禽营养与饲料 POULTRY NUTRITION AND FEED

发育期间乳鸽血清代谢物及代谢通路的变化

  • 安勇 ,
  • 计峰 ,
  • 王雪敏 ,
  • 王铮 ,
  • 张帅
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  • 1. 河北工程大学生命科学与食品工程学院, 邯郸 056000;
    2. 北京市农林科学院畜牧兽医研究所, 北京 100089
安勇(1995-),男,山东淄博人,硕士研究生,研究方向为饲料科学。E-mail:982356929@qq.com

收稿日期: 2021-04-23

  网络出版日期: 2021-11-10

基金资助

北京市农林科学院改革与发展课题(XMS201906);北京市科委国家现代农业科技城产业培育与成果惠民项目(Z171100001517003);北京市农林科学院畜牧兽医研究所课题(201705)

Changes of Serum Metabolites and Pathways during Development of Squabs

  • AN Yong ,
  • JI Feng ,
  • WANG Xuemin ,
  • WANG Zheng ,
  • ZHANG Shuai
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  • 1. College of Life Sciences and Food Engineering, Hebei University of Engineering, Handan 056000, China;
    2. Institute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100089, China

Received date: 2021-04-23

  Online published: 2021-11-10

Supported by

 

摘要

本试验应用液相色谱-质谱联用(LC-MS)技术分析乳鸽发育期间血清代谢物的变化。选取14日龄、体重(398.33±43.28) g与21日龄、体重(449.17±49.44) g的健康乳鸽各6只,翅静脉采血,分离血清作为样本,采用基于LC-MS非靶向代谢组学技术进行分析,根据变量投影重要度(VIP)和独立样本t检验筛选出差异代谢物,并作通路富集分析。结果显示:在2个日龄乳鸽血清样本中共检测到11 538个特征代谢峰,正、负离子模式下共鉴定出694种代谢物。经搜库(自建库、Metlin、HMDB等数据库)定性分析,共筛选出71种差异代谢物,其中有49种差异代谢物丰度显著上调(P<0.05),22种差异代谢物丰度显著下调(P<0.05)。KEGG通路富集共获得29条代谢通路,其中14条代谢通路差异显著(P<0.05),主要为甘油脂代谢、醚脂代谢、糖基磷脂酰肌醇(GPI)-锚定生物合成等。乳鸽血清S-腺苷蛋氨酸、L-棕榈酰肉碱、硬脂酰肉碱和磷脂类代谢物的含量随着日龄增加而显著增加(P<0.05)。上述结果有助于了解乳鸽发育期生理状态的变化,为生产中更好地满足其营养需要提供参考。

本文引用格式

安勇 , 计峰 , 王雪敏 , 王铮 , 张帅 . 发育期间乳鸽血清代谢物及代谢通路的变化[J]. 动物营养学报, 2021 , 33(11) : 6203 -6212 . DOI: 10.3969/j.issn.1006-267x.2021.11.021

Abstract

The changes of serum metabolites during the development of squabs were analyzed with the technique of liquid chromatography-mass spectrometry. Six 14-day-old healthy squabs with the body weight of (398.33±43.28) g and six 21-day-old healthy squabs with the body weight of (449.17±49.44) g were selected for wing venous blood sampling, and serum was prepared and analyzed with non-target metabolomics based on the technique of LC-MS. The differential metabolites were screened out according to the variable importance in projection (VIP) and independent sample t-test, and their pathway enrichments were analyzed. The results showed that a total of 11 538 characteristic metabolic peaks were detected in the serum of 14- and 21-day-old squabs, and 694 metabolites identified in the positive and negative ion models. After the qualitative analysis of database (self-built database, Metlin, HMDB etc.), a total of 71 differential metabolites were screened, of which 49 differential metabolites were significantly up-regulated (P<0.05), and 22 differential metabolites were significantly down-regulated (P<0.05). The KEGG pathway enrichment resulted in 29 metabolic pathways, 14 of which were significantly different (P<0.05), mainly glycerophospholipid metabolism, ether lipid metabolism, glycosylphosphatidylinositol (GPI)-anchor biosynthesis etc. The contents of serum S-adenosylmethionine, L-palmitoylcarnitine, stearoylcarnitine and some metabolites of phospholipids were significantly rosed with the increase of age of squabs (P<0.05). The above data are helpful to understand the changes of the physiological state of the squabs during the development and provide references for better satisfying their nutritional needs in production.

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