反刍与草食动物营养与饲料 RUMINANT AND HERBIVORE NUTRITION AND FEED

青藏高原那曲和当雄区域高山草甸藏嵩草营养价值比较研究

  • 鲍宇红 ,
  • 王文博 ,
  • 原现军 ,
  • 普布卓玛 ,
  • 罗增 ,
  • 参木友
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  • 1. 省部共建青稞和牦牛种质资源与遗传改良国家重点实验室, 拉萨 850000;
    2. 西藏自治区农牧科学院草业科学研究所, 拉萨 850000;
    3. 南京农业大学草业学院饲草调制加工与贮藏研究所, 南京 210095
鲍宇红(1984-),女,内蒙古赤峰人,副研究员,硕士,从事反刍动物营养与饲料科学研究。E-mail:baoke626@163.com

收稿日期: 2021-04-20

  网络出版日期: 2021-11-10

基金资助

西藏自治区科技厅重点研发项目西藏农作物秸秆综合利用技术集成与示范(XZ201901NB07);国家自然科学基金(31960355);国家肉牛牦牛产业体系拉萨综合试验站(CARS-37)

Comparative Study on Nutritive Value of Kobresia tibetica of Alpine Meadow in Dangxiong and Naqu regions of Qinghai-Tibet Plateau

  • BAO Yuhong ,
  • WANG Wenbo ,
  • YUAN Xianjun ,
  • Pubuzhuoma ,
  • LUO Zeng ,
  • CAN Muyou
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  • 1. State Key Laboratory of Germplasm Resources and Genetic Improvement of Tibetan Barley and Yak, Lhasa 850000, China;
    2. Institute of Grass Science, Tibet Academy of Agriculture and Animal Husbandry Sciences, Lhasa 850000, China;
    3. Institute of Ensiling and Processing of Grass, College of Prataculture Science, Nanjing Agricultural University, Nanjing 210095, China

Received date: 2021-04-20

  Online published: 2021-11-10

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摘要

本研究旨在比较青藏高原那曲和当雄区域高山草甸藏嵩草营养价值。采集了8月和11月生长于当雄(海拔4 200 m)和那曲区域(海拔4 450 m)高山草甸的藏嵩草,对其进行营养成分和体外消化率分析。结果表明:1)2个区域11月藏嵩草粗脂肪(EE)含量显著低于8月(P<0.05)。当雄区域藏嵩草非结构性碳水化合物(CNSC)和糖类(CA)比例显著低于那曲区域(P<0.05),且2个区域8月藏嵩草CNSC和CA比例显著高于11月(P<0.05)。2个区域8月藏嵩草粗蛋白质(CP)比例均显著高于11月(P<0.05)。2个区域8月藏嵩草非蛋白氮(PA)比例均显著高于11月(P<0.05),且那曲区域藏嵩草PA比例显著高于当雄区域(P<0.05)。2)2个区域8月藏嵩草体外有机物消化率(IVOMD)无显著差异(P>0.05),当雄区域11月藏嵩草IVOMD显著低于那曲区域(P<0.05),当雄区域11月藏嵩草IVOMD显著低于8月(P<0.05)。2个区域8月藏嵩草代谢能(ME)无显著差异(P>0.05),2个区域11月藏嵩草ME均显著低于8月(P<0.05),且那曲区域11月藏嵩草ME显著高于当雄区域(P<0.05)。2个区域8月藏嵩草瘤胃液氨态氮/总氮(NH3-N/TN)均显著低于11月(P<0.05)。2个区域8月藏嵩草瘤胃液乙酸含量无显著差异(P>0.05),11月藏嵩草瘤胃液乙酸含量均显著低于8月(P<0.05);那曲区域11月藏嵩草瘤胃液乙酸含量显著高于当雄区域(P<0.05)。2个区域8月藏嵩草瘤胃液丙酸含量无显著差异(P>0.05),当雄区域11月藏嵩草瘤胃液丙酸含量显著低于8月(P<0.05);那曲区域11月藏嵩草瘤胃液丙酸含量显著高于当雄区域(P<0.05)。综上所述,生长区域和时间影响了藏嵩草的营养价值和体外消化率,8月藏嵩草CNSC、CA比例以及IVDMD、IVOMD和ME均高于11月,那曲区域藏嵩草CNSC和PA比例始终高于当雄区域。

本文引用格式

鲍宇红 , 王文博 , 原现军 , 普布卓玛 , 罗增 , 参木友 . 青藏高原那曲和当雄区域高山草甸藏嵩草营养价值比较研究[J]. 动物营养学报, 2021 , 33(11) : 6233 -6244 . DOI: 10.3969/j.issn.1006-267x.2021.11.024

