动物营养学报 >

2021 , Vol. 33 >Issue 10: 5591 - 5600

DOI: https://doi.org/10.3969/j.issn.1006-267x.2021.10.019

禽营养与饲料 POULTRY NUTRITION AND FEED

柑橘提取物对快速型黄羽肉鸡运输应激后体重损失、屠宰性能、抗氧化能力和肉品质的影响

  • 李贞明 ,
  • 余苗 ,
  • 马现永 ,
  • 容庭 ,
  • 崔艺燕 ,
  • 李书宏 ,
  • 刘志昌
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  • 1. 广东省农业科学院动物科学研究所, 农业部华南动物营养与饲料重点实验室, 畜禽育种国家重点实验室, 广东省畜禽育种与营养研究重点实验室, 广东省畜禽肉品质量安全控制与评定工程技术研究中心, 广州 510640;
    2. 清远市龙发种猪有限公司, 英德 511500
李贞明(1990-),男,山东临沂人,硕士,从事生态健康养殖研究。E-mail:lzm900120@163.com

收稿日期: 2021-03-29

  网络出版日期: 2021-10-16

基金资助

“十三五”国家重点研发计划（2016YFD0501210）；广东省现代农业产业技术体系饲料创新团队项目（2020KJ115）；广东省农业科学院农业优势产业科学团队建设项目（202118TD）；科技创新战略专项资金（高水平农科院建设）——优秀博士（R2020YJ-YB2002）及金颖之光（R2017YJ-JG0001）；广州市民生科技攻关计划项目（201903010083）；清远市清城区清远鸡产业园项目（GDSCYY2018-032）；科技创新战略专项资金（高水平农科院建设）——杰出人才项目（R2020PY-JC001）

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Effects of Citrus Extract on Body Weight Loss, Slaughter Performance, Antioxidant Capacity and Meat Quality of Rapidly-Growing Yellow-Feathered Broilers after Transportation Stress

  • LI Zhenming ,
  • YU Miao ,
  • MA Xianyong ,
  • RONG Ting ,
  • CUI Yiyan ,
  • LI Shuhong ,
  • LIU Zhichang
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  • 1. Guangdong Engineering Technology Research Center of Animal Meat Quality and Safety Control and Evaluation, Guangdong Public Laboratory of Animal Breeding and Nutrition, State Key Laboratory of Livestock and Poultry Breeding, Key Laboratory of Animal Nutrition and Feed Science in South China, Ministry of Agriculture, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China;
    2. Qingyuan Longfa pig breeding Co., Ltd., Yingde 511500, China

Received date: 2021-03-29

  Online published: 2021-10-16

Supported by

 

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摘要

本试验旨在研究柑橘提取物对快速型黄羽肉鸡运输应激后体重损失、屠宰性能、抗氧化能力和肉品质的影响。选取1日龄快速型黄羽肉鸡母鸡360只，随机分成2组，每组6个重复，每个重复30只鸡。对照组饲喂玉米-豆粕型基础饲粮，柑橘提取物组饲喂在基础饲粮中添加10 mg/kg柑橘提取物的试验饲粮。试验期63 d。试验结束后，采用2×3因子试验设计，每个重复选6只鸡进行运输应激试验，分别运输0、2和4 h，运输结束后进行屠宰取样。结果表明：1）饲粮处理与运输时间对快速型黄羽肉鸡的体重损失存在显著交互作用（P<0.05）；与对照组相比，运输2和4 h后柑橘提取物组的体重损失显著降低（P<0.05）；运输时间显著影响体重损失（P<0.05），随着时间的延长，体重损失越来越大（P<0.05）。2）与对照组相比，运输0、2和4 h后饲粮添加柑橘提取物可显著提高快速型黄羽肉鸡的胸肌率和腿肌率（P<0.05）。3）与对照组相比，运输0、2和4 h后柑橘提取物组快速型黄羽肉鸡胸肌中的丙二醛（MDA）含量显著降低（P<0.05），运输2和4 h后柑橘提取物组胸肌中的谷胱甘肽过氧化物酶（GSH-Px）活性显著升高（P<0.05）；运输时间显著影响胸肌中的MDA含量和GSH-Px活性（P<0.05），随着时间的延长，MDA含量显著升高（P<0.05），GSH-Px活性显著降低（P<0.05）。4）饲粮处理和运输时间对快速型黄羽肉鸡胸肌的45 min亮度（L*45 min）和45 min红度（a*45 min）存在显著交互作用（P<0.05）；与对照组相比，运输0、2和4 h后柑橘提取物组胸肌的L*45 min显著降低（P<0.05），而a*45 min显著升高（P<0.05）；运输时间显著影响L*45 min和a*45 min（P<0.05），随着时间的延长，L*45 min显著升高（P<0.05），而a*45 min显著降低（P<0.05）。与对照组相比，运输0、2和4 h后柑橘提取物组快速型黄羽肉鸡胸肌的剪切力显著降低（P<0.05）；运输时间显著影响胸肌的剪切力（P<0.05），随着时间的延长，剪切力显著升高（P<0.05）。由此可见，运输应激显著影响黄羽肉鸡的体重损失和肉品质，而饲粮中添加柑橘提取物可降低运输后黄羽肉鸡的体重损失，提高胸肌抗氧化能力，改善肉品质，缓解运输应激对黄羽肉鸡的影响。

