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耐镉乳酸菌对镉暴露仔鸡体重、脏器系数和组织中矿物元素含量的影响

  • 蒲俊华 ,
  • 陈大伟 ,
  • 刘茵茵 ,
  • 李惠嘉 ,
  • 陆俊贤 ,
  • 张小燕 ,
  • 唐梦君 ,
  • 唐修君 ,
  • 高玉时
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  • 1. 江苏省家禽科学研究所, 扬州 225125;
    2. 山东农业大学动物科技学院/动物医学院, 泰安 271018
蒲俊华(1975-),女,四川蒲江人,副研究员,博士,研究方向为家禽营养与禽产品质量安全。E-mail:apu123@sina.com

收稿日期: 2021-01-21

  网络出版日期: 2021-08-11

基金资助

“十三五”国家重点研发项目(2016YFD0501208);江苏现代农业(肉鸡)产业技术体系建设项目(JATS[2020]359);江苏省家禽遗传育种重点实验室项目(JQLAB-ZZ-201707);扬州市现代农业项目(YZ2020049);扬州市社会发展项目(YZ2020061)

Effects of Cadmium-Tolerant Lactobacillus on Body Weight, Organ Coefficients and Contents of Mineral Elements in Tissues of Cadmium-Exposed Chickens

  • PU Junhua ,
  • CHEN Dawei ,
  • LIU Yinyin ,
  • LI Huijia ,
  • LU Junxian ,
  • ZHANG Xiaoyan ,
  • TANG Mengjun ,
  • TANG Xiujun ,
  • GAO Yushi
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  • 1. Jiangsu Institute of Poultry Sciences, Yangzhou 225125, China;
    2. College of Animal Science and Veterinary Medicine, Shandong Agriculture University, Tai'an 271018, China

Received date: 2021-01-21

  Online published: 2021-08-11

Supported by

 

摘要

本试验旨在探究耐镉乳酸菌对镉暴露仔鸡体重、脏器系数和组织中镉、钙、铁、锌、铜、锰、硒含量影响。选取1日龄如皋黄鸡30只,随机分为3组,每组10个重复,每个重复1只鸡。对照组试验鸡第3~6周灌喂1 mL/(d·只)无菌生理盐水;镉暴露组试验鸡第3~6周灌喂1 mL/(d·只)无菌生理盐水,并在第4~6周饮水中添加5 mg/L镉;镉+耐镉乳酸菌组试验鸡第3~6周灌喂1 mL/(d·只)耐镉乳酸菌液(1×109 CFU/mL),并在第4~6周饮水中添加5 mg/L镉。预试期2周,正试期4周。结果表明:1)各组之间仔鸡体重无显著差异(P>0.05)。2)镉暴露组和镉+耐镉乳酸菌组心脏系数显著低于对照组(P<0.05),且镉暴露组和镉+耐镉乳酸菌组之间心脏系数无显著差异(P>0.05)。3)镉暴露组的各组织中镉含量显均显著高于对照组(P<0.05),且各组织中镉含量的高低依次为肾脏>肝脏>脾脏>肺脏>心脏>胸肌。镉+耐镉乳酸菌组的脾脏中镉含量显著低于镉暴露组(P<0.05)。4)镉+耐镉乳酸菌组肝脏中硒含量显著低于对照组和镉暴露组(P<0.05)。镉+耐镉乳酸菌组和镉暴露组肾脏中硒含量显著低于对照组(P<0.05)。镉暴露组肺脏中钙含量显著高于对照组和镉+耐镉乳酸菌组(P<0.05),镉+耐镉乳酸菌组和镉暴露组肺脏中锌含量显著低于对照组(P<0.05),镉+耐镉乳酸菌组肺脏中铜含量显著低于对照组(P<0.05),镉暴露组肺脏中锰含量显著高于对照组和镉+耐镉乳酸菌组(P<0.05)。镉+耐镉乳酸菌组脾脏中钙和铜含量显著低于对照组和镉暴露组(P<0.05)。镉暴露组心脏中钙含量显著低于对照组(P<0.05),镉+耐镉乳酸菌组和镉暴露组心脏中硒含量显著高于对照组(P<0.05)。由此可见,仔鸡镉暴露时,心脏系数降低,肝脏、肾脏、肺脏、脾脏、心脏和胸肌中镉含量增加,肾脏中硒含量和心脏中钙含量降低;添加耐镉乳酸菌对镉暴露仔鸡心脏系数的降低无改善作用,但降低了脾脏和肝脏中镉含量,并增加了心脏中硒和钙含量。

