动物营养学报 >

2021 , Vol. 33 >Issue 10: 5760 - 5770

DOI: https://doi.org/10.3969/j.issn.1006-267x.2021.10.035

反刍与草食动物营养与饲料 RUMINANT AND HERBIVORE NUTRITION AND FEED

复合植物提取物对湘东黑山羊胃肠道游离氨基酸含量及其转运载体相对表达量的影响

  • 徐琪翔 ,
  • 曹文豪 ,
  • 罗双贵 ,
  • 谢静静 ,
  • 吕小康 ,
  • 朱晋佳 ,
  • 吴祎程 ,
  • 周传社 ,
  • 谭支良
展开
  • 1. 中国科学院亚热带农业生态研究所, 中国科学院亚热带农业生态过程重点实验室, 畜禽养殖污染控制与资源化技术国家工程实验室, 动物营养生理与代谢过程湖南省重点实验室, 长沙 410125;
    2. 中国科学院大学, 北京 100049;
    3. 湖南先伟实业有限公司, 长沙 410016
徐琪翔(1995-),男,四川巴中人,硕士研究生,研究方向为反刍家畜营养。E-mail:xuqixiang_123@163.com

收稿日期: 2021-03-05

  网络出版日期: 2021-10-16

基金资助

国家自然科学基金（U20A2057，31772632）；国家重点研发计划（2018YFD0501903，2017YFD0500500）

收起

Effects of Plant Extract Mixture on Free Amino Acids Content and Relative Expression Level of Their Transporters in Gastrointestinal Tract of Xiangdong Black Goat

  • XU Qixiang ,
  • CAO Wenhao ,
  • LUO Shuanggui ,
  • XIE Jingjing ,
  • LYU Xiaokang ,
  • ZHU Jinjia ,
  • WU Yicheng ,
  • ZHOU Chuanshe ,
  • TAN Zhiliang
Expand
  • 1. Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, CAS Key Laboratory for Agro-Ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Science, Changsha 410125, China;
    2. College of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing 100049, China;
    3. Hunan Xianwei Industrial Co., Ltd., Changsha 410016, China

Received date: 2021-03-05

  Online published: 2021-10-16

Supported by

 

Fold

摘要

本试验旨在研究饲粮中添加复合植物（含杜仲叶、迷迭香叶、枇杷叶和柿子叶）提取物对湘东黑山羊胃肠道游离氨基酸含量及其转运载体相对表达量的影响。选择体重相近[（9.82±0.83） kg]的健康雌性湘东黑山羊20头，随机分为2组，每组10头。对照组饲喂基础饲粮，试验组在基础饲粮中添加0.15%的复合植物提取物，试验期56 d。结果表明：与对照组相比，1）饲粮添加复合植物提取物可极显著提高湘东黑山羊瘤胃游离天冬氨酸（Asp）、谷氨酸（Glu）、甘氨酸（Gly）、丙氨酸（Ala）和脯氨酸（Pro）的含量（P<0.01），显著提高游离苏氨酸（Thr）的含量（P<0.05）；瘤胃黏膜钙敏感受体（CASR）基因的相对表达量上调了68.6%（P<0.01）；2）饲粮添加复合植物提取物可极显著提高湘东黑山羊回肠游离Gly、Ala和精氨酸（Arg）的含量（P<0.01），显著提高游离缬氨酸（Val）的含量（P<0.05）；回肠黏膜溶质载体家族1成员1（SLC1A1）基因的相对表达量上调了162.6%（P<0.01），溶质载体家族1成员3（<SLC1A3）和溶质载体家族38成员2（SLC38A2）基因的相对表达量分别上调了27.1%和34.3%（P<0.05）；3）饲粮添加复合植物提取物可极显著降低盲肠游离Thr、Glu、Gly、酪氨酸（Tyr）和苯丙氨酸（Phe）的含量（P<0.01），同时可显著降低丝氨酸（Ser）、Ala、Val和赖氨酸（Lys）的含量（P<0.05）；盲肠黏膜SLC1A1基因的相对表达量下调了77.2%（P<0.01），溶质载体家族7成员1（SLC7A1）基因的相对表达量上调了29.2%（P<0.01）；溶质载体家族3成员2（SLC3A2）和SLC38A2基因的相对表达量分别上调了21.5%和34.3%（P<0.05）。综上所述，复合植物提取物能够显著提高湘东黑山羊胃肠道部分游离氨基酸的含量，同时调控胃肠道黏膜氨基酸转运载体的相对表达。

