动物营养学报 >

2021 , Vol. 33 >Issue 10: 5809 - 5816

DOI: https://doi.org/10.3969/j.issn.1006-267x.2021.10.040

分子与细胞营养 MOLECULAR AND CELLULAR NUTRITION

骆驼乳清蛋白对热应激大鼠肝脏炎症及高迁移率族蛋白B1/Toll样受体4/核转录因子-κB信号通路的调节

  • 乌恩吉雅 ,
  • 杜冬华 ,
  • 马雪妮 ,
  • 哈斯苏荣
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  • 1. 内蒙古农业大学兽医学院, 农业农村部动物疾病临床诊疗技术重点实验室, 呼和浩特 010018;
    2. 河北北方学院动物科技学院, 张家口 075131;
    3. 内蒙古骆驼研究院, 阿拉善 750300
乌恩吉雅(1995-),男,内蒙古锡林郭勒人,硕士研究生,从事兽医药理学与毒理学研究。E-mail:1727298241@qq.com

收稿日期: 2021-03-24

  网络出版日期: 2021-10-16

基金资助

内蒙古自然科学基金项目（2020MS03011）；国家自然科学基金项目（32060815）

收起

Effects of Camel Whey Protein on Hepatic Inflammation and High Mobility Group Protein B1/Toll Like Receptor 4/Nuclear Factor-κB Signaling Pathway in Heat Stressed Rats

  • WUENJiya ,
  • DU Donghua ,
  • MA Xueni ,
  • HASISurong
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  • 1. Key Laboratory of Clinical Diagnosis and Treatment Technology in Animal Disease, Ministry of Agriculture and Rural Affairs, College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, China;
    2. College of Animal Science and Technology, Hebei North University, Zhangjiakou 075131, China;
    3. Inner Mongolia institute of Camel Research, Alashan 750300, China

Received date: 2021-03-24

  Online published: 2021-10-16

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摘要

本试验旨在研究骆驼乳清蛋白（CWP）对热应激（HS）所致大鼠肝脏炎症及相关信号通路的调节作用。选取6周龄SD大鼠，适应性饲养2周后随机分为6组，每组设置5个重复，每个重复6只，试验期22 d。正常对照组（Control组）饲喂基础饲粮；CWP对照组（CWP组）每天灌服400 mg/kg BW CWP；HS致肝脏损伤组（HS组）饲喂基础饲粮并从第15天开始每天进行2 h HS处理，连续8 d；CWP低、中、高剂量干预组（L、M和H组）每天灌服100、200和400 mg/kg BW CWP，且从第15天开始每天进行2 h HS处理，连续8 d。酶联免疫吸附试验（ELISA）检测肝脏肿瘤坏死因子-α（TNF-α）、白细胞介素-1β（IL-1β）、白细胞介素-6（IL-6）、白细胞介素-8（IL-8）和白细胞介素-10（IL-10）含量，免疫组织化学及免疫印迹（Western blotting）方法检测肝脏高迁移率族蛋白B1（HMGB1）、Toll样受体4（TLR4）和磷酸化核转录因子κB p65（p-NF-κB p65）蛋白表达。结果表明：1）与HS组相比，400 mg/kg的CWP干预显著降低了HS大鼠肝脏TNF-α、IL-1β、IL-6及IL-8含量（P<0.05），显著提高了IL-10含量（P<0.05）。2）与HS组相比，CWP以剂量依赖方式极显著降低了HS大鼠肝脏HMGB1蛋白核转位（P<0.01），但对其总蛋白表达水平没有显著影响（P>0.05），同时极其显著降低了TLR4蛋白表达水平（P<0.001），极显著降低了p-NF-κB p65蛋白水平（P<0.01）及核转位。与Control组相比，400 mg/kg的CWP干预效果最好。由此可见，HS前灌服400 mg/kg的CWP可调节HS大鼠肝脏HMGB1/TLR4/NF-κB信号通路并缓解炎症反应。

关键词： 骆驼乳清蛋白; 大鼠; 肝脏; 炎症反应; 信号通路; 热应激

本文引用格式

乌恩吉雅 , 杜冬华 , 马雪妮 , 哈斯苏荣 . 骆驼乳清蛋白对热应激大鼠肝脏炎症及高迁移率族蛋白B1/Toll样受体4/核转录因子-κB信号通路的调节[J]. 动物营养学报, 2021 , 33(10) : 5809 -5816 . DOI: 10.3969/j.issn.1006-267x.2021.10.040

Abstract

This experiment was aimed to investigate the regulatory effects of camel whey protein (CWP) on hepatic inflammatory and relative signaling pathways in rats induced by heat stress (HS). SD rats at 6 weeks of age were selected and randomly divided into six groups after 2 weeks of adaptive feeding, with five replicates in each group, six in each replicate, and test period was 22 d. The normal control group (Control group) was fed a basal diet. The CWP control group (CWP group) fed 400 mg/kg BW CWP daily. HS induced liver injury group (HS group) was fed the basal diet and subjected to 2 h HS treatment daily from day 15 for 8 d. CWP low-, medium-, and high-dose intervention groups (L, M and H groups) were received 100, 200, and 400 mg/kg BW CWP daily and subjected to 2 h HS treatment daily for 8 consecutive days starting at day 15. Hepatic tumor necrosis factor-α (TNF-α), interleukins-1β (IL-1β), interleukins-6 (IL-6), interleukins-8 (IL-8), and interleukins-10 (IL-10) contents were measured by enzyme linked immunosorbent assay (ELISA), and hepatic high mobility group protein B1 (HMGB1), Toll like receptor 4 (TLR4), and phosphorylated nuclear factor-κB (p-NF-κB p65) protein expression was detected by immunohistochemistry and Western blotting. The results showed as follows:1) compared with the HS group, CWP intervention at 400 mg/kg effectively reduced hepatic TNF-α, IL-1β, IL-6 and IL-8 contents (P<0.05) and increased IL-10 content in HS rats (P<0.05). 2) Compared with the HS group, CWP reduced the nuclear translocation of HMGB1 protein in the liver of HS rats in a dose-dependent manner (P<0.01), but had no effect on the total protein expression (P>0.05), while it extremely significantly reduced TLR4 protein expression (P<0.001), significantly reduced p-NF-κB p65 protein expression (P<0.01) and nuclear translocation. Compared with the Control group, CWP intervention at 400 mg/kg had the best effect. Thus, it is concluded that CWP at 400 mg/kg administered before HS modulates the hepatic HMGB1/TLR4/NF-κB signaling pathway and alleviates inflammatory responses in HS rats.

Key words： camel whey protein; rat; liver; inflammatory response; signaling pathway; heat stress

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