动物营养学报 >

2021 , Vol. 33 >Issue 10: 5827 - 5837

DOI: https://doi.org/10.3969/j.issn.1006-267x.2021.10.042

实验方法与实验动物 EXPERIMENTAL METHOD AND ANIMAL

植物炭黑对采食玉米赤霉烯酮污染饲粮的大鼠生长性能和生殖系统的影响

  • 刘淑杰 ,
  • 邓波 ,
  • 吴杰 ,
  • 徐子伟 ,
  • 马倩倩
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  • 浙江省农业科学院畜牧兽医研究所, 杭州 310021
刘淑杰(1978-),女,内蒙古扎兰屯人,副研究员,博士,主要从事动物健康养殖与分子生物学研究。E-mail:liushujie78@126.com

收稿日期: 2021-03-20

  网络出版日期: 2021-10-16

基金资助

浙江省重点研发计划项目（2021C02007）；浙江省科技厅资助项目（2016C02054）；国家现代农业产业技术体系建设专项资金资助（CARS-36）；国家青年科学基金项目（C170105/31501966）

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Effects of Vegetable Carbon Black on Growth Performance and Reproductive System of Rats Fed Zearalenone Contaminated Diet

  • LIU Shujie ,
  • DENG Bo ,
  • WU Jie ,
  • XU Ziwei ,
  • MA Qianqian
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  • Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

Received date: 2021-03-20

  Online published: 2021-10-16

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摘要

本文通过研究在玉米赤霉烯酮（ZEN）污染的饲粮中添加不同剂量的植物炭黑对大鼠生长性能和生殖系统的影响，旨在评价植物炭黑对ZEN的吸附效果和对大鼠健康的改善作用，为其用于畜牧生产及饲料行业提供依据。试验采用完全随机设计，选用64只体重约为150 g的5~6周龄无特定病原体（SPF）级雌性SD大鼠，随机分为4个组，每组16个重复，每个重复1只。4个组分别为：1）空白对照组，饲喂基础饲粮；2）负对照组，饲喂基础饲粮+25 mg/kg ZEN；3）低剂量植物炭黑组，饲喂基础饲粮+25 mg/kg ZEN+1.0 g/kg植物炭黑；4）高剂量植物炭黑组，饲喂基础饲粮+25 mg/kg ZEN+1.5 g/kg植物炭黑。试验预试期7 d，正试期28 d。结果表明：1）与空白对照组相比，负对照组大鼠第1~14天平均日增重（ADG）显著降低（P<0.05），第1~28天ADG极显著降低（P<0.01），分别降低了26.06%和28.45%；负对照组大鼠第1~14天料重比（F/G）显著升高（P<0.05），第15~28天和第1~28天F/G极显著升高（P<0.01），分别升高了33.08%、28.21%和40.73%。与负对照组相比，高剂量植物炭黑组大鼠第15~28天和第1~28天F/G显著降低（P<0.05），分别降低了15.90%和20.89%。2）与空白对照组相比，负对照组大鼠第14和28天血清碱性磷酸酶（ALP）活性极显著升高（P<0.01），第28天血清谷丙转氨酶（GPT）活性显著升高（P<0.05），第28天血清谷草转氨酶（GOT）活性极显著升高（P<0.01），分别升高了37.67%、44.17%、42.55%和37.75%。与负对照组相比，高剂量植物炭黑组大鼠第14天血清ALP活性极显著降低（P<0.01），第28天血清GPT和GOT活性显著降低（P<0.05），分别降低了22.48%、24.48%和20.27%。3）与空白对照组相比，负对照组大鼠第14天血清促卵泡素（FSH）浓度极显著升高（P<0.01），第28天血清FSH浓度显著升高（P<0.05），分别升高了20.40%和19.86%；负对照组大鼠第14和28天血清雌二醇（E2）浓度极显著降低（P<0.01），分别降低了19.30%和17.59%；负对照组大鼠第14天血清促黄体素（LH）浓度极显著降低（P<0.01），第28天血清LH浓度显著降低（P<0.05），分别降低了46.74%和25.63%。与负对照组相比，低剂量植物炭黑组大鼠第14天血清LH浓度极显著升高（P<0.01），升高了35.20%；高剂量植物炭黑组大鼠第14天血清FSH浓度极显著降低（P<0.01），第28天血清FSH浓度显著降低（P<0.05），分别降低了28.94%和19.38%；高剂量植物炭黑组大鼠第14和28天血清E2浓度极显著升高（P<0.01），第14天血清LH浓度极显著升高（P<0.01），分别升高了29.98%、31.50%和71.94%。4）卵巢组织苏木精-伊红（HE）染色结果显示，负对照组卵巢皮质部可见萎缩，原始卵泡、初级卵泡和初级卵母细胞明显较空白对照组减少，低剂量植物炭黑组和高剂量植物炭黑组卵巢组织形态较负对照组明显改善。由此可见，植物炭黑具有吸附ZEN的能力，饲粮添加植物炭黑具有缓解ZEN导致的大鼠生长性能下降和繁殖系统紊乱的作用；推荐植物炭黑适宜添加剂量为1.5 g/kg。