关键词： 湘东黑山羊; 复合植物提取物; 游离氨基酸; 氨基酸转运载体

本文引用格式

徐琪翔 , 曹文豪 , 罗双贵 , 谢静静 , 吕小康 , 朱晋佳 , 吴祎程 , 周传社 , 谭支良 . 复合植物提取物对湘东黑山羊胃肠道游离氨基酸含量及其转运载体相对表达量的影响[J]. 动物营养学报, 2021 , 33(10) : 5760 -5770 . DOI: 10.3969/j.issn.1006-267x.2021.10.035

Abstract

The study was conducted to explore the effects of plant extract mixture (PEM) (Eucommia ulmoides, rosemary, loquat and persimmon leaves) on free amino acids contents and relative expression levels of amino acid transporters in the rumen and intestine of Xiangdong black goats. In total, twenty healthy female-goats with similar weight of (9.82±0.83) kg were selected and equally randomly assigned into 2 groups, control group and experimental group (basic diets with 0.15% PEM). The experiment lasted for 56 days. The results showed that compared with control group, 1) adding PEM to the diet could significantly increase the rumen free aspartic acid (Asp), glutamic acid (Glu), glycine (Gly), alanine (Ala) and proline (Pro) contents of Xiangdong black goats (P<0.01), the content of free threonine (Thr) was significantly increased (P<0.05); the relative expression level of rumen mucosal calcium-sensing receptor (CASR) gene was significantly up-regulated by 68.6% (P<0.01); 2) adding PEM to the diet could significantly increase the contents of free Gly, Ala and arginine (Arg) in the ileum of Xiangdong black goats (P<0.01) and significantly increase the content of free valine (Val) (P<0.05); the relative expression level of ileal mucosal solute carrier family 1 member 1 (SLC1A1) gene was significantly up-regulated by 162.6% (P<0.01), the relative expression levels of solute carrier family 1 member 3 (<SLC1A3) and solute carrier family 38 member 2 (SLC38A2) genes were significantly increased by 27.1% and 34.3% (P<0.05), respectively; 3) adding PEM to the diet could significantly reduce the cecal free Thr, Glu, Gly, tyrosine (Tyr) and phenylalanine (Phe) contents (P<0.01), and at the same time, could significantly reduce serine (Ser) and Ala (P<0.05); the relative expression level of SLC1A1 gene in cecal mucosa was significantly down-regulated by 77.2% (P<0.01), and the relative expression level of solute carrier family 7 member 1 (SLC7A1) gene was significantly up-regulated by 29.2% (P<0.01); the relative expression level of solute carrier family 3 member 2 (SLC3A2) and SLC38A2 genes were significantly up-regulated by 21.5% and 34.3% (P<0.05). In summary, the PEM can significantly increase the contents of free amino acids in the rumen and intestines of Xiangdong black goats, while regulating the relative expression of amino acid transporters in the rumen and intestinal mucosa.