本文引用格式

蒲俊华 , 陈大伟 , 刘茵茵 , 李惠嘉 , 陆俊贤 , 张小燕 , 唐梦君 , 唐修君 , 高玉时 . 耐镉乳酸菌对镉暴露仔鸡体重、脏器系数和组织中矿物元素含量的影响[J]. 动物营养学报, 2021 , 33(8) : 4730 -4739 . DOI: 10.3969/j.issn.1006-267x.2021.08.051

Abstract

The aim of this experiment was to study the effects of cadmium (Cd)-tolerant Lactobacillus on body weight, organ coefficients and contents of Cd, calcium (Ca), iron (Fe), zinc (Zn), copper (Cu), manganese (Mn) and selenium (Se) in tissues of Cd-exposed chickens. A total of 30 one-day-old Rugao yellow chickens were randomly divided into 3 groups with 10 replicates per group and 1 chicken per replicate. Chickens in the control group were fed 1 mL/(d·bird) of sterile physiological saline via gavage from the 3rd week to the 6th week; chickens in the Cd-exposed group were fed 1 mL/(d·bird) of sterile physiological saline via gavage from the 3rd week to the 6th week, and were given drinking water with 5 mg/L Cd from the 4th week to the 6th week; chickens in the Cd+Cd-tolerant Lactobacillus group were fed 1 mL/(d bird) of Cd-tolerant Lactobacillus liquid (1×109 CFU/mL) via gavage from the 3rd week to the 6th week, and were given drinking water with 5 mg/L Cd from the 4th week to the 6th week. The experimental period was 6 weeks, including 2 weeks of preliminary trial period and 4 weeks of trial period. The results showed as follows:1) there was no significant difference in body weight of chickens among all groups (P>0.05). 2) The heart coefficient of the Cd-exposed group and the Cd+Cd-tolerant Lactobacillus group was significantly lower than that of the control group (P<0.05), and there was no significant difference in the heart coefficient between the Cd-exposed group and the Cd+Cd-tolerant Lactobacillus group (P>0.05). 3) The contents of Cd in all tissues of the Cd-exposed group were significantly higher than those of the control group (P<0.05), and the contents of Cd in tissues from high to low were kidney>liver>spleen>lung>heart>breast muscle. The spleen Cd content of the Cd+Cd-tolerant Lactobacillus group was significantly lower than that of the Cd-exposed group (P<0.05). 4) The liver Se content of the Cd+Cd-tolerant Lactobacillus group was significantly lower than that of the control group and the Cd-exposed group (P<0.05). The kidney Se content of the Cd+Cd-tolerant Lactobacillus group and the Cd-exposed group was significantly lower than that of the control group (P<0.05). The lung Ca content of the Cd-exposed group was significantly higher than that of the control group and the Cd+Cd-tolerant Lactobacillus group (P<0.05), the lung Zn content of the Cd+Cd-tolerant Lactobacillus group and Cd-exposed group was significantly lower than that of the control group (P<0.05). The lung Cu content of the Cd+Cd-tolerant Lactobacillus group was significantly lower than that of the control group (P<0.05), and the lung Mn content of the Cd-exposed group was significantly higher than that of the control group and the Cd+Cd-tolerant Lactobacillus group (P<0.05). The contents of Ca and Cu in spleen of the Cd+Cd-tolerant Lactobacillus group were significantly lower than those of the control group and Cd-exposed group (P<0.05). The heart Ca content of the Cd-exposed group was significantly lower than that of the control group (P<0.05), and the heart Se content of the Cd+Cd-tolerant Lactobacillus group and Cd-exposed group was significantly higher than that of the control group (P<0.05). Thus, when the chickens are exposed to Cd, the heart coefficient decreases, the contents of Cd in liver, kidney, lung, spleen, heart and breast muscle increase, and the kidney Se content and heart Ca content decrease. The supplementation of Cd-tolerant Lactobacillus has no significant effect on ameliorating heart coefficient, but it reduces the spleen and liver Cd content, and increases the contents of Se and Ca in heart.

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