动物营养学报 >

2021 , Vol. 33 >Issue 10: 5802 - 5808

DOI: https://doi.org/10.3969/j.issn.1006-267x.2021.10.039

分子与细胞营养 MOLECULAR AND CELLULAR NUTRITION

脂多糖刺激24 h后对仔猪腓肠肌炎症和蛋白质降解相关基因与miRNA表达的影响

  • 范佳俊 ,
  • 黄兴法 ,
  • 汪洋 ,
  • 李先根 ,
  • 万志成 ,
  • 刘玉兰 ,
  • 康萍
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  • 武汉轻工大学动物营养与饲料科学湖北省重点实验室, 武汉 430023
范佳俊(1995-),男,河南信阳人,硕士研究生,从事动物营养研究。E-mail:1416686220@qq.com

收稿日期: 2021-03-30

  网络出版日期: 2021-10-16

基金资助

湖北省自然科学基金创新群体项目（2019CFA015）；湖北省自然科学基金面上项目（2019CFB831）；湖北省自然科学基金面上项目（2018CFB527）

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Effects of Lipopolysaccharide on Inflammation and Protein Degradation-Related Genes and miRNA Expression in Gastrocnemius of Piglets after 24 h Stimulation

  • FAN Jiajun ,
  • HUANG Xingfa ,
  • WANG Yang ,
  • LI Xiangen ,
  • WAN Zhicheng ,
  • LIU Yulan ,
  • KANG Ping
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  • Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, China

Received date: 2021-03-30

  Online published: 2021-10-16

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摘要

本试验旨在探讨脂多糖（LPS）刺激24 h后，与仔猪腓肠肌炎症和蛋白质降解相关基因与miRNA的表达变化。试验选用12头健康的28日龄断奶的杜×长×大三元杂交仔猪[体重为（7.09±0.90） kg]，随机分为对照组（n=6）和LPS组（n=6），所有试验猪自由采食和饮水，预试期为15 d。在试验第15天，LPS组仔猪腹腔注射100 μg/kg BW的LPS，并于注射24 h后麻醉屠宰，对照组仔猪则腹腔注射等量的无菌生理盐水，并于注射后立即麻醉屠宰，取腓肠肌样品待测。结果显示：LPS处理24 h后，腓肠肌炎症细胞因子白细胞介素-1β（IL-1β）和肿瘤坏死因子-α（TNF-α），以及Toll样受体4（TLR4）信号通路关键基因TLR4、骨髓分化因子88（MyD88）、白细胞介素受体相关激酶1（IRAK1） mRNA的表达量显著上升（P<0.05），且核因子-κB （NF-κB）和蛋白质降解相关基因叉头转录因子4（FOXO4） mRNA的表达量也显著上升（P<0.05）。LPS处理24 h后，腓肠肌miR-145的表达量显著上升（P<0.05），而miR-30b、miR-155、miR-132、miR-130a和miR-146a的表达量显著下降（P<0.05）。综上所述，LPS处理24 h后的仔猪腓肠肌损伤可能是通过TLR4信号通路介导FOXO4表达上调所引起的。miRNA可能参与LPS刺激后24 h腓肠肌炎症和蛋白质降解反应的调控。

关键词： 脂多糖; 腓肠肌; 断奶仔猪; 炎症; 蛋白质降解; miRNA

本文引用格式

范佳俊 , 黄兴法 , 汪洋 , 李先根 , 万志成 , 刘玉兰 , 康萍 . 脂多糖刺激24 h后对仔猪腓肠肌炎症和蛋白质降解相关基因与miRNA表达的影响[J]. 动物营养学报, 2021 , 33(10) : 5802 -5808 . DOI: 10.3969/j.issn.1006-267x.2021.10.039

Abstract

This experiment was conducted to investigate the expression changes of genes and miRNA related to inflammation and protein degradation in gastrocnemius of weaned piglets challenged by lipopolysaccharide (LPS) at 24 h. Twelve healthy Duroc×Landrace×Yorkshine 28-day-old weaned piglets[body weight:(7.09±0.90) kg] were selected, and randomly divided into 2 groups:control group (n=6) and LPS group (n=6). All piglets were allowed ad libitum access to water and feed. On day 15 of experiment, the piglets in LPS group were injected with LPS (100 μg/kg BW) and sacrificed at 24 h post-injection. The control group received the same amount of sterile saline, and sacrificed immediately after the injection. Gastrocnemius samples were selected for testing. The results showed as follows:at 24 h after LPS treatment, the mRNA expression levels of inflammatory factors interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α), as well as the key genes of Toll-like receptor 4 (TLR4) signaling pathway including TLR4, myeloid differentiation factor 88 (MyD88) and interleukin receptor-associated kinase 1 (IRAK1) were significantly increased in gastrocnemius (P<0.05), and the mRNA expression levels of nuclear factor-κB (NF-κB) and protein degradation-related gene forkhead box O4 (FOXO4) were also significantly increased (P<0.05). Moreover, miR-145 expression level in gastrocnemius was significantly increased (P<0.05), and the expression levels of miR-30b, miR-155, miR-132, miR-130a and miR-146a were significantly decreased at 24 h after LPS treatment (P<0.05). The results indicate that the gastrocnemius injury can be caused by the up-regulation of FOXO4 expression mediated by TLR4 signaling pathway at 24 h after LPS treatment. The miRNA may be involved in muscle inflammation and protein degradation at 24 h after LPS stimulation.

Key words： lipopolysaccharide; gastrocnemius; weaned piglet; inflammation; protein degradation; miRNA

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