动物营养学报 >

2021 , Vol. 33 >Issue 10: 5895 - 5907

DOI: https://doi.org/10.3969/j.issn.1006-267x.2021.10.048

研究简报 SHORT COMMUNICATION

低锌饲粮中添加载锌蒙脱石对肉鸡组织微量元素含量、肝脏酶活性及空肠锌转运蛋白基因表达的影响

  • 秦士贞 ,
  • 王海波 ,
  • 武真邑 ,
  • 龚莉媛 ,
  • 裴文刚 ,
  • 车育彦 ,
  • 杨敏敏 ,
  • 史兆国
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  • 甘肃农业大学动物科学技术学院, 兰州 730070
秦士贞(1988-),女,甘肃平川人,讲师,博士,研究方向为家禽分子营养。E-mail:qinshizhen@163.com

收稿日期: 2021-03-20

  网络出版日期: 2021-10-16

基金资助

国家自然科学基金（03119023）；省青年科技基金计划（20GR5RA013）；甘肃农业大学人才专项经费（2017RCZX-18）；甘肃省现代农业产业技术体系猪鸡产业岗位（GARS-ZJ-2）

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Effects of Low Zinc Diets Supplemented with Zinc-Montmorillonite on Trace Element Contents in Tissue, Enzyme Activity in Liver and Zinc Transporter Protein Gene Expression in Jejunum of Broilers

  • QIN Shizhen ,
  • WANG Haibo ,
  • WU Zhenyi ,
  • GONG Liyuan ,
  • PEI Wengang ,
  • CHE Yuyan ,
  • YANG Minmin ,
  • SHI Zhaoguo
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  • College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China

Received date: 2021-03-20

  Online published: 2021-10-16

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摘要

本试验旨在研究低锌饲粮中添加载锌蒙脱石（Zn-MMT）对肉鸡组织微量元素含量、肝脏含锌酶活性、抗氧化能力和空肠锌转运蛋白基因表达的影响。随机选择1日龄健康科宝公雏240只，按体重一致原则随机分成5组，每组6个重复，每个重复8只仔鸡。对照组（CK组）饲喂不额外添加锌源的玉米-豆粕型基础饲粮（低锌饲粮），4个试验组分别在低锌饲粮中添加20、40、60和80 mg/kg的Zn-MMT （均以锌含量计算）替代载体。试验鸡自由采食和饮水，试验期为42 d。结果表明：1）相比CK组，低锌饲粮中添加Zn-MMT显著提高21日龄全血和42日龄胫骨中的锌含量（P<0.05）；并显著降低21日龄胫骨中铁以及42日龄胫骨、胰腺中锰的含量（P<0.05），其中添加40 mg/kg Zn-MMT能显著提高42日龄全血中铁、铜、锌和胸肌、腿肌中锰的含量（P<0.05）。其中，Zn-MMT的添加量达到40 mg/kg时，显著提高21日龄胰腺中锰、胫骨中锌以及42日龄肝脏、胫骨中锌的含量（P<0.05），但添加60 mg/kg的Zn-MMT能显著降低21日龄胸肌中的铜及42日龄胰腺中铁、胸肌中锌的含量（P<0.05），并显著提高胰腺中锌的含量（P<0.05）。同时，添加80 mg/kg的Zn-MMT显著降低腿肌中锰、胫骨和胰腺中铜的含量（P<0.05）。2）相比CK组，低锌饲粮中添加Zn-MMT对肉鸡肝脏碱性磷酸酶（ALP）、苹果酸脱氢酶（MDH）活性及乳酸脱氢酶（LDH）、铜锌超氧化物歧化酶（CuZn-SOD）含量，丙二醛（MDA）含量及总抗氧化能力（T-AOC）均无显著影响（P>0.05）。3）相比CK组，低锌饲粮中添加Zn-MMT显著提高空肠锌转运载体-5（ZnT-5）基因相对表达量（P<0.05）；添加20和40 mg/kg的Zn-MMT显著提高空肠二价金属离子转运体1（DMT1）基因相对表达量（P<0.05），同时，添加40、60和80 mg/kg的Zn-MMT显著提高空肠金属硫蛋白3（MT3）基因相对表达量（P<0.05）；添加60和80 mg/kg的Zn-MMT显著提高空肠金属硫蛋白（MT）、锌转运载体-2（ZnT-2）基因相对表达量（P<0.05）。由此可见，低锌饲粮中添40 mg/kg的Zn-MMT能显著提高肉鸡42日龄空肠DMT1、MT3、ZnT-5转运体蛋白的表达，并提高肝脏、胫骨中锌和全血中的铁、铜、锌的含量及腿肌中锰和胸肌中锰、铜的含量。但锌在肝脏、胫骨、胰腺、全血中的含量随Zn-MMT添加量的增加而增加，从而影响铁、锰、铜在组织中的含量。

