling-related genes[proton coupled amino acid transporter 1 (PAT1), calcium sensing receptor (CasR), phospholipase β2 (PLCβ2) and taste receptor 1 family member 3 (T1R3)] in the jejunal mucosa of piglets (P<0.05); meanwhile, NCG treatment significantly increased the mRNA expression level of mammalian target of rapamycin (mTOR) signaling pathway-related genes[mitogen-activated protein kinase 3 (MAP4K3) and mTOR] (P<0.05), as well as the content of cholecystokinin (CCK) in serum (P<0.05) and the mRNA relative expression level of the receptor of CCK in the jejunal mucosa (P<0.05). Therefore, NCG can regulate the release of signal molecules and gene expression of amino acid sensing pathway by up-regulating the expression of genes related to amino acid transporters in intestinal tract of piglets.

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