Key words： Xiangdong black goat; plant extract mixture; free amino acid; amino acid transporter

参考文献

[1] KHIAOSA-ARD R, ZEBELI Q.Meta-analysis of the effects of essential oils and their bioactive compounds on rumen fermentation characteristics and feed efficiency in ruminants[J].Journal of Animal Science, 2013, 91(4):1819-1830.  
[2] 田志梅, 崔艺燕, 杜宗亮, 等.抗生素替代物在畜禽养殖中的研究及应用进展[J].动物营养学报, 2020, 32(4):1516-1525. TIAN Z M, CUI Y Y, DU Z L, et al.Advances in researches and applications of antibiotic alternatives in livestock breeding[J].Chinese Journal of Animal Nutrition, 2020, 32(4):1516-1525.(in Chinese)
[3] 武卫党, 王泽, 魏滋鸿, 等.糖复康宁提取物对SGLT2的抑制作用及降糖作用研究[J].药物评价研究, 2018, 41(6):992-996. WU W D, WANG Z, WEI Z H, et al.Inhibition of tangfu kangning extract to SGLT2 and its antidiabetic effects[J].Drug Evaluation Research, 2018, 41(6):992-996.(in Chinese)
[4] ZHU M Q, SUN R C.Eucommia ulmoides oliver:a potential feedstock for bioactive products[J].Journal of Agricultural and Food Chemistry, 2018, 66(22):5433-5438.  
[5] DE OLIVEIRA MONTESCHIO J, DE SOUZA K A, VITAL A C P, et al.Clove and rosemary essential oils and encapsuled active principles (eugenol, thymol and vanillin blend) on meat quality of feedlot-finished heifers[J].Meat Science, 2017, 130:50-57.
[6] 彭芳, 宋泽和, 谢谦, 等.熊果酸对动物肠道黏膜屏障功能的调控作用及可能机制[J].动物营养学报, 2020, 32(6):2513-2520. PENG F, SONG Z H, XIE Q, et al.Ursolic acid:regulation on intestinal mucosal barrier function and possible mechanisms[J].Chinese Journal of Animal Nutrition, 2020, 32(6):2513-2520.(in Chinese)
[7] 沈晓静, 赵红梅, 赵蕾, 等.齐墩果酸研究进展[J].广州化工, 2019, 47(24):16-19. SHEN X J, ZHAO H M, ZHAO L, et al.Research progress on oleanolic[J].Guangzhou Chemical Industry, 2019, 47(24):16-19.(in Chinese)
[8] 王绿阳, 李英英, 李忠鑫, 等.动物胃肠道氨基酸感应与转运[J].动物营养学报, 2020, 32(7):3031-3038. WANG L Y, LI Y Y, LI Z X, et al.Amino acid sensing and transport in animal gastrointestinal tract[J].Chinese Journal of Animal Nutrition, 2020, 32(7):3031-3038.(in Chinese)
[9] 曾黎明, 谭碧娥, 肖昊, 等.氨基酸转运载体介导的氨基酸感应信号研究进展[J].中国科学(生命科学), 2012, 42(9):699-708. ZENG L M, TAN B E, XIAO H, et al.Amino acid sensing signaling induced by amino acid transporters[J].Science in China(Life Sciences), 2012, 42(9):699-708.(in Chinese)
[10] WU J, ZHANG X L, WANG R, et al.Replacing corn grain with corn gluten feed:effects on the rumen microbial protein synthesis, functional bacterial groups and epithelial amino acid chemosensing in growing goats[J].Animal Feed Science and Technology, 2020, 270:114684.
[11] RIST V, WEISS E, EKLUND M, et al.Impact of dietary protein on microbiota composition and activity in the gastrointestinal tract of piglets in relation to gut health:a review[J].Animal, 2013, 7(7):1067-1078.  
[12] 孙雅君.绵羊胃肠道游离氨基酸与小肽分布及其转运载体相关基因表达的研究[D].硕士学位论文.杨凌:西北农林科技大学, 2017. SUN Y J.Study on distribution of free amino acid and small peptide and the expression of related transporter genes in gastrointestinal tract of sheeps[D].Master's Thesis.Yangling:Northwest A & F University, 2017.(in Chinese)
