反刍与草食动物营养与饲料 RUMINANT AND HERBIVORE NUTRITION AND FEED

不同剩余采食量下滩羊能量代谢分析

  • 张倩 ,
  • 王俊奎 ,
  • 罗芳 ,
  • 和东迁 ,
  • 陈丽尧 ,
  • 卢童童 ,
  • 吴少飞 ,
  • 张瑞雪 ,
  • 陶金忠
展开
  • 宁夏大学农学院, 银川 750021
张倩(1997-),女,宁夏吴忠人,硕士研究生,从事动物分子育种研究。E-mail:1136384402@qq.com

收稿日期: 2021-04-21

  网络出版日期: 2021-11-10

基金资助

宁夏重点研发一般项目(2019BEH03004)

Energy Metabolism Analysis of Tan Sheep with Different Residual Feed Intake

  • ZHANG Qian ,
  • WANG Junkui ,
  • LUO Fang ,
  • HE Dongqian ,
  • CHEN Liyao ,
  • LU Tongtong ,
  • WU Shaofei ,
  • ZHANG Ruixue ,
  • TAO Jinzhong
Expand
  • College of Agriculture, Ningxia University, Yinchuan 750021, China

Received date: 2021-04-21

  Online published: 2021-11-10

Supported by

 

摘要

本研究旨在揭示不同剩余采食量(RFI)下滩羊血浆内源性能量代谢物的变化,筛选出对滩羊RFI有影响作用的差异代谢物。筛选极高、极低RFI滩羊各16只并采集血液,应用代谢组学技术对血浆样品进行检测,结合主成分分析(PCA)和正交偏最小二乘判别分析(OPLS-DA),以变异倍数(FC)>1.30或<0.77,变量投影重要度(VIP)>1和P<0.05为标准筛选差异代谢物。结果显示:极低RFI公羊vs极高RFI公羊组中D-木酮糖-5-磷酸、L-鸟氨酸盐酸盐下调;极低RFI母羊vs极高RFI母羊组中腺嘌呤、柠檬酸、L-天冬氨酸上调,L-瓜氨酸下调;极低RFI羊vs极高RFI羊组中腺嘌呤、柠檬酸上调,L-苏氨酸、D-木酮糖-5-磷酸下调。经受试者工作特征(ROC)曲线分析发现极低RFI母羊vs极高RFI母羊组中柠檬酸的ROC曲线下面积(AUC)>0.9。综上可知,性别会导致滩羊能量代谢不同,柠檬酸有望成为影响母滩羊RFI的候选生物标志物。

本文引用格式

张倩 , 王俊奎 , 罗芳 , 和东迁 , 陈丽尧 , 卢童童 , 吴少飞 , 张瑞雪 , 陶金忠 . 不同剩余采食量下滩羊能量代谢分析[J]. 动物营养学报, 2021 , 33(11) : 6309 -6319 . DOI: 10.3969/j.issn.1006-267x.2021.11.031

Abstract

The aim of this study was to reveal the changes of endogenous energy metabolites in plasma of Tan sheep under different residual feed intake (RFI), and to screen out the differential metabolites that have an effect on RFI of Tan sheep. Blood samples were collected from 16 Tan sheep with very high RFI and very low RFI, respectively, metabolomics was used to detect plasma samples. Combined with principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA), differential metabolites were screened according to the criteria of multiple of variation (FC)>1.3 or <0.77, variable projection importance (VIP)>1 and P<0.05. The results showed that D-xylose-5-phosphate and L-ornithine hydrochloride were down regulated in very low RFI rams vs very high RFI rams group; adenine, citric acid and L-aspartate were up-regulated and L-citrulline was down regulated in very low RFI ewes vs very high RFI ewes group; adenine and citric acid were up-regulated and L-threonine and D-xylose-5-phosphate were down regulated in very low RFI sheep vs very high RFI sheep group. The receiver operating characteristic (ROC) curve analysis showed that the area under the ROC curve (AUC) of citric acid in very low RFI vs very high RFI ewe group was more than 0.9. In conclusion, gender can lead to different energy metabolism of Tan sheep, and citric acid is expected to be a candidate biomarker for influencing RFI of female Tan sheep.

