水产动物营养与饲料 AQUATIC ANIMAL NUTRITION AND FEED

鱼溶浆替代鱼粉对罗氏沼虾生长性能、体组成、免疫指标和肠道消化酶活性的影响

  • 罗巧华 ,
  • 赵雅洁 ,
  • 李洪琴 ,
  • 顾夕章 ,
  • 丁桂涛 ,
  • 王光花 ,
  • 徐奇友 ,
  • 刘匆
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  • 1. 湖州师范学院生命科学学院, 水生动物繁育与营养国家地方联合工程实验室, 浙江省水生生物资源养护与开发技术研究重点实验室, 湖州 313000;
    2. 新希望六和股份有限公司, 成都 610011
罗巧华(1997-),女,福建永安人,硕士研究生,水产养殖专业。E-mail:841769458@qq.com

收稿日期: 2021-04-22

  网络出版日期: 2021-11-10

基金资助

国家自然科学基金项目(31972800)

Effects of Replacement of Fish Meal by Stickwater on Growth Performance, Body Composition, Immune Indexes and Intestinal Digestive Enzyme Activities of Macrobrachium rosenbergii

  • LUO Qiaohua ,
  • ZHAO Yajie ,
  • LI Hongqin ,
  • GU Xizhang ,
  • DING Guitao ,
  • WANG Guanghua ,
  • XU Qiyou ,
  • LIU Cong
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  • 1. Zhejiang Provincial Key Laboratory of Aquatic Bioresource Conservation and Development Technology, Nation Local Joint Engineering Laboratory of Aquatic Animal Genetic Breeding and Nutrition, College of Life Science, Huzhou University, Huzhou 313000, China;
    2. New Hope Liuhe Co., Ltd., Chengdu 610011, China

Received date: 2021-04-22

  Online published: 2021-11-10

Supported by

 

摘要

本试验旨在研究鱼溶浆替代鱼粉对罗氏沼虾生长性能、体组成、免疫指标和肠道消化酶活性的影响。选取健康、初始体重为(4.99±0.19) g的罗氏沼虾160尾,随机分为4组,每组4个重复,每个重复10尾虾。对照组(FM组)饲喂添加30%鱼粉的基础饲料,试验组分别饲喂用鱼溶浆替代20%(T20组)、40%(T40组)和60%(T60组)鱼粉的试验饲料。试验期43 d。结果表明:1)各组之间罗氏沼虾的增重率(WGR)、特定生长率(SGR)、成活率(SR)和饲料系数(FCR)均无显著差异(P>0.05)。2)各组之间罗氏沼虾的水分、粗蛋白质、粗灰分、粗脂肪含量均无显著差异(P>0.05)。3)各组之间罗氏沼虾的血淋巴超氧化物歧化酶(SOD)、溶菌酶(LZM)活性和丙二醛(MDA)含量以及肝胰腺酸性磷酸酶(ACP)活性均无显著差异(P>0.05)。T40组的肝胰腺碱性磷酸酶(AKP)活性显著低于对照组(P<0.05)。4)各组之间罗氏沼虾的肠道胰蛋白酶活性无显著差异(P>0.05)。T60组的肠道淀粉酶活性显著高于T20组(P<0.05)。T60组的肠道脂肪酶活性显著低于对照组和T20组(P<0.05)。由此可见,在本试验条件下,鱼溶浆替代20%~60%的鱼粉对罗氏沼虾的生长性能和体组成无显著影响,鱼溶浆替代60%的鱼粉会降低罗氏沼虾肠道脂肪酶活性。

本文引用格式

罗巧华 , 赵雅洁 , 李洪琴 , 顾夕章 , 丁桂涛 , 王光花 , 徐奇友 , 刘匆 . 鱼溶浆替代鱼粉对罗氏沼虾生长性能、体组成、免疫指标和肠道消化酶活性的影响[J]. 动物营养学报, 2021 , 33(11) : 6366 -6375 . DOI: 10.3969/j.issn.1006-267x.2021.11.036

