反刍与草食动物营养与饲料 RUMINANT AND HERBIVORE NUTRITION AND FEED

不同发酵乳酸菌剂及组合添加对甜高粱青贮营养价值、微生物数量及有氧稳定性的影响

  • 付薇 ,
  • 陈伟 ,
  • 王小利 ,
  • 孟军江 ,
  • 覃涛英 ,
  • 韩永芬
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  • 1. 贵州省草业研究所, 贵阳 550006;
    2. 贵州金农富平生态农牧科技有限公司, 松桃 554100
付薇(1985-),女,四川广汉人,副研究员,硕士,研究方向为饲草资源开发与利用。E-mail:494978600@qq.com

收稿日期: 2021-04-11

  网络出版日期: 2021-11-10

基金资助

贵州省科技创新人才团队项目(20205005);贵州省科技计划项目(2021179)

Effects of Different Lactic Acid Bacteria Agents and Their Combinations on Nutritional Value, Microorganisms Number and Aerobic Stability of Sweet Sorghum Silage

  • FU Wei ,
  • CHEN Wei ,
  • WANG Xiaoli ,
  • MENG Junjiang ,
  • QIN Taoying ,
  • HAN Yongfen
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  • 1. Guizhou Institute of Prataculture, Guiyang 550006, China;
    2. Guizhou Jinnongfuping Sci&Tech Co., Ltd. of Ecological Agriculture and Animal Husbandry, Songtao 554100, China

Received date: 2021-04-11

  Online published: 2021-11-10

Supported by

 

摘要

本试验旨在研究不同发酵乳酸菌剂及组合添加对甜高粱青贮营养价值、微生物数量及有氧稳定性的影响,为甜高粱青贮复合菌剂的研制提供科技支撑。根据生长活性和产酸速率测定分析,试验筛选得到活性较强、产酸速率较高的2株短乳杆菌LBR02、LBR07和1株发酵乳杆菌LFE19。将前期获得的植物乳杆菌LP06和戊糖片球菌PP02与上述3株菌株制成发酵菌剂,发酵菌剂添加量均为5×105 CFU/g,分为8个处理:1) CK处理(无乳酸菌);2) T处理(植物乳杆菌LP06 :戊糖片球菌PP02=1 : 1);3) Y1处理(短乳杆菌LBR02);4) Y2处理(短乳杆菌LBR07);5) Y3处理(发酵乳杆菌LFE19);6) T+Y1处理(植物乳杆菌LP06 :戊糖片球菌PP02 :短乳杆菌LBR02=1 : 1 : 2);7) T+Y2处理(植物乳杆菌LP06 :戊糖片球菌PP02 :短乳杆菌LBR07=1 : 1 : 2);8) T+Y3处理(植物乳杆菌LP06 :戊糖片球菌PP02 :发酵乳杆菌LFE19=1 : 1 : 2)。对8个处理青贮60 d和有氧暴露后第0、3、6、9天的pH、营养价值、微生物数量及有氧稳定性进行分析。结果表明:1)青贮60 d时,各处理pH差异不显著(P>0.05),均低于4.1。T处理粗蛋白质含量显著高于其他处理(P<0.05),CK、Y3、T+Y3处理可溶性碳水化合物含量显著高于其他处理(P<0.05)。各处理干物质、中性洗涤纤维和酸性洗涤纤维含量均差异不显著(P>0.05)。与青贮前相比,青贮60 d时,各处理乳酸菌数量显著提高(P<0.05),酵母菌和霉菌数量显著降低(P<0.05)。2)有氧暴露第9天,Y1、Y2、T+Y1、T+Y2处理pH和酵母菌、霉菌数量显著低于其他处理(P<0.05),干物质、可溶性碳水化合物含量和有氧稳定性显著高于其他处理(P<0.05)。3)根据隶属函数法综合评价,各处理综合价值从高到低依次为:T+Y1处理>Y1处理>T+Y2处理>Y2处理>Y3处理>T处理>T+Y3处理>CK。综上分析,不同发酵乳酸菌剂及组合添加均可不同程度改善甜高粱青贮发酵品质,有氧暴露后,短乳杆菌LBR02单独添加或与植物乳杆菌LP06、戊糖片球菌PP02组合添加在保存甜高粱营养价值、有效抑制酵母菌和霉菌生长、提高青贮有氧稳定性方面作用效果更好。

