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低锌饲粮添加载锌蒙脱石对肉鸡免疫功能和肝脏含锌酶活性的影响

  • 王海波 ,
  • 秦士贞 ,
  • 李金录 ,
  • 武真邑 ,
  • 龚莉媛 ,
  • 裴文刚 ,
  • 车育彦 ,
  • 杨敏敏 ,
  • 史兆国
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  • 甘肃农业大学动物科学技术学院, 兰州 730070
王海波(1992-),男,甘肃会宁人,硕士研究生,从事家禽营养研究。E-mail:wanghaibo8815@163.com

收稿日期: 2021-01-20

  网络出版日期: 2021-08-11

基金资助

国家自然科学基金(03119023);甘肃农业大学人才专项(2017RCZX-18);甘肃省青年科技基金计划(20JR5RA013);甘肃省现代农业产业技术体系猪鸡产业岗位(GARS-ZJ-2)

Effects of Adding Zinc-Montmorillonite in Low Zinc Diet on Immune Function and Zinc-Containing Enzyme Activities in Liver of Broilers

  • WANG Haibo ,
  • QIN Shizhen ,
  • LI Jinlu ,
  • WU Zhenyi ,
  • GONG Liyuan ,
  • PEI Wengang ,
  • CHE Yuyan ,
  • YANG Minmin ,
  • SHI Zhaoguo
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  • College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China

Received date: 2021-01-20

  Online published: 2021-08-11

Supported by

 

摘要

本试验旨在研究低锌饲粮添加载锌蒙脱石(Zn-MMT)对肉鸡免疫功能、肝脏含锌酶活性的影响。随机选择1日龄健康科宝公雏240只,按体重随机分成5组,每组6个重复,每个重复8只仔鸡。对照组(CK组)饲喂玉米-豆粕型基础饲粮,试验组分别在基础饲粮中替代载体添加20、40、60和80 mg/kg Zn-MMT (均以锌含量计算),自由采食饮水,试验期为42 d,测定空肠黏膜免疫球蛋白含量、肝脏含锌酶活性、血清细胞因子含量、脾脏免疫相关基因表达。结果表明:1)相比CK组,添加40和60 mg/kg的Zn-MMT显著提高肉鸡血清中干扰素-γ(IFN-γ)的含量(P<0.05),对血清白细胞介素-1β(IL-1β)、白细胞介素-2(IL-2)、白细胞介素-4(IL-4)的含量有升高趋势(0.05<P<0.10),但对血清中免疫球蛋白A (IgA)、免疫球蛋白M (IgM)、肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)的含量无显著影响(P>0.05)。2)相比CK组,添加Zn-MMT对肉鸡21和42日龄空肠黏膜的免疫球蛋白G (IgG)、分泌型免疫球蛋白(sIgA)含量均无显著影响(P>0.05)。3)相比CK组,添加Zn-MMT显著提高肉鸡21和42日龄肉鸡肝脏中铜锌超氧化物歧化酶(Cu-Zn SOD)活性,但对碱性磷酸酶(ALP)、乳酸脱氢酶(LDH)、苹果酸脱氢酶(MDH)活性无显著影响(P>0.05)。4)相比CK组,添加40 mg/kg的Zn-MMT显著提高肉鸡脾脏核转录因子-κBp65(NF-κBp65)、肿瘤坏死因子受体相关因子-2(TRAF-2)、肿瘤坏死因子受体超家族1B (TNFRSF1B)、核因子κB抑制蛋白-α(IκB-α)、IL-1βIL-4、IL-6、白细胞介素-10(IL-10)基因相对表达量(P<0.05),但对转化生长因子-β4(TFG-β4)、IFN-γ基因相对表达量无显著影响(P>0.05)。综上所述,低锌饲粮添加Zn-MMT可显著提高脾脏免疫相关基因的表达,增强机体免疫力,增强含锌酶活性。

本文引用格式

王海波 , 秦士贞 , 李金录 , 武真邑 , 龚莉媛 , 裴文刚 , 车育彦 , 杨敏敏 , 史兆国 . 低锌饲粮添加载锌蒙脱石对肉鸡免疫功能和肝脏含锌酶活性的影响[J]. 动物营养学报, 2021 , 33(8) : 4779 -4790 . DOI: 10.3969/j.issn.1006-267x.2021.08.056

Abstract

This experiment was conducted to investigate the effects of zinc-montmorillonite (Zn-MMT)on immune function and zinc-containing enzyme activities of broilers fed a low zinc diet. A total of 240 one-day-old male Cob broiler were randomly assigned to 5 groups with 6 replicates per group and 8 birds per replicate. The birds were fed a corn-soybean meal basal diet (CK group) or one of the four Zn-MMT diets, which were supplemented with 20, 40, 60,80 mg/kg Zn-MMT(Zinc content measurement) based on the basal diet. The broilers were provided the diets in ad libitum basis and the experiment lasted for 42 days. The activities of immunoglobulin in jejunal mucosa and Zn-related enzymes in liver were measured. Meanwhile, serum cytokine contents and the expression levels of genes related to spleen immunity was measured. The results showed as follows:1) compared with CK group, adding 40 and 60 mg/kg Zn-MMT significantly increased the content of interferon-γ in broiler serum (P<0.05), and the contents of interleukin-1, interleukin-2 and interleukin-4 showed an increasing trend (0.05<P<0.10), but had no significant influence on the contents of immunoglobulin A, immunoglobulin M, tumor necrosis factor-α and interleukin-6 in broiler serum (P>0.05). 2) Compared with CK group,adding Zn-MMT had no significant effect on immunoglobulin G and secretory immunoglobulin contents of jejunal mucosa of broilers aged 21 and 42 days (P>0.05). 3) Compared with CK group,adding Zn-MMT significantly increased the activity of Cu-Zn superoxide dismutase (P<0.05),but had no significant effect on the activities of alkaline phosphatase, lactate dehydrogenase and malate dehydrogenase in the liver (P>0.05). 4) Compared with CK group,adding 40 mg/kg Zn-MMT significantly increased the expression level of nuclear transcription factor-κBp65, tumor necrosis factor receptor-related factors 2, tumor necrosis factor receptor superfamily 1B,nuclear factor κB inhibits protein α,interleukin-1β,interleukin-4,interleukin-6,interleukin-10 genes in the spleen at 42 days of age (P<0.05), but had no significant effect on the expression levels of transformed growth factor-β4 and interferon-γ genes (P>0.05). It is concluded that dietary supplementation with Zn-MMT can significantly improve the expression of spleen immune-related genes, enhance the immune function, and enhance the activity of zinc-containing enzymes in the low zinc diet.

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