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高产漆酶菌株的筛选、鉴定和固态发酵条件优化

  • 李彩联 ,
  • 郭艳丽 ,
  • 张铁鹰 ,
  • 王丽华 ,
  • 高爱琴
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  • 1. 甘肃农业大学动物科学技术学院, 兰州 730070;
    2. 中国农业科学院北京畜牧兽医研究所, 动物营养学国家重点实验室, 北京 100193;
    3. 内蒙古农业大学动物科学技术学院, 呼和浩特 010018
李彩联(1994-),女,甘肃天水人,硕士研究生,研究方向为酶与抗营养因子。E-mail:2279384898@qq.com

收稿日期: 2021-04-08

  网络出版日期: 2021-11-10

基金资助

中国农业科学院科技创新工程专项(ASTPI-IASO8)

Screening, Identification and Optimization of Solid-State Fermentation Conditions for High-Yield Laccase Strain

  • LI Cailian ,
  • GUO Yanli ,
  • ZHANG Tieying ,
  • WANG Lihua ,
  • GAO Aiqin
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  • 1. College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China;
    2. State Key Laboratory of Animal Nutrition, Beijing Institute of Animal Husbandry and Veterinary Medicine, Chinese Academy of Agricultural Sciences, Beijing 100193, China;
    3. College of Animal Science and Technology, Inner Mongolia Agricultural University, Hohhot 010018, China

Received date: 2021-04-08

  Online published: 2021-11-10

Supported by

 

摘要

本试验旨在筛选高产漆酶菌株,并对其固态发酵条件进行优化。利用平板显色和酶活性测定的方法,从33株木腐菌(白腐菌和褐腐菌)中筛选出1株高产漆酶菌株,采用单因素试验和正交试验设计对其产漆酶固态发酵条件进行优化。结果表明:33株木腐菌中25号菌生长最快,发酵底物中漆酶活性最高。经分子生物学鉴定,确定该菌株为灵芝属。经对培养基含水量、接种量、通氧量和发酵时间等发酵条件进行单因素试验和正交试验,最终获得其最优固态发酵条件为10 g麸皮、62.5%水分、5%接种量、通氧量15%、培养温度30℃、培养时间120 h。该菌株经发酵条件优化后,漆酶活性达9 522.22 U/g,较优化前(3 111.53 U/g)提高了2.06倍。

本文引用格式

李彩联 , 郭艳丽 , 张铁鹰 , 王丽华 , 高爱琴 . 高产漆酶菌株的筛选、鉴定和固态发酵条件优化[J]. 动物营养学报, 2021 , 33(11) : 6501 -6509 . DOI: 10.3969/j.issn.1006-267x.2021.11.049

Abstract

The purpose of this experiment was to screen the high-yield laccase strains and optimize their solid-state fermentation conditions. A high-yield laccase strain was selected from 33 wood rot fungi (white rot and brown rot) by plate coloration and enzyme activity determination. The solid state fermentation conditions of laccase were optimized by single factor method and orthogonal test design. The results showed that the strain 25 in 33 strains of wood rot fungi grew fastest, the laccase activity was highest in the fermentation substrate. Identified by molecular biology, the strain was identified as Ganoderma lucidum. Through the single factor and orthogonal test of fermentation conditions such as water content, inoculation amount, oxygen content and fermentation time, the optimum fermentation conditions were 100% bran, 72.5% water content, 20% inoculation amount, 15% oxygen content,30℃ culture temperature, 90 h fermentation time. The enzyme activity of the strain is 9 522.22 U/g after optimization of fermentation conditions, which is 2.06 times higher than that before optimization (3 111.53 U/g).

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