动物营养学报 >

2021 , Vol. 33 >Issue 12: 6721 - 6729

DOI: https://doi.org/10.3969/j.issn.1006-267x.2021.12.014

猪营养与饲料 SWINE NUTRITION AND FEED

甜菜碱对宫内发育迟缓仔猪生长性能、肝脏损伤和抗氧化功能的影响

  • 李悦 ,
  • 张昊 ,
  • 陈亚楠 ,
  • 陈跃平 ,
  • 杨敏馨
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  • 1. 江苏省农业科学院畜牧研究所, 农业农村部种养结合重点实验室, 南京 210014;
    2. 南京农业大学动物科技学院, 南京 210095;
    3. 福建省畜牧总站, 福州 350003
李悦(1990-),女,四川西昌人,助理研究员,博士,研究方向为动物营养与饲料科学。E-mail:liyue9032@163.com

收稿日期: 2021-05-11

  网络出版日期: 2021-12-16

基金资助

国家自然科学基金项目（31902197，31802094）；江苏省自然科学基金项目（BK20180531）

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Effects of Betaine on Growth Performance, Liver Damage and Antioxidant Function of Intrauterine Growth Retarded Piglets

  • LI Yue ,
  • ZHANG Hao ,
  • CHEN Ya'nan ,
  • CHEN Yueping ,
  • YANG Minxin
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  • 1. Key Laboratory for Crop and Animal Integrated Farming of Ministry of Agriculture and Rural Affairs, Institute of Animal Science, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China;
    2. College of Animal Science & Technology, Nanjing Agricultural University, Nanjing 210095, China;
    3. Fujian Animal Husbandry Station, Fuzhou 350003, China

Received date: 2021-05-11

  Online published: 2021-12-16

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摘要

本试验旨在研究甜菜碱对宫内发育迟缓（IUGR）仔猪生长性能、肝脏损伤和抗氧化功能的影响。从6窝符合正常初生重（NBW）和IUGR选择标准的新生仔猪中，每窝选择1头NBW和2头IUGR雄性仔猪（共18头），自然哺乳至7日龄，随后分至NBW对照组、IUGR对照组和IUGR试验组，每组6个重复，每个重复1头。NBW对照组和IUGR对照组饲喂基础配方乳，IUGR试验组饲喂在基础配方乳中添加1.0 g/kg甜菜碱（以乳粉干物质计）的试验配方乳。试验期15 d。结果表明：1）与NBW对照组相比，IUGR显著降低了仔猪7和21日龄体重以及7~21日龄平均日增重（ADG）和平均日采食量（ADFI）（P<0.05）；2）IUGR对照组仔猪血浆丙氨酸氨基转移酶（ALT）和天门冬氨酸氨基转移酶（AST）活性较NBW对照组显著升高（P<0.05），IUGR试验组仔猪血浆ALT活性较IUGR对照组显著降低（P<0.05）；3）与NBW对照组相比，IUGR对照组仔猪肝细胞活性氧（ROS）水平和肝脏丙二醛（MDA）、蛋白羰基（PC）以及氧化型谷胱甘肽（GSSG）含量显著提高（P<0.05），而肝脏γ-谷氨酰半胱氨酸连接酶（γ-GCL）和谷胱甘肽还原酶（GR）活性以及总谷胱甘肽（T-GSH）、还原型谷胱甘肽（GSH）和S-腺苷蛋氨酸（SAM）含量显著降低（P<0.05）；4）与IUGR对照组相比，IUGR试验组仔猪肝细胞ROS水平和肝脏PC含量显著降低（P<0.05），肝脏γ-GCL活性以及T-GSH、GSH和SAM含量显著升高（P<0.05）。综上所述，添加1.0 g/kg甜菜碱可改善IUGR仔猪肝脏抗氧化功能，缓解氧化应激和组织损伤。

关键词： 宫内发育迟缓; 甜菜碱; 肝脏损伤; 氧化应激; 仔猪

本文引用格式

李悦 , 张昊 , 陈亚楠 , 陈跃平 , 杨敏馨 . 甜菜碱对宫内发育迟缓仔猪生长性能、肝脏损伤和抗氧化功能的影响[J]. 动物营养学报, 2021 , 33(12) : 6721 -6729 . DOI: 10.3969/j.issn.1006-267x.2021.12.014

Abstract

This study was conducted to investigate the effects of betaine on growth performance, liver damage and antioxidant function of intrauterine growth retarded (IUGR) piglets. One male normal birth weight (NBW) and two same-sex IUGR piglets (total 18 piglets) were chosen from each of six litters of newborn piglets that met the selection criteria for NBW and IUGR. All piglets were allowed to suck the dam naturally up to 7 days of age. After that, the NBW and IUGR piglets in each litter were assigned to the NBW control group, IUGR control group and IUGR experimental group with 6 replicates each and 1 piglet in each replicate, respectively. The NBW control group and IUGR control group received the basal formula milk, while the IUGR experimental group fed the basal formula milk supplemented with 1.0 g/kg betaine (as dry matter of milk powder basis). The trial lasted for 15 days. The results showed as follows:1) compared with the NBW control group, IUGR significantly decreased the body weights at 7 and 21 days of age, the average daily gain (ADG) and average daily feed intake (ADFI) of piglets from 7 to 21 days of age (P<0.05). 2) Compared with the NBW control group, the IUGR control group exhibited significantly higher activities of plasma alanine aminotransferase (ALT) and aspartate aminotransferase (AST) (P<0.05), whereas the IUGR experimental group showed a significantly decreased ALT activity in the plasma of piglets in comparison with the IUGR control group (P<0.05). 3) Compared with the NBW control group, the hepatocellular reactive oxygen species (ROS) level and the contents of malondialdehyde (MDA), protein carbonyl (PC) and oxidized glutathione (GSSG) in liver of piglets in the IUGR control group were significantly increased (P<0.05); in contrast, the activities of γ-glutamyl cysteine ligase (γ-GCL) and glutathione reductase (GR) and the contents of total glutathione (T-GSH), reduced glutathione (GSH), and S-adenosylmethionine (SAM) in liver of piglets were significantly decreased (P<0.05). 4) Compared with the IUGR control group, the hepatocellular ROS level and PC content in liver of piglets in the IUGR experimental group were significantly decreased (P<0.05), while the γ-GCL activity and contents of T-GSH, GSH and SAM were significantly increased (P<0.05). It is concluded that supplementation with betaine at a dose of 1.0 g/kg can improve the antioxidant function and alleviate the oxidative stress and tissue damage in liver of IUGR piglets.

Key words： intrauterine growth retardation; betaine; liver damage; oxidative stress; piglets

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