Abstract

The purpose of this experiment was to study the effects of replacement of fish meal by stickwater on growth performance, body composition, immune indexes and intestinal digestive enzyme activities of Macrobrachium rosenbergii. One hundred and sixty healthy Macrobrachium rosenbergii with an initial body weight of (4.99±0.19) g were randomly divided into 4 groups with 4 replicates in each group and 10 prawns in each replicate. The control group (FM group) was fed a basal diet with 30% fish meal, and the experimental groups were fed the experimental diets which used stickwater replace 20% (T20 group), 40% (T40 group) and 60% (T60 group) fishmeal, respectively. The experiment lasted for 43 days. The results showed as follows:1) there were no significant differences in the weight gain rate (WGR), specific growth rate (SGR), survival rate (SR) and feed conversion rate (FCR) of Macrobrachium rosenbergii among all groups (P>0.05). 2) There were no significant differences in the contents of moisture, crude protein, ash and crude lipid of Macrobrachium rosenbergii among all groups (P>0.05). 3) There were no significant differences in the superoxide dismutase (SOD), lysozyme (LZM) activities and malondialdehyde (MDA) content in hemolymph and hepatopancreas acid phosphatase (ACP) activity of Macrobrachium rosenbergii among all groups (P>0.05). The hepatopancreas alkaline phosphate (AKP) activity of T40 group was significantly lower than that of the control group (P<0.05). 4) There was no significant difference in intestinal trypsin activity of Macrobrachium rosenbergii among all groups (P>0.05). The intestinal amylase activity of T60 group was significantly higher than that of T20 group (P<0.05). The intestinal lipase activity of T60 group was significantly lower than that of control group and T20 group (P<0.05). Therefore, under the experimental conditions, replacing 20% to 60% fish meal with stickwater has no effects on the growth performance and body composition, and replacing 60% fish meal with stickwater can decrease the intestinal lipase activity of Macrobrachium rosenbergii.