关键词： 肉鸡; 柑橘提取物; 运输应激; 体重损失; 屠宰性能; 抗氧化能力; 肉品质

本文引用格式

李贞明 , 余苗 , 马现永 , 容庭 , 崔艺燕 , 李书宏 , 刘志昌 . 柑橘提取物对快速型黄羽肉鸡运输应激后体重损失、屠宰性能、抗氧化能力和肉品质的影响[J]. 动物营养学报, 2021 , 33(10) : 5591 -5600 . DOI: 10.3969/j.issn.1006-267x.2021.10.019

Abstract

This experiment was conducted to study the effects of citrus extract on body weight loss, slaughter performance, antioxidant capacity and meat quality of rapidly-growing yellow-feathered broilers after transportation stress. Three hundred and sixty one-day-old rapidly-growing yellow-feathered female broilers were randomly divided into 2 groups with 6 replicates per group and 30 broilers per replicate. Broilers in control group were fed a corn-soybean meal basal diet, and the others in citrus extract group were fed the basal diets supplemented with 10 mg/kg citrus extract. The experiment was lasted for 63 days. At the end of the experiment, six broilers were selected from each replicate for transport stress test by 2×3 factors experimental design, and slaughtering and sampled after transportation for 0, 2 and 4 h, respectively. The results showed as follows:1) there was significant interaction between dietary treatment and transportation time on body weight loss of rapidly-growing yellow-feathered broilers (P<0.05). Compared with control group, body weight loss in citrus extract group was significantly decreased after transportation for 2 and 4 h (P<0.05). Transportation time significantly affected body weight loss, body weight loss was increased with the extension of transportation time (P<0.05). 2) Compared with control group, dietary citrus extract significantly increased breast muscle rate and leg muscle rate of rapidly-growing yellow-feathered broilers after transportation for 0, 2 and 4 h (P<0.05). 3) Compared with control group, the content of malondialdehyde (MDA) of breast muscle of rapidly-growing yellow-feathered broilers in citrus extract group was significantly decreased after transportation for 0, 2 and 4 h (P<0.05), and the activity of glutathione peroxidase (GSH-Px) of breast muscle in citrus extract group was significantly increased after transportation for 2 and 4 h (P<0.05). Transport time significantly affected the content of MDA and the activity of GSH-Px, the content of MDA was significantly increased and the activity of GSH-Px was significantly decreased with the extension of transport time (P<0.05). 4) There was significant interaction between dietary treatment and transportation time on 45 min brightness (L*45 min) and 45 min redness (a*45 min) of rapidly-growing yellow-feathered broilers (P<0.05). Compared with control group, L*45 min of breast muscle in citrus extract group was significantly decreased after transportation for 0, 2 and 4 h (P<0.05), while a*45 min was significantly increased (P<0.05). Transportation time significantly affected L*45 min and a*45 min, L*45 min was significantly increased and a*45 min was significantly decreased with the extension of transportation time (P<0.05). Compared with control group, the shear force of breast muscle of rapidly-growing yellow-feathered broilers in citrus extract group was significantly decreased after transportation for 0, 2 and 4 h (P<0.05). Transportation time significantly affected shear force of breast muscle, shear force was significantly increased with the extension of transportation time (P<0.05). It is concluded that transportation stress significantly affects body weight loss and meat quality of yellow-feathered broilers, and dietary citrus extract can reduce body weight loss of yellow-feathered broilers after transportation, improve antioxidant capacity of breast muscle, improve meat quality, and alleviate the impact of transportation stress on yellow-feathered broilers.

