分子与细胞营养 MOLECULAR AND CELLULAR NUTRITION

青蒿素对奶牛乳腺上皮细胞乳脂合成相关基因表达的影响

  • 侯昆 ,
  • 李欣 ,
  • 沈义媛 ,
  • 詹经纬 ,
  • 牛慧 ,
  • 熊本海 ,
  • 童津津 ,
  • 蒋林树
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  • 1. 北京农学院动物科学技术学院, 奶牛营养学北京市重点实验室, 北京 102206;
    2. 中国农业科学院北京畜牧兽医研究所, 北京 100193
侯昆(1996-),男,北京人,硕士研究生,研究方向为反刍动物营养与免疫。E-mail:1031520946@qq.com

收稿日期: 2021-04-17

  网络出版日期: 2021-11-10

基金资助

国家自然科学基金(31802091);北京市教委重点项目(20JF0008)

Effects of Artemisinin on Expression of Milk Fat Synthesis Related Genes in Mammary Epithelial Cells of Dairy Cows

  • HOU Kun ,
  • LI Xin ,
  • SHEN Yiyuan ,
  • ZHAN Jingwei ,
  • NIU Hui ,
  • XIONG Benhai ,
  • TONG Jinjin ,
  • JIANG Linshu
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  • 1. Beijing Key Laboratory of Dairy Cow Nutrition, College of Animal Science and Technology, Beijing University of Agriculture, Beijing 102206, China;
    2. Beijing Institute of Animal Husbandry and Veterinary Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China

Received date: 2021-04-17

  Online published: 2021-11-10

Supported by

 

摘要

本试验旨在研究青蒿素对奶牛乳腺上皮细胞(BMECs)乳脂合成相关基因表达的影响。采用单因素完全随机试验设计,添加青蒿素浓度分别为0(对照)、10、20、40、60、80、100 μmol/L的培养基与BMECs共培养1、3、6、9、12和24 h,每组6个重复。结果表明:与对照组相比,作用12 h后,青蒿素浓度为20、40、60 μmol/L极显著提高了BMECs的相对活性(P<0.01),显著提高了脂质的聚集和细胞内甘油三酯(TG)的含量(P<0.05);实时荧光定量PCR结果表明,青蒿素能显著提高乳脂合成相关基因过氧化物酶体增殖物激活受体γ(PPARγ)、胆固醇调节元件结合蛋白1-c (SREBP1-c)、脂肪酸合成酶(FASN)和乙酰辅酶A羧化酶(ACC)基因相对表达量(P<0.05);青蒿素也可极显著提高丝氨酸/苏氨酸蛋白激酶1(AKT1)、哺乳动物雷帕霉素靶蛋白(mTOR)和硬脂酰辅酶A去饱和酶1(SCD1)基因相对表达量(P<0.01);Western-blot结果表明,40 μmol/L的青蒿素显著上调了腺苷酸活化蛋白激酶(AMPK)、mTOR、磷酸化哺乳动物雷帕霉素靶蛋白(p-mTOR)、PPARγ、SREBP1-c蛋白的表达(P<0.05)。综上所述,40 μmol/L的青蒿素提高BMECs乳脂合成的效果较好,青蒿素通过调控AMPK-mTOR信号通路中关键基因的转录从而促进BMECs中乳脂肪的合成。

本文引用格式

侯昆 , 李欣 , 沈义媛 , 詹经纬 , 牛慧 , 熊本海 , 童津津 , 蒋林树 . 青蒿素对奶牛乳腺上皮细胞乳脂合成相关基因表达的影响[J]. 动物营养学报, 2021 , 33(11) : 6407 -6419 . DOI: 10.3969/j.issn.1006-267x.2021.11.040

Abstract

This experiment was conducted to investigate the effects of artemisinin on expressions of genes related to milk fat in bovine mammary epithelial cells (BEMCs). The experiment was designed as a single factor randomized trial, cells were cultured in culture medium supplemented with 0 (control), 20, 40, 60, 80 and 100 μmol/L artemisinin for 1, 3, 6, 9, 12 and 24 h, with 6 replicates per group. The results showed that compared with the control group, 20、40 and 60 μmol/L artemisinin could significantly increase the relative activity of BEMCs (P<0.01), significantly increase lipid aggregation and triglyceride (TG) content (P<0.05); real-time fluorescent quantitative PCR results showed that artemisinin could significantly increase milk fat synthesis-related genes peroxisome proliferator-activated receptor γ (PPARγ), sterol regulatory element-binding protein 1-c (SREBP1-c), fatty acid synthase (FASN) and acetyl-CoA carboxylase (ACC) relative expression levels (P<0.05); artemisinin could also significantly increase the relative expression levels of serine/threonine protein kinases 1 (AKT1), mammalian target of rapamycin (mTOR) and stearoyl CoA desaturase 1 (SCD1) genes (P<0.01); Western-blot results showed that 40 μmol/L artemisinin significantly up-regulated the expression of AMPK, mTOR, phosphorylated mammalian target of rapamycin (p-mTOR), PPARγ and SREBP1-c protein (P<0.05). Combined with the above results, the promotion effects of 40 μmol/L artemisinin on improving the milk fat synthesis of BMECs are better, artemisinin promotes the synthesis of milk fat in BMECs by regulating the transcription and translation of key genes in the AMPK-mTOR signaling pathway.

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