Abstract

This study was conducted to compare the nutritive value of Kobresia tibetica of alpine meadow in Dangxiong and Naqu regions of Qinghai-Tibet Plateau. The Kobresia tibetans grown in alpine meadow of Dangxiong (4 200 m above sea level) and Naqu regions (4 450 m above sea level) in August and November were collected, then the nutrient composition and in vitro digestibility of Kobresia tibetans were analyzed. The results showed as follows:1) the content of ether extract (EE) of Kobresia tibetica in two regions in November was significantly lower than that in August (P<0.05). The proportions of non-structural carbohydrates (CNSC) and sugars (CA) of Kobresia tibetica in Dangxiong region were significantly lower than those in Naqu region (P<0.05), and the proportions of CNSC and CA of Kobresia tibetica in two regions in August was significantly higher than that in November (P<0.05). The content of crude protein (CP) of Kobresia tibetica in two regions in August was significantly higher than that in November (P<0.05). The proportion of non-protein nitrogen (PA) of Kobresia tibetica in two regions in August was significantly higher than that in November (P<0.05), and the proportion of PA of Kobresia tibetica in Naqu region was significantly higher than that in Dangxiong region (P<0.05). 2) There was no significant difference in the in vitro organic matter digestibility (IVOMD) of Kobresia tibetica between two areas in August (P>0.05), the IVOMD of Kobresia tibetica in Dangxiong region in November was significantly lower than that in Naqu region (P<0.05), and the IVOMD of Kobresia tibetica in Dangxiong region in November was significantly lower than that in August (P<0.05). There was no significant difference in the metabolizable energy (ME) of Kobresia tibetica between two regions in August (P>0.05), the ME of Kobresia tibetica in two regions in November was significantly lower than that in August (P<0.05), and the ME of Kobresia tibetica in Naqu region in November was significantly higher than that in Dangxiong region (P<0.05). The rumen fluid ammonia nitrogen/total nitrogen (NH3-N/TN) of Kobresia tibetica in two regions in August was significantly lower than that in November (P<0.05). There was no significant difference in the rumen fluid acetic acid content of Kobresia tibetica between two areas in August (P>0.05), and the rumen fluid acetic acid content of Kobresia tibetica in November was significantly lower than that in August (P<0.05); the rumen fluid acetic acid content of Kobresia tibetica in Naqu region in November was significantly higher than that in Dangxiong region (P<0.05). There was no significant difference in the rumen fluid propionic acid content of Kobresia tibetica between two regions in August (P>0.05), and the rumen fluid propionic acid content of Kobresia tibetica in November was significantly lower than that in August (P<0.05); the rumen fluid propionic acid content of Kobresia tibetica in Naqu region in November was significantly higher than that in Dangxiong region (P<0.05). In conclusion, the growth region and time can affect the nutritive value and in vitro digestibility of Kobresia Tibetans, the CNSC and CA proportions and IVDMD, IVOMD and ME of Kobresia tibetica in August are higher than those in November, and the proportions of CNSC and PA of Kobresia tibetica in Naqu region are always higher than those in Dangxiong region.