Key words： broilers; citrus extract; transport stress; body weight loss; slaughtering performance; antioxidant capacity; meat quality

参考文献

[1] MIRANDA-DE LA LAMA G C, VILLARROEL M, MARÍA G A.Livestock transport from the perspective of the pre-slaughter logistic chain:a review[J].Meat Science, 2014, 98(1):9-20.  
[2] 李贞明, 余苗, 崔艺燕, 等.柑橘提取物对肉鸡运输应激后血浆生化指标、激素水平和抗氧化功能的影响[J].中国畜牧兽医, 2020, 47(9):2816-2823. LI Z M, YU M, CUI Y Y, et al.Effects of citrus extract on plasma biochemical indexes, hormone levels and antioxidant function of broilers after transport stress[J].China Animal Husbandry & Veterinary Medicine, 2020, 47(9):2816-2823.(in Chinese)
[3] GOU Z, ABOUELEZZ K F M, FAN Q, et al.Physiological effects of transport duration on stress biomarkers and meat quality of medium-growing yellow broiler chickens[J].Animal, 2021, 15(2):100079.
[4] PERAI A H, KERMANSHAHI H, MOGHADDAM H N, et al.Effects of chromium and chromium+vitamin C combination on metabolic, oxidative, and fear responses of broilers transported under summer conditions[J].International Journal of Biometeorology, 2015, 59(4):453-462.  
[5] RAPA S F, DI IORIO B R, CAMPIGLIA P, et al.Inflammation and oxidative stress in chronic kidney disease-potential therapeutic role of minerals, vitamins and plant-derived metabolites[J].International Journal of Molecular Sciences, 2019, 21(1):263.
[6] 刘延鑫, 孙宇, 李业亮, 等.黄芪多糖缓解肉牛短途运输应激的效果研究[J].中国畜牧兽医, 2017, 44(1):87-93. LIU Y X, SUN Y, LI Y L, et al.Effect of Astragalus polysaccharides on short-distance transport stress in beef cattle[J].China Animal Husbandry & Veterinary Medicine, 2017, 44(1):87-93.(in Chinese)
[7] 陈凯凯, 张成, 赵晓惠, 等.饲粮添加L-茶氨酸对运输应激肉鸡胴体性状、器官指数和肉品质的影响[C]//中国畜牧兽医学会2018年学术年会禽病学分会第十九次学术研讨会论文集, 南宁:中国畜牧兽医学, 2018:223. CHEN K K, ZHANG C, ZHAO X H, et al.Effects of dietary L-theanine supplementation on carcass traits, organ index and meat quality of broilers under transportation stress[C]//Proceedings of the 19th Symposium of Avian Disease Branch of Chinese Society of Animal Husbandry and Veterinary in 2018, Nanning:Chinese Association of Animal Science and Veterinary Medicine, 2018:223.(in Chinese)
[8] 赵泮峰.连翘提取物缓解不同应激原诱导的肉仔鸡生长抑制及氧化损伤的研究[D].博士学位论文.北京:中国农业大学, 2016. ZHAO P F.Effects of Forsythia suspense extract on alleviating growth inhibition and oxidative injury in broilers induced by different stimuli[D].Ph.D.Thesis.Beijing:China Agricultural University, 2016.(in Chinese)
[9] 刘文静, 潘葳.柑橘属果实中类黄酮成分的高效液相色谱标准化测定方法[J].中国农学通报, 2018, 34(32):157-164. LIU W J, PAN W.Determining flavonoids in citrus fruits using HPLC standardized method[J].Chinese Agricultural Science Bulletin, 2018, 34(32):157-164.(in Chinese)
[10] YU M, LI Z M, CHEN W D, et al.Dietary supplementation with citrus extract altered the intestinal microbiota and microbial metabolite profiles and enhanced the mucosal immune homeostasis in yellow-feathered broilers[J].Frontiers in Microbiology, 2019, 10:2662.
[11] 李贞明, 余苗, 崔艺燕, 等.柑橘提取物对黄羽肉鸡器官指数、屠宰性能、肉品质和肌肉抗氧化指标的影响[J].动物营养学报, 2019, 31(8):3794-3800. LI Z M, YU M, CUI Y Y, et al.Effects of citrus extract on organ indices, slaughter performance, meat quality and muscle antioxidant indices of yellow-feather broilers[J].Chinese Journal of Animal Nutrition, 2019, 31(8):3794-3800.(in Chinese)
[12] 张林, 张海军, 岳洪源, 等.运输应激对畜禽的影响及其应对措施[J].家畜生态学报, 2009, 30(2):106-109. ZHANG L, ZHANG H J, YUE H Y, et al.Effects of transportation stress on livestock and alleviating strategy[J]. Journal of Domestic Animal Ecology, 2009, 30(2):106-109.(in Chinese)
[13] 王国艮, 吴鑫, 孟庆翔, 等.运输应激对反刍动物机体功能及肉品质影响的研究进展[J].中国畜牧兽医, 2017, 44(3):755-760. WANG G G, WU X, MENG Q X, et al.Research progress on effect of transport stress on ruminants body function and meat quality[J].China Animal Husbandry & Veterinary Medicine, 2017, 44(3):755-760.(in Chinese)