参考文献

[1] SKALLI A, ZAMBONINO-INFANTE J L, KOTZAMANIS Y, et al.Peptide molecular weight distribution of soluble protein fraction affects growth performance and quality in European sea bass (Dicentrarchus labrax) larvae[J].Aquaculture Nutrition, 2014, 20(2):118-131.  
[2] GOOSEN N J, DE WET L F, GÖRGENS J F.The effects of protein hydrolysates on the immunity and growth of the abalone Haliotis midae[J].Aquaculture, 2014, 428/429:243-248.
[3] HERNÁNDEZ C, OLVERA-NOVOA M A, SMITH D M, et al.Enhancement of shrimp Litopenaeus vannamei diets based on terrestrial protein sources via the inclusion of tuna by-product protein hydrolysates[J].Aquaculture, 2011, 317(1/4):117-123.
[4] SHAHIDI F, JANAK KAMIL Y V A.Enzymes from fish and aquatic invertebrates and their application in the food industry[J].Trends in Food Science & Technology, 2001, 12(12):435-464.  
[5] BUI H T D, KHOSRAVI S, FOURNIER V, et al.Growth performance, feed utilization, innate immunity, digestibility and disease resistance of juvenile red seabream (Pagrus major) fed diets supplemented with protein hydrolysates[J].Aquaculture, 2014, 418/419:11-16.
[6] 王晓艳, 马季, 王际英, 等.鱼溶浆粉在珍珠龙胆石斑鱼幼鱼的高植物蛋白饲料中的应用[J].中国海洋大学学报(自然科学版), 2021, 51(1):31-43. WANG X Y, MA J, WANG J Y, et al.Studies on supplementation of stickwater meal to high plant protein diets of juvenile Epinephelus fuscoguttatus ♀×E. lanceolatus pearl gentian[J].Periodical of Ocean University of China (Natural Science Edition), 2021, 51(1):31-43.(in Chinese)
[7] 王春利, 张海玲, 石磊, 等.鱼溶浆对大棚养殖南美白对虾生长的影响[J].安徽农业科学, 2018, 46(36):70-73, 77. WANG C L, ZHANG H L, SHI L, et al.Effect of fish soluble pulp on growth Penaeus vannamei in greenhouse[J].Journal of Anhui Agricultural Sciences, 2018, 46(36):70-73, 77.(in Chinese)
[8] SHI Y, ZHONG L, MA X K, et al.Effect of replacing fishmeal with stickwater hydrolysate on the growth, serum biochemical indexes, immune indexes, intestinal histology and microbiota of rice field eel (Monopterus albus)[J].Aquaculture Reports, 2019, 15:100223.
[9] 骆艺文, 艾庆辉, 麦康森, 等.饲料中添加牛磺酸和胆固醇对军曹鱼生长、体组成和血液指标的影响[J].中国海洋大学学报(自然科学版), 2013, 43(8):31-36. LUO Y W, AI Q H, MAI K S, et al.Effects of dietary taurine and cholesterol on growth performance, body composition and plasma metabolites in diets of cobia (Rachycentron canadum L.)[J].Periodical of Ocean University of China, 2013, 43(8):31-36.(in Chinese)
[10] KIM S K, MATSUNARI H, TAKEUCHI T, et al.Effect of different dietary taurine levels on the conjugated bile acid composition and growth performance of juvenile and fingerling Japanese flounder Paralichthys olivaceus[J].Aquaculture, 2007, 273(4):595-601.  
[11] 周铭文, 王和伟, 叶继丹.饲料牛磺酸对尼罗罗非鱼生长、体成分及组织游离氨基酸含量的影响[J].水产学报, 2015, 39(2):213-223. ZHOU M W, WANG H W, YE J D.Effects of taurine supplementation on the growth, body composition and tissue free amino acid concentrations in Nile tilapia (Oreochromis niloticus)[J].Journal of Fisheries of China, 2015, 39(2):213-223.
[12] 王际英, 姜柯君, 夏斌, 等.小肽对星斑川鲽幼鱼消化酶活性、抗氧化能力和生化组成的影响[J].中国水产科学, 2014, 21(6):1154-1164. WANG J Y, JIANG K J, XIA B, et al.Effects of small dietary peptides on digestive enzyme activity, anti-oxidative capability, and biochemical composition in tissues of juvenile starry flounder Platichthys stellatus[J].Journal of Fishery Sciences of China, 2014, 21(6):1154-1164.(in Chinese)
[13] MENDOZA R, MONTEMAYOR J, VERDE J.Biogenic amines and pheromones as feed attractants for the freshwater prawn Macrobrachium rosenbergii[J].Aquaculture Nutrition, 1997, 3(3):167-173.  
