动物营养学报 >

2021 , Vol. 33 >Issue 10: 5690 - 5700

DOI: https://doi.org/10.3969/j.issn.1006-267x.2021.10.028

反刍与草食动物营养与饲料 RUMINANT AND HERBIVORE NUTRITION AND FEED

饲粮能量水平对干奶期奶牛瘤胃发酵、消化代谢及血浆生化指标的影响

  • 王富伟 ,
  • 何雅琴 ,
  • 郑宇慧 ,
  • 苏华维 ,
  • 李胜利
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  • 1. 中国农业大学动物科学技术学院, 动物营养国家重点实验室, 北京市生鲜乳质量安全工程技术研究中心, 北京 100193;
    2. 北京首农畜牧发展有限公司, 北京 100076
王富伟(1985-),男,山东泰安人,硕士,从事动物营养与饲料科学专业研究。E-mail:wang_fuwei@foxmail.com

收稿日期: 2021-03-09

  网络出版日期: 2021-10-16

基金资助

国家重点研发计划（2018YFD0501600）；现代奶牛高效安全养殖技术应用与示范

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Effects of Dietary Energy Levels on Rumen Fermentation, Digestion and Metabolism and Plasma Biochemical Parameters of Dairy Cows during Dry Period

  • WANG Fuwei ,
  • HE Yaqin ,
  • ZHENG Yuhui ,
  • SU Huawei ,
  • LI Shengli
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  • 1. Beijing Engineering Technology Research Center of Raw Milk Quality and Safety Control, State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China;
    2. Beijing Sunlon Livestock Development Company Limited, Beijing 100076, China

Received date: 2021-03-09

  Online published: 2021-10-16

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摘要

本试验旨在研究饲粮能量水平对干奶期奶牛瘤胃发酵、消化代谢及血浆生化指标的影响。选择4头体重（BW）、体况评分（BCS）、预产期相近并装有永久性瘤胃瘘管的干奶期健康奶牛，采用4×4拉丁方设计，分别饲喂4种不同能量水平（产奶净能分别为5.46、5.78、6.11和6.43 MJ/kg DM）的饲粮。结果表明：随着饲粮能量水平的提高，干奶期奶牛瘤胃pH有降低的趋势（0.05<P<0.10），氨态氮含量有增加的趋势（0.05<P<0.10），丙酸含量显著增加（P<0.05），乙酸/丙酸显著降低（P<0.05）；干奶期奶牛干物质表观消化率、粪尿氮总排泄量和氮沉积显著增加（P<0.05），氮利用率有提高的趋势（0.05<P<0.10），而中性洗涤纤维（NDF）和酸性洗涤纤维（ADF）表观消化率以及粪尿氮总排泄量占摄入氮的比例有下降的趋势（0.05<P<0.10）；干奶期奶牛血浆胰岛素含量显著升高（P<0.05），葡萄糖含量有升高的趋势（0.05<P<0.10），而非酯化脂肪酸含量显著降低（P<0.05）。由此可见，干奶期奶牛饲喂高能量水平饲粮可以促进采食，提高营养物质表观消化率、氮利用率和肝糖异生前体物质的产量，改善干奶期奶牛能量平衡状况。

关键词： 干奶期; 奶牛; 饲粮能量; 瘤胃发酵; 消化代谢; 血液指标

本文引用格式

王富伟 , 何雅琴 , 郑宇慧 , 苏华维 , 李胜利 . 饲粮能量水平对干奶期奶牛瘤胃发酵、消化代谢及血浆生化指标的影响[J]. 动物营养学报, 2021 , 33(10) : 5690 -5700 . DOI: 10.3969/j.issn.1006-267x.2021.10.028

Abstract

The aim of this study is to investigate the effects of dietary energy levels on rumen fermentation, digestion and metabolism and plasma biochemical parameters of dairy cows during dry period. Four ruminally cannulated Holstein cows with similar body weight, body condition score and expected date of childbirth were used in a 4×4 Latin square design, and fed different energy levels (net energy for lactating were 5.46, 5.78, 6.11 and 6.43 MJ/kg DM, respectively) diets. The results showed that with the dietary energy level increasing, the rumen pH tended to decrease of dairy cows during dry period (0.05<P<0.10), the ammonia nitrogen content tended to increase (0.05<P<0.10), the propionate content significantly increased (P<0.05), and the acetate/propionate significantly decreased (P<0.05); the dry matter apparent digestibility, total nitrogen excretion of feces and urine and nitrogen retention of dairy cows during dry period significantly increased (P<0.05), the nitrogen utilization efficiency tended to increase (0.05<P<0.10), and the neutral detergent fiber (NDF) and acid detergent fiber (ADF) apparent digestibilities and ratio of total nitrogen excretion of feces and urine to nitrogen intake tended to decrease (0.05<P<0.10); the plasma insulin content of dairy cows during dry period significantly increased (P<0.05), the glucose content tended to increase (0.05<P<0.10), and the nonesterified fatty acids content significantly decreased (P<0.05). In conclusion, feeding high energy level diet for dairy cows during dry period can promote the feed intake, increase nutrient apparent digestibility, nitrogen utilization rate and hepatic gluconeogenesis precursor substances yield, and improve the energy balance of dairy cows during dry period.