关键词： 玉米赤霉烯酮; 植物炭黑; 吸附剂; 大鼠; 生长性能; 生殖系统

本文引用格式

刘淑杰 , 邓波 , 吴杰 , 徐子伟 , 马倩倩 . 植物炭黑对采食玉米赤霉烯酮污染饲粮的大鼠生长性能和生殖系统的影响[J]. 动物营养学报, 2021 , 33(10) : 5827 -5837 . DOI: 10.3969/j.issn.1006-267x.2021.10.042

Abstract

In this study, the effects of different doses of dietary vegetable carbon black in the zearalenone (ZEN) contaminated diet on the growth performance and reproductive system of rats were studied, so as to evaluate the adsorption effects of vegetable carbon black on ZEN and the improvement effects on the health of rats, and to provide a basis for its use in animal husbandry and feed industry. In a completely randomized design, sixty-four 5- to 6-week-old specific pathogen free (SPF) female SD rats with a body weight of 150 g were randomly divided into 4 groups with 16 replicates per group and 1 rat per replicate. The four groups were as follows:1) the blank control group, and the rats were fed a basal diet; 2) the negative control group, and the rats were fed the basal diet+25 mg/kg ZEN; 3) the low-dose vegetable carbon black group, and the rats were fed the basal diet+25 mg/kg ZEN+1.0 g/kg vegetable carbon black; 4) the high-dose vegetable carbon black group, and the rats were fed the basal diet+25 mg/kg ZEN+1.5 g/kg vegetable carbon black. The experimental period lasted for 28 days after 7-day adaption. The results showed as follows:1) compared with the blank control group, the average daily gain (ADG) of rats in the negative control group was significantly decreased by 26.06% from days 1 to 14 (P<0.05), and was extremely significantly decreased by 28.45% from days 1 to 28 (P<0.01); the ratio of feed to gain (F/G) in the negative control group was significantly increased by 33.08% from days 1 to 14 (P<0.05), and was extremely significantly increased by 28.21% and 40.73% from days 15 to 28 and days 1 to 28 (P<0.01), respectively. Compared with the negative control group, the F/G of rats in the high-dose vegetable carbon black group was significantly decreased by 15.90% and 20.89% from days 15 to 28 and days 1 to 28 (P<0.05), respectively. 2) Compared with the blank control group, the serum alkaline phosphatase (ALP) activity of rats in the negative control group was extremely significantly increased by 37.67% and 44.17% on days 14 and 28 (P<0.01), the serum glutamic-pyruvic transaminase (GPT) activity was significantly increased by 42.55% on day 28 (P<0.05), and the serum glutamic-oxalacetic transaminase (GOT) activity was extremely significantly increased by 37.75% on day 28 (P<0.01). Compared with the negative control group, the serum ALP activity of rats in the high-dose vegetable carbon black group was extremely significantly decreased by 22.48% on day 14 (P<0.01), and the serum GPT and GOT activities were significantly decreased by 24.48% and 20.27% on day 28 (P<0.05), respectively. 3) Compared with the blank control group, the serum follicle stimulating hormone (FSH) concentration of rats in the negative control group was extremely significantly increased by 20.40% on day 14 (P<0.01), and was significantly increased by 19.86% on day 28 (P<0.05); the serum estradiol (E2) concentration of rats in the negative control group was extremely significantly decreased by 19.30% and 17.59% on days 14 and 28 (P<0.01); the serum luteinizing hormone (LH) concentration of rats in the negative control group was extremely significantly decreased by 46.74% on day 14 (P<0.01), and was significantly decreased by 25.63% on day 28 (P<0.05). Compared with the negative control group, the serum LH concentration of rats in the low-dose vegetable carbon black group was extremely significantly increased by 35.20% on day 14 (P<0.01); the serum FSH concentration in the high-dose vegetable carbon black group was extremely significantly decreased by 28.94% on day 14 (P<0.01), and was significantly decreased by 19.38% on day 28 (P<0.05); the serum E2 concentration of rats in high-dose vegetable carbon black group was extremely significantly increased by 29.98% and 31.50% on days 14 and 28 (P<0.01), and the serum LH concentration was extremely significantly increased by 71.94% on day 14 (P<0.01). 4) Hematoxylin-eosin (HE) staining of the ovarian tissue showed that atrophy was observed in the ovarian cortex in the negative control group, and the numbers of primordial follicles, primary ovarian follicles and primary oocytes were significantly decreased compared with the blank control group. The morphology of the ovarian tissue in the low-dose and high-dose of vegetable carbon black groups was significantly improved compared with the negative control group. In conclusion, the vegetable carbon black has the ability to adsorb ZEN, and dietary plant carbon black can alleviate the growth performance decline and reproductive system disorder caused by ZEN in rats. The recommended dosage of vegetable carbon black is 1.5 g/kg.

Key words： zearalenone; vegetable carbon black; adsorbent; rats; growth performance; reproductive system

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