[14] 周露阳.酶解虾浆、酶解鱼浆(粉)、鱼溶浆、酶解鱼溶浆和酶解鱼粉对黄颡鱼(Pelteobagrus fulvidraco)生长的影响[D].硕士学位论文.苏州:苏州大学, 2019. ZHOU L Y.Effects of dietary shrimp protein hydrolysates, fish protein hydrolysates (meal), stickwater, stickwater hydrolysate and fish meal hydrolysates for yellow catfish (Pelteobagrus fulvidraco) on growth performance and physiological health[D].Master's Thesis.Suzhou:Soochow University, 2019.(in Chinese)
[15] 叶陈静, 杨霞, 沈小燕, 等.五种诱食剂对罗氏沼虾生长性能及消化酶活性的影响[J].湖州师范学院学报, 2016, 38(8):75-80. YE C J, YANG X, SHEN X Y, et al.Effects of five attractants on growth performance and digestive enzyme activities of freshwater prawn, Macrobrachium rosenbergii[J].Journal of Huzhou Teachers College, 2016, 38(8):75-80.(in Chinese)
[16] 高敏敏, 吴代武, 周露阳, 等.饲料鱼溶浆影响黄颡鱼的胆汁酸代谢及脂肪沉积[J].水生生物学报, 2019, 43(4):731-738. GAO M M, WU D W, ZHOU L Y, et al.Effects of stickwater on bile acid and lipid metabolism of yellow catfish (Pelteobagrus fulvidraco)[J].Acta Hydrobiologica Sinica, 2019, 43(4):731-738.(in Chinese)
[17] 周露阳, 吴代武, 高敏敏, 等.鱼溶浆、酶解鱼溶浆和酶解鱼浆完全替代鱼粉对黄颡鱼生长的影响[J].水生生物学报, 2019, 43(3):504-516. ZHOU L Y, WU D W, GAO M M, et al.The effects of fish meal replacement on growth performance of yellow catfish (Pelteobagrus fulvidraco)[J].Acta Hydrobiologica Sinica, 2019, 43(3):504-516.(in Chinese)
[18] 常志强, 李金宝, 李健, 等.大菱鲆、褐牙鲆和半滑舌鳎的血液生化指标特征[J].中国渔业质量与标准, 2013, 3(4):76-82. CHANG Z Q, LI J B, LI J, et al.Characteristics of blood biochemical parameters in turbot, olive flounder and tongue sole[J].Chinese Fishery Quality and Standards, 2013, 3(4):76-82.(in Chinese)
[19] 徐靖.超氧化物歧化酶及其应用的研究进展[J].食品工业科技, 2013, 34(12):387-391. XU J.Research progress in superoxide dismutase and its application[J].Science and Technology of Food Industry, 2013, 34(12):387-391.(in Chinese)
[20] RENGPIPAT S, RUKPRATANPORN S, PIYATIRATITIVORAKUL S, et al.Immunity enhancement in black tiger shrimp (Penaeus monodon) by a probiont bacterium (Bacillus S11)[J].Aquaculture, 2000, 191(4):271-288.  
[21] AI Q H, MAI K S, TAN B P, et al.Replacement of fish meal by meat and bone meal in diets for large yellow croaker, Pseudosciaena crocea[J].Aquaculture, 2006, 260(1/2/3/4):255-263.
[22] TAN B P, MAI K S, ZHENG S X, et al.Replacement of fish meal by meat and bone meal in practical diets for the white shrimp Litopenaeus vannamai (Boone)[J].Aquaculture Research, 2005, 36(5):439-444.  
[23] 曹小华, 黄中业, 陈宇飞, 等.三种水产诱食剂的营养价值研究与安全性评价[J].广东饲料, 2021, 30(2):26-30. CAO X H, HUANG Z Y, CHEN Y F, et al.Study on the nutritional value and safety evaluation of three aquatic food attractants[J].Guangdong Feed, 2021, 30(2):26-30.(in Chinese)
[24] 刘树青, 江晓路, 牟海津, 等.免疫多糖对中国对虾血清溶菌酶、磷酸酶和过氧化物酶的作用[J].海洋与湖沼, 1999, 30(3):278-283. LIU S Q, JIANG X L, MOU H J, et al.Effects of immunopoiysaccharide on LSZ, ALP, ACP and POD activities of Peneusis chinensis serum[J].Oceanologia et Limnologia Sinica, 1999, 30(3):278-283.(in Chinese)
[25] 陈清西, 陈素丽, 石艳, 等.长毛对虾碱性磷酸酶性质[J].厦门大学学报(自然科学版), 1996(2):257-261. CHEN Q X, CHEN S L, SHI Y, et al.Characterization of alkaline phosphatase from Penaeus penicillatus[J].Journal of Xiamen University(Natural Science Edition), 1996(2):257-261.(in Chinese)
[26] 于彬彬, 贾志武, 张晓峰, 等.镜鲤酸性磷酸酶的QTL分析[J].水产学杂志, 2012, 25(3):15-19. YU B B, JIA Z W, ZHANG X F, et al.Quantitative trait loci analysis for acid phosphatase in mirror carp[J].Chinese Journal of Fisheries, 2012, 25(3):15-19.(in Chinese)