本文引用格式

付薇 , 陈伟 , 王小利 , 孟军江 , 覃涛英 , 韩永芬 . 不同发酵乳酸菌剂及组合添加对甜高粱青贮营养价值、微生物数量及有氧稳定性的影响[J]. 动物营养学报, 2021 , 33(11) : 6245 -6256 . DOI: 10.3969/j.issn.1006-267x.2021.11.025

Abstract

This experiment was conducted to study the effects of different lactic acid bacteria agents and their combinations on nutritional value, microorganisms number and aerobic stability of sweet sorghum silage, and to provide scientific support for the development of sweet sorghum silage composite additives. According to the determination and analysis of growth activity and acid production rate, two strains of Lactobacillus brevis LBR02, LBR07 and one strain of Lactobacillus fermentum LFE19 with strong activity and high acid production rate were screened. Lactobacillus plantarum LP06 and Pediococcus pentosus PP02 which obtained in earlier stage were mixed with the above three strains to make fermentation agent, the amount of fermentation agent was all 5×105 CFU/g, eight treatments were designed:1) CK treatment (no lactic acid bacteria); 2) T treatment (Lactobacillus plantarum LP06:Pediococcus pentosus PP02=1:1); 3) Y1 treatment (Lactobacillus brevis LBR02);4) Y2 treatment (Lactobacillus brevis LBR07); 5) Y3 treatment (Lactobacillus fermentum LFE19); 6) T+Y1 treatment (Lactobacillus plantarum LP06:Pediococcus pentosus PP02:Lactobacillus brevis LBR02=1:1:2); 7) T+Y2 treatment (Lactobacillus plantarum LP06:Pediococcus pentosus PP02:Lactobacillus brevis LBR07=1:1:2); 8) T+Y3 treatment (Lactobacillus plantarum LP06:Pediococcus pentosus PP02:Lactobacillus fermentum LFE19=1:1:2). The pH, nutritional value, microorganisms number and aerobic stability of 8 silage treatments were analyzed on 60 days fermentation and days 0, 3, 6, 9 of aerobic exposure. The results showed as follows:1) at 60 days of silage, there was no significant difference in the pH of all treatments (P>0.05), which was lower than 4.1. The crude protein content of T treatment was significantly higher than that of other treatments (P<0.05), the soluble carbohydrate content of CK, Y3, T+Y3 treatment was significantly higher than that of other treatments (P<0.05). There were no significant differences in dry matter, neutral detergent fiber and acid detergent fiber contents among all treatments (P>0.05). Compared with before silage, at 60 days of silage, the lactic acid bacteria number of all treatments was significantly increased (P<0.05), and the yeast and mould numbers were significantly decreased (P<0.05). 2) On day 9 of aerobic exposure, the pH and yeast and mould numbers of Y1, Y2, T+Y1 and T+Y2 treatments were significantly lower than those of other treatments (P<0.05), and the dry matter, soluble carbohydrate contents and aerobic stability were significantly higher than those of other treatments (P<0.05). 3) According to the comprehensive evaluation of membership function method, the comprehensive value of each treatment from high to low was:T+Y1 treatment>Y1 treatment>T+Y2 treatment>Y2 treatment>Y3 treatment>T treatment>T+Y3 treatment>CK treatment. In conclusion, adding different lactic acid bacteria agents and their combinations can improve the fermentation quality of sweet sorghum silage to varying degrees. After aerobic exposure, Lactobacillus brevis LBR02 added alone or combined with Lactobacillus plantarum LP06 and Pediococcus pentosus PP02 have better effects on preserving the nutritional value of silage, effectively inhibiting the growth of yeast and mold and improving the aerobic stability of silage.