参考文献

[1] 周兴民, 王质彬, 杜庆.青海植被[M].西宁:青海人民出版社, 1987:87-89. ZHOU X M, WANG Z B, DU Q.Vegetation of Qinghai province[M].Xining:Qinghai People's Publishing House, 1987:87-89.(in Chinese)
[2] 李亮, 王利, 王鑫, 等.浅析海拔梯度对植物生长发育的影响[J].防护林科技, 2016, 152(5):67-68. LI L, WANG L, WANG X, et al.Analysis on the influence of altitude gradient on plant growth and development[J].Protection Forest Science and Technology, 2016, 152(5):67-68.(in Chinese)
[3] 朱玉环, 廉美娜, 郭旭生.藏嵩草绿汁发酵液提高苜蓿青贮发酵品质[J].农业工程学报, 2013, 29(5):199-206. ZHU Y H, LIAN M N, GUO X S.Improve fermentation quality of alfalfa silage by addition of fermented juice prepared from Kobresia littledalei[J].Transactions of the Chinese Society of Agricultural Engineering, 2013, 29(5):199-206.(in Chinese)
[4] 王力, 李凤霞, 周万福, 等.气候变化对不同海拔高山嵩草物候期的影响[J].草业科学, 2012, 29(8):1256-1261. WANG L, LI F X, ZHOU W F, et al.Impacts of climate change on Kobresia pygmaea phenophase at different altitude regions in Qinghai Plateau[J].Pratacultural Science, 2012, 29(8):1256-1261.(in Chinese)
[5] 张丽英.饲料分析及饲料质量检测技术[M].4版.北京:中国农业大学出版社, 2016. ZHANG L Y.Feed analysis and quality test technology[M].4th ed.Beijing:China Agricultural University Press, 2016.(in Chinese)
[6] VAN SOEST P J, ROBERTSON J B, LEWIS B A.Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition[J].Journal of Dairy Science, 1991, 74(10):3583-3597.  
[7] SNIFFEN C J, O'CONNOR J D, VAN SOEST P J, et al.A net carbohydrate and protein system for evaluating cattle diets:Ⅱ.Carbohydrate and protein availability[J].Journal of Animal Science, 1992, 70(11):3562-3577.  
[8] MENKE H H, STEINGASS H.Estimation of the energetic feed value obtained from chemical analysis and in vitro gas production using rumen fluid[J].Animal Research and Development, 1988, 28:7-55.
[9] LI X Z, LONG R J, YAN C G, et al.Rumen microbial responses in fermentation characteristics and production of CLA and methane to linoleic acid in associated with malate or fumarate[J].Animal Feed Science and Technology, 2010, 155(2/3/4):132-139.
[10] 巴桑珠扎, 陈亮, 奥斯曼.西藏地区不同作物秸秆体外发酵特性研究[J].动物营养学报, 2017, 29(2):719-728. BA S Z Z, CHEN L, AO S M.Study on in vitro fermentation characteristics of different crop straws in Tibet region[J].Chinese Journal of Animal Nutrition, 2017, 29(2):719-728.(in Chinese)
[11] 张春林, 韩向敏, 郎侠, 等.青藏高原东北缘不同草地类型牧草营养品质动态[J].草业科学, 2019, 36(3):763-771. ZHANG C L, HAN X M, LANG X, et al.Forage nutrition from different grassland types at different growth stages on the northeast margin of the Qinghai-Tibet Plateau[J].Pratacultural Science, 2019, 36(3):763-771.(in Chinese)
[12] 席冬梅, 陈勇, 彭洪清.多花黑麦草不同生长期营养价值评定[J].草原与草坪, 2005(2):62-64. XI D M, CHEN Y, PENG H Q.The evalution of nutritive value of perennial ryegrass[J].Grassland and Turf, 2005(2):62-64.(in Chinese)
[13] HAN F, BEN G Y, SHI S B.Contents of protein, fat and starch of Kobresia humilis plants grown at different altitudes in Qinghai-Xizang plateau[J].Acta Phytoecological Sinica, 1997, 21(2):97-104.
[14] SINGH B, TODARIA N P.Nutrients composition changes in leaves of Quercus semecarpifolia at different seasons and altitudes[J].Annals of Forest Research, 2012, 55(2):189-196.
[15] KAMALAK A, CANBOLAT O, GURBUZ Y, et al.Effect of maturity stage on chemical composition, in vitro and in situ dry matter degradation of tumbleweed hay (Gundelia tournefortii L.)[J].Small Ruminant Research, 2005, 58(2):149-156.  
[16] 赵忠, 王安禄, 马海生, 等.青藏高原东缘草地生态系统动态定位监测与可持续发展要素研究Ⅱ.高寒草甸草地生态系统植物群落结构特征及物种多样性分析[J].草业科学, 2002, 19(6):9-13. ZHAO Z, WANG A L, MA H S, et al.Studies on dynamics monitor and sustainable development in eastern edge of Qinghai-Tibetan alpine grassland Ⅱ.Analysis of plant community structural features and grass species diversity[J].Pratacultural Science, 2002, 19(6):9-13.(in Chinese)
[17] SHAH M K, TAMANG B B, DHAKAL B, et al.Nutritive values of fodders at different seasons and altitudes in Gandaki River Basin of Nepal[J].Journal of Agriculture and Natural Resources, 2019, 2(1):109-126.  
[18] SKAPETAS B, NITAS D, KARALAZOS A, et al.A study on the herbage mass production and quality for organic grazing sheep in a mountain pasture of northern Greece[J].Livestock Production Science, 2004, 87(2/3):277-281.