[14] 谢京君, 李前勇, 张德志, 等.运输应激对动物机体影响的研究现状[J].四川畜牧兽医, 2014(1):35-37. XIE J J, LI Q Y, ZHANG D Z, et al.Research the effect of transport stress on animal organisms[J].Sichuan Animal & Veterinary Sciences, 2014(1):35-37.(in Chinese)
[15] KARAMAN M.Effect of transport time on body performance of broilers during transit to slaughter house[J].Journal of Animal and Veterinary Advances, 2009, 8(8):1555-1557.
[16] ZHANG L, LI J L, GAO T, et al.Effects of dietary supplementation with creatine monohydrate during the finishing period on growth performance, carcass traits, meat quality and muscle glycolytic potential of broilers subjected to transport stress[J].Animal, 2014, 8(12):1955-1962.  
[17] PICCIONE G, CASELLA S, GIANNETTO C, et al.Oxidative stress associated with road transportation in ewes[J].Small Ruminant Research, 2013, 112(1/3):235-238.
[18] BAI K W, HUANG Q, ZHANG J F, et al.Supplemental effects of probiotic Bacillus subtilis fmbJ on growth performance, antioxidant capacity, and meat quality of broiler chickens[J].Poultry Science, 2017, 96(1):74-82.  
[19] BHATTACHARYYA A, CHATTOPADHYAY R, MITRA S, et al.Oxidative stress:an essential factor in the pathogenesis of gastrointestinal mucosal diseases[J].Physiological Reviews, 2014, 94(2):329-354.  
[20] GUO S W, MA J X, XING Y Y, et al.Artemisia annua L. aqueous extract as an alternative to antibiotics improving growth performance and antioxidant function in broilers[J].Italian Journal of Animal Science, 2020, 19(1):399-409.  
[21] WANG S H, ZHANG L, LI J L, et al.Effects of dietary marigold extract supplementation on growth performance, pigmentation, antioxidant capacity and meat quality in broiler chickens[J].Asian-Australasian Journal of Animal Sciences, 2017, 30(1):71-77.
[22] ZHANG C, WANG L, ZHAO X H, et al.Dietary resveratrol supplementation prevents transport-stress-impaired meat quality of broilers through maintaining muscle energy metabolism and antioxidant status[J].Poultry Science, 2017, 96(7):2219-2225.  
[23] WANG C C, CHANG S C, INBARAJ B S, et al.Isolation of carotenoids, flavonoids and polysaccharides from Lycium barbarum L. and evaluation of antioxidant activity[J].Food Chemistry, 2010, 120(1):184-192.  
[24] LIU Q, TANG G Y, ZHAO C N, et al.Antioxidant activities, phenolic profiles, and organic acid contents of fruit vinegars[J].Antioxidants, 2019, 8(4):78.
[25] 张舒翔, 康大成, 张丽丽, 等.运输时间对扬州鹅应激程度和肉品质的影响[J].食品科学技术学报, 2018, 36(6):66-72. ZHANG S X, KANG D C, ZHANG L L, et al.Effects of transport time on stress level and meat quality in Yangzhou goose[J].Journal of Food Science and Technology, 2018, 36(6):66-72.(in Chinese)
[26] ZOU F, MAO X Q, WANG N, et al.Astragalus polysaccharides alleviates glucose toxicity and restores glucose homeostasis in diabetic states via activation of AMPK[J].Acta Pharmacologica Sinica, 2009, 30(12):1607-1615.  
[27] 楚维斌, 史彬林, 张鹏飞, 等.艾蒿水提物对肉仔鸡生长性能、屠宰性能和肉品质的影响[J].饲料工业, 2015, 36(10):6-9. CHU W B, SHI B L, ZHANG P F, et al.Effect of wormwood extract on growth performance, slaughter performance and meat quality of broilers[J].Feed Industry, 2015, 36(10):6-9.(in Chinese)
[28] JIANG Z Y, JIANG S Q, LIN Y C, et al.Effects of soybean isoflavone on growth performance, meat quality, and antioxidation in male broilers[J].Poultry Science, 2007, 86(7):1356-1362.  
[29] ZHANG C, YANG L, ZHAO X H, et al.Effect of dietary resveratrol supplementation on meat quality, muscle antioxidative capacity and mitochondrial biogenesis of broilers[J].Journal of the Science of Food and Agriculture, 2018, 98(3):1216-1221.  
[30] 岳远西, 史彬林, 赵启龙, 等.黑沙蒿提取物对肉仔鸡生长性能和屠宰性能及肉品质的影响[J].中国畜牧杂志, 2015, 51(17):39-43. YUE Y X, SHI B L, ZHAO Q L, et al.Effects of Artemisia ordosica extract on growth performance, slaughter performance and meat quality of broilers[J].Chinese Journal of Animal Science, 2015, 51(17):39-43.(in Chinese)
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