[13] LEIBHOLZ J.The absorption of amino acids from the rumen of the sheep.Ⅰ.The loss of amino acids from solutions placed in the washed rumen in vivo[J].Australian Journal of Agricultural Research, 1971, 22(4):639-645.  
[14] RÉMOND D, BERNARD L, PONCET C.Amino acid flux in ruminal and gastric veins of sheep:effects of ruminal and omasal injections of free amino acids and carnosine[J].Journal of Animal Science, 2000, 78(1):158-166.  
[15] MATTHEWS J C, Jr, WEBB K E.Absorption of L-carnosine, L-methionine, and L-methionylglycine by isolated sheep ruminal and omasal epithelial tissue[J].Journal of Animal Science, 1995, 73(11):3464-3475.  
[16] 闫磊.日粮营养水平对前胃上皮几种氨基酸转运载体及Na+/H+交换蛋白mRNA表达的影响[D].硕士学位论文.南京:南京农业大学, 2010. YAN L.Effects of dietary nutrition level on mRNA abundace of amino acid trasporters and Na+/H+ exchanges in epithelium of forestomach[D].Master's Thesis.Nanjing:Nanjing Agricultural University, 2010.(in Chinese)
[17] 周英昊.陕北白绒山羊碱性氨基酸转运载体基因cDNA克隆及时空表达分析[D].硕士学位论文.杨凌:西北农林科技大学, 2015. ZHOU Y H.CDNA cloning and space-time expression of cationic amino acid transporters gene in Shanbei white cashmere goat[D].Master's Thesis.Yangling:Northwest A&F University, 2015.(in Chinese)
[18] PORTUNE K J, BEAUMONT M, DAVILA A M, et al.Gut microbiota role in dietary protein metabolism and health-related outcomes:the two sides of the coin[J].Trends in Feed Science and Technology, 2016, 57(Part B):213-232.
[19] FISCHBACH M A, SONNENBURG J L.Eating for two:how metabolism establishes interspecies interactions in the gut[J].Cell Host & Microbe, 2011, 10(4):336-347.  
[20] FICHMAN Y, GERDES S Y, KOVÁCS H, et al.Evolution of proline biosynthesis:enzymology, bioinformatics, genetics, and transcriptional regulation[J].Biological Reviews of the Cambridge Philosophical Society, 2015, 90(4):1065-1099.  
[21] TRIVEDI V, GUPTA A, JALA V R, et al.Crystal structure of binary and ternary complexes of serine hydroxymethyltransferase from Bacillus stearothermophilus:insights into the catalytic mechanism[J].The Journal of Biological Chemistry, 2002, 277(19):17161-17169.  
[22] 许静, 俎昊辰, 丛玉艳.植物提取物调控反刍动物甲烷排放量的研究进展[J].畜牧与兽医, 2020, 52(7):127-130. XU J, ZU H C, CONG Y Y.Progress in research on plant extracts in regulation of methane emissions from ruminants[J].Animal Husbandry & Veterinary Medicine, 2020, 52(7):127-130.(in Chinese)
[23] HAID D, WIDMAYER P, BREER H.Nutrient sensing receptors in gastric endocrine cells[J].Journal of Molecular Histology, 2011, 42(4):355-364.  
[24] HEBERT S C, CHENG S, GEIBEL J.Functions and roles of the extracellular Ca2+-sensing receptor in the gastrointestinal tract[J].Cell Calcium, 2004, 35(3):239-247.  
[25] 何林波, 张丽, 刘妍妍, 等.CaSR基因的研究进展[J].山东畜牧兽医, 2019, 40(8):93-95. HE L B, ZHANG L, LIU Y Y, et al.Research progress of CaSR gene[J].Shandong Journal of Animal Science and Veterinary Medicine, 2019, 40(8):93-95.(in Chinese)
[26] BRÖER S, FAIRWEATHER S J.Amino acid transport across the mammalian intestine[J].Comprehensive Physiology, 2018, 9(1):343-373.
