动物营养学报 >

2021 , Vol. 33 >Issue 10: 5569 - 5580

DOI: https://doi.org/10.3969/j.issn.1006-267x.2021.10.017

禽营养与饲料 POULTRY NUTRITION AND FEED

叶黄素对脂多糖应激黄羽肉鸡生长性能、空肠形态和盲肠微生物的影响

  • 王美艳 ,
  • 童玉鑫 ,
  • 闵遥 ,
  • 王长康 ,
  • 高玉云
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  • 福建农林大学动物科学学院/蜂学学院, 福州 350002
王美艳(1995-),女,福建顺昌人,硕士研究生,从事动物营养与饲料的研究。E-mail:505105793@qq.com

收稿日期: 2021-03-11

  网络出版日期: 2021-10-16

基金资助

国家自然科学基金项目（31802079）；福建省家禽产业体系岗位专家（K83139297，2019-2022）

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Effects of Lutein on Growth Performance, Jejunum Morphology and Cecal Microorganisms of Lipopolysaccharide-Stimulated Yellow-Feathered Broilers

  • WANG Meiyan ,
  • TONG Yuxin ,
  • MIN Yao ,
  • WANG Changkang ,
  • GAO Yuyun
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  • College of Animal Science/College of Bee Science, Fujian Agricultural and Forestry University, Fuzhou 350002, China

Received date: 2021-03-11

  Online published: 2021-10-16

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摘要

本试验旨在研究不同水平叶黄素对脂多糖（LPS）应激黄羽肉鸡生长性能、空肠形态和盲肠微生物的影响。选择体重相近的1日龄健康黄羽肉公鸡288只，随机分为3个组，每组8个重复，每个重复12只鸡。对照组饲喂基础饲粮，试验组分别饲喂在基础饲粮中添加20和40 mg/kg叶黄素的试验饲粮。于肉鸡21、23和25日龄时连续3次腹腔注射1 mg/kg LPS，试验期26 d。结果表明：1）与对照组相比，饲粮中添加40 mg/kg叶黄素可显著提高LPS应激黄羽肉鸡22~26日龄和1~26日龄的平均日增重（P<0.05），显著降低22~26日龄的料重比（P<0.05）。2）与对照组相比，饲粮中添加20和40 mg/kg叶黄素可显著提高LPS应激黄羽肉鸡的空肠绒毛高度（P<0.05），添加40 mg/kg叶黄素可显著提高空肠绒毛高度/隐窝深度（V/C）（P<0.05）。3）与对照组相比，饲粮中添加20和40 mg/kg叶黄素可显著提高LPS应激黄羽肉鸡盲肠微生物Shannon指数（P<0.05），且菌群结构较为相似，但与对照组样本距离较远；显著提高厚壁菌门（Firmicutes）和艾森伯格菌属（Eisenbergiella）相对丰度（P<0.05），显著降低变形菌门（Proteobacteria）和未鉴定的肠杆菌科（unidentified Enterobacteriaceae）相对丰度（P<0.05）；与氨基酸代谢、碳水化合物代谢和遗传信息相关的京都基因与基因组百科全书（KEGG）通路在20和40 mg/kg叶黄素组中显著富集（P<0.05）。综上所述，饲粮中添加20和40 mg/kg叶黄素能改善LPS应激下黄羽肉鸡空肠形态和盲肠微生物菌群结构组成，其中40 mg/kg叶黄素组促生长效果最佳。

关键词： 叶黄素; 脂多糖; 肉鸡; 生长性能; 空肠形态; 盲肠微生物

本文引用格式

王美艳 , 童玉鑫 , 闵遥 , 王长康 , 高玉云 . 叶黄素对脂多糖应激黄羽肉鸡生长性能、空肠形态和盲肠微生物的影响[J]. 动物营养学报, 2021 , 33(10) : 5569 -5580 . DOI: 10.3969/j.issn.1006-267x.2021.10.017

Abstract

This experiment aimed to study the effects of different levels of lutein on growth performance, intestinal morphology and cecal microorganisms of lipopolysaccharide (LPS)-stimulated yellow-feather broilers. Two hundred eighty-eight one-day-old healthy yellow-feathered male broilers with similar body weight were randomly divided into 3 groups with 8 replicates per group and 12 broilers per replicate. Broilers in control group were fed a basal diet, and the others in experimental groups were fed the basal diets supplemented with 20 and 40 mg/kg lutein. Three consecutive intraperitoneal injections of 1 mg/kg LPS were given to broilers at 21, 23 and 25 days of age. The experimental period lasted for 26 days. The results showed as follows:1) compared with control group, dietary 40 mg/kg lutein significantly increased average daily gain of LPS-stimulated yellow-feathered broilers from 21 to 26 days of age and 1 to 26 days of age (P<0.05), and significantly decreased the ratio of feed to gain of broilers from 22 to 26 days of age (P<0.05). 2) Compared with control group, dietary 20 and 40 mg/kg lutein significantly increased villus height of jejunum of LPS-stimulated yellow-feathered broilers (P<0.05), and dietary 40 mg/kg lutein significantly increased the ratio of villus height to crypt depth (V/C) of jejunum (P<0.05). 3) Compared with control group, dietary 20 and 40 mg/kg lutein significantly increased the Shannon index of cecal microorganism of LPS-stimulated yellow-feathered broilers (P<0.05); and the microflora structure was similar, but the distance to control group was far. Compared with control group, dietary 20 and 40 mg/kg lutein significantly increased relative abundance of Firmicutes and Eisenbergiella (P<0.05), and significantly decreased relative abundance of Proteobacteria and unidentified Enterobacteriaceae (P<0.05); and Kyoto Encyclopedia of genes and genomes (KEGG) pathways related to amino acid, carbohydrate metabolism and genetic information processing were significantly enriched in 20 and 40 mg/kg lutein groups (P<0.05). In conclusion, dietary 20 and 40 mg/kg lutein can improve jejunal morphology and cecal microflora composition of LPS-stimulated yellow-feathered broilers, and 40 mg/kg lutein has the best growth promoting effect.

Key words： lutein; LPS; broilers; growth performance; jejunum morphology; cecal microorganisms

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