参考文献

[1] ZHANG S J, CHAUDHRY A S, RAMDANI D, et al.Chemical composition and in vitro fermentation characteristics of high sugar forage sorghum as an alternative to forage maize for silage making in Tarim Basin, China[J].Journal of Integrative Agriculture, 2016, 15(1):175-182.  
[2] AMER S, SEGUIN P, MUSTAFA A F.Short communication:effects of feeding sweet sorghum silage on milk production of lactating dairy cows[J].Journal of Dairy Science, 2012, 95(2):859-863.  
[3] GUO J G, XIE Y X, YU Z, et al.Effect of Lactobacillus plantarum expressing multifunctional glycoside hydrolases on the characteristics of alfalfa silage[J].Applied Microbiology and Biotechnology, 2019, 103(19):7983-7995.  
[4] 黄峰, 张露, 周波, 等.青贮微生物及其对青贮饲料有氧稳定性影响的研究进展[J].动物营养学报, 2019, 31(1):82-89. HUANG F, ZHANG L, ZHOU B, et al.Research process in silage microorganism and its effect on silage aerobic stability[J].Chinese Journal of Animal Nutrition, 2019, 31(1):82-89.(in Chinese)
[5] KOC F, AKSOY S O, OKUR A A, et al.Effect of pre-fermented juice, Lactobacillus plantarum and Lactobacillus buchneri on the fermentation characteristics and aerobic stability of high dry matter alfalfa bale silage[J].The Journal of Animal & Plant Sciences, 2017, 27(5):1426-1431.
[6] LIU Q H, SHAO T, BAI Y F.The effect of fibrolytic enzyme;Lactobacillus plantarum, and two food antioxidants on the fermentation quality, alpha-tocopherol and beta-carotene of high moisture napier grass silage ensiled at different temperatures[J].Animal Feed Science and Technology, 2016, 221(Part A):1-11.
[7] 贾婷婷, 吴哲, 玉柱.不同类型乳酸菌添加剂对燕麦青贮品质和有氧稳定性的影响[J].草业科学, 2018, 35(5):1266-1272. JIA T T, WU Z, YU Z.Effect of different lactic acid bacteria additives on the fermentation quality and aerobic stability of oat silage[J].Pratacultural Science, 2018, 35(5):1266-1272.(in Chinese)
[8] POLUKIS S A, SMITH M L, SAVAGE R M, et al.The effect of two microbial inoculants on the aerobic stability of high-moisture corn[J].Journal of Animal Science, 2016, 94(Suppl 5):324.
[9] RABELO C H S, BASSO F C, MCALLISTER T A, et al.Influence of Lactobacillus buchneri as silage additive and forage:concentrate ratio on the growth performance, fatty acid profile in longissimus muscle, and meat quality of beef cattle[J].Canadian Journal of Animal Science, 2016, 96(4):550-562.  
[10] 塔娜, 魏日华, 德庆哈拉, 等.对禾草源同型发酵和/或异型发酵乳酸菌发酵无芒雀麦青贮有氧稳定性的评价[J].动物营养学报, 2017, 29(4):1301-1311. TA N, WEI R H, DE Q H L, et al.Evaluation of aerobic stability of Bromus inermis Leyss. silage fermented by homofermentative and/or heterofermentative lactic acid bacteria from grass[J].Chinese Journal of Animal Nutrition, 2017, 29(4):1301-1311.(in Chinese)
[11] 李菲菲, 张凡凡, 王旭哲, 等.同/异型发酵乳酸菌对全株玉米青贮营养成分和瘤胃降解特征的影响[J].草业学报, 2019, 28(6):128-136. LI F F, ZHANG F F, WANG X Z, et al.Effects of homo-and heterofermentative lactic acid bacteria on the nutritional quality and ruminal degradation rate of the whole plant maize silage[J].Acta Prataculturae Sinica, 2019, 28(6):128-136.(in Chinese)