[27] SILK D B, GRIMBLE G K, REES R G.Protein digestion and amino acid and peptide absorption[J].Proceedings of the Nutrition Socirty, 1985, 44(1):63-72.  
[28] 徐晓锋, 金曙光, 欧宇, 等.不同蛋白源日粮对绵羊瘤胃氨基酸和肽释放影响的研究[J].内蒙古农业大学学报(自然科学版), 2004, 25(2):9-12. XU X F, JIN S G, OU Y, et al.The effects of different protein sources on concentration of amino acids and peptide in the rumen of sheep[J].Journal of Inner Mongolia Agricultural University(Natural Science Edition), 2004, 25(2):9-12.(in Chinese)
[29] CHEN J L, ZHENG P, ZHANG C, et al.Benzoic acid beneficially affects growth performance of weaned pigs which was associated with changes in gut bacterial populations, morphology indices and growth factor gene expression[J].Journal of Animal Physiology and Animal Nutrition, 2017, 101(6):1137-1146.  
[30] HU Q L, LI S S, ZHANG Y W, et al.Phytosterols on growth performance, antioxidant enzymes and intestinal morphology in weaned piglets[J].Journal of the Science of Food Agriculture, 2017, 97(13):4629-4634.  
[31] CHEN Y Q, RONG L, QIAO J O.Anti-inflammatory effects of Panax notoginseng saponins ameliorate acute lung injury induced by oleic acid and lipopolysaccharide in rats[J].Molecular Medicine Reports, 2014, 10(3):1400-1408.  
[32] 徐琪翔, 曹文豪, 罗双贵, 等.复合植物提取物对湘东黑山羊生长性能和肌肉脂肪酸组成的影响[J].动物营养学报, 2021, 33(4):2146-2157. XU Q X, CAO W H, LUO S G, et al.Effects of plant extract mixture on the growth performance and fatty acid profiles of the longissimusmuscle of goat[J].Chinese Journal of Animal Nutrition, 2021, 32(4):2146-2157.(in Chinese)
[33] KANDASAMY P, GYIMESI G, KANAI Y, et al.Amino acid transporters revisited:new views in health and disease[J].Trends in Biochemical Sciences, 2018, 43(10):752-789.  
[34] HE F, WU C L, LI P, et al.Functions and signaling pathways of amino acids in intestinal inflammation[J].BioMed Research International, 2018, 2018:9171905.
[35] HE L Q, YANG H S, HOU Y Q, et al.Effects of dietary L-lysine intake on the intestinal mucosa and expression of CAT genes in weaned piglets[J].Amino Acids, 2013, 45(2):383-391.  
[36] MORALES A, BARRERA M A, ARAIZA A B, et al.Effect of excess levels of lysine and leucine in wheat-based, amino acid-fortified diets on the mRNA expression of two selected cationic amino acid transporters in pigs[J].Journal of Animal Physiology and Animal Nutrition, 2013, 97(2):263-270.  
[37] DEMIRTAS I, ERENLER R, ELMASTAS M, et al.Studies on the antioxidant potential of flavones of Allium vineale isolated from its water-soluble fraction[J].Food Chemistry, 2013, 136(1):34-40.  
[38] PORTUNE K J, BEAUMONT M, DAVILA A M, et al.Gut microbiota role in dietary protein metabolism and health-related outcomes:the two sides of the coin[J].Trends in Food Science and Technology, 2016, 57(Part B):213-232.
Options
文章导航

/

〈 〉

    联系我们

  • 地址：北京市海淀区圆明园西路2号
    　　　中国农业大学动科动医大楼153室
  • 邮编：100193
  • 电话：010－62817823
  • 网址：http://www.chinajan.com
  • E-mail：cjan@chinajan.com
京公网安备 11010802041926号    京ICP备17050989号-2