[27] 孙建梅, 张士璀, 王勇军.酸性磷酸酶在文昌鱼体内的分布[J].海洋水产研究, 2006, 27(4):17-20. SUN J M, ZHANG S C, WANG Y J, et al.Distribution of acid phosphatase in amphioxus Branchiostoma belcheri tsingtauense[J].Marine Fisheries Research, 2006, 27(4):17-20.(in Chinese)
[28] 王婧瑶, 吴莉芳, 王东, 等.豆粕替代鱼粉对黄金鲫生长、消化酶活力及非特异性免疫指标的影响[J/OL].吉林农业大学学报:1-8.(2020-09-10)[2021-03-29].https://doi.org/10.13327/j.jjlau.2020.5161. WANG J Y, WU L F, WANG D, et al.Effects of soybean meal instead of fish meal on growth, digestive enzyme activity and non-specific immune indexes of Carassius auratus[J/OL].Joumal of Jilin Agricultural University:1-8.(2020-09-10)[2021-03-29].https://doi.org/10.13327/j.jjlau.2020.5161.(in Chinese)
[29] 向枭, 周兴华, 陈建, 等.饲料中豆粕蛋白替代鱼粉蛋白对齐口裂腹鱼幼鱼生长性能、体成分及血液生化指标的影响[J].水产学报, 2012, 36(5):723-731. XIANG X, ZHOU X H, CHEN J, et al.Effect of dietary replacement of fish meal protein with soybean meal protein on the growth, body composition and hematology indices of Schizothorax prenanti[J].Journal of Fisheries of China, 2012, 36(5):723-731.(in Chinese)
[30] 方卫东, 鲁康乐, 张春晓, 等.豆粕替代鱼粉对牛蛙生长、体组成、消化酶活力及肝脏生化指标的影响[J].水产学报, 2016, 40(11):1742-1752. FANG W D, LU K L, ZHANG C X, et al.Effects of fish meal replacement by soybean meal on growth, body composition, digestive enzyme activities and hepatic biochemical indices of Rana (Lithobates) catesbeiana[J].Journal of Fisheries of China, 2016, 40(11):1742-1752.(in Chinese)
[31] 安贸麟, 范泽, 王庆奎, 等.豆粕替代鱼粉对点带石斑鱼生长、消化和抗氧化能力的影响[J].江苏农业科学, 2018, 46(16):128-132. AN M L, FAN Z, WANG Q K, et al.Influences of dietary replacement of fishmeal by soybean meal on growth performance, digestive ability and antioxidant ability of Epinephelus malabaricus[J].Jiangsu Agricultural Sciences, 2018, 46(16):128-132.(in Chinese)
[32] 赵柳兰, 龙亚男, 罗杰, 等.池塘和稻田两种养殖模式下建鲤肠道菌群、免疫酶活性及肌肉氨基酸比较分析[J].中国水产科学, 2021, 28(1):48-56. ZHAO L L, LONG Y N, LUO J, et al.Analysis and comparison of intestinal microbiota, immune enzyme activities, and muscle flavor of Jian carp in two culture modes[J].Journal of Fishery Sciences of China, 2021, 28(1):48-56.(in Chinese)
[33] 凌云.牛磺酸对虎纹蛙(Hoplobatrachus rugulosa)非特异性免疫、消化酶活力及抗氧化能力的影响[D].硕士学位论文.金华:浙江师范大学, 2012. LING Y.The effect of taurine on the nonspecific immune response, digestive enzyme activity and antioxidant ability in the tiger frog (Hoplobatrachus rugulosa)[D].Master's Thesis.Jinhua:Zhejiang Normal University, 2012.(in Chinese)
[34] 高春生, 范光丽, 王艳玲.牛磺酸对黄河鲤鱼生长性能和消化酶活性的影响[J].中国农学通报, 2007, 23(6):645-647. GAO C S, FAN G L, WANG Y L.Effects of taurine on growth performance and digestive enzyme activity of carp[J].Chinese Agricultural Science Bulletin, 2007, 23(6):645-647.(in Chinese)
[35] 周婧, 王旭, 刘霞, 等.饲料中牛磺酸水平对红鳍东方鲀免疫及消化酶的影响[J].大连海洋大学学报, 2019, 34(1):101-108. ZHOU J, WANG X, LIU X, et al.Effects of dietary taurine levels on immunity and digestive enzyme activities of redfin puffer Takifugu rubripes[J].Journal of Dalian Fisheries University, 2019, 34(1):101-108.(in Chinese)
[36] 何明, 刘利平, 曲恒超, 等.牛磺酸对花鳗鲡生长和消化酶活力的影响[J].上海海洋大学学报, 2017, 26(2):227-234. HE M, LIU L P, QU H C, et al.Effects of dietary taurine on growth performance and digestive enzyme activity of Anguilla marmorata[J].Journal of Shanghai Ocean University, 2017, 26(2):227-234.(in Chinese)
[37] 李昭林.低鱼粉饲料中添加牛磺酸对黄鳝生长、免疫及代谢的影响[D].硕士学位论文.长沙:湖南农业大学, 2016. LI Z L.Effect of taurine on growth, immune and metabolism in rice filed eel (Monopterus albus) fed low fish meal[D].Master's Thesis.Changsha:Hunan Agricultural University, 2016.(in Chinese)
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