[12] 王旭哲, 张凡凡, 马春晖, 等.同/异型乳酸菌对青贮玉米开窖后品质及微生物的影响[J].农业工程学报, 2018, 34(10):296-304. WANG X Z, ZHANG F F, MA C H, et al.Corn silage fermentation quality and microbial populations as influenced by adding homo- and hetero-fermentative bacteria after silos opened[J].Transactions of the Chinese Society of Agricultural Engineering, 2018, 34(10):296-304.(in Chinese)
[13] FERRERO F, TABACCO E, PIANO S, et al.Temperature during conservation in laboratory silos affects fermentation profile and aerobic stability of corn silage treated with Lactobacillus buchneri, Lactobacillus hilgardii, and their combination[J].Journal of Dairy Science, 2021, 104(2):1696-1713.  
[14] 孙茜, 徐圣君, 曾贤桂, 等.动物青贮饲料添加剂的研究进展[J].中国农学通报, 2020, 36(27):158-164. SUN Q, XU S J, ZENG X G, et al.Animal silage additives:research progress[J].Chinese Agricultural Science Bulletin, 2020, 36(27):158-164.(in Chinese)
[15] NAIR J, HUAXIN N, ANDRADA E, et al.Effects of inoculation of corn silage with Lactobacillus hilgardii and Lactobacillus buchneri on silage quality, aerobic stability, nutrient digestibility, and growth performance of growing beef cattle[J].Journal of Animal Science, 2020, 98(10):skaa267.
[16] 罗撄宁, 罗盈, 包锦泽, 等.添加乳酸菌和香草醛对甜玉米籽实青贮饲料品质的影响[J].饲料工业, 2020, 41(19):50-53. LUO Y N, LUO Y, BAO J Z, et al.Effects of adding lactic acid bacteria and vanillin on silage quality of corn seed[J].Feed Industry, 2020, 41(19):50-53.(in Chinese)
[17] 徐生阳, 闵旭东, 云颖, 等.短乳杆菌对有氧暴露过程中不同品种全株玉米青贮品质的影响[J].草业科学, 2019, 36(1):252-260. XU S Y, MIN X D, YUN Y, et al.Effect of Lactobacillus brevis on the quality of whole plant corn silages during aerobic exposure[J].Pratacultural Science, 2019, 36(1):252-260.(in Chinese)
[18] AOAC.Official methods of analysis of AOAC[S].18th ed.Arlington:Association of Officiating Analytical Chemists, 2005.
[19] DUBOIS M, GILLES K A, HAMILTON J K, et al.Colorimetric method for determination of sugars and related substances[J].Analytical Chemistry, 1956, 28(3):350-356.  
[20] VAN SOEST P J, ROBERTSON J B, LEWIS B A.Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition[J].Journal of Dairy Science, 1991, 74(10):3583-3597.  
[21] 李小铃, 关皓, 帅杨, 等.单一和复合乳酸菌添加剂对扁穗牛鞭草青贮品质的影响[J].草业学报, 2019, 28(6):119-127. LI X L, GUAN H, SHUAI Y, et al.Effects of single and multiple inoculants on Hemarthria compressa silage quality[J].Acta Prataculturae Sinica, 2019, 28(6):119-127.(in Chinese)
[22] 苗芳, 张凡凡, 唐开婷, 等.同/异质型乳酸菌添加对全株玉米青贮发酵特性、营养品质及有氧稳定性的影响[J].草业学报, 2017, 26(9):167-175. MIAO F, ZHANG F F, TANG K T, et al.Effects of homo-and hetero-fermentative lactic acid bacteria on the fermentation characteristics, nutritional quality, and aerobic stability of whole corn silage[J].Acta Prataculturae Sinica, 2017, 26(9):167-175.(in Chinese)
[23] 魏日华.禾本科牧草异型发酵乳酸菌的分离鉴定及对青贮发酵品质和有氧稳定性的影响[D].硕士学位论文.呼和浩特:内蒙古农业大学, 2010. WEI R H.Isolation and screening of heterofermentative lactic bacteria from Gramineas and the effect of adding it on silage fermentation quality and aerobic stability[D].Master's Thesis.Hohhot:Inner Mongolia Agricultural University, 2010.(in Chinese)
[24] ZHANG F F, WANG X Z, LU W H, et al.Meta-analysis of the effects of combined homo- and heterofermentative lactic acid bacteria on the fermentation and aerobic stability of corn silage[J].International Journal of Agriculture and Biology, 2018, 20(8):1846-1852.