Key words： dry period; dairy cows; dietary energy; rumen fermentation; digestion and metabolism; blood parameters

参考文献

[1] WILKENS M R, NELSON C D, HERNANDEZ L L, et al.Symposium review:transition cow calcium homeostasis-health effects of hypocalcemia and strategies for prevention[J].Journal of Dairy Science, 2020, 103(3):2909-2927.  
[2] ARSHAD U, ZENOBI M G, STAPLES C R, et al.Meta-analysis of the effects of supplemental rumen-protected choline during the transition period on performance and health of parous dairy cows[J].Journal of Dairy Science, 2020, 103(1):282-300.  
[3] MC S, O'MARA F P, RATH M, et al.Effects of different transition diets on dry matter intake, milk production, and milk composition in dairy cows[J].Journal of Dairy Science, 2003, 86:2397-2408.
[4] DANN H M, LITHERLAND N B, UNDERWOOD J P, et al.Prepartum nutrient intake has minimal effects on postpartum dry matter intake, serum nonesterified fatty acids, liver lipid and glycogen contents, and milk yield[J].Journal of Dairy Science, 2003, 86(Suppl.1):106.
[5] RUKKWAMSUK T, WENSING T, GEELEN M J H.Effect of overfeeding during the dry period on regulation of adipose tissue metabolism in dairy cows during the periparturient period[J].Journal of Dairy Science, 1998, 81:2904-2911.
[6] INGVARTSEN K L, ANDERSEN J B.Integration of metabolism and intake regulation:a review focusing on periparturient animals[J].Journal of Dairy Science, 2000, 83:1573-1597.
[7] CONTRERAS L L, RYAN C M, OVERTON T R.Effects of dry cow grouping strategy and prepartum body condition score on performance and health of transition dairy cows[J].Journal of Dairy Science, 2004, 87:517-523.
[8] HUANG W M, TIAN Y J, WANG Y J, et al.Effect of reduced energy density of close-up diets on dry matter intake, lactation performance and energy balance multiparous Holstein cows[J].Journal of Animal Science and Biotechnology, 2015, 6(1):47-54.  
[9] Dancy K M, RIBEIRO E S, DEVRIES T J.Effect of dietary transition at dry off on the behavior and physiology of dairy cows[J].Journal of Dairy Science, 2019, 102(5):4387-4402.  
[10] BAUR F J, ENSMINGER L G.The association of official analytical chemists (AOAC)[J]. Journal of the American Oil Chemists Society, 1977, 54(4):171-172.  
[11] VAN SOEST P J, ROBERTSON J B, LEWIS B A.Methods for dietary fiber, neutral detergent fiber, and non-starch polysaccharides in relation to animal nutrition[J].Journal of Dairy Science, 1991, 74:3593-3597.
[12] BRODERICK G A, KANG J H.Automated simultaneous determination of ammonia and total amino acids in ruminal fluid and in vitro media[J].Journal of Dairy Science, 1980, 63:64-75.
[13] ERWIN E S, MARCOAND G J, EMERY E M.Volatile fatty acid analysis of food and rumen fluid by gas chromatography[J].Journal of Dairy Science, 1961, 44:1768-1777.
[14] CAO Z J, MA M, YAN X Y, et al.A simple urine-collecting apparatus and method for cows and heifers[J].Journal of Dairy Science, 2009, 92:5224-5228.
[15] BRODERICK G A, CLAYTON M K.A Statistical evaluation of animal and nutritional factors influencing concentrations of milk urea nitrogen[J].Journal of Dairy Science, 1997, 80:2964-2971.
[16] NRC.Nutrient requirements of dairy cattle[S].Washington, D.C.:National Academy Press, 2001.
[17] VAN HOUTER M F J.The production and metabolism of volatile fatty acids by ruminants fed roughages:a review[J].Animal Feed Science and Technology, 1993, 43:189-225.
[18] 杨晓东, 李常瑞, 冀国珍, 等.不同精粗比饲粮中添加复合益生菌及其培养物对绵羊瘤胃发酵指标的影响[J].饲料研究, 2020, 43(11):16-22. YANG X D, LI C R, JI G Z, et al.Effects of adding compound probiotics and their cultures in diets with different concentrate-to-forage ratios on rumen fermentation indexes of sheep[J].Feed Research, 2020, 43(11):16-22.(in Chinese)