关键词： 肉鸡; 载锌蒙脱石; 微量元素; 肝脏酶活性; 锌转运蛋白表达

本文引用格式

秦士贞 , 王海波 , 武真邑 , 龚莉媛 , 裴文刚 , 车育彦 , 杨敏敏 , 史兆国 . 低锌饲粮中添加载锌蒙脱石对肉鸡组织微量元素含量、肝脏酶活性及空肠锌转运蛋白基因表达的影响[J]. 动物营养学报, 2021 , 33(10) : 5895 -5907 . DOI: 10.3969/j.issn.1006-267x.2021.10.048

Abstract

This experiment was conducted to investigate the effects of low zinc diets supplemented with zinc montmorillonite (Zn-MMT) on tissue trace element contents, liver zinc-containing enzymes, antioxidant capacity and jejunum zinc transporter protein gene expression of broilers. A total of two hundred and forty 1-day-old male Cobb broilers with similar body weight were randomly assigned into 5 groups with 6 replicates per group and 8 broilers per replicate. The broilers in the control group (CK group) were fed a corn-soybean meal basal diet (low zine diet), and those in the four experimental groups were fed the low zine diet supplemented with 20, 40, 60 and 80 mg/kg Zn-MMT (in terms of zinc content), respectively. The chickens were free to feed and drink, and the experiment lasted for 42 days. The results showed as follows:1) compared with the CK group, low zinc diets supplemented with Zn-MMT significantly increased the zinc content in whole blood at 21 days of age (P<0.05), and in tibia at 42 days of age (P<0.05). At the same time, adding Zn-MMT significantly decreased the content of iron in tibia at 21 days of age and manganese content in tibia and pancreas at 42 days of age (P<0.05). Among them, adding 40 mg/kg Zn-MMT significantly increased the contents of iron, copper and zinc in whole blood and the contents of manganese in breast muscle and leg muscle at 42 days of age (P<0.05). When the supplemental level of Zn-MMT was more than 40 mg/kg, it significantly increased the content of manganic in pancreas and zinc in tibia at 21 days of age (P<0.05),and also increased the zinc content in liver and tibia at 42 days of age (P<0.05). However, adding 60 mg/kg of Zn-MMT significantly reduced the copper content in breast muscle at 21 days of age (P<0.05), and decreased the content of iron in pancreas and zinc content in breast muscle at 42 days of age (P<0.05), but increased the zinc content in pancreas (P<0.05). At the same time, adding 80 mg/kg significantly decreased the content of manganic in leg muscle and copper in tibia and pancreas (P<0.05). 2) Compared with CK group, low zinc diets supplemented with Zn-MMT had no significant effects on liver alkaline phosphatase (ALP) and malate dehydrogenase (MDH) activities, malondialdehyde (MDA) content, total antioxidant capacity (T-AOC), lactate dehydrogenase (LDH) and copper zinc superoxide dismutase (CuZn-SOD) contents of broilers at 21 and 42 days of age (P>0.05). 3) Compared with CK group, low zinc diets supplemented with Zn-MMT significantly increased the relative expression level of zinc transporter-5 (ZnT-5) gene (P<0.05). Moreover, adding 20 and 40 mg/kg Zn-MMT significantly increased the relative expression levels of divalent metal ion transporter 1 (DMT1) gene, and adding 40, 60 and 80 mg/kg significantly increased the relative expression level of anti-metallothionein 3 (MT3) gene in jejunum (P<0.05). At the same time, adding 60 and 80 mg/kg Zn-MMT significantly increased the relative expression levels of metallothionein (MT) and zinc transporter-2 (ZnT-2) genes in jejunum (P<0.05). To sum up, low zinc supplementation of 40 mg/kg Zn-MMT can significantly increase the expression of DMT1, MT3, ZnT-5 in jejunum, and promote the content of zinc in liver and tibia as well as the contents of manganese and copper in breast muscle. It also promotes the contents of iron, copper and zinc in the whole blood and manganese in the leg muscles. However, the content of zinc in liver, tibia, pancreas and whole blood increase with the addition of Zn-MMT, but affect the contents of iron, copper and manganese in tissues.

Key words： broiler; zinc-loaded montmorillonite; trace elements; liver enzyme activity; zinc transporter protein expression

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