[25] OLIVEIRA A S, WEINBERG Z G, OGUNADE I M, et al.Meta-analysis of effects of inoculation with homofermentative and facultative heterofermentative lactic acid bacteria on silage fermentation, aerobic stability, and the performance of dairy cows[J].Journal of Dairy Science, 2017, 100(6):4587-4603.  
[26] 韩立英, 玉柱, 周禾.乳酸菌和纤维素酶对直穗鹅观草青贮的改善效果[J].草业科学, 2013, 30(9):1439-1444. HAN L Y, YU Z, ZHOU H.Effects of lactic acid bacteria inoculants and enzymes on Roegneria turczaninovii silages[J].Pratacultural Science, 2013, 30(9):1439-1444.(in Chinese)
[27] JACOBS J L, MCKENZIE F R, RIGBY S E, et al.Effect of nitrogen fertiliser application and length of lock up on dairy pasture dry matter yield and quality for silage in south-western Victoria[J].Australian Journal of Experimental Agriculture, 1998, 38(3):219-226.  
[28] 代胜, 王飞, 董祥, 等.紫花苜蓿与甜高粱混合比例对发酵全混合日粮营养品质及有氧稳定性的影响[J].动物营养学报, 2020, 32(5):2306-2315. DAI S, WANG F, DONG X, et al.Effects of mixing ratio of alfalfa and sweet sorghum on nutritional quality and aerobic stability of total mixed ration silage[J].Chinese Journal of Animal Nutrition, 2020, 32(5):2306-2315.(in Chinese)
[29] 刘晗璐.禾本科牧草乳酸菌发现及发酵品质检测与动物生产性能影响研究[D].博士学位论文.呼和浩特:内蒙古农业大学, 2008. LIU H L.The effect of epiphytic lactic acid bacteria on gramineous grass silage fermentation characteristics and dairy cows performance[D].Ph.D.Thesis.Hohhot:Inner Mongolia Agricultural University, 2008.(in Chinese)
[30] FILYA I.The effect of Lactobacillus buchneri and Lactobacillus plantarum on the fermentation, aerobic stability, and ruminal degradability of low dry matter corn and sorghum silages[J].Journal of Dairy Science, 2003, 86(11):3575-3581.  
[31] WANG M S, YANG C H, JIA L J, et al.Effect of Lactobacillus buchneri and Lactobacillus plantarum on the fermentation characteristics and aerobic stability of whipgrass silage in laboratory silos[J].Grassland Science, 2014, 60(4):233-239.  
[32] DRIEHUIS F, OUDE ELFERINK S J W H, VAN WIKSELAAR P G.Fermentation characteristics and aerobic stability of grass silage inoculated with Lactobacillus buchneri, with or without homofermentative lactic acid bacteria[J].Grass and Forage Science, 2001, 56(4):330-343.  
[33] HUISDEN C M, ADESOGAN A T, KIM S C, et al.Effect of applying molasses or inoculants containing homofermentative or heterofermentative bacteria at two rates on the fermentation and aerobic stability of corn silage[J].Journal of Dairy Science, 2009, 92(2):690-697.  
[34] DRIEHUIS F, ELFERINK S J, SPOELSTRA S F.Anaerobic lactic acid degradation during ensilage of whole crop maize inoculated with Lactobacillus buchneri inhibits yeast growth and improves aerobic stability[J].Journal of Applied Microbiology, 1999, 87(4):583-594.  
[35] 王亚芳, 姜富贵, 成海建, 等.不同青贮添加剂对全株玉米青贮营养价值、发酵品质和瘤胃降解率的影响[J].动物营养学报, 2020, 32(6):2765-2774. WANG Y F, JIANG F G, CHENG H J, et al.Effects of different silage additives on nutritional value, fermentation quality and rumen degradability of whole corn silage[J].Chinese Journal of Animal Nutrition, 2020, 32(6):2765-2774.(in Chinese)
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