[19] 秦正君, 王若勇, 时国峰, 等.饲粮精粗比对奶牛瘤胃发酵及生产性能的影响[J].畜牧兽医杂志, 2018, 37(1):83-85. QIN Z J, WANG R Y, SHI G F, et al.The effect of dietary concentrate ratio on the rumen fermentation and production performance of dairy cows[J].Journal of Animal Science and Veterinary Medicine, 2018, 37(1):83-85.(in Chinese)
[20] SATTER L D, SLYTER L L.Effect of ammonia concentration on rumen microbial protein production in vitro[J].British Journal of Nutrition, 1974, 32:199-208.
[21] PRESTON H, LENG D.Automated simultaneous determination of ammonia and total amino acid in ruminal fluids and in vitro media[J].Journal of Dairy Science, 1987, 63:64-67.
[22] 涂瑞, 苗建军, 彭忠利, 等.不同精粗比饲粮中添加小肽对牦牛瘤胃体外发酵特性的影响[J].草业学报, 2020, 29(3):78-88. TU R, MIAO J J, PENG Z L, et al.The effect of adding small peptides in diets with different concentrate to crude ratios on the in vitro fermentation characteristics of yak's rumen[J].Acta Prataculturae Sinica, 2020, 29(3):78-88.(in Chinese)
[23] HUANG W M, TIAN Y J, WU Z H, et al.Reduced energy density of close-up diets decrease ruminal pH and increase concentration of volatile fatty acids postpartum in Holstein cows[J].Animal Science Journal, 2017, 88(11):1700-1708.  
[24] 黄文明.围产前期日粮能量水平对奶牛能量代谢和瘤胃适应性影响的研究[D].硕士学位论文.北京:中国农业大学, 2014:6-7. HUANG W M.Study on the effect of dietary energy level during prenatal period on energy metabolism and rumen adaptability of dairy cows[D].Master's Thesis.Beijing:China Agricultural University, 2014:6-7.(in Chinese)
[25] MURPHY M M, KERLIND A, HOLTENIUS K.Rumen fermentation in lactating cows selected for milk fat content fed two forage to concentrate ratios with hay or silage[J].Journal of Dairy Science, 2000, 83:756-764.
[26] ZHOU X Q, ZHANG Y D, ZHAO M, et al.Effect of dietary energy source and level on nutrient digestibility, rumen microbial protein synthesis, and milk performance in lactating dairy cows[J].Journal of Dairy Science, 2015, 98:1-9.
[27] COBLENTZ W K, ESSER N M, HOFFMAN P C, et al.Growth performance and sorting characteristics of corn silage alfalfa haylage diets with or without forage dilution offered to replacement Holstein dairy heifers[J].Journal of Dairy Science, 2015, 98:8018-8034.
[28] MOULD F L, ORSKOV E R, MANN S O.Associative effects of mixed feeds.Ⅰ.Effects of type and level of supplementation and the influence of the rumen fluid pH on cellulolysis in vivo and dry matter digestion of various roughages[J].Animal Feed Science and Technology, 1983, 10:15-30.
[29] SU H W, AKINS M S, NANCY M, et al.Effects of feeding alfalfa stemlage or wheat straw for dietary energy dilution on nutrient intake and digestibility, growth performance, and feeding behavior of Holstein dairy heifers[J].Journal of Animal Science, 2017, 100:7106-7115.
[30] JANOVICK N A, BOISCLAIR Y R, DRACKLEY J K.Prepartum dietary energy intake affects metabolism and health during the periparturient period in primiparous and multiparous holstein cows[J].Journal of Dairy Science, 2011, 94, 1385-1400.
[31] GRAUGNARD D E, BIONAZ M, TREVISI E, et al.Blood immunometabolic indices and polymorphonuclear neutrophil function in peripartum dairy cows are altered by level of dietary energy prepartum[J].Journal of Animal Science, 2012, 95:1749-1758.
[32] HAISAN J, INABU Y, SHI W, et al.Effects of pre- and postpartum dietary starch content on productivity, plasma energy metabolites, and serum inflammation indicators of dairy cows[J].Journal of Animal Science, 2021, 102(4):3082-3096.
[33] LAW R A, YOUNG F J, PATTERSON D C, et al.Effect of precalving and postcalving dietary energy level on performance and blood metabolite concentrations of dairy cows throughout lactation[J].Journal of Dairy Science, 2011, 94:808-823.
[34] FREDERICK Y O, AHMAD R, SIMON H, et al.Blood metabolite profile in Holstein-Friesian cows fed diets varying in dry matter and metabolizable energy density during early lactation[J].Journal of Dairy Science, 2018, 27(5):1191-1197.
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