分子与细胞营养 MOLECULAR AND CELLULAR NUTRITION

新生荷斯坦犊牛后肠道微生物母源传递特征的研究

  • 杨敏娜 ,
  • 朱焕 ,
  • 高伟星 ,
  • 曲永利
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  • 黑龙江八一农垦大学动物科技学院, 大庆 163000
杨敏娜(1995-),女,山西运城人,硕士研究生,动物营养与饲料科学专业。E-mail:419324426@qq.com

收稿日期: 2021-05-16

  网络出版日期: 2021-11-10

Study on Maternal Transmission Characteristics of Hindgut Microorganism in Newborn Holstein Calves

  • YANG Minna ,
  • ZHU Huan ,
  • GAO Weixing ,
  • QU Yongli
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  • College of Animal Science and Technology, Heilongjiang Bayi Agricultural University, Daqing 163000, China

Received date: 2021-05-16

  Online published: 2021-11-10

摘要

本试验旨在探讨新生荷斯坦犊牛后肠道微生物区系的母体来源。利用16S rDNA扩增子测序技术分析6对荷斯坦母牛的胎盘、脐带、羊水、初乳、粪便和犊牛胎粪的微生物区系特征,结合SourceTracker方法研究母牛不同部位微生物对犊牛后肠道菌群的定植贡献。结果表明:所有样本类型都有特异性操作分类单元(OTU)。羊水和初乳、胎盘和脐带微生物区系结构相似;母牛粪便和犊牛胎粪具有独特的微生物区系。母牛的胎盘、脐带、羊水、初乳和粪便中的微生物均是新生犊牛胎粪微生物的主要来源,按贡献比例由高到低依次为脐带(23.45%)、胎盘(16.35%)、初乳(14.29%)、羊水(11.45%)、母牛粪便(9.93%)。本试验证明,围产期母牛胎盘、脐带、羊水、初乳和粪便中的微生物可通过母源传递方式在犊牛肠道中进行定植,其中脐带微生物对胎粪微生物的定植贡献最大。犊牛胎粪中还有许多未知来源且相对贡献度(24.53%)较高的微生物。

本文引用格式

杨敏娜 , 朱焕 , 高伟星 , 曲永利 . 新生荷斯坦犊牛后肠道微生物母源传递特征的研究[J]. 动物营养学报, 2021 , 33(11) : 6445 -6451 . DOI: 10.3969/j.issn.1006-267x.2021.11.043

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