参考文献

[1] BASARAB J A, PRICE M A, AALHUS J L, et al.Residual feed intake and body composition in young growing cattle[J].Canadian Journal of Animal Science, 2003, 83(2):189-204.  
[2] 杨朝云.基于二代测序秦川牛剩余采食量相关RNA分子挖掘[D].硕士学位论文.银川:宁夏大学, 2019. YANG C Y.Screening of residual feeding intake related RNA molecular based on next-generation sequencing in Qinchuan Cattle[D].Master's Thesis.Yinchuan:Ningxia University, 2019.(in Chinese)
[3] PATIENCE J F.The influence of dietary energy on feed efficiency in grow-finish swine[M].Wageningen:Wageningen Academic Publishers, 2012:101-129.
[4] ARTHUR P F, ARCHER J A, JOHNSTON D J, et al.Genetic and phenotypic variance and covariance components for feed intake, feed efficiency, and other postweaning traits in Angus cattle[J].Journal of Animal Science, 2001, 79(11):2805-2811.  
[5] RICHARDSON E C, HERD R M, ODDY V H, et al.Body composition and implications for heat production of Angus steer progeny of parents selected for and against residual feed intake[J].Animal Production Science, 2001, 41(7):1065-1072.  
[6] ZHANG X X, WANG W M, MO F T, et al.Association of residual feed intake with growth and slaughtering performance, blood metabolism, and body composition in growing lambs[J].Scientific Reports, 2017, 7(1):12681.
[7] 谢云怡, 司敬方, 武轩宇, 等.不同剩余采食量水平的奶牛采食行为及体尺指标差异分析[J].畜牧与兽医, 2016, 48(8):58-61. XIE Y Y, SI J F, WU X Y, et al.Variation analysis on differences of feeding behaviors and body measurements in cows with different residual feed levels[J].Animal Husbandry & Veterinary Medicine, 2016, 48(8):58-61.(in Chinese)
[8] DUPONT J, SCARAMUZZI R J.Insulin signalling and glucose transport in the ovary and ovarian function during the ovarian cycle[J].Biochemical Journal, 2016, 473(11):1483-1501.  
[9] WALLIMANN T, TOKARSKA-SCHLATTNER M, SCHLATTNER U.The creatine kinase system and pleiotropic effects of creatine[J].Amino Acids, 2011, 40(5):1271-1296.  
[10] 罗芳, 郭延生, 马志远, 等.基于UPLC-Q-TOF MS代谢组学技术筛选表征西门塔尔种公牛精子活力的候选生物标志物[J].畜牧兽医学报, 2019, 50(8):1596-1606. LUO F, GUO Y S, MA Z Y, et al.Selection of candidate biomarkers featuring sperm motility in Simmental bull based on UPLC-Q-TOF MS metabolomics[J].Acta Veterinaria et Zootechnica Sinica, 2019, 50(8):1596-1606.(in Chinese)
[11] 江婧.基于代谢组学研究产前运动对围产期奶牛血浆代谢物影响[D].硕士学位论文.雅安:四川农业大学, 2019. JIANG J.Effects of prepartum exercise on plasma metabolites of the transition dairy cow-based on metabolomics[D].Master's thesis.Ya'an:Sichuan Agricultural University, 2019.(in Chinese)
[12] FONTANESI L.Merging metabolomics, genetics, and genomics in livestock to dissect complex production traits[M]//KADARMIDEEN H N.Systems biology in animal production and health, Vol.1.Cham:Springer, 2016:43-62.
[13] CLEMMONS B A, MIHELIC R I, BECKFORD R C, et al.Serum metabolites associated with feed efficiency in black Angus steers[J].Metabolomics, 2017, 13(12):147.
[14] KOCH R M, SWIGER L A, CHAMBERS D, et al.Efficiency of feed use in beef cattle[J].Journal of Animal Science, 1963, 22(2):486-494.  
[15] FRAGA C G, CLOWERS B H, MOORE R J, et al.Signature-discovery approach for sample matching of a nerve-agent precursor using liquid chromatography-mass spectrometry, XCMS, and chemometrics[J].Analytical Chemistry, 2010, 82(10):4165-4173.  
[16] ELOLIMY A A, ABDELMEGEID M K, MCCANN J C, et al.Residual feed intake in beef cattle and its association with carcass traits, ruminal solid-fraction bacteria, and epithelium gene expression[J].Journal of Animal Science and Biotechnology, 2018, 9:67.
[17] 卢果, 汪江山, 赵欣捷, 等.超高效液相色谱/飞行时间质谱法分析尿液中的代谢物用于区分人类性别的研究[J].色谱, 2006, 24(2):109-113. LU G, WANG J S, ZHAO X J, et al.Analysis of metabolites in urine by ultra performance liquid chromatography/time of flight mass spectrometry for the identification of human sex[J].Chinese Journal of Chromatography, 2006, 24(2):109-113.(in Chinese)
[18] 陈建军."联合代谢组学"筛选抑郁症性别差异性诊断标志物[D].博士学位论文.重庆:重庆医科大学, 2016. CHEN J J.Sex-specific urinary biomarkers for major depressive disorder diagnosis:identified by the combined application of GC-MS-and NMR-based metabonomics[D].Ph.D.Thesis.Chongqing:Chongqing Medical University, 2016.(in Chinese)
[19] TAKESHITA A, KOIBUCHI N, OKA J, et al.Bisphenol-A, an environmental estrogen, activates the human orphan nuclear receptor, steroid and xenobiotic receptor-mediated transcription[J].European Journal of Endocrinology, 2001, 145(4):513-517.
[20] KIM J E, JEON S M, PARK K H, et al.Does Glycine max leaves or Garcinia cambogia promote weight-loss or lower plasma cholesterol in overweight individuals:a randomized control trial[J].Nutrition Journal, 2011, 10:94.
[21] CHENG L C, TAN V M, GANESAN S, et al.Integrating phosphoproteomics into the clinical management of prostate cancer[J].Clinical and Translational Medicine, 2017, 6(1):9.
[22] 朱钦士.正转和反转的三羧酸循环[J].生物学通报, 2015, 50(1):16-19. ZHU Q S.The tricarboxylic acid cycle of positive and reverse[J].Bulletin of Biology, 2015, 50(1):16-19.(in Chinese)
[23] 梁红秀.剧烈运动对大鼠血浆嘌呤核苷酸代谢的影响[J].山东体育学院学报, 2005, 21(4):66-67, 71. LIANG H X.Effects of high intensity exercise on purine nucleotide metabolism of rats blood plasma[J].Journal of Shandong Institute of Physical Education and Sports, 2005, 21(4):66-67, 71.(in Chinese)
文章导航

/