禽营养与饲料 POULTRY NUTRITION AND FEED

姜黄素对中速型黄羽肉鸡生长性能、腹脂沉积、抗氧化能力、肝脏脂肪代谢相关酶活性及基因表达的影响

  • 樊祥宇 ,
  • 张富群 ,
  • 黄泰来 ,
  • 曹满湖
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  • 湖南农业大学动物科学技术学院, 长沙 410128
樊祥宇(1995-),男,湖南永州人,硕士研究生,研究方向为动物营养与饲料科学。E-mail:3030354070@qq.com

收稿日期: 2021-03-12

  网络出版日期: 2021-10-16

基金资助

长沙市生鲜肉品安全现状调查与对策研究(kq1801018)

Effects of Curcumin on Growth Performance, Abdominal Fat Deposition, Antioxidant Capacity, Liver Enzyme Activities and Gene Expression Related to Lipid Metabolism of Medium Speed Yellow-Feathered Broilers

  • FAN Xiangyu ,
  • ZHANG Fuqun ,
  • HUANG Tailai ,
  • CAO Manhu
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  • College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China

Received date: 2021-03-12

  Online published: 2021-10-16

Supported by

 

摘要

本试验旨在研究姜黄素对中速型黄羽肉鸡生长性能、腹脂沉积、抗氧化能力和肝脏脂肪代谢相关酶活性及基因表达的影响。试验选用28日龄中速型黄羽肉鸡384只,随机分为4个组,每组6个重复,每个重复16羽鸡。对照组饲喂基础饲粮,试验组分别饲喂在基础饲粮中添加50、100和200 mg/kg姜黄素的试验饲粮。试验期42 d,分为29~42日龄、43~56日龄和57~70日龄3个阶段。结果表明:与对照组相比,1)饲粮中添加100和200 mg/kg姜黄素显著降低57~70日龄和29~70日龄中速型黄羽肉鸡的平均日采食量(P<0.05)。2)饲粮中添加50、100和200 mg/kg姜黄素显著降低中速型黄羽肉鸡70日龄时的腹脂率(P<0.05),其中以200 mg/kg姜黄素添加组的效果最佳。3)43~56日龄时,饲粮中添加200 mg/kg姜黄素极显著降低中速型黄羽肉鸡的血清丙二醛(MDA)含量和超氧化物歧化酶(SOD)活性(P<0.01);57~70日龄时,饲粮中添加200 mg/kg姜黄素极显著降低血清MDA含量(P<0.01),显著提高血清谷胱甘肽过氧化物酶(GSH-Px)活性和总抗氧化能力(T-AOC)(P<0.05)。4)饲粮中添加50和200 mg/kg姜黄素显著降低中速型黄羽肉鸡的肝脏脂肪酸合成酶(FAS)活性(P<0.05);饲粮中添加50、100和200 mg/kg姜黄素均显著提高肝脏激素敏感性脂肪酶(HSL)活性(P<0.05)。5)饲粮中添加200 mg/kg姜黄素显著降低中速型黄羽肉鸡肝脏乙酰辅酶A羧化酶(ACC)的mRNA相对表达量(P<0.05)。综上所述,姜黄素能提高中速型黄羽肉鸡的抗氧化能力,调节肝脏脂肪代谢,降低腹脂沉积,且生长后期(43~70日龄)的效果优于生长前期(29~42日龄),其作用原理与姜黄素降低肝脏FASACC的mRNA相对表达量有关。

本文引用格式

樊祥宇 , 张富群 , 黄泰来 , 曹满湖 . 姜黄素对中速型黄羽肉鸡生长性能、腹脂沉积、抗氧化能力、肝脏脂肪代谢相关酶活性及基因表达的影响[J]. 动物营养学报, 2021 , 33(10) : 5581 -5590 . DOI: 10.3969/j.issn.1006-267x.2021.10.018

Abstract

The purpose of this study was to explore the effects of curcumin on growth performance, abdominal fat deposition, antioxidant capacity and liver enzyme activities and gene expression related to lipid metabolism of medium speed yellow-feathered broilers. Three hundred and eighty-four 28-day-old medium speed yellow-feathered broilers were randomly divided into 4 groups with 6 replicates per group and 16 broilers per replicate. Broilers in control group were fed a basal diet, and the others in experimental groups were fed the basal diets supplemented with 50, 100 and 200 mg/kg curcumin, respectively. The experimental period lasted for 42 days including 3 stages of 29 to 42 days of age, 43 to 56 days of age and 57 to 70 days of age. The results showed as follows:compared with control group, 1) dietary 100 and 200 mg/kg curcumin significantly decreased average daily feed intake of medium speed yellow-feathered broilers during 57 to 70 days of age and 29 to 70 days of age (P<0.05). 2) Dietary 50, 100 and 200 mg/kg curcumin significantly decreased abdominal fat rate of medium speed yellow-feathered broilers at 70 days of age (P<0.05), and 200 mg/kg curcumin had the best effects. 3) Dietary 200 mg/kg curcumin significantly decreased the content of malondialdehyde (MDA) and the activity of superoxide dismutase (SOD) in serum of medium speed yellow-feathered broilers during 43 to 56 days of age (P<0.01), dietary 200 mg/kg curcumin significantly decreased the content of MDA in serum (P<0.01), and significantly increased the activity of glutathione peroxidase (GSH-Px) and total antioxidant capacity (T-AOC) in serum during 57 to 70 days of age (P<0.05). 4) Dietary 50 and 200 mg/kg curcumin significantly decreased the activity of fatty acid synthase (FAS) in liver of medium speed yellow-feathered broilers (P<0.05), and dietary 50, 100 and 200 mg/kg curcumin significantly increased the activity of hormone sensitive lipase (HSL) in liver (P<0.05). 5) Dietary 200 mg/kg curcumin significantly decreased mRNA relative expression level of Acetyl CoA carboxylase (ACC) in liver of medium speed yellow-feathered broilers (P<0.05). In conclusion, curcumin can improve antioxidant capacity, regulate liver lipid metabolism and reduce abdominal fat deposition of medium speed yellow-feathered broilers, and the effects in later growth period (43 to 70 days of age) were better than those in early growth period (29 to 42 days of age). The mechanism of curcumin is related to the decrease of mRNA relative expression levels of FAS and ACC in liver.

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