动物营养学报 >

2021 , Vol. 33 >Issue 12: 6699 - 6708

DOI: https://doi.org/10.3969/j.issn.1006-267x.2021.12.012

猪营养与饲料 SWINE NUTRITION AND FEED

大豆异黄酮对体外氧化应激仔猪肠上皮细胞的影响及机制研究

  • 胡胜兰 ,
  • 肖昊 ,
  • 王丽 ,
  • 黄琳 ,
  • 蒋宗勇
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  • 广东省农业科学院动物科学研究所, 畜禽育种国家重点实验室, 农业农村部华南动物营养与饲料重点实验室, 岭南现代农业科学与技术广东省实验室茂名分中心, 广东省畜禽育种与营养研究重点实验室, 广州 510640
胡胜兰(1984-),女,湖北随州人,副研究员,博士,从事仔猪营养与免疫调控研究。E-mail:hushenglan@gdaas.cn

收稿日期: 2021-05-13

  网络出版日期: 2021-12-16

基金资助

国家自然科学基金项目（31902172）；财政部和农业农村部国家现代农业产业技术体系（CARS-35）；广东省现代农业产业技术体系生猪创新团队项目（2020KJ126）；广东省农业科学院科技计划项目（R2020PY-JG009）

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Effects and Mechanism Research of Soybean Isoflavones on Intestinal Epithelial Cells of Piglets under Oxidative Stress in Vitro

  • HU Shenglan ,
  • XIAO Hao ,
  • WANG Li ,
  • HUANG Ling ,
  • JIANG Zongyong
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  • Guangdong Key Laboratory of Animal Breeding and Nutrition, Maoming Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Key Laboratory of Animal Nutrition and Feed Science in South China, Ministry of Agriculture and Rural Affairs, State Key Laboratory of Livestock and Poultry Breeding, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China

Received date: 2021-05-13

  Online published: 2021-12-16

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摘要

本试验旨在揭示大豆异黄酮对氧化应激条件下仔猪肠上皮细胞抗氧化能力和免疫性能的影响。试验在FeSO4/H2O2建立仔猪肠上皮细胞氧化应激模型的基础上，分别加入不同浓度的大豆异黄酮处理48 h后，测定细胞内抗氧化酶活性、免疫细胞因子分泌以及相关信号通路。试验同时设正常对照组和氧化对照组。结果表明：与氧化对照组相比，添加10~40 μmol/L大豆异黄酮后显著提高了细胞增殖率（P<0.05），显著降低了细胞凋亡率（P<0.05）；与正常对照组相比，氧化处理显著提高了细胞内白细胞介素-1β（IL-1β）、白细胞介素-2（IL-2）和肿瘤坏死因子-α（TNF-α）含量（P<0.05），显著降低了细胞内白细胞介素-4（IL-4）含量（P<0.05）；10~80 μmol/L大豆异黄酮组细胞内TNF-α含量显著低于氧化对照组（P<0.05）；此外，氧化处理提高了细胞内核因子-κB（NF-κB）含量和Jun N-末端蛋白激酶（JNK）活性。添加20 μmol/L大豆异黄酮对细胞内诱导型一氧化氮合成酶（iNOS）、NF-κB蛋白的表达均有抑制作用。综上所述，添加大豆异黄酮可提高体外氧化应激仔猪肠上皮细胞的抗氧化能力，并通过调控NF-κB和JNK信号通路抑制氧化应激诱导的细胞炎症反应。

关键词： 大豆异黄酮; 仔猪肠上皮细胞; 抗氧化; 炎症反应; 信号通路

本文引用格式

胡胜兰 , 肖昊 , 王丽 , 黄琳 , 蒋宗勇 . 大豆异黄酮对体外氧化应激仔猪肠上皮细胞的影响及机制研究[J]. 动物营养学报, 2021 , 33(12) : 6699 -6708 . DOI: 10.3969/j.issn.1006-267x.2021.12.012

Abstract

This experiment was conducted to investigate the effects of soybean isoflavones (SI) on the antioxidant capacity and immune performance of intestinal epithelial cells of piglets under oxidative stress. Based on the oxidative stress model of piglets' intestinal epithelial cells established by FeSO4/H2O2, the antioxidant enzyme activities, immunological cytokine secretion and related signaling pathways were measured after 48 h treatment with different concentrations of SI. A normal control group and an oxidized control group were set at the same time. The results showed that, compared with the oxidized control group, the addition of 10 to 40 μmol/L SI significantly increased the cell proliferation rate (P<0.05), and significantly decreased the cell apoptosis rate (P<0.05). Compared with the normal control group, the contents of interleukin-1β (IL-1β), interleukin-2 (IL-2) and tumor necrosis factor-α (TNF-α) were significantly increased (P<0.05), and the content of interleukin-4 (IL-4) was significantly decreased by oxidation treatment (P<0.05). The addition of 10 to 80 μmol/L SI groups had a significantly lower TNF-α content than that of the oxidized control group (P<0.05). In addition, oxidation treatment increased the content of nuclear factor-κB (NF-κB) and the activity of Jun N-terminal kinase (JNK), and the addition of 10 to 20 μmol/L SI inhibited the protein expression of nitric oxide (NO), inducible nitric oxide synthase (iNOS), NF-κB and JNK in cells. In conclusion, the addition of SI can improve the antioxidant capacity of intestinal epithelial cells of piglets under oxidative stress in vitro, and inhibit the inflammatory response induced by oxidative stress by regulating NF-κB and JNK signaling pathways.

Key words： soybean isoflavones; intestinal epithelial cells of piglets; antioxidant; inflammatory response; signal pathway

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