[19] MOREIRA F B, PRADO I N, CECATO U, et al.Forage evaluation, chemical composition, and in vitro digestibility of continuously grazed star grass[J].Animal Feed Science and Technology, 2004, 113(1/2/3/4):239-249.
[20] MOONEY H A, BILLINGS W D.Effects of altitude on carbohydrate content of mountain plants[J].Ecology, 1965, 46(5):750-751.  
[21] 林德辉, 李存信.不同海拔地区种植的水稻次库碳水化合物含量的比较[J].植物分类与资源学报, 1988(1):87-92. LIN D H, LI C X.Comparison of carbohydrate contents in secondary sink (stem+leaf sheath) of rice grown at different altitude locaities[J].Plant Diversity and Resources, 1988(1):87-92.(in Chinese)
[22] SHI P L, KÖRNER C, HOCH G.End of season carbon supply status of woody species near the treeline in western China[J].Basic and Applied Ecology, 2006, 7(4):370-377.  
[23] 徐雅梅, 苗彦军, 呼天明, 等.藏北嵩草种质资源评价[J].中国草地学报, 2009, 31(3):41-46. XU Y M, MIAO Y J, HU T M, et al.Evaluation of germplasm resources of Kobresia littledalei in Tibet[J].Chinese Journal of Grassland, 2009, 31(3):41-46.(in Chinese)
[24] SARWAR M, KHAN M U N, SAEED M N.Influence of nitrogen fertilization and stage of maturity of mottgrass (Pennisetum purpureum) on its composition, dry matter intake, ruminal characteristics and digestion kinetics in cannulated buffalo bulls[J].Animal Feed Science and Technology, 1999, 82(1/2):121-130.
[25] Dairy Cattle Nutrition, Committee on Animal Nutrition, Board on Agriculture and Natural Resources, et al.Nutrient requirements of dairy cattle[M].7th ed.Washington, D.C.:National Academies Press, 2001.
[26] 龚明, 刘友良, 朱培仁.低温下稻苗叶片中蛋白质及游离氨基酸的变化[J].植物生理学通讯, 1989(4):18-22. GONG M, LIU Y L, ZHU P R.Changes of proteins and free amino acids in leaves of rice seedlings during chilling stress[J].Plant Physiology Communications, 1989(4):18-22.(in Chinese)
[27] ELIZALDE J C, MERCHEN N R, FAULKNER D B.Fractionation of fiber and crude protein in fresh forages during the spring growth[J].Journal of Animal Science, 1999, 77(2):476-484.  
[28] ALZUETA C, CABALLERO R, REBOLÉ A, et al.Crude protein fractions in common vetch (Vicia sativa L.) fresh forage during pod filling[J].Journal of Animal Science, 2001, 79(9):2449-2455.  
[29] 云颖, 赵苗苗, 双胡尔, 等.刈割期和添加剂对苜蓿青贮发酵品质和CNCPS蛋白组分的影响[J].草业科学, 2017, 34(10):2149-2156. YUN Y, ZHAO M M, SHUANG H E, et al.Effect of mowing period and additives on fermentation quality and CNCPS protein components of alfalfa silage[J].Pratacultural Science, 2017, 34(10):2149-2156.(in Chinese)
[30] BLVMMEL M, ØRSKOV E R.Comparison of in vitro gas production and nylon bag degradability of roughages in predicting feed intake in cattle[J].Animal Feed Science and Technology, 1993, 40(2/3):109-119.
[31] BAL M A, OZTURK D, AYDIN R, et al.Nutritive value of sainfoin (Onobrychis viciaefolia) harvested at different maturity stages[J].Pakistan Journal of Biological Sciences, 2006, 9:205-209.
[32] KAMALAK A, ATALAY A, ÖZKAN Ç Ö, et al.Determination of potential nutritive value of Trigonella kotschi fenzl hay harvested at three different maturity stages[J].Kafkas Vniversitesi Veteriner Fakültesi Dergisi, 2011, 17(4):635-640.  
[33] 徐世晓, 赵新全, 孙平, 等.青藏高原不同海拔高度对禾本科牧草体外消化率的影响[J].草地学报, 2002, 10(2):124-127. XU S X, ZHAO X Q, SUN P, et al.Altitude impact on vitro digestibility of gramineous herbage grown in Tibetan Plateau[J].Acta Agrestia Sinica, 2002, 10(2):124-127.(in Chinese)
[34] EMMANUEL R, MUTIMURA M.Comparison of nutrient composition and in vitro digestion characteristics of four forage legumes from two agro-ecological zones of Rwanda[J].Agricultural Journal, 2012, 7(5):354-359.  
[35] 余苗, 钟荣珍, 周道玮, 等.不同生育期虎尾草的体外发酵产气特性[J].草业科学, 2014, 31(5):956-964. YU M, ZHONG R Z, ZHOU D W, et al.Research on in vitro fermentation characteristics of Chloris virgata at different growth stages[J].Pratacultural Science, 2014, 31(5):956-964.(in Chinese)
[36] MAO H L, WANG J K, ZHOU Y Y, et al.Effects of addition of tea saponins and soybean oil on methane production, fermentation and microbial population in the rumen of growing lambs[J].Livestock Science, 2010, 129(1/2/3):56-62.
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